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We narrowed to 23,543 results for: CHI

Showing: 4901 - 4950 of 23543 results

  14. pLenti_dCas9-2xAM_hIRF-1

    Plasmid
    #92221
    Purpose
    Lentiviral plasmid expressing dCas9-2xAM and gRNA for human IRF-1 promoter
    Depositor
    Hodaka Fujii
    Article
    Fujita et al BMC Res Notes. 2018 Feb 27;11(1):154. do
    Inserts
    Sp-dCas9-2xAM tag
    gRNA targeting human IRF-1 promoter
    Use
    CRISPR and Lentiviral
    Tags
    2xAM tag
    Expression
    Mammalian
    Mutation
    human codon-optimized, D10A + H840A
    Promoter
    CBh and U6
    Available since
    June 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    92221-plasmid-map-sequence-id-181086

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  29. pHL 1917

    Plasmid
    #53024
    Purpose
    AmpR + PCon:RBS (st7)::mCherry::T1 terminator + PLlacO-1::RBS (st7) fimB::Asp terminator + ColE1
    Depositor
    Han Lim
    Article
    Hung et al Nat Commun. 2014 Aug 4;5:4574. doi: 10.1
    Inserts
    mCherry
    fimB (fimB E. coli)
    Use
    Synthetic Biology
    Tags
    Asp terminator, RBS(st7), and T1 terminator
    Expression
    Bacterial
    Mutation
    Promoter
    pCon and pLlacO-1
    Available since
    May 16, 2014
    Availability
    Academic Institutions and Nonprofits only

  33. pTXB1-xlH2B(1-114)_K114A

    Plasmid
    #118217
    Purpose
    Bacterial expression plasmid encoding X. laevis H2B residues 1-114(K114A) as a chimeric fusion to GyrA intein with chitin binding domain at the C-terminus. H2B fragment can be derivatized using MESNa.
    Depositor
    Cynthia Wolberger
    Article
    Morgan et al Science. 2016 Feb 12;351(6274):725-8. do
    Insert
    xlH2B(1-114)
    Use
    Tags
    Mxe-GyrA intein fused to chitin binding domain
    Expression
    Bacterial
    Mutation
    Lysine 114 to alanine
    Promoter
    T7
    Available since
    Oct. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    118217-plasmid-map-sequence-id-226718

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  37. pCAGGS-Twin-strep-TEV-DGKδ2-DSAMD

    Plasmid
    #223721
    Purpose
    Express human diacylglycerol kinase delta 2- SAM domain deletion mutant (N-terminal TEV protease cleavable Twin-Strep-fusion protein) in mammalian cell
    Depositor
    Chiaki Murakami
    Insert
    DGKD (DGKD Human)
    Use
    Tags
    TEV cleavable site and Twin-Strep-tag
    Expression
    Mammalian
    Mutation
    deleted sterile alpha motif domain (deleted amino…
    Promoter
    CAG and chicken β-actin promoter
    Available since
    Jan. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    223721-plasmid-map-sequence-id-457432

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  38. pCW57-CMV-MAAP-SL01V6

    Plasmid
    #218249
    Purpose
    1) Ectopic expression of MAAP protein variant in mammalian cells. 2) Packaging MAAP variant sequence into lentivirus for stable expression in mammalian cells.
    Depositor
    David Schaffer
    Article
    Schieferecke et al Mol Ther. 2024 Feb 7;32(2):340-351. doi:
    Insert
    Membrane-Associated Accessory Protein Variant SL01V6 driven by CMV promoter
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    CMV
    Available since
    Oct. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    218249-plasmid-map-sequence-id-449746

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  39. pCW57-CMV-MAAP-SL01V9

    Plasmid
    #218250
    Purpose
    1) Ectopic expression of MAAP protein variant in mammalian cells. 2) Packaging MAAP variant sequence into lentivirus for stable expression in mammalian cells.
    Depositor
    David Schaffer
    Article
    Schieferecke et al Mol Ther. 2024 Feb 7;32(2):340-351. doi:
    Insert
    Membrane-Associated Accessory Protein Variant SL01V9 driven by CMV promoter
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    CMV
    Available since
    Oct. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    218250-plasmid-map-sequence-id-449681

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  40. pCW57-CMV-MAAP-SL08V9

    Plasmid
    #218251
    Purpose
    1) Ectopic expression of MAAP protein variant in mammalian cells. 2) Packaging MAAP variant sequence into lentivirus for stable expression in mammalian cells.
    Depositor
    David Schaffer
    Article
    Schieferecke et al Mol Ther. 2024 Feb 7;32(2):340-351. doi:
    Insert
    Membrane-Associated Accessory Protein Variant SL08V9 driven by CMV promoter
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    CMV
    Available since
    Oct. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    218251-plasmid-map-sequence-id-449683

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  41. pKC31

    Plasmid
    #226777
    Purpose
    Expresses Drosophila UNC-104(R562H) in insect cells
    Depositor
    Kyoko Chiba
    Article
    Niwa et al J Cell Sci. 2024 Sep 6:jcs.262017. doi:
    Insert
    Drosophila unc-104 (R562H) (unc-104 Fly)
    Use
    Tags
    sfGFP and 2xStrepII
    Expression
    Insect
    Mutation
    changed Arginine 562 to Histidine (bris mutation)
    Promoter
    Available since
    Oct. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    226777-plasmid-map-sequence-id-449872

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  42. pCW57-CMV-MAAP-SL01

    Plasmid
    #218248
    Purpose
    1) Ectopic expression of MAAP protein variant in mammalian cells. 2) Packaging MAAP variant sequence into lentivirus for stable expression in mammalian cells.
    Depositor
    David Schaffer
    Article
    Schieferecke et al Mol Ther. 2024 Feb 7;32(2):340-351. doi:
    Insert
    Membrane-Associated Accessory Protein Variant SL01 driven by CMV promoter
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    CMV
    Available since
    Oct. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    218248-plasmid-map-sequence-id-449801

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  48. pGWB602-OEP7-mVenus

    Plasmid
    #213864
    Purpose
    Binary vector for the expression of mVenus flanked with an N-terminal 50 amino acids of chloroplas outer envelope membrane transit peptide in plants.
    Depositor
    Yutaka Kodama
    Article
    Ichikawa et al Plant Direct. 2022 Nov 15;6(11):e462. do
    Insert
    chloroplast outer envelope membrane transit peptide fused mVenus (OEP7 Mustard Weed)
    Use
    Tags
    Expression
    Bacterial and Plant
    Mutation
    Promoter
    Available since
    April 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    213864-plasmid-map-sequence-id-425332

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  50. pcDNA3.1-VcnTS-E1015A

    Plasmid
    #213415
    Purpose
    Vinculin tension sensor (VcnTS) with a single directionally asymmetric, force strengthening (DAFS) variant point mutation (E1015A).
    Depositor
    Brenton Hoffman
    Article
    Chirasani et al Nat Commun. 2023 Dec 14;14(1):8300. doi:
    Insert
    VcnTS-E1015A (VCL Synthetic, Chicken)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Mutated vinculin glutamic acid 1015 to alanine (E…
    Promoter
    CMV
    Available since
    Feb. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    213415-plasmid-map-sequence-id-422214

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Showing: 4901 - 4950 of 23543 results