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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 18,726 results for: IRE

Showing: 4901 - 4950 of 18726 results

  4. pSPORT1[attB/Ins1/NbT promoter/glGFP/bglobin 3'-UTR/Ins2]

    Plasmid
    #74102
    Purpose
    plasmid driven under neuronal beta-tubulin promoter, bearing attB and two insulator sequences (opposite directions pointing inward towards the reporter gene)
    Depositor
    Ben Szaro
    Article
    Wang et al Dev Biol. 2015 Feb 1;398(1):11-23. doi:
    Inserts
    Xenopus laevis beta-tubulin gene promoter region and 5'UTR
    Green Lantern Green Fluorescent Protein
    Rabbit beta 1-globin gene 3'UTR
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    74102-plasmid-map-sequence-id-148113

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  6. pENT-L1-Kozak-Ensconsin-3XGFP-stop-L2

    Plasmid
    #186361
    Purpose
    Entry clone with ORF encoding the MT-binding domain of Ensconsin fused to 3 copies of GFP in C-terminal, flanked by Gateway recombination sequences.
    Depositor
    Patrick Lemaire
    Article
    Roure et al PLoS One. 2007. 2(9):e916.
    Insert
    Human Ensconsin (MAP7 Human, Synthetic)
    Use
    Expression of a fluorescent microtubule marker
    Tags
    3xEGFP
    Expression
    Mutation
    Promoter
    Available since
    Nov. 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    186361-plasmid-map-sequence-id-373431

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  7. pENTR1A mKate2-hu_ncOGT 5N5A

    Plasmid
    #154285
    Purpose
    Entry vector encoding mKate2-2xFLAG-OGT (O-GlcNAc Transferase; 5N5A variant, no HCF-1 cleavage)
    Depositor
    Suzanne Walker
    Article
    Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11
    Insert
    O-GlcNAc Transferase (OGT Human)
    Use
    Gateway entry vector
    Tags
    2x FLAG and mKate2
    Expression
    Mutation
    N322A, N356A, N390A, N424A, N458A mutations (HCF-…
    Promoter
    Available since
    Nov. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    154285-plasmid-map-sequence-id-304861

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  21. pRRL-VcnTS-E1015A-E1021A

    Plasmid
    #213411
    Purpose
    Vinculin tension sensor (VcnTS) with both directionally asymmetric, force strengthening (DAFS) variant point mutations (E1015A and E1021A), in lentiviral expression vector.
    Depositor
    Brenton Hoffman
    Article
    Chirasani et al Nat Commun. 2023 Dec 14;14(1):8300. doi:
    Insert
    VcnTS-E1015A-E1021A (VCL Synthetic, Chicken)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Mutated vinculin glutamic acid 1015 and 1021 to a…
    Promoter
    CMV
    Available since
    Feb. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    213411-plasmid-map-sequence-id-420344

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  22. pUC19-π-EB1_EF1α-mCherry

    Plasmid
    #197429
    Purpose
    Homology-directed repair template targeting EF1α-based π-element to MAPRE1 exon 5
    Depositor
    Torsten Wittmann
    Article
    Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75
    Insert
    EF1α-based π-element with HDR templates flanking MAPRE1 exon 5 insertion site (MAPRE1 Human)
    Use
    CRISPR and Synthetic Biology
    Tags
    GCN4 leucine zipper, LOV2, Zdk1, and mCherry
    Expression
    Mutation
    Promoter
    Available since
    June 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    197429-plasmid-map-sequence-id-388078

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  23. pUC19-π-EB1_EF1α-EGFP

    Plasmid
    #197430
    Purpose
    Homology-directed repair template targeting EF1α-based π-element to MAPRE1 exon 5
    Depositor
    Torsten Wittmann
    Article
    Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75
    Insert
    EF1α-based π-element with HDR templates flanking MAPRE1 exon 5 insertion site (MAPRE1 Human)
    Use
    CRISPR and Synthetic Biology
    Tags
    EGFP, GCN4 leucine zipper, LOV2, and Zdk1
    Expression
    Mutation
    Promoter
    Available since
    June 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    197430-plasmid-map-sequence-id-388175

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  24. ATX3-JD

    Plasmid
    #184247
    Purpose
    Recombinant protein expression and purification. Encodes ataxin-3 Josephin domain fused with His-Tag (N-terminal)
    Depositor
    Sandra Macedo Ribeiro
    Article
    Figueiredo et al Cells. 2022 Jun 19;11(12). pii: cells111
    Insert
    Ataxin-3 Josephin domain (ATXN3 Human)
    Use
    Tags
    6x His-Tag and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Premature stop codon V183X
    Promoter
    T7 promoter
    Available since
    Sept. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    184247-plasmid-map-sequence-id-370313

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Showing: 4901 - 4950 of 18726 results