We narrowed to 15,785 results for: LEA
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pCL1324
Plasmid#217965PurposeBacterial expression of Importin-5 tagged on its N-terminus with GST. Cleavable with 3C protease.DepositorInsertImp5 (IPO5 Human)
UseTagsGSTExpressionBacterialMutationPromoterplacAvailable sinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDN34
Plasmid#61343PurposemCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS2/PKIt NES)DepositorInsertmCherry
UseTagsGS linker-AsLov2-biNLS2 and PKIt NESExpressionMammalianMutationPromoterCMVAvailable sinceMay 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
WN10151
Plasmid#80441PurposeExpresses human codon-optimized dead (832A) LbCpf1DepositorInsertdead (D832A) LbCpf1
UseCRISPRTagsNLS-3xHAExpressionMammalianMutationD832APromoterAvailable sinceAug. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPSup_35Senh(fwd)_35Spr+synJ
Plasmid#149422PurposePlasmid for STARR-seq in tobacco leaves. The GFP reporter gene is under the control of a 35S minimal promoter and an upstream 35S core enhancer.DepositorInsert35S core enhancer
UseTagsExpressionPlantMutationPromoterAvailable sinceAug. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-mCherry-CyrCR1
Plasmid#128768PurposeExpresses mCherry in mammalian cells. ~200 nt of Cyrano inserted in 3'UTR with miR-7 site replaced by miR-17 site.DepositorInsertCyrano conserved region (CR1) with intact miR-7 site (OIP5-AS1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
AviTag-SpyTag003-MBP
Plasmid#184227PurposeExpresses AviTag and SpyTag003 linked to maltose binding protein in the bacterial cytoplasm. AviTag enables site-specific biotinylation by BirA. SpyTag003 enables reaction with SpyCatcher003.DepositorInsertAviTag-SpyTag003-MBP
UseTagsAviTag, His6, SpyTag003, and Thrombin cleavage si…ExpressionBacterialMutationPromoterT7Available sinceJune 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pU6-sgRNA EF1Alpha-puro-T2A-BFP.CYRANO
Plasmid#128755PurposeExpresses CYRANO gRNA for CRISPRiDepositorInsertCYRANO sgRNA (OIP5-AS1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromotermU6Available sinceAug. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pMM0646
Plasmid#129007PurposeIntegration vector for optogenetic machinery (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) and pZF-mRUBY2 with loxable KIURA3 markerDepositorInsertKanR-ColE1 URA 5'-pTEF1-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-pZF(BS)-mRUBY2-tADH1-loxP-KIURA3-loxP-URA3 3'
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mCherry::LANS4
Plasmid#60785PurposeControl of Protein Activity and Cell Fate Specification via Light-Mediated Nuclear TranslocationDepositorInsertLANS4
UseTagsmCherryExpressionBacterial, Insect, and Mamm…MutationPromoterCMVAvailable sinceAug. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
NET29A.Hs pEGFP-N2 (1181)
Plasmid#62040Purposemammalian expression of nuclear envelope transmembrane proteinDepositorInsertNET29A (TMEM120A Human)
UseTagsEGFPExpressionMammalianMutationSplice variantPromoterCMVAvailable sinceJan. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPAP_BGL1
Plasmid#183735PurposeExpresses Aspergillus aculeatus β-glucosidase (BGL1)DepositorInsertβ-glucosidase (AaBGL1)
UseTagsExpressionYeastMutationPromoterAOX1 promoterAvailable sinceFeb. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mCherry-Rheb(5A)
Plasmid#192439PurposeEncodes mCherry-tagged Rheb lacking residues 38-42.DepositorInsertmCherry-Rheb(C181S) (RHEB Human)
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationRheb amino acids 38-42 deleted.PromoterCMVAvailable sinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-TRAIL-R1
Plasmid#61382PurposeExpresses HA tagged TRAIL receptor 1DepositorInsertTRAIL receptor 1
UseTagsHA and TNF receptor leader peptideExpressionMammalianMutationTRAIL-R1 leader peptide (aa1-23) replaced with TN…PromoterAvailable sinceJan. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCVL.SFFV.Kozak.HA.NLS.Y2AniK227M.IRES.BFP
Plasmid#45579PurposeExpresses K227M nickase variant of I-Ani I nuclease in mammalian cells with BFP trackerDepositorInsertcoY2 Ani K227M
UseLentiviralTagsHA, IRES BFP, and NLSExpressionMammalianMutationco indicates codon optimization for mammalian exp…PromoterSFFVAvailable sinceNov. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCAG-nucGFP11-3xControlgRNA
Plasmid#224568PurposeUbiquitous expression plasmid, CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), three Control gRNAs with ribozyme self-cleavage sites, and nuclear split GFP(11) reporter.DepositorInsertGFP11-H2B
UseCRISPRTagsHistone H2B (nuclear localization)ExpressionMutationCodon optimizedPromoterAvailable sinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-mCherry-CyrCR1.miR16
Plasmid#128747PurposeExpresses mCherry in mammalian cells. ~200 nt of Cyrano with miR-7 site replaced by miR-16 site inserted in mCherry 3'UTR.DepositorInsertCyrano conserved region (CR1) with miR-16 site replacing miR-7 site (OIP5-AS1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDB22
Plasmid#61342PurposeMammalian expression of mCherry fused to LINuS, a light-inducible nuclear localization signal (cMycP1A NLS/PKIt NES)DepositorInsertmCherry
UseTagsGS linker-AsLov2-cMyc1A NLS and PKIt NESExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTetOne-DsRed-cNLS
Plasmid#138528PurposeInducible expression of DsRed-Express2 with nuclear localization signalDepositorInsertDsRed-Express2
UseTags3x SV40 cNLSExpressionMammalianMutationPromoterTRE3GsAvailable sinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only
