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Plasmid#109874PurposeProtein expression and purification of SUB1_PC4DepositorInsertSUB1_PC4 (SUB1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 PARL-FLAG-CT L79E
Plasmid#13640DepositorUseTagsFLAGExpressionMammalianMutationchanged Leu 79 to GluPromoterAvailable sinceJan. 4, 2007AvailabilityAcademic Institutions and Nonprofits only -
pJZ001
Plasmid#120230PurposeContains intB13 integrase casette for knock-in to pCAP-BAC backboneDepositorInsertintB13 integrase casette
UseTagsExpressionBacterialMutationbla-g717a (amp resistance gene)PromoterAvailable sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pXylA-agrCA-IV
Plasmid#53438Purposexylose-dependent expression of the type-IV agrC and type-I agrA genes of S. aureusDepositorInsertagrCA locus
UseSynthetic BiologyTagsnoneExpressionBacterialMutation"MVQTS" sequence added to the N-terminu…PromoterPxylAAvailable sinceJune 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_UBA52_Ubiquitin
Plasmid#110043PurposeProtein expression and purification of UBA52_UbiquitinDepositorInsertUBA52_Ubiquitin (UBA52 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_SPAG1_TPR
Plasmid#109873PurposeProtein expression and purification of SPAG1_TPRDepositorInsertSPAG1_TPR (SPAG1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_U2AF1_RRM
Plasmid#110033PurposeProtein expression and purification of U2AF1_RRMDepositorInsertU2AF1_RRM (U2AF1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_NXT1_NTF2
Plasmid#109803PurposeProtein expression and purification of NXT1_NTF2DepositorInsertNXT1_NTF2 (NXT1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDA125
Plasmid#74249PurposeIntermediate cloning vector, with MCS2 containing inducible unstable peptide driven by pSTL1 (UbiY 2xNLSs SZ1).DepositorInsertInducible peptide of the dPSTR
UseTagsExpressionBacterial and YeastMutationPromoterpSTL1Available sinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_VCL_Vinculin
Plasmid#110045PurposeProtein expression and purification of VCL_VinculinDepositorInsertVCL_Vinculin (VCL Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFP.R365
Plasmid#138235PurposeExpresses the split mCherry reporter interrupted by the EP-Pri artificially mini-intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialMutationPromoterP(araBAD) and iPCAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-CBX1wt-PA-mCherry
Plasmid#138252PurposeWild-type CBX1 chromodomain (residues 20-73) tagged with photoactivatable mCherry (PAmCherry).DepositorInsertCBX1-PAmCherry
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_TOM1L2_VHS
Plasmid#109901PurposeProtein expression and purification of TOM1L2_VHSDepositorInsertTOM1L2_VHS (TOM1L2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZF-ADD-L
Plasmid#127477Purposecoding sequence for synthetic zinc finger ZF-ADD-LDepositorInsertZF-ADD synthetic transcription factor fused with PDZ cognate peptide ligand (VKESLV)
UseCell-free transcription–translationTagsPDZ cognate peptide ligand (VKESLV)ExpressionMutationPromoterT7Available sinceNov. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_PPARD_Hormone-recep
Plasmid#109955PurposeProtein expression and purification of PPARD_Hormone-recepDepositorInsertPPARD_Hormone-recep
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28-his-3C_Clu3B6p
Plasmid#100093Purposeexpresses easily crystallizable engineered 14-3-3 sigma variant fused to the HSPB6 phosphorylatable peptideDepositorInsert14-3-3 sigma human (SFN Human)
UseTagsHSPB6 peptide and His6ExpressionBacterialMutation75-77 residues changed to AAA to facilitate cryst…PromoterT7Available sinceSept. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSO7
Plasmid#106365PurposeTo insert a single copy of a construct at a site on Chr I, corresponding to the Shigella flexneri protein OspF (a MAP kinase inhibitor) expressed specifically in the epidermis.DepositorInsertttTi4348_ SEC _pcol-19_OspF_3'unc54UTR
UseTagsExpressionWormMutationPromoterAvailable sinceJuly 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPROEX Hsp104 (773-908)
Plasmid#1316DepositorInsertHsp104 (773-908) (HSP104 Budding Yeast)
UseTags6X His + TEV cleavageExpressionBacterialMutationdeletion of aa1-773PromoterAvailable sinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pPROEX Hsp104 (361-548)
Plasmid#1317DepositorInsertHsp104 (361-548) (HSP104 Budding Yeast)
UseTags6X His + TEV cleavageExpressionBacterialMutationaa361-548 of Hsp104PromoterAvailable sinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
mNG-mCh
Plasmid#124215PurposeCytoplasmic mNeongreen mCherry tandem, ~19 % energy transfer pNM002 - pSAV057-mNG-mChDepositorInsertmNG-mCh
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 23, 2019AvailabilityAcademic Institutions and Nonprofits only