We narrowed to 5,314 results for: phy

Showing: 1961 - 1980 of 5314 results

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pFRETgc-2in1-CN

Plasmid

#105118

Purpose

FRET/FLIM analysis in plants (stable or transient transformation)

Depositor

Christopher Grefen

Article

Hecker et al Plant Physiol. 2015 Jul;168(3):776-87. d

Type

Empty backbone

Tags

mCherry and mEGFP

Expression

Plant

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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IFP1.4-N1

Plasmid

#54783

Purpose

Localization: N1 Cloning Vector, Excitation: 684, Emission: 708

Depositor

Michael Davidson, Roger Tsien

Article

Shu et al Science. 2009 May 8;324(5928):804-7. doi

Type

Empty backbone

Tags

IFP1.4

Expression

Mammalian

Promoter

CMV

Available Since

June 20, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pYPQ155

Plasmid

#69306

Purpose

Gateway entry vector with AteCas9D10A

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Insert

AteCas9D10A (Arabidopsis codon optimized)

Use

CRISPR

Mutation

D10A mutation; NLS fusion

Available Since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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hSDH5_C83D

Plasmid

#113048

Purpose

Expression of mutant hSDH5

Depositor

Edward A. Berry

Article

Zafreen et al Arch Biochem Biophys. 2016 Aug 15;604:47

Insert

hSDH5_C83D (SDHAF2 Human)

Expression

Bacterial

Mutation

C83D, Insert begins at amino acid 39

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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mEos2-Tubulin-C-18

Plasmid

#57432

Purpose

Localization: Microtubules, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

Tubulin (TUBA1B Human)

Tags

mEos2

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pENTR-PpU6P-sgRNA-L5L2

Plasmid

#113738

Purpose

Gateway entry vector containing attL5 and attL2 sites, U6 promoter, BsaI sites for protospacer ligation, and sgRNA.

Depositor

Magdalena Bezanilla

Article

Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

Insert

PpU6P::sgRNA

Use

CRISPR and Gateway Cloning

Promoter

P. patens U6 promoter

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR-PpU6P-sgRNA-R4R3

Plasmid

#113739

Purpose

Gateway entry vector containing attR4 and attR3 sites, U6 promoter, BsaI sites for protospacer ligation, and sgRNA.

Depositor

Magdalena Bezanilla

Article

Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

Insert

PpU6P::sgRNA

Use

CRISPR and Gateway Cloning

Promoter

P. patens U6 promoter

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR-PpU6P-sgRNA-L5L4

Plasmid

#113741

Purpose

Gateway entry vector containing attL5 and attL4 sites, U6 promoter, BsaI sites for protospacer ligation, and sgRNA.

Depositor

Magdalena Bezanilla

Article

Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

Insert

PpU6P::sgRNA

Use

CRISPR and Gateway Cloning

Promoter

P. patens U6 promoter

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR-PpU6P-sgRNA-L1R5

Plasmid

#113737

Purpose

Gateway entry vector containing attL1 and attR5 sites, U6 promoter, BsaI sites for protospacer ligation, and sgRNA.

Depositor

Magdalena Bezanilla

Article

Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

Insert

PpU6P::sgRNA

Use

CRISPR and Gateway Cloning

Promoter

P. patens U6 promoter

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-160

Plasmid

#79840

Purpose

Expresses NLS-PpsR2-mVenus under CMV promoter

Depositor

Vladislav Verkhusha

Article

Kaberniuk et al Nat Methods. 2016 May 9. doi: 10.1038/nm

Insert

NLS-PpsR2-mVenus

Expression

Mammalian

Mutation

RpPpsR2 cysteine 439 changed to serine

Promoter

CMV

Available Since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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pMOS017E: Entry vector for: ratiometric pHluorin (cytosolic)

Plasmid

#163069

Purpose

Entry vector for: ratiometric pHluorin (cytosolic)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

ratiometric pHluorin

Use

Gateway Cloning

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pCfB8381

Plasmid

#126915

Purpose

Plasmid containing integration cassette at XI-3 integration site in Saccharomyces cerevisiae for overexpression of XdCrtI and tHMG1

Depositor

Irina Borodina

Article

Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

Insert

XdCrtI and tHMG1

Expression

Yeast

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR-TIR1

Plasmid

#83875

Purpose

Gateway donor vector for TIR1

Depositor

Jennifer Nemhauser

Article

Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

Insert

TIR1 (TIR1 Mustard Weed)

Use

Gateway Cloning

Available Since

Oct. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pRSETA_crE6G2

Plasmid

#172929

Purpose

Crowding FRET sensor with mCerulean3 and mCitrine for E. coli expression. Linker comprises 2 helices, and a 6AA GSG domain

Depositor

Arnold Boersma

Article

Liu et al Biophys J. 2017 May 9;112(9):1929-1939.

Insert

CrowdingSensorE6G2

Tags

His

Expression

Bacterial

Mutation

Val317Gly, Met316Gly

Promoter

T7

Available Since

Aug. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pRVdG-mCherry-N-P-M-EGFP-SynPhRFP-L

Plasmid

#52481

Purpose

Expresses mCherry, EGFP and Synaptophysin-TagRFP-T

Depositor

Sebastian Seung

Article

Wickersham et al Nat Commun. 2013;4:2332. doi: 10.1038/nc

Inserts

mCherry
EGFP
Synaptophysin-TagRFP-T

Use

Rabies virus

Tags

TagRFP-T

Expression

Mammalian

Promoter

CMV

Available Since

May 23, 2014

Availability

Academic Institutions and Nonprofits only

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pEPOR0SP0020

Plasmid

#117531

Purpose

Level0 Golden Gate part: CDS, SaCas9

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

SaCas9 (with stop codon)

Use

CRISPR

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pCS2 xRor2-mCherry

Plasmid

#67162

Purpose

mammalian expression / in vitro transcription

Depositor

Alexandra Schambony

Article

Feike et al Biochem Biophys Res Commun. 2010 Oct 1;4

Insert

Tyrosine-protein kinase transmembrane receptor ROR2 (ror2 Frog)

Use

In vitro transcription

Tags

mCherry

Expression

Mammalian

Available Since

July 16, 2015

Availability

Academic Institutions and Nonprofits only
pQIq_MRGS_HIS8_(27G2)_FLAG

Plasmid

#199616

Purpose

Encodes DARPin-FLAG 27G2 for bacterial expression and column purification

Depositor

Shiva Tyagarajan

Article

Campbell et al Elife. 2022 Oct 31;11:e80895. doi: 10.75

Insert

DARPin-FLAG 27G2

Tags

8xHis and FLAG

Expression

Bacterial

Promoter

T5

Available Since

May 1, 2023

Availability

Academic Institutions and Nonprofits only

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Keratin 18 R89C

Plasmid

#18059

Depositor

M Bishr Omary

Article

Ku et al J Cell Biol. 1994 Oct . 127(1):161-71.

Insert

Keratin 18 (KRT18 Human)

Expression

Mammalian

Mutation

R89C

Available Since

May 16, 2008

Availability

Academic Institutions and Nonprofits only
pMOS011E: Entry vector for: superecliptic pHluorin (mitochondrial)

Plasmid

#163067

Purpose

Entry vector for: superecliptic pHluorin (mitochondrial)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

superecliptic pHluorin

Use

Gateway Cloning

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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