We narrowed to 29,080 results for: ple

1. plenti-CAG-gate-FLAG-IRES-GFP

   Plasmid

   #107398

   Purpose

   Lentiviral gateway destination vector

   Depositor

   William Kaelin

   Article

   Lu et al Science. 2014 Jan 17;343(6168):305-9. do

   Type

   Empty backbone

   Use

   Lentiviral; Gateway destination

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. plentiCMV10x-sct-pp65-b2m-HLA-A0201

   Plasmid

   #196513

   Purpose

   Express single-chain pMHC with sequencing tag for 10x genomics

   Depositor

   Howard Chang

   Article

   Yu et al Cell. 2022 Dec 22;185(26):4904-4920.e22.

   Insert

   single chain of a pp65 peptide, b2m and HLA A0201
   

   Use

   Lentiviral

   Tags

   NA

   Expression

   Mammalian

   Mutation

   Y84C, 10xgenomics compatible tag

   Promoter

   CMV

   Available since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti PGK FKBP12(F36V)-OGT (Neo)

   Plasmid

   #154294

   Purpose

   Lentiviral vector encoding FKBP12(F36V)-2xHA-OGT (Neomycin resistant) off PGK promoter

   Depositor

   Suzanne Walker

   Article

   Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

   Insert

   O-GlcNAc Transferase (OGT Human)
   

   Use

   Lentiviral

   Tags

   2x HA and FKBP12(F36V)

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-dCas9-KRAB-gRNA-TRE-blast

   Plasmid

   #201152

   Purpose

   Lentiviral expression of S. pyogenes dead Cas9 (dCas9/dSpCas9/SpdCas9) fused to the KRAB domain as well as a gRNA targeting the tight TRE promoter and the blasticidin resistance gene.

   Depositor

   Moshe Szyf

   Inserts

   dCas9-KRAB
   gRNA-TRE
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mutation

   gRNA sequence: TACGTTCTCTATCACTGAT

   Promoter

   Available since

   July 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. plentiCMV-sct-pp65-b2m-HLA-A0201

   Plasmid

   #196511

   Purpose

   Express single-chain peptide-MHC (pp65 CMV epitope on HLA-A0201)

   Depositor

   Howard Chang

   Article

   Yu et al Cell. 2022 Dec 22;185(26):4904-4920.e22.

   Insert

   single chain of a pp65 peptide, b2m and HLA A0201
   

   Use

   Lentiviral

   Tags

   NA

   Expression

   Mammalian

   Mutation

   Y84C

   Promoter

   CMV

   Available since

   March 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti-DHB-mVenus-p2a-mCherry-CDK4KTR

   Plasmid

   #126679

   Purpose

   Fluorescent reporter for cyclin E/A-CDK and CDK4/6 activities

   Depositor

   Hee Won Yang

   Article

   Yang et al Elife. 2020 Apr 7;9. pii: 44571. doi: 10

   Insert

   Rb (a.a 886-928)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-Synapsin-hChR2(H134R)-EYFP-WPRE

   Plasmid

   #20945

   Purpose

   3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to EYFP driven by human Synapsin I promoter for optogenetic activation

   Depositor

   Karl Deisseroth

   Article

   Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

   Insert

   channelrhodopsin-2
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   H134R mutation in ChR2

   Promoter

   hSyn

   Available since

   May 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti-FNLS-P2A-GFP-PGK-Puro

   Plasmid

   #110869

   Purpose

   Lentiviral vector for constitutive expression of FNLS-P2A-GFP in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   BE3RA-FNLS
   

   Use

   Lentiviral

   Tags

   3X FLAG

   Expression

   Mutation

   D10A and NLS sequence at the N-terminus

   Promoter

   EF1s

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti-CMV-GSDMD-eGFP-SV40p-BlasR

   Plasmid

   #228254

   Purpose

   pLenti-CMV-GSDMD-eGFP-SV40p-BlasR plasmid for ectopic expression of human full-length GSDMD fusion with GFP

   Depositor

   Judy Lieberman

   Article

   Fontana et al Cell. 2024 Sep 2:S0092-8674(24)00898-5.

   Insert

   GSDMD (GSDMD Human)
   

   Use

   Lentiviral

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti-VQR-P2A-GFP-PGK-Puro

    Plasmid

    #110861

    Purpose

    Lentiviral vector for constitutive expression of Cas9-VQR-P2A-GFP (not codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    Cas9-VQR
    

    Use

    Lentiviral

    Tags

    3X FLAG

    Expression

    Mutation

    D1135V, R1335Q, T1337R

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. plenti-UBC- IKZF4-3xHA-pGK-PUR

    Plasmid

    #107384

    Purpose

    Lentiviral expression of IKZF4-3xHA

    Depositor

    William Kaelin

    Article

    Lu et al Science. 2014 Jan 17;343(6168):305-9. do

    Insert

    IKZF4 (IKZF4 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    UBC

    Available since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti-C-Myc-DDK-IRES-Puro METTL3

    Plasmid

    #200724

    Purpose

    Lentiviral expression of Flag-Tagged human METTL3

    Depositor

    Claudio Alarcón

    Insert

    METTL3 (METTL3 Human)
    

    Use

    Lentiviral

    Tags

    Myc-DDK from backbone plasmid

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. plenti-CAG-IKZF1-V1-FLAG-IRES-GFP

    Plasmid

    #107387

    Purpose

    Lentiviral expression of IKZF1-V1-FLAG

    Depositor

    William Kaelin

    Article

    Lu et al Science. 2014 Jan 17;343(6168):305-9. do

    Insert

    IKZF1-V1 (IKZF1 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSimpleII-U6-tracr-U6-BsmBI-NLS-NmCas9-HA-NLS(s)

    Plasmid

    #47868

    Purpose

    This plasmid contains expression cassette for NmCas9 with N and C NLS and an HA tag, a cassette for expression of tracrRNA, a cassette for cloning crRNA under the control of U6 promoter.

    Depositor

    James Thomson, Erik Sontheimer

    Article

    Hou et al Proc Natl Acad Sci U S A. 2013 Aug 12.

    Inserts

    NmCas9
    U6pr-tracrRNA
    

    Use

    CRISPR

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a and U6

    Available since

    Sept. 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti-CaMKIIa-hChR2(C128S)-EYFP-WPRE

    Plasmid

    #20294

    Purpose

    Lentiviral expression of ChR2 (C128S) Step Function Opsin (SFO) fused to EYFP, for bi-stable optogenetic excitation

    Depositor

    Karl Deisseroth

    Article

    Berndt et al Nat Neurosci. 2009 Feb . 12(2):229-34.

    Insert

    channelrhodopsin-2
    

    Use

    Lentiviral

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    changed Cysteine 128 to Serine

    Promoter

    Available since

    May 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti HRE mutant-Luc pGK Hygro

    Plasmid

    #118707

    Purpose

    reporter plasmid, HIF-responsive promoter (3xHRE) mutant- fused to firefly luciferase

    Depositor

    William Kaelin

    Article

    Briggs et al Cell. 2016 Jun 30;166(1):126-39. doi: 10

    Insert

    HREmutant-Luc
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti NLuc398-G8NCRD IRES G8NCRD-CLuc394

    Plasmid

    #128386

    Purpose

    Expresses a split firefly luciferase reporter based on the N terminal carbohydrate recognition domain of human galectin 8, which concentrates inside endosomes following endosomal disruption

    Depositor

    Craig Duvall

    Article

    Kilchrist et al ACS Sens. 2020 Jun 23. doi: 10.1021/acss

    Inserts

    NLuc-3xGGGGS-Gal8-NCRD (LGALS8 Synthetic, Human)
    Gal8-NCRD-3xGGGGS-CLuc398 (LGALS8 Human)
    

    Use

    Lentiviral, Luciferase, and Synthetic Biology

    Tags

    C-terminal luc2 fragment and N-terminal luc2 frag…

    Expression

    Mammalian

    Mutation

    N terminal carbohydrate recognition domain fused …

    Promoter

    EF1A and IRES

    Available since

    Jan. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti-CMV-eGFP-hTau P301L-WPRE

    Plasmid

    #233043

    Purpose

    To Express a GFP human Tau P301L fusion protein from a CMV promoter

    Depositor

    Jonathan Ploski

    Insert

    eGFP-hTau P301L fusion (MAPT Synthetic, Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    Available since

    March 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti-C-Myc-DDK-IRES-Puro HEXIM1

    Plasmid

    #200726

    Purpose

    Lentiviral expression of Flag-Tagged human HEXIM1

    Depositor

    Claudio Alarcón

    Insert

    HEXIM1 (HEXIM1 Human)
    

    Use

    Lentiviral

    Tags

    Myc-DDK from backbone plasmid

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLenti-CaMKIIa-hChR2(H134R)-mCherry-WPRE

    Plasmid

    #20943

    Purpose

    3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to mCherry driven by CaMKIIa promoter for optogenetic activation

    Depositor

    Karl Deisseroth

    Article

    Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

    Insert

    channelrhodopsin-2
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    H134R

    Promoter

    CaMKIIa

    Available since

    July 9, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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