Addgene

We narrowed to 314 results for: SaCas9

Showing: 21 - 40 of 314 results

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dSaCas9-KRAB

Plasmid

#205250

Purpose

In vitro transcription plasmid for production of dSaCas9-KOX1 KRAB mRNA for transcriptional activation (KRAB = Krüppel-associated box). Insert is derived from Addgene plasmid #106219.

Depositor

Rasmus Bak

Insert

dSaCas9-KRAB

Use

CRISPR

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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dSaCas9-VPR

Plasmid

#205251

Purpose

In vitro transcription plasmid for production of dSaCas9-VPR mRNA for transcriptional activation (VPR = tripartite transactivator VP64, p65, and Rta). Insert is derived from Addgene plasmid #68495

Depositor

Rasmus Bak

Article

Jensen et al Genome Res. 2021 Nov;31(11):2120-2130. d

Insert

dSaCas9-VPR

Use

CRISPR

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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CAG-SaCas9-WPRE

Plasmid

#89996

Purpose

Expresses high levels of WT SaCas9 for genome editing

Depositor

Timo Otonkoski

Article

Balboa et al Stem Cell Res. 2017 Jul;22:16-19. doi: 1

Insert

SaCas9 (NEWENTRY Staphylococcus aureus)

Use

Tags

3xHA, Nucleoplasmin NLS, and SV40 NLS

Expression

Mammalian

Mutation

Promoter

CAG

Availability

Academic Institutions and Nonprofits only

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U6-hGRIN2B-CAG-ps-SaCas9

Plasmid

#102853

Purpose

A single-chain light-controllable dSaCas9 with pdDronpa1 domains for hGRIN2B gene editing

Depositor

Michael Lin

Article

Zhou et al ACS Chem Biol. 2017 Sep 29. doi: 10.1021

Insert

sa-hGRIN2B-sgRNA; dSaCas9; pdDronpa1 (GRIN2B S. aureus, Synthetic, Human)

Use

CRISPR

Tags

3X Flag and NLS

Expression

Mammalian

Mutation

Promoter

U6 promoter;

Availability

Academic Institutions and Nonprofits only

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pX551-miniCMV-SaCas9

Plasmid

#107032

Purpose

AAV plasmid expressing SaCas9 under control of miniCMV promoter

Depositor

Alex Hewitt

Insert

SaCas9

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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miniABEMax V82G nSaCas9

Plasmid

#135365

Purpose

Expression of SaCas9 nickase (D10A) with single evolved TadA monomer (ABEMax) with a V82G substitution

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

miniABEMax V82G nSaCas9

Use

CRISPR

Tags

Expression

Mammalian

Mutation

(V82G)

Promoter

Availability

Academic Institutions and Nonprofits only

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AAV_NLS-dSaCas9-NLS-VPR

Plasmid

#68495

Purpose

AAV vector containing nuclease null SaCas9 fused to VPR

Depositor

George Church

Article

Kiani et al Nat Methods. 2015 Sep 7. doi: 10.1038/nm

Insert

dSaCas9

Use

AAV

Tags

VPR

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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AAV_NLS-SaCas9-NLS-VPR

Plasmid

#68496

Purpose

AAV vector containing SaCas9 fused to VPR

Depositor

George Church

Article

Kiani et al Nat Methods. 2015 Sep 7. doi: 10.1038/nm

Insert

SaCas9-VPR

Use

AAV and CRISPR

Tags

VPR

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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Icam 2 SaCas9 YAP sgRNA1

Plasmid

#99734

Purpose

Expresses SaCas9 and a gRNA targeting mouse YAP

Depositor

Yu Huang

Article

Wang et al Nature. 2016 Dec 7. doi: 10.1038/nature2

Insert

SaCas9 gRNA targeting Yap1 (Yap1 Mouse)

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Availability

Academic Institutions and Nonprofits only

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Icam 2 SaCas9 YAP sgRNA2

Plasmid

#99735

Purpose

Expresses SaCas9 and a gRNA targeting mouse YAP

Depositor

Yu Huang

Article

Wang et al Nature. 2016 Dec 7. doi: 10.1038/nature2

Insert

SaCas9 gRNA targeting Yap1 (Yap1 Mouse)

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Availability

Academic Institutions and Nonprofits only

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Icam 2 SaCas9 YAP sgRNA3

Plasmid

#99736

Purpose

Expresses SaCas9 and a gRNA targeting mouse YAP

Depositor

Yu Huang

Article

Wang et al Nature. 2016 Dec 7. doi: 10.1038/nature2

Insert

SaCas9 gRNA targeting Yap1 (Yap1 Mouse)

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEX-SaCas9-U6-sgRNA

Plasmid

#124844

Purpose

Vector for Cre-dependent expression of SaCas9

Depositor

Larry Zweifel

Article

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

SaCas9, gRNA scaffold

Use

AAV, CRISPR, and Mouse Targeting

Tags

NLS and NLS-3xHA

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEXfrt-SaCas9-U6-sgRNA

Plasmid

#124845

Purpose

Vector for FLP-dependent expression of SaCas9 with gRNA

Depositor

Larry Zweifel

Article

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

SauCas9 gRNA scaffold

Use

AAV, CRISPR, and Mouse Targeting

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEX-SaCas9-U6-sgSlc17a7

Plasmid

#124846

Purpose

Mutagenesis of Slc17a7

Depositor

Larry Zweifel

Article

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

Slc17a7 (Slc17a7 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEX-SaCas9-U6-sgSlc17a6

Plasmid

#124847

Purpose

Mutagenesis of Slc17a6 with SauCas9

Depositor

Larry Zweifel

Article

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

Slc17a6 gRNA (Slc17a6 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEX-SaCas9-U6-sgSlc17a8

Plasmid

#124848

Purpose

Mutagenesis of Slc17a8

Depositor

Larry Zweifel

Article

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

Slc17a8 (Slc17a8 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEX-SaCas9-U6-sgSlc18a2

Plasmid

#124849

Purpose

Mutagenesis of Slc18a2

Depositor

Larry Zweifel

Article

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

Slc18a2 (Slc18a2 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEX-SaCas9-U6-sgGrin2a

Plasmid

#124850

Purpose

Mutagenesis of Grin2a

Depositor

Larry Zweifel

Article

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

Grin2a (Grin2a Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEX-SaCas9-U6-sgGrin2b

Plasmid

#124851

Purpose

Mutagenesis of Grin2b

Depositor

Larry Zweifel

Article

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

Grin2b (Grin2b Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEX-SaCas9-U6-sgGrin1

Plasmid

#124852

Purpose

Mutagenesis of Grin1

Depositor

Larry Zweifel

Article

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

Grin1 (Grin1 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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