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Addgene
Showing: 21 - 40 of 307 results
  1. pBad-CA TAG126

    Plasmid
    #105836
    Purpose
    Expresses thermostable variant of human carbonic anhydrase II with TAG stop codon at site 126 and C-terminal 6x his tag
    Depositor
    Article
    Insert
    human carbonic anhydrase II thermostable variant (CA2 Human)
    Use
    Tags
    6x his tag
    Expression
    Bacterial
    Mutation
    TAG stop codon at site 126
    Promoter
    pBad/arabinose
    Availability
    Academic Institutions and Nonprofits only
  2. pBad-CA TAG186

    Plasmid
    #105837
    Purpose
    Expresses thermostable variant of human carbonic anhydrase II with TAG stop codon at site 186 and C-terminal 6x his tag
    Depositor
    Article
    Insert
    human carbonic anhydrase II thermostable variant (CA2 Human)
    Use
    Tags
    6x his tag
    Expression
    Bacterial
    Mutation
    TAG stop codon at site 186
    Promoter
    pBad/arabinose
    Availability
    Academic Institutions and Nonprofits only
  3. DAG1A-c003

    Plasmid
    #205107
    Purpose
    DAG1 protein expression
    Depositor
    Insert
    DAG1 (DAG1 Human)
    Use
    Tags
    AviTag-6His and PTPRSsp
    Expression
    Mammalian
    Mutation
    WT
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  4. Lenti-DAG1

    Plasmid
    #205149
    Purpose
    Overexpress DAG1
    Depositor
    Insert
    Dystroglycan 1 (DAG1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Carries common missense variant c.41C>G (p.Ser…
    Promoter
    EF-1a
    Availability
    Academic Institutions and Nonprofits only
  5. DAG1-bio-His

    Plasmid
    #51651
    Purpose
    Expresses full-length Dystroglycan precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Insert
    DAG1 (DAG1 Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
  6. pSAG1::CAS9-U6::sgUPRT

    Plasmid
    #54467
    Purpose
    Encodes CAS9 nuclease (GFP fusion) under control of the Toxoplasma gondii SAG1 promotor. Vector also provides U6 controlled expression of a single guide RNA for disruption of genes by CRISPR.
    Depositor
    Insert
    SAG1 5' UTR
    Use
    CRISPR; Toxoplasma expression
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
Showing: 21 - 40 of 307 results