Addgene

We narrowed to 3,944 results for: gast

Showing: 21 - 40 of 3944 results

Results per page:

pUDP013

Plasmid

#103873

Purpose

Broad-host-range Cas9/gRNA co-expression plasmid with gRNA targetting ADE2 from Ogataea (para)polymorpha

Depositor

Jean-Marc Daran

Article

Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4

Insert

HH-gRNA-HDV targetting ADE2 in Ogataea (para)polymorpha

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

ScTDH3

Availability

Academic Institutions and Nonprofits only

Enlarge
pUDP025

Plasmid

#103874

Purpose

Broad-host-range Cas9/gRNA co-expression plasmid with gRNA targetting ADE2 from Kluyveromyces lactis

Depositor

Jean-Marc Daran

Article

Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4

Insert

HH-gRNA-HDV targetting ADE2 in Kluyveromyces lactis

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

ScTDH3

Availability

Academic Institutions and Nonprofits only

Enlarge
pUDP082

Plasmid

#103875

Purpose

Broad-host-range Cas9/gRNA co-expression plasmid with gRNA targetting ADE2 from Kluyveromyces marxianus

Depositor

Jean-Marc Daran

Article

Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4

Insert

HH-gRNA-HDV targetting ADE2 in Kluyveromyces marxianus

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

ScTDH3

Availability

Academic Institutions and Nonprofits only

Enlarge
pUDP123

Plasmid

#107269

Purpose

pUDP123 expressing a polycistronic g-RNA array for Cas9 editing targeting the genes OpADE2 and OpNIAD and Spcas9D147Y P411T in O. parapolymorpha (HH-gRNAOpADE2-HDV-linker-HH-gRNAOpNIAD-HDV)

Depositor

Jean-Marc Daran

Article

Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4

Insert

polycistronic HH-gRNA-HDV-HH-gRNA-HDV array targetting OpADE2 and NIAD in O. parapolymorpha

Use

Tags

Expression

Yeast

Mutation

Promoter

ScTDH3

Availability

Academic Institutions and Nonprofits only

Enlarge
pUDP002

Plasmid

#103872

Purpose

Broad-host-range Cas9/gRNA co-expression backbone plasmid (no gRNA)

Depositor

Jean-Marc Daran

Article

Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4

Inserts

hph
Spcas9 D147Y P411T

Use

CRISPR

Tags

NLS

Expression

Yeast

Mutation

D147Y P411T

Promoter

Arxula adeninivorans TEF1 and Ashbya gossypii (Er…

Availability

Academic Institutions and Nonprofits only

Enlarge
Drosophila_sgRNA_group_1

Pooled Library

#134582

Purpose

Targets kinases, phosphatases, and Drosophila orthologs of drug targets

Depositor

Norbert Perrimon

Article

Viswanatha et al Elife. 2018 Jul 27;7. pii: 36333. doi: 1

Expression

Insect

Species

Drosophila melanogaster

Use

CRISPR

Availability

Academic Institutions and Nonprofits only
Drosophila_sgRNA_group_3

Pooled Library

#134584

Purpose

Targets all other Drosophila genes not included in Groups 1 and 2

Depositor

Norbert Perrimon

Article

Viswanatha et al Elife. 2018 Jul 27;7. pii: 36333. doi: 1

Expression

Insect

Species

Drosophila melanogaster

Use

CRISPR

Availability

Academic Institutions and Nonprofits only
Drosophila_sgRNA_group_2

Pooled Library

#134583

Purpose

Targets Drosophila orthologs of vertebrate genes that have undergone expansion in the vertebrate line

Depositor

Norbert Perrimon

Article

Viswanatha et al Elife. 2018 Jul 27;7. pii: 36333. doi: 1

Expression

Insect

Species

Drosophila melanogaster

Use

CRISPR

Availability

Academic Institutions and Nonprofits only
Fly 2.0 pIDTBlue

Plasmid

#117418

Purpose

Drosophila melanogaster gene cloned into pIDTBlue vector for internal extraction control

Depositor

Dirk Dittmer

Insert

FLY 2.0

Use

Tags

NA

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
AByA

Plasmid

#163749

Purpose

Integrates marker gene onto D. melanogaster Y chromosome

Depositor

Omar Akbari

Article

Buchman et al Insect Mol Biol. 2018 Aug 6. doi: 10.111

Insert

Y-Left-GypSy_3xp3-optimized-tdtomato-Attp-CTCF-Y-Right

Use

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
AByB

Plasmid

#164148

Purpose

Integrates marker gene onto D. melanogaster Y chromosome

Depositor

Omar Akbari

Article

Buchman et al Insect Mol Biol. 2018 Aug 6. doi: 10.111

Insert

Y-Left-GypSy_3xp3-optimized-tdtomato-Attp-CTCF-Y-Right

Use

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
AByC

Plasmid

#164149

Purpose

Integrates marker gene onto D. melanogaster Y chromosome

Depositor

Omar Akbari

Article

Buchman et al Insect Mol Biol. 2018 Aug 6. doi: 10.111

Insert

Y-Left-GypSy_3xp3-optimized-tdtomato-Attp-CTCF-Y-Right

Use

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
AByD

Plasmid

#164150

Purpose

Integrates marker gene onto D. melanogaster Y chromosome

Depositor

Omar Akbari

Article

Buchman et al Insect Mol Biol. 2018 Aug 6. doi: 10.111

Insert

Y-Left-GypSy_3xp3-optimized-tdtomato-Attp-CTCF-Y-Right

Use

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
AByH

Plasmid

#164152

Purpose

Integrates marker gene onto D. melanogaster Y chromosome

Depositor

Omar Akbari

Article

Buchman et al Insect Mol Biol. 2018 Aug 6. doi: 10.111

Insert

Y-Left-GypSy_3xp3-optimized-tdtomato-Attp-CTCF-Y-Right

Use

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
AByI

Plasmid

#164153

Purpose

Integrates marker gene onto D. melanogaster Y chromosome

Depositor

Omar Akbari

Article

Buchman et al Insect Mol Biol. 2018 Aug 6. doi: 10.111

Insert

Y-Left-GypSy_3xp3-optimized-tdtomato-Attp-CTCF-Y-Right

Use

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pIS1

Plasmid

#74887

Purpose

targeting vector for C-terminal eGFP tag on Drosophila melanogaster Act5C

Depositor

Klaus Foerstemann

Article

Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

Inserts

Act5C upstream homology region
Act5C downstream homology

Use

Tags

eGFP

Expression

Insect

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
AByG

Plasmid

#111083

Purpose

Integrates marker gene onto D. melanogaster Y chromosome

Depositor

Omar Akbari

Article

Buchman et al Insect Mol Biol. 2018 Aug 6. doi: 10.111

Inserts

3xP3 promoter
tdTomato
Gypsy insulator
CTCF insulator
Y homology arm left
Y homology arm right

Use

Tags

Expression

Insect

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
AByF

Plasmid

#111084

Purpose

Integrates marker gene onto D. melanogaster Y chromosome

Depositor

Omar Akbari

Article

Buchman et al Insect Mol Biol. 2018 Aug 6. doi: 10.111

Inserts

Y homology arm left
gypsy insulator
3xP3 promoter
tdTomato
SV40 3'UTR
CTCF insulator
Y homology arm right

Use

Tags

Expression

Insect

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
UAS-fog

Plasmid

#41178

Depositor

Norbert Perrimon

Article

Hacker et al Genes Dev. 1998 Jan 15;12(2):274-84.

Insert

fog (fog Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Hsp70

Availability

Academic Institutions and Nonprofits only
795G

Plasmid

#205481

Purpose

Expresses dsRed and eGFP in both sexes.

Depositor

Omar Akbari

Article

Liu et al bioRxiv. 2023 Aug 14:2023.08.11.553026.

Insert

DsRed, eGFP

Use

Tags

Expression

Insect

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge