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Showing: 21 - 40 of 75 results

  6. GRM1-DuET

    Plasmid
    #213300
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    GRM1 (GRM1 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Availability
    Academic Institutions and Nonprofits only
    213300-plasmid-map-sequence-id-430906

    Enlarge

  7. GRM2-DuET

    Plasmid
    #213301
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    GRM2 (GRM2 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Availability
    Academic Institutions and Nonprofits only
    213301-plasmid-map-sequence-id-430907

    Enlarge

  8. GRM5-DuET

    Plasmid
    #213303
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    GRM5 (GRM5 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Availability
    Academic Institutions and Nonprofits only
    213303-plasmid-map-sequence-id-431813

    Enlarge

  9. GRM6-DuET

    Plasmid
    #213304
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    GRM6 (GRM6 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Availability
    Academic Institutions and Nonprofits only
    213304-plasmid-map-sequence-id-430908

    Enlarge

  10. GRM7-DuET

    Plasmid
    #213305
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    GRM7 (GRM7 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Availability
    Academic Institutions and Nonprofits only
    213305-plasmid-map-sequence-id-430909

    Enlarge

  11. pLenti6M CD38

    Plasmid
    #194125
    Purpose
    Expresses CD38 protein in mammalian cells
    Depositor
    Jun Liu
    Insert
    CD38 (CD38 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    194125-plasmid-map-sequence-id-380720

    Enlarge

  12. 5C5C

    Plasmid
    #69964
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    GRM7:DCC014-D04:C211307 (GRM7 Human)
    Use
    Baculovirus expression
    Tags
    His and TEV cleavage site
    Expression
    Mutation
    Promoter
    polyhedrin
    Availability
    Academic Institutions and Nonprofits only

  13. GRM7

    Plasmid
    #28110
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    GRM7:DCC014-D04:C211307 (GRM7 Human)
    Use
    Tags
    His tag with TEV site
    Expression
    Bacterial
    Mutation
    contains ligand binding domain, residues 37-513.
    Promoter
    Availability
    Industry, Academic Institutions, and Nonprofits

  14. GRM1

    Plasmid
    #25353
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    GRM1-C254S:L28-G518 (GRM1 Human)
    Use
    Tags
    His
    Expression
    Insect
    Mutation
    C254S. Contains amino acids L28-G518.
    Promoter
    Availability
    Industry, Academic Institutions, and Nonprofits

  15. GRM3 (3SM9)

    Plasmid
    #32870
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    GRM3 (GRM3 Human)
    Use
    Tags
    His tag with TEV cleavage and Honeybee melittin S…
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Industry, Academic Institutions, and Nonprofits

  16. pcDNA3_HLA-cmyc-GRM3 C127A

    Plasmid
    #140451
    Purpose
    Mammalian expression plasmid for c-myc-tagged mGluR3 C127A mutant
    Depositor
    Erik Procko
    Insert
    GRM3 (GRM3 Human)
    Use
    Tags
    Signal/leader sequence from HLA class I histocomp…
    Expression
    Mammalian
    Mutation
    Cysteine 127 to alanine
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    140451-plasmid-map-sequence-id-291874

    Enlarge

  17. pCEP4_HA-Halo-cmyc-GRM3 C127A

    Plasmid
    #140458
    Purpose
    Mammalian expression plasmid for N-terminal Halo- and c-myc-tagged mGluR3 C127A
    Depositor
    Erik Procko
    Insert
    GRM3 (GRM3 Human)
    Use
    Tags
    Halo, Signal/leader sequence from influenza hemag…
    Expression
    Mammalian
    Mutation
    Cysteine 127 to alanine, Threonine 174 to alanine
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    140458-plasmid-map-sequence-id-292298

    Enlarge

  18. pCEP4_HLA-flag-GRM3 C127A-VN

    Plasmid
    #140449
    Purpose
    Mammalian expression plasmid for FLAG-tagged mGluR3 fused to the N-terminal half of split fluorescent Venus (VN)
    Depositor
    Erik Procko
    Insert
    GRM3 (GRM3 Human)
    Use
    Tags
    FLAG tag, Signal/leader sequence from HLA class I…
    Expression
    Mammalian
    Mutation
    Cysteine 127 to alanine
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    140449-plasmid-map-sequence-id-292293

    Enlarge

  19. pCEP4_HLA-myc-GRM3 C127A-VC

    Plasmid
    #140450
    Purpose
    Mammalian expression plasmid for c-myc-tagged mGluR3 fused to the C-terminal half of split fluorescent Venus (VC)
    Depositor
    Erik Procko
    Insert
    GRM3 (GRM3 Human)
    Use
    Tags
    Signal/leader sequence from HLA class I histocomp…
    Expression
    Mammalian
    Mutation
    Cysteine 127 to alanine
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    140450-plasmid-map-sequence-id-292295

    Enlarge

  20. pcDNA3_HLA-cmyc-GRM3 C127A TMD I578A

    Plasmid
    #140452
    Purpose
    Mammalian expression plasmid for c-myc-tagged mGluR3 C127A and I578A mutant
    Depositor
    Erik Procko
    Insert
    GRM3 (GRM3 Human)
    Use
    Tags
    Signal/leader sequence from HLA class I histocomp…
    Expression
    Mammalian
    Mutation
    Cysteine 127 to alanine, isoluecine 578 to alanine
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    140452-plasmid-map-sequence-id-292301

    Enlarge

Showing: 21 - 40 of 75 results