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Addgene
Showing: 101 - 120 of 7103 results

  1. pcDNA3.3_SARS2_XBB.1.16

    Plasmid
    #201189
    Purpose
    expressing SARS-CoV-2 XBB.1.16 spike protein for pseudovirus production
    Depositor
    David Nemazee
    Insert
    Spike (XBB.1.16) (S SARS-CoV-2)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    E180V, T(K)478R on the basis of XBB.1.5
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    201189-plasmid-map-sequence-id-393640

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  3. pcDNA3.3_SARS2_BA.2.75.2

    Plasmid
    #194498
    Purpose
    expressing SARS-CoV-2 BA2.75.2 spike protein for pseudovirus production
    Depositor
    David Nemazee
    Insert
    Spike (BA.2.75.2) (S SARS-CoV-2)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    R346T, F486S, D1199N and c-terminal 18aa del on t…
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    194498-plasmid-map-sequence-id-381635

    Enlarge

  5. pcDNA3.3_SARS2_XBB.1.5

    Plasmid
    #196585
    Purpose
    expressing SARS-CoV-2 XBB.1.5 spike protein for pseudovirus production
    Depositor
    David Nemazee
    Insert
    Spike (XBB.1.5)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    G252V, F486P on the basis of XBB
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    196585-plasmid-map-sequence-id-386779

    Enlarge

  6. pcDNA3.3_SARS2_BA.2.86

    Plasmid
    #208441
    Purpose
    expressing SARS-CoV-2 BA.2.86 spike protein for pseudovirus production
    Depositor
    David Nemazee
    Insert
    Spike (BA.2.86) (S SARS-CoV-2)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    see GISAID sequence
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    208441-plasmid-map-sequence-id-410934

    Enlarge

  10. NSarQ27G-VMAn-FKBP

    Plasmid
    #70230
    Purpose
    Expresses N-terminal portion of alpha sarcin with Q27G mutation along with VMA intein for protein splicing
    Depositor
    Perry Howard
    Article
    Alford et al Mol Biosyst. 2014 Apr;10(4):831-7. doi:
    Insert
    alpha Sarcin (N-terminal portion)
    Use
    Tags
    FK506- and rapamycin-binding protein (FKBP), Link…
    Expression
    Mammalian
    Mutation
    Changed Gln 27 to Gly
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only

  11. pDONR221-SAR1-no stop

    Plasmid
    #67345
    Purpose
    hSar1 without stop codon in Gateway Entry vector
    Depositor
    Richard Kahn
    Insert
    SAR1 (SAR1A Human)
    Use
    Gateway entry vector
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    None
    Availability
    Academic Institutions and Nonprofits only

  12. pDONR221-SARA-no stop

    Plasmid
    #67347
    Purpose
    hSara without stop codon in Gateway Entry vector
    Depositor
    Richard Kahn
    Insert
    SARA (SAR1B Human)
    Use
    Gateway entry vector
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    None
    Availability
    Academic Institutions and Nonprofits only

  13. pDSHA-SAR1-Myc

    Plasmid
    #67367
    Purpose
    Mammalian expression of hSar1 with C-term Myc tag
    Depositor
    Richard Kahn
    Insert
    SAR1 (SAR1A Human)
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only

  14. pDSHA-SARA-Myc

    Plasmid
    #67368
    Purpose
    Mammalian expression of hSara with C-term Myc tag
    Depositor
    Richard Kahn
    Insert
    SARA (SAR1B Human)
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only

  15. pDEST47-SAR1-GFP

    Plasmid
    #67409
    Purpose
    Mammalian expression of hSar1 with C-term GFP tag
    Depositor
    Richard Kahn
    Insert
    SAR1 (SAR1A Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only

  16. pDEST47-SARA-GFP

    Plasmid
    #67410
    Purpose
    Mammalian expression of hSara with C-term GFP tag
    Depositor
    Richard Kahn
    Insert
    SARA (SAR1B Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only

  17. pDEST40-SAR1-V5-His6

    Plasmid
    #67451
    Purpose
    Mammalian expression of hSar1 with C-term V5 and His6 tag
    Depositor
    Richard Kahn
    Insert
    SAR1 (SAR1A Human)
    Use
    Tags
    His and V5
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only

  18. pDEST40-SARA-V5-His6

    Plasmid
    #67452
    Purpose
    Mammalian expression of hSara with C-term V5 and His6 tag
    Depositor
    Richard Kahn
    Insert
    SARA (SAR1B Human)
    Use
    Tags
    His and V5
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
Showing: 101 - 120 of 7103 results