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pcDNA3 Flag beta-2-adrenergic-receptor

Plasmid

#14697

Depositor

Robert Lefkowitz

Article

Tang et al Proc Natl Acad Sci U S A. 1999 Oct 26. 9

Insert

B2AR (ADRB2 Human)

Tags

Flag

Expression

Mammalian

Available Since

June 19, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 MCS-BirA(R118G)-HA

Plasmid

#36047

Purpose

To fuse your protein of interest to the N-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID); HA tag

Depositor

Kyle Roux

Article

Roux et al J Cell Biol. 2012 Mar 12.

Has Service

Cloning Grade DNA

Insert

BirA(R118G)-HA

Tags

HA

Expression

Mammalian

Mutation

R118G (highly promiscuous form)

Available Since

April 13, 2012

Availability

Academic Institutions and Nonprofits only

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TFORF2249

Plasmid

#143184

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

TCF12 (TCF12 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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pSBbi-NLSmCherryNLS Clover-HDHB

Plasmid

#190223

Purpose

Fluorescent cell cycle reporter

Depositor

Laura Heiser

Article

Gross et al Nat Commun. 2023 Jun 10;14(1):3450. doi:

Inserts

Human DNA Helicase B (amino acids 994-1087) (HELB Human)
NLS-mCherry-NLS-P2A-Puromycin

Use

Transposon

Tags

Clover fluorescent protein

Expression

Mammalian

Mutation

Amino Acids 994-1087

Promoter

EF1a and RPBSA

Available Since

Sept. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pET28-hXRCC1

Plasmid

#70759

Purpose

bacterial expression of human XRCC1

Depositor

Primo Schaer

Article

Schuermann et al Nucleic Acids Res. 2016 Jan 4. pii: gkv1

Insert

XRCC1 (XRCC1 Human)

Tags

6-HIS

Expression

Bacterial

Promoter

T7/lac operon

Available Since

Jan. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv2_mSTING_gRNA_2

Plasmid

#196626

Purpose

Knock-out STING in murine cells, gRNA 2.

Depositor

Nicolas Manel

Article

Jneid et al Sci Immunol. 2023 Jan 13;8(79):eabn6612.

Insert

mSTING gRNA 2 (Sting1 Mouse)

Use

CRISPR and Lentiviral

Mutation

WT

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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MSCV-AML1/ETO-IRES-GFP

Plasmid

#60832

Purpose

AML1/ETO9a cDNA was generated by PCR from full-length human AML1/ETO-IRES-GFP and subcloned into MSCV-IRES-GFP

Depositor

Scott Lowe

Article

Zuber et al Genes Dev. 2009 Apr 1;23(7):877-89. doi:

Insert

AML1/ETO (RUNX1, RUNX1T1 Human)

Use

Retroviral

Available Since

April 19, 2016

Availability

Academic Institutions and Nonprofits only

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Cbh_v5 AAV-CBE C-terminal

Plasmid

#137176

Purpose

AAV genome: expresses the C-terminal of v5 AAV-CBE from the Cbh promoter, and U6-sgRNA

Depositor

David Liu

Article

Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

Insert

v5 AAV-CBE C-terminal; U6-protospacer

Use

AAV

Promoter

Cbh

Available Since

Jan. 28, 2020

Availability

Academic Institutions and Nonprofits only

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YPet-N1

Plasmid

#54637

Purpose

Localization: N1 Cloning Vector, Excitation: 513, Emission: 527

Depositor

Patrick Daugherty, Michael Davidson

Article

Nguyen et al Nat Biotechnol. 2005 Mar;23(3):355-60. d

Type

Empty backbone

Tags

YPet

Expression

Mammalian

Available Since

June 20, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pZac2.1-gfaABC1D-PinkyCaMP

Plasmid

#232859

Purpose

An AAV vector expressing the mScarlet based calcium indicator PinkyCaMP under gfaABC1D promoter

Depositor

Olivia Masseck

Article

Fink et al bioRxiv [Preprint]. 2025 Jan 11:2024.12.

Insert

PinkyCaMP

Use

AAV

Expression

Mammalian

Promoter

gfaABC1D

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-CAG-GFP

Plasmid

#28014

Depositor

Karel Svoboda

Article

Mao et al Neuron. 2011 Oct 6;72(1):111-23.

Insert

eGFP

Use

AAV; Adeno associate viral

Available Since

Nov. 30, 2011

Availability

Academic Institutions and Nonprofits only

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mEmerald-Coronin1B-N-10

Plasmid

#54050

Purpose

Localization: Cytoskeleton, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Insert

Coronin1B (CORO1B Human)

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

July 2, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pFA6a-link-ymScarletI3-URA3

Plasmid

#224075

Purpose

Yeast tagging vector to create a gene fusion with yeast optimized mScarletI3 with URA3 selection

Depositor

Bas Teusink

Insert

ymScarletI3

Tags

ymScarletI3

Expression

Yeast

Available Since

Feb. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pTriEx-mCherry-LOV2

Plasmid

#81041

Purpose

Mammalian or insect expression for wt LOV2

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Insert

LOV2

Tags

mCherry

Expression

Bacterial, Insect, and Mamm…

Promoter

CMV, p10

Available Since

Aug. 17, 2016

Availability

Academic Institutions and Nonprofits only

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SspBmicro-mCh-p60

Plasmid

#190168

Purpose

Expresses SspBmicro-mCh-p60, a recruitable katanin for microtubule severing, in mammalian cells.

Depositor

Anna Akhmanova

Article

Meiring et al Curr Biol. 2022 Nov 7;32(21):4660-4674.e

Insert

SspBmicro-mCherry-p60

Tags

SspBmicro-mCherry

Expression

Mammalian

Available Since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pmCherry-ATG5

Plasmid

#13095

Depositor

Roberta Gottlieb

Article

Hamacher-Brady et al Cell Death Differ. 2007 Jan;14(1):146-57

Insert

ATG5 (Atg5 Mouse)

Tags

mCherry

Expression

Mammalian

Available Since

Nov. 15, 2006

Availability

Academic Institutions and Nonprofits only

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pEFIRES-P-EGFP-ADRP

Plasmid

#87157

Purpose

Fluorescent protein localized to lipid droplets (LDs)

Depositor

Elina Ikonen

Article

Salo et al EMBO J. 2016 Dec 15;35(24):2699-2716. Ep

Insert

ADRP (PLIN2 Human)

Tags

EGFP

Expression

Mammalian

Promoter

mCherry-R TTGGTCACCTTCAGCTTGG

Available Since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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lucMAPT-30D

Plasmid

#214672

Purpose

MAPT Exon 10 reporter containing the MS2 hairpin 30 base pairs downstream of the 5′ splice site

Depositor

Eugene Yeo

Article

Schmok et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

MAPT (MAPT Human)

Tags

Firefly Luciferase, Renilla Luciferase, and V5

Expression

Mammalian

Mutation

Contains Exon 9, Exon 10 with a mutation to intro…

Promoter

CMV

Available Since

March 15, 2024

Availability

Academic Institutions and Nonprofits only

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lucMAPT-30U

Plasmid

#214673

Purpose

MAPT Exon 10 reporter containing the MS2 hairpin 30 base pairs upstream of the 3′ splice site

Depositor

Eugene Yeo

Article

Schmok et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

MAPT (MAPT Human)

Tags

Firefly Luciferase, Renilla Luciferase, and V5

Expression

Mammalian

Mutation

Contains Exon 9, Exon 10 with a mutation to intro…

Promoter

CMV

Available Since

March 15, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV.GFAP.Cre.WPRE.hGH

Plasmid

#105550

Purpose

AAV expression of Cre from GFAP promoter

Depositor

James M. Wilson

Has Service

AAV PHP.eB and AAV5

Insert

Cre

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

GFAP

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-6Xhis-human SUMO 3

Plasmid

#133772

Purpose

express 6Xhis- SUMO 3 protein

Depositor

Martine Roussel

Article

Tago et al Proc Natl Acad Sci U S A. 2005 May 24. 1

Insert

SUMO 3 (SUMO3 Human)

Tags

6XHis

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2019

Availability

Academic Institutions and Nonprofits only

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TFORF0459

Plasmid

#142266

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

CREB5 (CREB5 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

June 8, 2023

Availability

Academic Institutions and Nonprofits only

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pBMN(CMV-copGFP-Luc2-Puro)

Plasmid

#80389

Purpose

To make lentiviral vector expressing copGFP, firefly Luciferase and puromycin selection marker

Depositor

Magnus Essand

Article

Jin et al EMBO Mol Med. 2016 May 6. pii: e20150586

Insert

copGFP-T2A-Luc2-F2A-Puromycin

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 6, 2016

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-EGFP

Plasmid

#114213

Purpose

AAV vector (control) that targets expression of EGFP to neurons

Depositor

Xue Han

Article

Keaveney et al Cell Rep. 2018 Jul 10;24(2):294-303. doi

Insert

Enhanced Green Fluorescent Protein

Use

AAV and Mouse Targeting

Tags

None

Expression

Mammalian

Mutation

None

Promoter

human synapsin (hSyn)

Available Since

Jan. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-hSFTPCpromoter(2kb)-EGFP-EF1a-TagRFP

Plasmid

#201681

Purpose

Lentiviral vector for human SFTPC promoter (2kb)-driven expression of EGFP and EF1a-driven TagRFP expression

Depositor

Emma Rawlins

Article

Lim et al bioRxiv [Preprint]. 2023 Sep 4:2023.08.3

Inserts

SFTPC promoter region (2kb upstream)
TagRFP

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HNF1B-WT-Flag-UTR

Plasmid

#183239

Purpose

Expression of FLAG tagged HNF1B

Depositor

Jorge Ferrer

Article

Beucher et al Nat Cell Biol. 2022 Oct 6. pii: 10.1038/

Insert

HNF1 homeobox B (HNF1B Human)

Tags

FLAG tag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag MKK3b(Glu)

Plasmid

#50449

Purpose

activates p38 in the signaling pathway

Depositor

Roger Davis

Article

Enslen et al EMBO J. 2000 Mar 15. 19(6):1301-11.

Insert

MKK3b (MAP2K3 Human)

Tags

Flag

Expression

Mammalian

Mutation

changed Serine 218 to Glutamic Acid, changed Thre…

Promoter

CMV

Available Since

Jan. 22, 2014

Availability

Academic Institutions and Nonprofits only
fabp10a:lox2272-loxp-nls-mTagBFP2-stop-lox2272-H2B-mGL-stop-loxp-mCherry-NTR; cryaa:mCherry

Plasmid

#230043

Purpose

Partial ablation and subsequent lineage tracing of regenerating hepatocytes in zebrafish.

Depositor

Sumeet Pal Singh

Article

Eski et al Nat Commun. 2025 Jun 6;16(1):5260. doi:

Insert

lox2272-loxp-nls-mTagBFP2-stop-lox2272-H2B-mGL-stop-loxp-mCherry-NTR

Use

Cre/Lox; Zebrafish expression

Promoter

fabp10a

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pET-SUMO-hGSDMD

Plasmid

#111559

Purpose

Express human GSDMD in E.coli

Depositor

Hongbo Luo

Article

Kambara et al Cell Rep. 2018 Mar 13;22(11):2924-2936.

Insert

Gasdermin D (GSDMD Human)

Tags

His, SUMO

Expression

Bacterial

Promoter

T7

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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TFORF2476

Plasmid

#144030

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

ZFHX2 (ZFHX2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

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