159,258 results

1. 478 p5Efli1ep

   Plasmid

   #31160

   Depositor

   Nathan Lawson

   Article

   Villefranc et al Dev Dyn. 2007 Nov . 236(11):3077-87.

   Insert

   chimeric fli1ep enhancer/promoter fragment
   

   Use

   Entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 16, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pU6_HEK3+1T>A_(PP7-C4-Q1)

   Plasmid

   #232437

   Purpose

   pegRNA with optimized 3' modifications to facilitate a +1 T to A prime edit in the HEK3 locus

   Depositor

   Gil Westmeyer

   Article

   Geilenkeuser et al Cell, April 2025. doi: 10.1016/j.cell.20

   Insert

   3'-PP7-tagged pegRNA for rd12 correction driven by human U6 promoter
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-flag-YTHDF2

   Plasmid

   #52300

   Purpose

   full length YTHDF2 with N-terminal flag tag for mammalian cell expression

   Depositor

   Chuan He

   Article

   Wang et al Nature. 2014 Jan 2;505(7481):117-20. doi

   Insert

   YTHDF2 (YTHDF2 Human)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Promoter

   T7

   Available since

   April 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLENTI_TetOn_Hu_BIRC5(WT)

   Plasmid

   #136348

   Purpose

   Lentiviral inducible expression (TetOn) of human BIRC5

   Depositor

   Guillermo de Cárcer

   Insert

   BIRC5 (BIRC5 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. T4 td upstream intron-PABP

   Plasmid

   #189972

   Purpose

   Part 1 plasmid to introduce the T4 td upstream intron and PABP spacer into circRNAs

   Depositor

   Howard Chang

   Article

   Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

   Insert

   T4 td upstream intron-PABP
   

   Use

   Cloning

   Tags

   Expression

   Mutation

   Promoter

   N/A

   Available since

   Sept. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. HBA1 full 3'UTR-T4 td downstream intron

   Plasmid

   #189974

   Purpose

   Part 6 plasmid to introduce the HBA1 full 3'UTR and T4 td downstream intron into circRNAs

   Depositor

   Howard Chang

   Article

   Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

   Insert

   HBA1 full 3'UTR-T4 td downstream intron
   

   Use

   Cloning

   Tags

   Expression

   Mutation

   Promoter

   N/A

   Available since

   Sept. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV-mOXT-hM3Dq-mCherry-WPRE

   Plasmid

   #70717

   Purpose

   Expresses DREADDs Gq receptor under oxytocin promoter control

   Depositor

   Daniel Geschwind

   Article

   Penagarikano et al Sci Transl Med. 2015 Jan 21;7(271):271ra

   Insert

   hM3Dq-mCherry
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   mouse oxytocin

   Available since

   Jan. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLVX-Puro-TDP-43-Q331K

   Plasmid

   #133761

   Purpose

   Lentiviral expression of human TDP-43 Q331K disease mutant in mammalian cells.

   Depositor

   Shawn Ferguson

   Article

   Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

   Insert

   TDP-43 (TARDBP Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Changed glutamine 331 to lysine.

   Promoter

   CMV

   Available since

   Dec. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGFP-C1 Lifeact-EGFP

   Plasmid

   #58470

   Purpose

   Expresses a cytoplasmic actin filament reporter, the peptide Lifeact, on a CMV promoter

   Depositor

   Dyche Mullins

   Article

   Belin et al Bioarchitecture. 2015 Aug 28:1-14.

   Insert

   Lifeact
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti-EF1a-Ngn3ER-PuroR-mCherry

    Plasmid

    #197385

    Purpose

    To establish Ngn3ER cell line for endocrine differentiation (4-hydroxytamoxifen activation, Puromycin resistant)

    Depositor

    Qiao Zhou

    Article

    Huang et al Nat Cell Biol. 2023 Apr 27. doi: 10.1038

    Insert

    Ngn3ER-2A-PuroR-2A-mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. Lenti-CMV-PRB

    Plasmid

    #206165

    Purpose

    pMVP Gateway destination vector (3rd generation lentiviral vector) containing human full length PRB gene

    Depositor

    Renee van Amerongen

    Article

    Aarts et al J Mammary Gland Biol Neoplasia. 2023 Nov

    Insert

    Progesteron receptor B (PGR Human)
    

    Use

    Lentiviral; Pmvp gateway destination vector

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. M3-PAmcherry1

    Plasmid

    #114189

    Purpose

    studying clustering of M3 receptors by superresolution microscopy (PALM)

    Depositor

    Aleksandra Radenovic

    Insert

    Muscarin acetylcholine receptor 3 (CHRM3 Human)
    

    Use

    Tags

    3xHA and PAmCherry

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSBbi-GB

    Plasmid

    #60520

    Purpose

    SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: GFP and blasticidin resistance gene

    Depositor

    Eric Kowarz

    Article

    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

    Type

    Empty backbone

    Use

    Transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a/RPBSA

    Available since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pIS2

    Plasmid

    #12177

    Depositor

    David Bartel

    Article

    Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

    Type

    Empty backbone

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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15. pT3-EF1aH Yap S127A

    Plasmid

    #86497

    Purpose

    Mammalian expression of FLAG-tagged Yap containing S127A mutation

    Depositor

    Xin Chen

    Article

    Tschaharganeh et al Gastroenterology. 2013 Jun;144(7):1530-1

    Insert

    Yes associated protein 1 (YAP1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S127A

    Promoter

    EF1a

    Available since

    Feb. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFB2

    Plasmid

    #195282

    Purpose

    Bicistronic DTR and Puromycin resistance for positive/negative selection

    Depositor

    Scott Lowe

    Article

    Barriga et al Nat Cancer. 2022 Nov;3(11):1367-1385. do

    Insert

    DTR 2A Puro (HBEGF Human, Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Jan. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Ac5-STABLE2-neo

    Plasmid

    #32426

    Depositor

    Rosa Barrio, James Sutherland

    Article

    Gonzalez et al Sci Rep. 2011;1:75. doi: 10.1038/srep000

    Insert

    Flag-mCherry-T2A-GFP-T2A-neo
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Actin5C

    Available since

    Oct. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPB-cT3G-cERP2-HMGB2

    Plasmid

    #222532

    Purpose

    PiggyBac transposon plasmid for doxycycline inducible expression of HMGB2

    Depositor

    George Church

    Article

    Pierson Smela et al bioRxiv 2024.05.31.596483

    Insert

    HMGB2 (HMGB2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. ptdTomato-SMCR8

    Plasmid

    #83438

    Purpose

    Expresses SMCR8-tdTomato in mammalian cells

    Depositor

    Shawn Ferguson

    Article

    Amick et al Mol Biol Cell. 2016 Aug 24. pii: mbc.E16

    Insert

    Human SMCR8 (SMCR8 Human)
    

    Use

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTW1169

    Plasmid

    #236583

    Purpose

    TRV1

    Depositor

    Steven Jacobsen

    Article

    Weiss et al Nat Plants. 2025 Apr 22. doi: 10.1038/s4

    Insert

    TRV1
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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21. pGGAselect

    Plasmid

    #195714

    Purpose

    pGGAselect is a cloning vector for Golden Gate Assembly using BsaI, BsmBI, and BbsI Type IIS restriction enzymes.

    Depositor

    Eric Cantor

    Article

    Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 24, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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22. Gq-CASE

    Plasmid

    #168125

    Purpose

    Encodes a BRET-based activity sensor for the heterotrimeric G protein Gq. Composed of the subunits G alpha q (GNAQ) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).

    Depositor

    Gunnar Schulte

    Article

    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.

    Insert

    GNAQ, GNB3, GNG9 (GNAQ, GNB3, GNGT2 Human)
    

    Use

    Luciferase

    Tags

    cpVenus on GNG9

    Expression

    Mammalian

    Mutation

    NLuc is inserted at F124/E125 within GNAQ

    Promoter

    Available since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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23. EK0479 CMV-TO-VEGF-A(165) (FLP-IN)

    Plasmid

    #233384

    Purpose

    Secreted human VEGF-A

    Depositor

    Xiaojing Gao

    Article

    Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

    Insert

    VEGF-A (VEGFA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. PB53x EF1-Dendra2-RPB1Amr

    Plasmid

    #81228

    Purpose

    expresses Dendra2-Rpb1 fusion in mammalian cells. Possible to insert into the genome using the Piggibac system

    Depositor

    Timothée Lionnet

    Article

    Cho et al Elife. 2016 May 3;5. pii: e13617. doi: 1

    Insert

    Dendra2 - Rpb1 (alpha amanitin resistant) (POLR2A Human)
    

    Use

    Tags

    Dendra2

    Expression

    Mammalian

    Mutation

    N792D alpha amanitin resistance mutation (Bartolo…

    Promoter

    EF1a

    Available since

    Nov. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLKO-p53-shRNA-427

    Plasmid

    #25636

    Depositor

    Todd Waldman

    Article

    Kim et al Mol Cell Biol. 2007 Jan . 27(2):662-77.

    Insert

    p53-shRNA-427 (TP53 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 21, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pT3-EF1aH NICD1

    Plasmid

    #86500

    Purpose

    Mammalian expression of Myc tagged NICD1

    Depositor

    Xin Chen

    Article

    Fan et al J Clin Invest. 2012 Aug 1;122(8):2911-5.

    Insert

    Notch1 (C-term) (Notch1 Mouse)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Cytoplasmic domain only (AA 1753-2531)

    Promoter

    EF1a

    Available since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA3-OVA

    Plasmid

    #64599

    Purpose

    Expression of chicken ovalbumin as model antigen for study of MHC class I and class II presentation

    Depositor

    Martin Zenke, Sandra Diebold

    Article

    Diebold et al Gene Ther. 2001 Mar;8(6):487-93.

    Insert

    Ovalbumin
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. lentiCRISPRv2FE-ABE8e-SpRY

    Plasmid

    #213008

    Purpose

    A lentiviral vector expressing the ABE8e-SpRY base editor and an sgRNA cloning site

    Depositor

    Richard Sherwood

    Article

    Ryu et al Nat Genet. 2024 Apr 24. doi: 10.1038/s41

    Insert

    ABE8e-SpRY-D10A
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A nickase variant of SpRY

    Promoter

    Ef-1a

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pBSTNAV

    Plasmid

    #45801

    Depositor

    Luc Ponchon

    Article

    Ponchon et al Nucleic Acids Res. 2013 Aug;41(15):e150.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. evoBxb1

    Plasmid

    #222338

    Purpose

    Plasmid for expressing evolved Bxb1 in the mammalian cells

    Depositor

    David Liu

    Article

    Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

    Insert

    Codon-optimized evolved Bxb1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge