27,466 results
-
-
pcDNA3-GFP-MT2A
Plasmid#11613DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmetallothionein 2A (MT2A Human)
TagsGFPExpressionMammalianAvailable sinceJuly 18, 2007AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGAL4-CREB S133A
Plasmid#15222DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCREB S133A (Creb1 Rat)
TagsGal4ExpressionMammalianMutationSerine 133 mutated to AlanineAvailable sinceJune 29, 2007AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PECAM-eGFP
Plasmid#14688DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPECAM promoter/enhancer (Pecam1 Mouse)
TagsEGFPExpressionMammalianAvailable sinceMay 15, 2007AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMT2 GATA1
Plasmid#13626DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGATA1 (Gata1 Mouse)
ExpressionMammalianAvailable sinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDsRed1-N1 RanGAP
Plasmid#13386DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRanGAP (RANGAP1 Human)
TagsDsRed1ExpressionMammalianAvailable sinceNov. 16, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
1306 pSG5L HA FKHR wt
Plasmid#10693DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertFKHR (FOXO1 Human)
TagsHAExpressionMammalianAvailable sinceDec. 7, 2005AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pJRH-1345 U6-B2M sgRNA Gag-Cas9 v2
Plasmid#201916PurposeCas9-EDV production plasmid. Expresses the v2 Gag-Cas9 polypeptide (CAG promoter) and B2M sgRNA (human U6 promoter, 5’-GAGTAGCGCGAGCACAGCTA)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsGag-Cas9 v2
B2M sgRNA (Target-specific sequence: 5'- GAGTAGCGCGAGCACAGCTA)
ExpressionMammalianPromoterCAG and Human U6Available sinceAug. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
TRUPATH Triple (bidirectional) GasS
Plasmid#196055PurposeEncodes a G alpha subunit (GNAS2) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteinsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsUseLuciferaseTagsGFP2 and GSAG linkerExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pB80-hKif5b(1-560)rigor-2xmNeongreen
Plasmid#174649PurposeLive-cell marker for stable microtubulesDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthKif5B(1-560)rigor-2xmNeongreen (KIF5B Human)
TagsmNeongreen-mNeongreenExpressionMammalianMutationrigor mutation G234APromoterChicken beta-actinAvailable sinceMarch 7, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDAC446
Plasmid#195239PurposeMammalian expression of Csm complex from separate promoters; GFP backbone (Used for RNA knockdown)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertFLAG-NLS-Csm1; FLAG-NLS-Csm2; FLAG-NLS-Csm3; FLAG-NLS-Csm4; FLAG-NLS-Csm5; FLAG-NLS-Cas6; crRNA; GFP
ExpressionMammalianAvailable sinceJan. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
mito-meGFP
Plasmid#172481PurposemeGFP targeted to the mitochondrial matrix with a human COX8 presequenceDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMito (COX8A Human)
TagsmeGFPExpressionMammalianPromoterCMVAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
G13-CASE
Plasmid#168127PurposeEncodes a BRET-based activity sensor for the heterotrimeric G protein G13. Composed of the subunits G alpha 13 (GNA13) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAUseLuciferaseTagscpVenus on GNG9ExpressionMammalianMutationNLuc is inserted at F125/D126 within GNA13Available sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-CreEGFP
Plasmid#165140PurposeExpression of Cre recombinase fused with EGFP in C-terminalDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCre recombinase
TagsEGFPExpressionMammalianPromoterCMVAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Gαi*-BERKY3
Plasmid#158198PurposeBRET biosensor for detection of Gαi-GTP in mammalian expression vector pcDNA3.1(+)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLyn11-Nluc-ER/K linker-3xYFP-KB1753-myc
TagsmycExpressionMammalianPromoterCMVAvailable sinceSept. 17, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
ABE8.8-m
Plasmid#136294Purposeexpresses ABE8.8-m in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertABE8.8-m
TagsC-terminal BPNLSExpressionMammalianMutationD10A in S. pyogenes Cas9, TadA mutations describe…Available sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CMV-OMMjRCaMP-P2A-mito4xGCaMP6f
Plasmid#127872PurposeDual expression vector for mitochondrial matrix and outer membrane Ca2+ sensorsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertOuter membrane localized jRCaMP1b - P2A - mito4xGCaMP6f
ExpressionMammalianPromoterCMVAvailable sinceNov. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR_P2R-P3_R2-Turbo-mVenus-STOP-L3
Plasmid#127354Purposegateway entry vector for making C-terminal TurboID-mVenus fusion with nuclear and non-nuclear proteinsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTurboID (BirA mutant)
UseGateway CloningTagsGS linker, V5, and mVenusMutationQ65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…Promoterno promoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSH-EFIRES-P-Seipin-miniIAA7-3XFlag
Plasmid#129722PurposeAAVS1 safe harbor targeting vector expressing miniIAA7-3XFlag tagged SeipinDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSeipin (BSCL2 Human)
UseCRISPR and TALEN; Human safe harbor locus (a avs1…TagsminiIAA7-3XFlagExpressionMammalianMutationsilent mutationsPromoterEF1aAvailable sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pH2B-miRFP670nano
Plasmid#127438PurposeHistone 2B labeling with near-infrared fluorescent protein miRFP670nanoDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertH2B-miRFP670nano (H2BC11 Synthetic, Human)
TagsmiRFP670nanoExpressionMammalianPromoterCMVAvailable sinceJuly 17, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by
