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27,466 results

Showing: 4821 - 4840 of 27466 results

  2. pcDNA3-ExRai-AMPKAR(T/A)

    Plasmid
    #192447
    Purpose
    Non-phosphorylatable negative-control mutant of ExRai-AMPKAR.
    Depositor
    Jin Zhang
    Publication
    Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ExRai-AMPKAR(T/A)
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag
    Expression
    Mammalian
    Mutation
    Thr 6 in AMPK substrate mutated to Ala.
    Promoter
    CMV
    Available since
    Dec. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    192447-plasmid-map-sequence-id-379141

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  3. pCBh-NLS_dCas13d_HA_NLS-pA-U6-DR-BpiI-BpiI-pSV40-EGFP-pA-pSV40-mCherry-pA

    Plasmid
    #191797
    Purpose
    vector for encoding a human codon-optimized dead RfxCas13d (dCas13d) driven by CBh promoter, guide RNAs compatible with RfxCas13d driven by hU6, EGFP driven by SV40 promoter, and mCherry driven by SV40 promoter
    Depositor
    Huawei Tong
    Publication
    Tong et al Nat Biotechnol. 2022 Aug 11. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    humanized dCas13d
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    R239A, H244A, R858A, H863A
    Promoter
    CBh, SV40, hU6
    Available since
    Oct. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    191797-plasmid-map-sequence-id-375579

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  5. pDAS12489_PEAR-GFP_2in1_2.0

    Plasmid
    #177186
    Purpose
    PEAR-GFP3 plasmid with a pegRNA that targets its own plasmid
    Depositor
    Ervin Welker
    Publication
    Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    EGFP split with an intron between amino acids 95-96
    pegRNA targeting its own plasmid
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    disrupted 5' splice site
    Promoter
    CMV and U6
    Available since
    Feb. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    177186-plasmid-map-sequence-id-348478

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  6. pcDNA3.1_crGE

    Plasmid
    #172933
    Purpose
    Crowding FRET sensor with mCerulean3 and mCitrine for mammalian cell expression. Linker comprises 2 helices and 3 GSG domains.
    Depositor
    Arnold Boersma
    Publication
    Boersma et al Nat Methods. 2015 Mar;12(3):227-9, 1 p f
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CrowdingSensorGE
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    172933-plasmid-map-sequence-id-339481

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  7. pSEP-LAMP1-RFP

    Plasmid
    #167970
    Purpose
    Expresses the chimeric construct SEP-LAMP1-RFP where superecliptic pHluorin (SEP) is fused to the luminal domain of LAMP1-RFP
    Depositor
    Juan Bonifacino
    Publication
    Farias et al Proc Natl Acad Sci U S A. 2017 Apr 4;114
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    LAMP1 (Lamp1 Rat)
    Tags
    SEP and mRFP
    Expression
    Mammalian
    Mutation
    D50E
    Promoter
    CMV
    Available since
    April 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    167970-plasmid-map-sequence-id-331200

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  9. Tubulin-mGreenLantern

    Plasmid
    #164460
    Purpose
    Localization of mGreenLantern fluorescent protein to tubulin
    Depositor
    Gregory Petsko
    Publication
    Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mGreenLantern
    Tags
    Tubulin
    Expression
    Mammalian
    Mutation
    Clover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…
    Promoter
    CMV
    Available since
    Feb. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    164460-plasmid-map-sequence-id-322770

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  14. pcDNA3.1.Gαs-LgB113

    Plasmid
    #134362
    Purpose
    Nanoluc complementation assay. Expression of Gαs protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 113 and 114 of Gαs. Addition of the HA epitope at N terminus of Gαs.
    Depositor
    Julien Hanson
    Publication
    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Gαs-LgB113 (GNAS Human)
    Tags
    HA
    Expression
    Bacterial and Mammalian
    Promoter
    T7
    Available since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    134362-plasmid-map-sequence-id-306402

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  16. pCAG-mEGFP-CaMKIIa

    Plasmid
    #127389
    Purpose
    Fluorescent reporter for Ca2+/calmodulin-dependent protein kinase II alpha
    Depositor
    Ryohei Yasuda
    Publication
    Chang et al Nat Commun. 2019 Jun 25;10(1):2784. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    calcium/calmodulin-dependent protein kinase II alpha (Camk2a Rat)
    Tags
    mEGFP
    Expression
    Mammalian
    Promoter
    pCAG
    Available since
    Nov. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    127389-plasmid-map-sequence-id-250426

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  17. mCh-KIF5B*-strep

    Plasmid
    #120164
    Purpose
    Expresses a chimera of mCherry (fluorescent tag), a KIF5B-based anterograde motor and streptavidin
    Depositor
    Juan Bonifacino
    Publication
    Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    kinesin family member 5B (Kif5b Mouse)
    Tags
    Streptavidin and mCherry
    Expression
    Mammalian
    Mutation
    Truncated KIF5B: 1-555 aa
    Promoter
    CMV
    Available since
    July 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    120164-plasmid-map-sequence-id-247489

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Showing: 4821 - 4840 of 27466 results