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27,467 results

Showing: 9201 - 9220 of 27467 results

  1. pCAG-CBE4max-xCas9(3.7)-P2A-EGFP (RTW4637)

    Plasmid
    #140000
    Purpose
    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with xCas9(3.7)(D10A/A262T/R324L/S409I/E480K/E543D/M694I/E1219V) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized CBE4max xCas9(3.7) with P2A-EGFP
    Use
    CRISPR
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9=D10A; xCas9(3.7)=A262T/R324L/S409I/E480K/…
    Promoter
    CAG
    Available since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    140000-plasmid-map-sequence-id-275479

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  2. PL-5LTR-GW-A

    Plasmid
    #138480
    Purpose
    For expression of ribozyme-flanked sgRNA after the Gateway (GW) LR cloning.
    Depositor
    Akitsu Hotta
    Publication
    Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Gateway attR1/R2 cassette with chloramphenicol resistance and ccdB genes
    Expression
    Mammalian
    Available since
    March 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    138480-plasmid-map-sequence-id-275574

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  4. SpCas9-HF1-plus

    Plasmid
    #126768
    Purpose
    Expression plasmid for human codon-opt. increased fidelity SpCas9-HF1-plus (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as SpCas9-HF1
    Depositor
    Ervin Welker
    Publication
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9-HF1-plus
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A; Q695A; Q926A; amino acids 1005-1013 replac…
    Promoter
    CBh
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126768-plasmid-map-sequence-id-410966

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  6. pXGFPC-5 Pxyl-dcas9 (Sth3)

    Plasmid
    #133317
    Purpose
    expresses dcas9 (Streptococcus thermophilus #3); repressed with 0.2% glucose, induced with 0.3% xylose; for homologous recombination at the xyl locus in Caulobacter crescentus; tetracycline resistance
    Depositor
    Michael Laub
    Publication
    Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dcas9 (Streptococcus thermophilus #3)
    Use
    Unspecified
    Promoter
    xylose
    Available since
    Jan. 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    133317-plasmid-map-sequence-id-266835

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  8. pTol2-CreLite

    Plasmid
    #131783
    Purpose
    Tol2 destination vector carrying Ubi:CreLite with mTagBFP2 as a visual marker.
    Depositor
    George Eisenhoffer
    Publication
    Yen et al Dev Dyn. 2020 Nov;249(11):1394-1403. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PhyBCreC, PIF6CreN, and mTagBFP2
    Use
    Cre/Lox and Synthetic Biology; Zebrafish tol2 tra…
    Tags
    mTagBFP2
    Expression
    Mammalian
    Promoter
    Ubi
    Available since
    Jan. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    131783-plasmid-map-sequence-id-268078

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  10. pcDNA3.1-hRARα.hRXRα

    Plasmid
    #135411
    Purpose
    Human Retinoic Acid Receptor-alpha and Human Retinoid X Receptor-alpha in pcDNA3.1(+) plasmid expression vector
    Depositor
    Catharine Ross
    Publication
    Zolfaghari et al Anal Biochem. 2019 Jul 15;577:98-109. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Human retinoic acid receptor-alpha
    Human retinoid X receptor-alpha
    Expression
    Mammalian
    Promoter
    CMV and SV40
    Available since
    Jan. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    135411-plasmid-map-sequence-id-388631

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  12. pPV-EF1a-2xNLS-Cse2-Cas6-Cas3-iCA

    Plasmid
    #134923
    Purpose
    Components for genome editing in mammalian cells with pPV-EF1a-2xNLS-Cas7-Cas5-Cse1-iCA and pBS-U6-crRNA-targeted. This plasmid was optimized for iPS cells and coexpresses mCherry protein.
    Depositor
    Tomoji Mashimo
    Publication
    Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cascade factors (Cse2(Cas11), Cas3, Cas6) with bpNLS
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    134923-plasmid-map-sequence-id-267593

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  13. pTRE3G-Bi-β-chain

    Plasmid
    #133731
    Purpose
    Expresses the gePSI-β-chain and ZsGreen1 under control of the inducible bi-directional TRE3G promoter.
    Depositor
    Erin Schuman
    Publication
    Heumuller et al Nat Methods. 2019 Jul 15. pii: 10.1038/s
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    b-Chain_gePSI
    ZsGreen1
    Expression
    Mammalian
    Mutation
    Only one part of the gePSI-system (startcodon + a…
    Promoter
    pTRE3G-Bi
    Available since
    Dec. 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    133731-plasmid-map-sequence-id-266117

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  14. phGR-SB

    Plasmid
    #133409
    Purpose
    overexpression of a human glucocorticoid receptor in mammalian cells
    Depositor
    Andrew Cato
    Publication
    Heck et al EMBO J. 1994 Sep 1;13(17):4087-95.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NR3C1 (NR3C1 Human)
    Expression
    Mammalian
    Mutation
    wt
    Available since
    Dec. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    133409-plasmid-map-sequence-id-265163

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  17. miniABEmax (pRZ900)

    Plasmid
    #131311
    Purpose
    CMV promoter expression plasmid for bpNLS-TadA7.10-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS (ABEmax with truncation of WT TadA domain).
    Depositor
    Keith Joung
    Publication
    Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    bpNLS-TadA7.10-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    131311-plasmid-map-sequence-id-254652

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  19. pDONR_P2R-P3_R2-miniTurbo-NES-mVenus-STOP-L3

    Plasmid
    #127355
    Purpose
    gateway entry vector for making C-terminal miniTurbo-mVenus fusion with non-nuclear proteins
    Depositor
    Dominique Bergmann
    Publication
    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    miniTurbo (BirA mutant)
    Use
    Gateway Cloning
    Tags
    GS linker, NES, V5, and mVenus
    Mutation
    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …
    Promoter
    no promoter
    Available since
    Sept. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    127355-plasmid-map-sequence-id-246258

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Showing: 9201 - 9220 of 27467 results