27,466 results
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Plasmid#206957PurposeFluorescent reporter of the interaction between SARS-CoV-2 spike and human ACE2DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNATagscSURF, mCherry, and nSURFExpressionMammalianMutation17-615 and RBD (319-541)PromoterCMVAvailable sinceSept. 12, 2023AvailabilityAcademic Institutions and Nonprofits only
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pcDNA3.1-TEK-V5/HIS
Plasmid#205611PurposeExpression of human TEK receptor tyrosine kinase in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTEK receptor tyrosine kinase (TEK Human)
TagsV5-HisExpressionMammalianPromoterCMVAvailable sinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG1247 H2B-ECFP-FIREmate
Plasmid#205171PurposeExpresses H2B-ECFP-FIREmate in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertH2B-ECFP-FIREmate
ExpressionMammalianAvailable sinceSept. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMSSM24: pCMV-SpuFz1
Plasmid#205260PurposeHuman expression vector for human codon optimized SpuFz1DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSpuFz1
ExpressionMammalianAvailable sinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
EGFR-mApple
Plasmid#203773PurposeNumber and brightness analysis of EGFR oligomerizationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertEGFR (EGFR Human)
TagsmAppleExpressionMammalianAvailable sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHiUGE TurboID-HA donor (all-in-one) ORF0
Plasmid#200383PurposeTagging endogenously expressed proteins with TurboID-HA, C-terminalDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTurboID (ref: PMID: 30125270, Addgene plasmid # 107169)
ExpressionMammalianMutationNAAvailable sinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
A*11:01:01
Plasmid#203281PurposeOverexpress HLA alleleDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertA*11:01:01 (HLA-A Human)
ExpressionMammalianAvailable sinceJuly 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDD428
Plasmid#202081PurposeCas9/sgRNA plasmid targeting the C-terminus of Myh9DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertpEF1a-mCherry-Cas9 + Myh9 CT sgRNA (Myh9 S. pyogenes (Cas9))
UseCRISPRPromoterEF1aAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-PDGFRB-V5/HIS
Plasmid#201988Purposeexpression of human PDGFRB receptor tyrosine kinase in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPDGFRB receptor tyrosine kinase (PDGFRB Human)
TagsV5, 6xHisExpressionMammalianPromoterCMVAvailable sinceJune 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1-ADGRL3-Flag
Plasmid#201218PurposepCDNA3.1 with kozak (GCC), ADGRL3 and C-terminal FlagDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertADGRL3 (Adgrl3 Mouse)
TagsFlagExpressionMammalianPromoterCMVAvailable sinceMay 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
mBFP-APP-mGFP
Plasmid#196694PurposeExpresses APP695 with two fluorescent proteins at the ends to follow processing in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertAmyloid Precursor Protein 695 isoform (APP Human)
TagsTagGFP2 and mtagBFP2ExpressionMammalianPromoterCMVAvailable sinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
Membrane mNeonGreen
Plasmid#198057PurposePlasma membrane localizing mNeonGreen using Human LCK proto-oncogene sequenceDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLCK membrane mNeonGreen (LCK A.victoria, Human)
UseSynthetic Biology; Sea urchin, mammal, zebrafishTagsmNeonGreenMutationTandem repeat of N-terminal LCK membrane targetin…Promotersp6Available sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-Pur-dCas9-KRAB-MeCP2_hU6-SapI
Plasmid#196078Purpose(Empty Backbone) Constitutive CRISPRi vector conferring puromycin resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsdCas9-KRAB-MeCP2 repressor
hU6 promoter and gRNA scaffold
ExpressionMammalianMutationCatalytically dead mutant of the Cas9 endonucleas…Available sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
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pLexM-SLC7A11_GFP
Plasmid#197995PurposeExpresses SLC7A11 with an N-terminal FLAG tag and C-terminal TEV-GFP-His8 tag in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSLC7A11 (SLC7A11 Human)
TagsFLAG tag and TEV-GFP-His8ExpressionMammalianPromoterCMVAvailable sinceMarch 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
EB3-mNeonGreen
Plasmid#191761PurposeExpression of mNeonGreen fused to EB3DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertEB3-mNeonGreen (MAPRE3 Human)
TagsmNeonGreenExpressionBacterial and MammalianPromoterCMVAvailable sinceFeb. 8, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PB-TRE-dCas9-VPR-pA-3xsgRNA-EF1a-Tet.O-T2A-PuroR-polyA
Plasmid#166693PurposeExpress dCas9-VPR in a doxycyclin-inducable system and 3 sgRNAs targeting the murine Cnga1 promoter. Can be stably integrated into the genome via the PiggyBac Transposon system.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsdCas9-VPR
3x Cnga1 promoter-targeting sgRNAs
UseCRISPRExpressionMammalianPromoterTRE and U6Available sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
LYSO-LRRK2
Plasmid#193582PurposeExpresses human full-length LRRK2 with a lysosomal targeting sequence from LAMTOR1 (1-39aa) and 3xFLAG on the N-terminusDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLeucine-rich repeat kinase 2 (LRRK2 Human)
TagsLAMTOR1(1-39aa) and 3xFLAGExpressionMammalianPromoterCMVAvailable sinceJan. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAK_DR30_CasRx_GFP
Plasmid#134843PurposeAll in one overexpression of CasRx and guideRNADepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRfxCas13d
UseCRISPRTagsT2A EGFPExpressionMammalianPromoterCAGAvailable sinceJan. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
CABE-T3.155
Plasmid#193270PurposeExpression of CABE-T3.155 base editor in mammalian cells, CMV promoterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCABE-T3.155
ExpressionMammalianMutationD10A (Cas9), TadA mutations withinPromoterCMVAvailable sinceJan. 9, 2023AvailabilityAcademic Institutions and Nonprofits only