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Showing: 10061 - 10080 of 27466 results
  1. pCDNA3.1-spikeRBD::∆ACE2 SURF

    Plasmid
    #206957
    Purpose
    Fluorescent reporter of the interaction between SARS-CoV-2 spike and human ACE2
    Depositor
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    sars GU280_gp02, spike glycoprotein (S SARS-CoV-2)
    ACE2 (ACE2 Human)
    Tags
    cSURF, mCherry, and nSURF
    Expression
    Mammalian
    Mutation
    17-615 and RBD (319-541)
    Promoter
    CMV
    Available since
    Sept. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
  2. pDD428

    Plasmid
    #202081
    Purpose
    Cas9/sgRNA plasmid targeting the C-terminus of Myh9
    Depositor
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    pEF1a-mCherry-Cas9 + Myh9 CT sgRNA (Myh9 S. pyogenes (Cas9))
    Use
    CRISPR
    Promoter
    EF1a
    Available since
    July 5, 2023
    Availability
    Academic Institutions and Nonprofits only
  3. mBFP-APP-mGFP

    Plasmid
    #196694
    Purpose
    Expresses APP695 with two fluorescent proteins at the ends to follow processing in mammalian cells
    Depositor
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Amyloid Precursor Protein 695 isoform (APP Human)
    Tags
    TagGFP2 and mtagBFP2
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 26, 2023
    Availability
    Academic Institutions and Nonprofits only
  4. Membrane mNeonGreen

    Plasmid
    #198057
    Purpose
    Plasma membrane localizing mNeonGreen using Human LCK proto-oncogene sequence
    Depositor
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    LCK membrane mNeonGreen (LCK A.victoria, Human)
    Use
    Synthetic Biology; Sea urchin, mammal, zebrafish
    Tags
    mNeonGreen
    Mutation
    Tandem repeat of N-terminal LCK membrane targetin…
    Promoter
    sp6
    Available since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
  5. pSBbi-Pur-dCas9-KRAB-MeCP2_hU6-SapI

    Plasmid
    #196078
    Purpose
    (Empty Backbone) Constitutive CRISPRi vector conferring puromycin resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.
    Depositor
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    dCas9-KRAB-MeCP2 repressor
    hU6 promoter and gRNA scaffold
    Expression
    Mammalian
    Mutation
    Catalytically dead mutant of the Cas9 endonucleas…
    Available since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
  6. PB-TRE-dCas9-VPR-pA-3xsgRNA-EF1a-Tet.O-T2A-PuroR-polyA

    Plasmid
    #166693
    Purpose
    Express dCas9-VPR in a doxycyclin-inducable system and 3 sgRNAs targeting the murine Cnga1 promoter. Can be stably integrated into the genome via the PiggyBac Transposon system.
    Depositor
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    dCas9-VPR
    3x Cnga1 promoter-targeting sgRNAs
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    TRE and U6
    Available since
    Feb. 1, 2023
    Availability
    Academic Institutions and Nonprofits only
  7. LYSO-LRRK2

    Plasmid
    #193582
    Purpose
    Expresses human full-length LRRK2 with a lysosomal targeting sequence from LAMTOR1 (1-39aa) and 3xFLAG on the N-terminus
    Depositor
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Leucine-rich repeat kinase 2 (LRRK2 Human)
    Tags
    LAMTOR1(1-39aa) and 3xFLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Jan. 17, 2023
    Availability
    Academic Institutions and Nonprofits only
  8. pAK_DR30_CasRx_GFP

    Plasmid
    #134843
    Purpose
    All in one overexpression of CasRx and guideRNA
    Depositor
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    RfxCas13d
    Use
    CRISPR
    Tags
    T2A EGFP
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Jan. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
  9. CABE-T3.155

    Plasmid
    #193270
    Purpose
    Expression of CABE-T3.155 base editor in mammalian cells, CMV promoter
    Depositor
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CABE-T3.155
    Expression
    Mammalian
    Mutation
    D10A (Cas9), TadA mutations within
    Promoter
    CMV
    Available since
    Jan. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
Showing: 10061 - 10080 of 27466 results