27,466 results
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Plasmid#194060PurposeMammalian expression of evoCjCas9 ABE8eDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertevoCjCas9-ABE8e
UseCRISPRExpressionMammalianAvailable sinceSept. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFH2.94_PB-TRE3GS-Gag-MCP-T2A-eGFP-PGK-Tet3G-T2A-Blast
Plasmid#205540PurposeExpress HIV Gag-MCP-T2A-eGFP in doxycycline-inducible manner, and constitutively express Tet3G transactivator and blasticidin resistance markerDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGag-MCP
UsePiggybacExpressionMammalianMutationWTPromoterTRE3GSAvailable sinceSept. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
hKCNQ3(WT).ires.mCherry-pcDNA3
Plasmid#204362PurposeHeterologous expression in mammalian cell lines or Xenopus oocyte (T7 RNA pol)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertKCNQ3 (KCNQ3 Human)
ExpressionMammalianPromoterCMV/T7 RNApolAvailable sinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_dCas7-11
Plasmid#197605PurposeExpresses inactive Cas7-11 in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertdCas7-11
ExpressionMammalianAvailable sinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
NbV5-HiBiT
Plasmid#201489PurposeNanoBit detection of V5-tagged proteinDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNbV5-HiBiT
TagsHiBitExpressionMammalianPromoterCMVAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHiUGE TurboID-HA donor (all-in-one) ORF1
Plasmid#200384PurposeTagging endogenously expressed proteins with TurboID-HA, C-terminalDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTurboID (ref: PMID: 30125270, Addgene plasmid # 107169)
ExpressionMammalianMutationNAAvailable sinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
PAmCherry1-C1-P4Mx2
Plasmid#202681PurposePhotoactivatable lipid biosensorDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPAmCherry1: L. pneumophila SidM(546-647):SidM(546-647) (sidM Legionella pneumophila)
ExpressionMammalianAvailable sinceJune 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-FLT3-V5/HIS
Plasmid#201985Purposeexpression of human FLT3 receptor tyrosine kinase in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertFLT3 receptor tyrosine kinase (FLT3 Human)
TagsV5, 6xHisExpressionMammalianPromoterCMVAvailable sinceJune 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
mtsCOXVIIIx2-B-gTEMP/pcDNA3
Plasmid#199806PurposeFluorescent temperature indicator B-gTEMP fused with mitochondrial targeting sequenceDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertB-gTEMP
TagsmtsCOXVIIIx2ExpressionMammalianAvailable sinceJune 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-DDR1-V5/HIS
Plasmid#201100Purposeexpression of human DDR1 receptor tyrosine kinase in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthuman DDR1 receptor tyrosine kinase, full length, wildtype (DDR1 Human)
TagsV5/HisExpressionMammalianPromoterCMVAvailable sinceMay 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
PGK 4xMito-mEmerald
Plasmid#200430PurposeMammalian expression of fluorescently-tagged marker for mitochondriaDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsert4x cytochrome c oxidase subunit VIII mitochondrial targeting sequence
TagsmEmeraldExpressionMammalianMutation4x mito-targeting sequencePromoterPGKAvailable sinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AW01_pU.CAG.eSpCas9(1.1).rBGpA
Plasmid#199255PurposeExpression construct encoding the high specificity eSpCas9(1.1) nucleaseDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHigh specificity eSpCas9(1.1) nuclease
UseCRISPRTags3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLSExpressionMammalianMutationK848A K1003A R1060APromoterCAG promoterAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
mBFP-APPP1-mGFP
Plasmid#196695PurposeExpresses mBFP-APP-TagGFP2, with substitution at position 612 that prevents alpha-secretase activityDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertAmyloid Precursor Protein 695 isoform (APP Human)
TagsTagGFP2 and mtagBFP2ExpressionMammalianMutationLys to Val substitution at position 612PromoterCMVAvailable sinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mCherry-APPP1-mGFP
Plasmid#196705PurposeExpresses mCherry-APP-TagGFP2, with substitution at position 612 that prevents alpha-secretase activityDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertAmyloid Precursor Protein 695 isoform (APP Human)
TagsTagGFP2 and mCherryExpressionMammalianMutationLys to Val substitution at position 612Available sinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTET2-RlucCP-3MS2
Plasmid#198354PurposePart of Renilla reporter system, encodes Renilla luciferase with MS2 stem loops in the 3'UTRDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRenilla Luciferase
UseLuciferaseExpressionMammalianPromoterpTET2Available sinceApril 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
Membrane EGFP
Plasmid#198056PurposePlasma membrane localizing EGFP using Human LCK proto-oncogene sequenceDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLCK membrane EGFP (LCK A. victoria, Human, Synthetic)
UseSea urchin, mammal, zebrafishTagsEGFPMutationTandem repeat of N-terminal LCK membrane targetin…Promotersp6Available sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pPB-TetON-mEGFP-HMGB1-WT
Plasmid#194561PurposeDox-inducible Mammalian Expression of mEGFP-HMGB1 WT IDR Fusion ProteinDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHMGB1 (HMGB1 Human)
TagsmEGFPExpressionMammalianAvailable sinceFeb. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
GFP-myrlysin/BORCS5
Plasmid#194332PurposeExpresses myrlysin in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertBORCS5 (BORCS5 Human)
TagsGFPExpressionMammalianPromoterCMVAvailable sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSBtet rtTA G72V SE Δsplice GP Luc
Plasmid#194328PurposeTetracycline inducible expression of Luciferase enzyme in mammalian cells with inclusion of rtTA G72V mutation, splice site and high sensitivity mutations for increased promotor tightnessDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLuciferase
UseLuciferaseExpressionMammalianMutationTet-responsive promotor related mutations: G72V i…PromoterTight tet-responsive promotorAvailable sinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
iBE4max YE1 NG CLYBL
Plasmid#174570Purposedoxycycline inducible BE4max YE1 NG expression for human CLYBL locus knock-inDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertBE3
ExpressionMammalianPromotertet ONAvailable sinceJan. 19, 2023AvailabilityAcademic Institutions and Nonprofits only