Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

Applied Filters

Clear All Filters
50 µg Transfection-Ready DNA Clear filter

Applied Filters

Clear All Filters
50 µg Transfection-Ready DNA Clear filter

30,881 results

Showing: 6421 - 6440 of 30881 results

  4. OpenCRISPR-1 sgRNA-12nt stem

    Plasmid
    #221567
    Purpose
    Express OpenCRISPR-1 sgRNA (12nt stem loop) in human cells using a U6 promoter. Plasmid also encodes a CMV-driven GFP reporter.
    Depositor
    Ali Madani
    Publication
    Ruffolo et al Nature. 2025 Jul 30. doi: 10.1038/s41586
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    OpenCRISPR-1 sgRNA 12nt stem loop
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Aug. 28, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    221567-plasmid-map-sequence-id-437907

    Enlarge

  7. pEF1a-PEmax-P2A-GFP

    Plasmid
    #202000
    Purpose
    Based on the pCMV-PEmax-P2A-GFP, we replaced the CMP promoter with the EF1a promoter for expression in iPSC.
    Depositor
    Daniel Geschwind
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PEmax-P2A-GFP
    Use
    CRISPR and Synthetic Biology
    Tags
    GFP
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    202000-plasmid-map-sequence-id-395506

    Enlarge

  8. mCherry-APP-mGFP

    Plasmid
    #196704
    Purpose
    Expresses APP695 with two fluorescent proteins at the ends to follow processing in mammalian cells
    Depositor
    Martino Calamai
    Publication
    Capitini et al iScience. 2023 Apr 11;26(5):106611. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Amyloid Precursor Protein 695 isoform (APP Human)
    Tags
    TagGFP2 and mEGFP
    Expression
    Mammalian
    Available since
    April 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    196704-plasmid-map-sequence-id-386429

    Enlarge

  13. ER-RpHLuorin2

    Plasmid
    #171717
    Purpose
    Luminal ER expression of ratiometric pHLuorin2
    Depositor
    Geert van den Bogaart
    Publication
    Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Ratiometric pHLuorin2 targeted to ER
    Tags
    KDEL ER-retention signal and Signal sequence of h…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    March 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    171717-plasmid-map-sequence-id-355806

    Enlarge

  14. pFX-Tom20-ｍCherry

    Plasmid
    #174188
    Purpose
    Express mCherry with mitochondria-targeting sequence in mammalian cells
    Depositor
    Yusuke Ohba
    Publication
    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mCherry with mitchondria-targeting sequence
    Tags
    the mitochondria-targeting sequence of TOM20
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    174188-plasmid-map-sequence-id-354791

    Enlarge

  18. Epac-S-H188

    Plasmid
    #170349
    Purpose
    mT2Del_EPACdDEPCD_tdcp173Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.
    Depositor
    Kees Jalink
    Publication
    Klarenbeek et al PLoS One. 2015 Apr 14;10(4):e0122513. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mT2Del_EPACdDEPCD_tdcp173Ven(ST) (RAPGEF3 Human)
    Expression
    Mammalian
    Mutation
    Deletion of DEP domain, Catalytically dead T781A …
    Promoter
    CMV
    Available since
    Oct. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    170349-plasmid-map-sequence-id-344647

    Enlarge

  20. slc1a3b:myrGCaMP6s-P2A-H2AmCherry

    Plasmid
    #170209
    Purpose
    Drives myrGCaMP6s-P2A-H2AmCherry expression with the slc1a3b promoter in astrocytes. The myrGCaMP6s calcium indicator expressed in the membrane and mCherry labels the nucleus
    Depositor
    Kelly Monk
    Publication
    Chen et al Nat Neurosci. 2020 Oct;23(10):1297-1306.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    slc1a3b (slc1a3b Zebrafish)
    Use
    Zebrafish
    Tags
    myrGCaMP6s-P2A-H2AmCherry
    Promoter
    slc1a3b
    Available since
    June 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    170209-plasmid-map-sequence-id-333268

    Enlarge

Showing: 6421 - 6440 of 30881 results