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30,881 results

Showing: 7581 - 7600 of 30881 results

  1. pSB-CMV-MCS-Puro cPLA2-mK2-P2A-EGFP-LaminB1

    Plasmid
    #162566
    Purpose
    Used for the dual color labeling of cPLA2 and LaminB1 expression.
    Depositor
    Philipp Niethammer
    Publication
    Enyedi et al Cell. 2016 May 19;165(5):1160-1170. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    cytosolic phospholipase a2 (pla2g4aa Zebrafish)
    Lamin B1 (lmnb1 Zebrafish)
    Tags
    EGFP, P2A, and mKate2
    Expression
    Mammalian
    Promoter
    CMV and SV40
    Available since
    March 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    162566-plasmid-map-sequence-id-325979

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  5. Clathrin-mGreenLantern

    Plasmid
    #164462
    Purpose
    Localization of mGreenLantern fluorescent protein to clathrin
    Depositor
    Gregory Petsko
    Publication
    Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mGreenLantern
    Tags
    Clathrin
    Expression
    Mammalian
    Mutation
    Clover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…
    Promoter
    CMV
    Available since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    164462-plasmid-map-sequence-id-322670

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  8. AAVS1-pur-CAG-Bi-DREADD

    Plasmid
    #159457
    Purpose
    AAVS1 targeting donor plasmid with Bi-DREADD expression cassette (hM3Dq-mCherry-P2A-HA-KORD) and puromycin selection gene
    Depositor
    Yuejun Chen
    Publication
    Xiong et al Cell Stem Cell. 2020 Sep 16. pii: S1934-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Bi-DREADD
    Expression
    Mammalian
    Mutation
    fusion protein of hM3Dq-mCherry and HA-KORD seper…
    Promoter
    CAG
    Available since
    Dec. 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    159457-plasmid-map-sequence-id-319218

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  10. Cas9-sgRNA-A+B

    Plasmid
    #149370
    Purpose
    Expresses SpCas9 and two sgRNA targeting the lenti-CDDR reporter at two distal sites
    Depositor
    Tarek Abbas
    Publication
    Eki et al Nucleic Acids Res. 2020 Oct 17. pii: 592
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Cas9
    sgRNA-A
    sgRNA-B
    Puromycin resistance
    Use
    CRISPR
    Tags
    3xFLAG, Nucleoplasmin NLS, and SV40 NLS
    Expression
    Mammalian
    Promoter
    CBh, PGK, and U6
    Available since
    Oct. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    149370-plasmid-map-sequence-id-315474

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  11. BFP Homology Donor

    Plasmid
    #149372
    Purpose
    Promoter-less BFP cDNA with two homology arms to the lenti-CDDR reporter for HDR detection
    Depositor
    Tarek Abbas
    Publication
    Eki et al Nucleic Acids Res. 2020 Oct 17. pii: 592
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CDDR BFP Homology Donor
    Expression
    Mammalian
    Available since
    Oct. 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    149372-plasmid-map-sequence-id-313974

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  12. hHS1-M7A

    Plasmid
    #159170
    Purpose
    Human codon-optimized cytosolic labile heme reporter
    Depositor
    Amit Reddi
    Publication
    Hanna et al Proc Natl Acad Sci U S A. 2016 Jul 5;113
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hHS1-M7A
    Expression
    Mammalian
    Mutation
    Mutated Met 7 of the cyt b562 module of hHS1 to A…
    Promoter
    CMV
    Available since
    Sept. 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    159170-plasmid-map-sequence-id-311541

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  13. 6xHis-TagRFP-T_A1aY1

    Plasmid
    #158754
    Purpose
    Expression of 6x His tagged TagRFP-T_A1aY1 in mammalian cells for detection of tyrosinated microtubules.
    Depositor
    Minhajuddin Sirajuddin
    Publication
    Kesarwani et al J Cell Biol. 2020 Oct 5;219(10). pii: 15
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    6xHistidine_TagRFP-T_A1aY1
    Tags
    6x-Histidine tag and TagRFP-T
    Expression
    Mammalian
    Promoter
    CMV Promoter
    Available since
    Sept. 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    158754-plasmid-map-sequence-id-310757

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  14. pcDNA3.1.Gα13-LgB106

    Plasmid
    #134364
    Purpose
    Nanoluc complementation assay. Expression of Gα13 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 106 and 107 of Gα13. Addition of the HA epitope at N term of Gα13.
    Depositor
    Julien Hanson
    Publication
    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Gα13-LgB106 (GNA13 Human)
    Tags
    HA
    Expression
    Bacterial and Mammalian
    Promoter
    T7
    Available since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    134364-plasmid-map-sequence-id-306388

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  15. pU6-ACTA2_gRNA1-SpCas9-T2A-GFP

    Plasmid
    #126706
    Purpose
    to express Cas9 from S. pyogenes with 2A-EGFP and sgRNA targeting human ACTA2 locus at the end of the endogenous coding sequence
    Depositor
    Darrell Kotton
    Publication
    Kwong et al Stem Cell Reports. 2019 Sep 10;13(3):499
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ACTA2_gRNA1-SpCas9-T2A-GF (ACTA2 Human)
    Use
    CRISPR
    Available since
    July 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    126706-plasmid-map-sequence-id-245754

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  17. pcDNA5/FRT/TO-GAlphai2-RLuc8

    Plasmid
    #140974
    Purpose
    Encodes a G alpha subunit (GNAI2) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Publication
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GAlphai2-Rluc8 (GNAI2 Human)
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    140974-plasmid-map-sequence-id-300657

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  18. pcDNA5/FRT/TO-GAlphaGustducin-RLuc8

    Plasmid
    #140979
    Purpose
    Encodes a G alpha subunit (GNAT3) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Publication
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GAlphaGustducin-RLuc8 (GNAT3 Human)
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140979-plasmid-map-sequence-id-300662

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Showing: 7581 - 7600 of 30881 results