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27,006 results

Showing: 8001 - 8020 of 27006 results

  1. pDRESS_mTurquoise2_spatial-linker-P2A_mScarlet-I3

    Plasmid
    #189755
    Purpose
    Produces an mTurquoise-antiFRET-mScarlet-I3 fusion protein in bacteria and in mamallian cells it produces 1:1 unfused mTurquoise2 and mScarlet-I3 FPs for ratiometric analysis
    Depositor
    Dorus Gadella
    Publication
    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mTurquoise2_spatial-linker-P2A_mScarlet-I3
    Tags
    6xHis
    Expression
    Bacterial and Mammalian
    Promoter
    CMV & Rha
    Available since
    March 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    189755-plasmid-map-sequence-id-385774

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  2. pCMV-T7-ABE8e-SpCas9-NRTH-P2A-EGFP (RMD21)

    Plasmid
    #197503
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-NRTH(D10A) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    pCMV-T7-BPNLS-ABE8e-nSpCas9-NRTH-BPNLS-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-P2A-EGFP
    Expression
    Mammalian
    Mutation
    ABE8e mutations in TadA and nSpCas9-NRTH(D10A/I32…
    Promoter
    CMV and T7
    Available since
    March 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    197503-plasmid-map-sequence-id-386604

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  6. pCXLE-Oct4-p2a-Klf4-IRES-Sox2-17

    Plasmid
    #193298
    Purpose
    episomal OKS* (expressing mouse Oct4 + Klf4 + super-Sox) for generation of integration-free iPSCs
    Depositor
    Hans Schöler
    Publication
    MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Oct4-p2a-Klf4-IRES-Sox2-17 (Sox2, Pou5f1, Klf4 Mouse)
    Expression
    Mammalian
    Mutation
    Sox2-17 is engineered highly cooperative chimeric…
    Promoter
    CAG
    Available since
    Dec. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    193298-plasmid-map-sequence-id-379909

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  7. T7-VEE-mCherry

    Plasmid
    #193355
    Purpose
    Self-replicating RNA vector expressing mCherry fluorescent protein + PuroR
    Depositor
    Hans Schöler
    Publication
    MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mCherry
    Use
    Venezuelan equine encephalitis (vee) virus rna re…
    Expression
    Mammalian
    Promoter
    T7
    Available since
    Dec. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    193355-plasmid-map-sequence-id-379856

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  11. CMV mRuby3-Synapsin1a

    Plasmid
    #187896
    Purpose
    For labeling pre-synaptic terminals
    Depositor
    Timothy Ryan
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Synapsin1a
    Tags
    mRuby3
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    187896-plasmid-map-sequence-id-394752

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  13. pCMV-T7-ABE8e-nSpCas9-VRQR-P2A-EGFP (KAC1309)

    Plasmid
    #185913
    Purpose
    pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpCas9-VRQR(D10A/D1135V/G1218R/R1335Q/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized ABE8e-nSpCas9-VRQR-P2A-EGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9-VRQR=D10A/D1135V/G1218R/R1335Q/T1337R
    Promoter
    CMV and T7
    Available since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    185913-plasmid-map-sequence-id-367365

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  14. pcDNA3_mScarlet-LAMP1

    Plasmid
    #185138
    Purpose
    Expresses mScarlet in mammalian lysosomes for pH quantification with fluorescence lifetime
    Depositor
    Andrew York
    Publication
    Lazzari-Dean et al (2022), doi: 10.5281/zenodo.6363342
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Lysosome-associated membrane glycoprotein 1 (Lamp1 Synthetic, Rat)
    Tags
    mScarlet
    Expression
    Mammalian
    Mutation
    deleted amino acids 1-21 of LAMP1
    Promoter
    CMV
    Available since
    July 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    185138-plasmid-map-sequence-id-366475

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  18. pICE-EGFP-FLAG-PSMD2

    Plasmid
    #161917
    Purpose
    Expresses human PSMD2 with a N-terminal GFP-FLAG tag. Confers Puromycin resistance. Inducible in T-REx cells.
    Depositor
    Sébastien Britton
    Publication
    Demange et al Elife. 2022 May 10;11. pii: 73913. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    26S proteasome non-ATPase regulatory subunit 2 (PSMD2 Human)
    Tags
    GFP-FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    May 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    161917-plasmid-map-sequence-id-318837

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  19. XLone-PE2

    Plasmid
    #136463
    Purpose
    Expresses prime editing 2 (PE2) enzyme upon doxycycline treatment
    Depositor
    Xiaojun Lian
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PE2
    Expression
    Mammalian
    Promoter
    TRE3GS
    Available since
    May 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    136463-plasmid-map-sequence-id-272540

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Showing: 8001 - 8020 of 27006 results