30,881 results
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Plasmid#182577PurposeExpresses LifeAct-GFP targeted to the ER via the CytB5RR tail anchor sequenceDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLifeAct-GFP-cytoB5RR
TagsLifeAct and cytoB5RRExpressionMammalianAvailable sinceApril 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCS2 LRP6-eGFP
Plasmid#180143Purposeto make movies of LRP6 vesiclesDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHuman LRP6 (LRP6 Human)
ExpressionMammalianPromoterSP6Available sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAGGS_cAMPFIRE-M
Plasmid#182284PurposeMammalian expression of the cAMP sensor cAMPFIRE-M under a CAG promotor w/ WPRE translation enhancer.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertcAMPFIRE-M (cAMP sensor)
ExpressionMammalianAvailable sinceMarch 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDAS12395_PEAR-GFP_2.0-preedited
Plasmid#177185PurposePEAR-GFP3 control plasmid with split EGFP and intact 5' splice siteDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertEGFP split with an intron between amino acids 95-96
UseCRISPRExpressionMammalianPromoterCMVAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
rASAP
Plasmid#178205PurposeRatiometric version of the genetically encoded voltage indicator ASAP2s-I67T-Q397R.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertrASAP
ExpressionMammalianAvailable sinceFeb. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-hPPP3CA-FLAG
Plasmid#179134PurposeExpression vector of human protein phosphatase 3, catalytic subunit, alpha isoform (PPP3CA) tagged with FLAG at C-terminus, CAG promoter, rabbit globin poly(A) signal.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertprotein phosphatase 3, catalytic subunit, alpha isoform (PPP3CA Human)
TagsFLAGExpressionMammalianAvailable sinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB-PE
Plasmid#162796PurposeAll-in-one prime editor piggyBac transposonDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSpCas9_ H840A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
UseCRISPR and Synthetic Biology; Piggybac transposonTagsSV40 NLSExpressionMammalianPromoterCAGAvailable sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
dCas9-10xSunTag-P2A-tagBFP
Plasmid#174141PurposeExpresses the dCas9-10xSunTag-tagBFP fusion protein for the recruitment of scFv(GCN4)-fused effector domains. A shorter version of the Addgene 60903 plasmid.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertdCas9, 10xSunTag, tagBFP
TagsHA and tagBFPExpressionMammalianPromoterSV40Available sinceOct. 22, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
dCas9-DNMT3a-DNMT3l-3xFLAG-tagBFP
Plasmid#154139PurposeExpresses the dCas9-DNMT3a-DNMT3l-3xFLAG-tagBFP fusion protein for targeted DNA methylation.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTags3x FLAG and tagBFPExpressionMammalianPromoterCMVAvailable sinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Frankenbody-FLAG-SNAP
Plasmid#175295PurposeTrack mature FLAG tagged proteins in live cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertanti-FLAG frankenbody fused with SNAP-tag
TagsSNAP-tagExpressionMammalianAvailable sinceOct. 19, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAB1676 REPAIR.t1
Plasmid#176319PurposeCMV-HIVNES-GS-dCas13bt1- (GGS)2-huADAR2dd(E488Q) (REPAIR.t1 expression)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsUseCRISPRTagsHIV NESExpressionMammalianMutationE488Q, only the deaminase domain (aa 276-702 are …PromoterCMVAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-mitoT
Plasmid#174519PurposeEngineered intermembrane bridge to link the inner and outer mitochondrial membranes in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMitoT synthetic tether
Tags6xHis and eGFPExpressionMammalianPromoterCMVAvailable sinceSept. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX330 _CRISPIE donor B9
Plasmid#172850PurposeDRS-2 sgRNA expression under a U6 promotor and CRISPIE donor B9 (Zhong et al, eLife 2021), mEGFP translational phase (2-2), excised by DRS-1 or DRS-2 sgRNA (with SpCas9).DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertDRS-2 sgRNA and CRISPIE designer exon (phase 2-2) encoding mEGFP
UseCRISPRExpressionMammalianAvailable sinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CMV-dCas13X.1-RESCUE-S-SV40pA_U6-BbsI-DR_CMV-mCherry-P2A-Puro-BGHpA
Plasmid#171384PurposeExpression vector for encoding a human codon-optimized dCas13X.1-RESCUE-S driven by CMV promoter,mCherry driven by CMV promoter and U6-driven crRNAs cloning site.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthumanized dCas13X.1-RESCUE-S
ExpressionMammalianPromoterCMV, U6Available sinceJuly 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
HA-AR-LBD
Plasmid#171239PurposeMammalian expression of 1xHA-tagged human androgen receptor trancate: AR Ligand-bindind domain (LBD)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsert1xHA-tagged human androgen receptor trancate:Ligand-binding domain (AR Human)
TagsHAExpressionBacterial and MammalianMutationAR truncatePromoterCMVAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
CIp10 - γmGFP (γmGFP based)
Plasmid#163119PurposeExpresses in Candida albicansDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertgamma mGFP
UseCandida albicans expressionAvailable sinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1-GGamma3-GFP2
Plasmid#166775PurposeEncodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGGamma3-GFP2 (GNG3 Human, Synthetic)
ExpressionMammalianMutationContains an N-terminal GFP2 and GSAG linkerPromoterCMVAvailable sinceMay 25, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCG-TPMV Fd32
Plasmid#163964PurposeExpression of TPMV F glycoprotein in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTPMV F glycoprotein
ExpressionMammalianMutationLacking 32 amino acids at C-terminusAvailable sinceMay 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Beta2
Plasmid#166772PurposeEncodes a Gbeta subunit as a "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGNB2 (GNB2 Human)
ExpressionMammalianPromoterCMVAvailable sinceApril 22, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1+(Zeo)-FLAG-muGFP
Plasmid#166726PurposeExpresses FLAG-tagged monomeric ultrastable GFP (muGFP) in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmuGFP
TagsFLAGExpressionMammalianPromoterCMVAvailable sinceApril 12, 2021AvailabilityAcademic Institutions and Nonprofits only