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30,881 results

Showing: 8901 - 8920 of 30881 results

  1. pJ609-dCas9-KR-puro

    Plasmid
    #158478
    Purpose
    Express nuclease dead Cas9 fused to KillerRed chromophore in mammalian cells
    Depositor
    Brendan Price
    Publication
    House et al PLoS One. 2020 Dec 17;15(12):e0237759. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9-KR
    Use
    CRISPR
    Tags
    KillerRed
    Expression
    Mammalian
    Mutation
    Cas9m4 (D10A+D839A+H840A+N863A)
    Promoter
    CMV
    Available since
    Nov. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    158478-plasmid-map-sequence-id-317143

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  3. pCD5-PR8-TEV-sfGFP-twinStrep

    Plasmid
    #157933
    Purpose
    Expresses influenza A virus trimeric hemagglutinin fused to a sfGFP
    Depositor
    Robert de Vries
    Publication
    Nemanichvili et al J Mol Biol. 2019 Feb 15;431(4):842-856.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    A/PuertoRico/8/34 hemagglutinin (HA Synthetic)
    Tags
    sfGFP & TwinStrep
    Expression
    Mammalian
    Mutation
    E190D
    Promoter
    CMV
    Available since
    Oct. 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    157933-plasmid-map-sequence-id-315089

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  4. pEGFP-NUP188

    Plasmid
    #159893
    Purpose
    Expresses NUP188 in mammalian cells
    Depositor
    Kozo Tanaka
    Publication
    Itoh et al Cancer Sci. 2013 Jul;104(7):871-9. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NUP188 (NUP188 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    To avoid NotI site, first three amino acids are d…
    Promoter
    CMV
    Available since
    Oct. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    159893-plasmid-map-sequence-id-313971

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  5. DD-DamV133A-tdTomato-LMNB1

    Plasmid
    #159604
    Purpose
    Shield-1 inducible transient mammalian expression of Dam-tdTomato-LMNB1 for DamID, containing the V133A mutant of Dam and a fused tdTomato to monitor fusion protein localization and expression levels.
    Depositor
    Aaron Streets
    Publication
    Altemose et al Cell Syst. 2020 Oct 21;11(4):354-366.e9.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    DD-DamV133A-tdTomato-LMNB1
    Expression
    Mammalian
    Mutation
    Dam V133A mutation
    Promoter
    CMV
    Available since
    Sept. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    159604-plasmid-map-sequence-id-311954

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  6. DD-DamV133A-IRES2-mCherry

    Plasmid
    #159600
    Purpose
    Shield-1 inducible transient mammalian expression of Dam alone for DamID controls, containing the V133A mutant of Dam.
    Depositor
    Aaron Streets
    Publication
    Altemose et al Cell Syst. 2020 Oct 21;11(4):354-366.e9.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    DD-DamV133A-IRES2-mCherry
    Expression
    Mammalian
    Mutation
    Dam V133A mutation
    Promoter
    CMV
    Available since
    Sept. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    159600-plasmid-map-sequence-id-311948

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  7. elavl3:SomaGCaMP7f

    Plasmid
    #158760
    Purpose
    Cell body-targeted GCaMP7f, under HuC promoter: GCaMP7f followed by a linker, and the EE-RR coiled coil motif (GCaMP7f-27-EE-RR). As bright as conventional GCaMP7f.
    Depositor
    Edward Boyden
    Publication
    Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SomaGCaMP7
    Use
    Synthetic Biology
    Promoter
    HuC promoter
    Available since
    Sept. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    158760-plasmid-map-sequence-id-310667

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  9. Venus1-WTSTAT3

    Plasmid
    #123164
    Purpose
    Expresses wild type STAT3 fused to the 1-157 fragment of Venus in mammalian cells
    Depositor
    Federico Herrera
    Publication
    Letra-Vilela et al FASEB Bioadv. 2020 Jan 27;2(2):116-125.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    STAT3 tagged with Venus1 (aa 1-157) (STAT3 Human)
    Tags
    Venus1 (aa 1-158) for bimolecular fluorescence co…
    Expression
    Mammalian
    Mutation
    none
    Promoter
    pCMV
    Available since
    Aug. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    123164-plasmid-map-sequence-id-238631

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  10. pBBR_Cas9_sp1

    Plasmid
    #154851
    Purpose
    Expresses SpCas9 and sgRNA that targets the upp gene in Rhodobacter sphaeroides genome
    Depositor
    John van der Oost
    Publication
    Mougiakos et al Microb Cell Fact. 2019 Nov 25;18(1):204.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Spcas9 gene
    sgRNA scaffold
    Use
    CRISPR
    Tags
    6xHis
    Mutation
    Codon harmonized variant for expression in Rhodob…
    Available since
    Aug. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    154851-plasmid-map-sequence-id-309592

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  16. miniCGBE1-NG (pBM1182)

    Plasmid
    #140257
    Purpose
    CMV promoter expression plasmid for rAPOBEC1(R33A)-nCas9_NG-P2A-EGFP
    Depositor
    Keith Joung
    Publication
    Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    BE4max(R33A)_NG-ΔUGI
    Expression
    Mammalian
    Mutation
    R33A in rAPOBEC1, NG mutations in SpCas9, D10A in…
    Promoter
    CMV
    Available since
    Aug. 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    140257-plasmid-map-sequence-id-291773

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  17. AAVS1-TRE-SHH

    Plasmid
    #134350
    Purpose
    Targeting vector for site specific integration of doxycycline inducible expression of full length human SHH cDNA into human AAVS1 locus
    Depositor
    Lorenz Studer
    Publication
    Cederquist et al Nat Biotechnol. 2019 Apr;37(4):436-444.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SHH (SHH Human)
    Expression
    Mammalian
    Available since
    July 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    134350-plasmid-map-sequence-id-305675

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  18. pCEP4-PDGFRA-reporter

    Plasmid
    #136456
    Purpose
    Mammalian fluorescent reporter plasmid for PDGFRA signaling.
    Depositor
    Erik Procko
    Publication
    Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    PDGFRalpha (PDGFRA Human)
    Array of serum response elements (SRE) to drive destabilized GFP expression from a minimal promoter.
    Tags
    Extracellular c-myc epitope tag, Signal peptide f…
    Expression
    Mammalian
    Promoter
    CMV and Minimal promoter with artificial SRE enha…
    Available since
    July 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    136456-plasmid-map-sequence-id-263271

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Showing: 8901 - 8920 of 30881 results