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27,462 results

Showing: 11701 - 11720 of 27462 results

  1. Click editor (CE1) mRNA expression vector - T7-5'UTR-PCV2-nCas9-EcKlenow-3'UTR-polyA (CJT472)

    Plasmid
    #217808
    Purpose
    Expression vector for mRNA production of the click editor construct (CE1; PCV2-nCas9-EcKlenow)
    Depositor
    Benjamin Kleinstiver
    Publication
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    5'UTR-PCV2-XTEN-nSpCas9-BPNLS-EcKlenow(exo-)-BPNLS-3'UTR
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)
    Promoter
    T7
    Available since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217808-plasmid-map-sequence-id-489173

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  4. pPB-TetOn-FUS-CHOP-FusionRed-FRB-iLID-WPRE-SFFV-MBP-SspB-irFP-NLS-WPRE

    Plasmid
    #223287
    Purpose
    A complete OptoMBP cassette for dissolution of FUS-CHOP condensates.
    Depositor
    Jared Toettcher
    Publication
    Brumbaugh-Reed et al Nat Commun. 2024 Aug 7;15(1):6717. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    myc-FUS-CHOP-FusionRed-FRB-iLID, MBP-SspB-irFP-NLS
    Use
    Synthetic Biology; Piggybac transposase insertabl…
    Expression
    Mammalian
    Available since
    Aug. 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    223287-plasmid-map-sequence-id-442312

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  7. Actin Binding Domain Tension Sensor

    Plasmid
    #215368
    Purpose
    Expresses Actin Binding Domain Tension Sensor (ABDTS), composed of Ftractin-Linker-TSMod-Linker-Ftractin. TSMod is the tension sensor module containing mTFP1 and mVenus.
    Depositor
    Brenton Hoffman
    Publication
    Shoyer et al Cell Rep Methods. 2024 Jul 15;4(7):10081
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Actin Binding Domain Tension Sensor
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    215368-plasmid-map-sequence-id-430713

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  8. Actin Binding Domain Tensionless Control

    Plasmid
    #215371
    Purpose
    Expresses Actin Binding Domain Tensionless Control (ABDTL), composed of Ftractin-Linker-TSMod. This is the unloaded control for Actin Binding Domain Tension Sensor (ABDTS).
    Depositor
    Brenton Hoffman
    Publication
    Shoyer et al Cell Rep Methods. 2024 Jul 15;4(7):10081
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Actin Binding Domain Tensionless Control
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    215371-plasmid-map-sequence-id-430717

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  10. TDP-REGv2(mScarlet-FLAG)#11 in pTwist-CMV backbone

    Plasmid
    #216155
    Purpose
    Expresses mScarlet in cells with TDP-43 loss-of-function. Very bright and decent dynamic range (>10-fold), slightly leaky. It uses a single intron. [Code 'B12']. Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments
    Depositor
    Pietro Fratta
    Publication
    Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mScarlet with cryptic splice site and C-terminal FLAG tag
    Expression
    Mammalian
    Available since
    July 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    216155-plasmid-map-sequence-id-437763

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  12. gCBEv3

    Plasmid
    #220618
    Purpose
    vector for encoding an engineered glycosylase-based cytosine (C) base editor (gCBEv3) driven by EF1a promoter, guide RNAs compatible with SpG driven by hU6, mCherry driven by CBh promoter
    Depositor
    Huawei Tong
    Publication
    Tong et al Nat Commun. 2024 Jun 8;15(1):4897. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    nSpCas9(Nterm)-XTEN-hUNG2-XTEN-Cas9(Cterm)
    Expression
    Mammalian
    Mutation
    hUNG2(del1-88;N213D,K184A,A214V), SpCas9(D10A)
    Promoter
    hU6, EF1a
    Available since
    June 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    220618-plasmid-map-sequence-id-436973

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  15. pCAG2-EGFP-C-IFT144

    Plasmid
    #218740
    Purpose
    Expresses C-terminally EGFP-tagged IFT144 in mammalian cells
    Depositor
    Kazuhisa Nakayama
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    IFT144 (WDR19 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    May 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    218740-plasmid-map-sequence-id-434235

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  16. CXCR7-DuET

    Plasmid
    #213224
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Publication
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CXCR7 (ACKR3 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213224-plasmid-map-sequence-id-430786

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  18. Intron-Tagging-EGFP-Frame0-Parental-Minicircle

    Plasmid
    #216124
    Purpose
    Parental minicircle containing EGFP flanked by a splice acceptor and splice donor. Used with other intron tagging plasmids for placing the EGFP tag as a synthetic exon into frame 0 introns of target genes.
    Depositor
    Stefan Kubicek
    Publication
    Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA-SA-(GGGGS)x4-EGFP-(GGGGS)x4-SD
    Use
    CRISPR
    Available since
    April 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    216124-plasmid-map-sequence-id-428729

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  19. pGL3-SEC61B.N-BSD.P2A.miniIAA7.3xFlag

    Plasmid
    #216250
    Purpose
    HDR template to tag endogenous human SEC61B N-terminus with BSD.P2A.miniIAA7.3xFlag
    Depositor
    Elina Ikonen
    Publication
    Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HDR template for human SEC61B (SEC61B Synthetic, HDR template, Human)
    Use
    CRISPR; Hdr template
    Tags
    BSD.P2A.miniIAA7.3xFlag
    Expression
    Mammalian
    Available since
    March 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    216250-plasmid-map-sequence-id-426858

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Showing: 11701 - 11720 of 27462 results