30,420 results

1. pCdrA-gfp(ASV)C

   Plasmid

   #111615

   Purpose

   Fluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species

   Depositor

   Tim Tolker-Nielsen

   Article

   Rybtke et al Appl Environ Microbiol. 2012 Aug;78(15):

   Insert

   PcdrA-RBSII-gfp(ASV)-T0-T1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   June 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFLP3

   Plasmid

   #64946

   Purpose

   Flp recombinase expression vector

   Depositor

   Herbert Schweizer

   Article

   Choi et al Nat Methods. 2005 Jun;2(6):443-8.

   Insert

   Flp recombinase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lambda promoter/temperature-sensitive Lambda cI r…

   Available Since

   Nov. 2, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pCA24N-ligase

   Plasmid

   #87741

   Purpose

   IPTG-inducible expression of T4 DNA ligase for protein purification

   Depositor

   Wayne Patrick

   Article

   Wilson et al Protein Eng Des Sel. 2013 Jul;26(7):471-

   Insert

   T4 DNA ligase (30 )
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T5-lac

   Available Since

   March 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRSETB/mStayGold

   Plasmid

   #212017

   Purpose

   mStayGold has an n1 adaptor at its N-terminus and is useful for C-terminal tagging. This construct is used also for expression of mStayGold itself.

   Depositor

   Atsushi Miyawaki

   Article

   Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

   Insert

   mStayGold
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Jan. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCdrA-gfpC

   Plasmid

   #111614

   Purpose

   Fluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species

   Depositor

   Tim Tolker-Nielsen

   Article

   Rybtke et al Appl Environ Microbiol. 2012 Aug;78(15):

   Insert

   PcdrA-RBSII-gfp(Mut3)-T0-T1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   June 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMyNT-kan

   Plasmid

   #84693

   Purpose

   Protein expression in mycobacteria

   Depositor

   Matthias Wilmanns

   Article

   Beckham et al Protein Sci. 2020 Oct 2. doi: 10.1002/pr

   Type

   Empty backbone

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   Acetamidase

   Available Since

   Jan. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. eGFP-luciferase-C1

   Plasmid

   #163523

   Purpose

   Expresses both eGFP and firefly luciferase in mammalian cells

   Depositor

   Thomas Weber

   Insert

   Firefly luciferase
   

   Use

   Luciferase

   Tags

   eGFP

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRBC-sfGFP-150TAG

   Plasmid

   #174076

   Purpose

   Plasmid with p15a origin of replication expressing codon optimized superfolder GFP-150 TAG with C-terminal His6 tag, under T7 promoter,

   Depositor

   Ryan Mehl

   Article

   Zhu et al ACS Chem Biol. 2019 Jul 19;14(7):1564-15

   Insert

   Superfolder GFP
   

   Use

   Tags

   His-6

   Expression

   Bacterial

   Mutation

   N150TAG

   Promoter

   T7

   Available Since

   Nov. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET-29b(+)_NSP4ss_SerM_NucA-His

   Plasmid

   #218190

   Purpose

   Bacterial expression of a periplasmically expressed His-tagged Serratia Marcescens NucA (Benzonase)

   Depositor

   Florence Pojer

   Insert

   nucA
   

   Use

   Tags

   His and NSP4 signal secretion peptide

   Expression

   Bacterial

   Mutation

   codon optimized ecoli residues - 22-266

   Promoter

   T7

   Available Since

   May 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSIJ8

    Plasmid

    #68122

    Purpose

    Temperature sensitive plasmid for either lambda Red recombinase genes or flippase recombinase expression

    Depositor

    Alex Nielsen

    Article

    Jensen et al Sci Rep. 2015 Dec 8;5:17874. doi: 10.103

    Inserts

    flp recombinase
    rhaS
    rhaR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    rhamnose

    Available Since

    Dec. 8, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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