30,260 results

1. pSF-CjPglB

   Plasmid

   #199305

   Purpose

   pSN18 derivative encoding C.jejuni PglB with C-terminal FLAG epitope tag

   Depositor

   Michael Jewett

   Article

   Hershewe et al Nat Commun. 2021 Apr 22;12(1):2363. doi:

   Insert

   CjPglB with flag tag
   

   Use

   Tags

   Flag tag

   Expression

   Bacterial

   Mutation

   Promoter

   pBAD

   Available since

   May 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. WasCFP /pQE-30

   Plasmid

   #79858

   Purpose

   Cyan-green fluorescent protein with tryptophan-based chromophore in equilibrium between anionic and neutral states.

   Depositor

   Konstantin Lukyanov

   Article

   Sarkisyan et al Sci Rep. 2012;2:608. doi: 10.1038/srep00

   Insert

   WasCFP
   

   Use

   Tags

   HisTag

   Expression

   Bacterial

   Mutation

   Cyan fluorescent protein mCerulean with substitut…

   Promoter

   T5 promoter / lac operator

   Available since

   Sept. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMRE132

   Plasmid

   #118486

   Purpose

   broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

   Depositor

   Mitja Remus-Emsermann

   Article

   Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

   Insert

   sGFP2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pNC01

   Plasmid

   #233643

   Purpose

   For bacterial expression of sfGFP c-terminally tagged with VitelloTagA.

   Depositor

   Zak Swartz

   Article

   Clarke et al Development. 2024 Oct 15;151(20):dev2028

   Insert

   sfGFP with c-terminal VitelloTagA
   

   Use

   Tags

   6-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a-mTurquoise2-CNA35

   Plasmid

   #61602

   Purpose

   bacterial expression of N-terminal fusion protein of CNA35 with m-Turquoise-2

   Depositor

   Maarten Merkx

   Article

   Aper et al PLoS One. 2014 Dec 9;9(12):e114983. doi:

   Insert

   mTurqouise and CNA35
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. g5s pD42

   Plasmid

   #117452

   Purpose

   Expression of Cyth2

   Depositor

   Richard Kahn

   Insert

   Cyth2 (CYTH2 Human)
   

   Use

   Tags

   V5-His6

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pQE30-His-ThrRS

   Plasmid

   #124119

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Sebastian Maerkl, Takuya Ueda

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Threonyl-tRNA ligase (thrS Escherichia coli)
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSABAD14-172

   Plasmid

   #61976

   Purpose

   PhoRadAC6 intein

   Depositor

   Hideo Iwai

   Article

   Aranko et al Mol Biosyst. 2014 May;10(5):1023-34. doi

   Insert

   PhoRadAC6 intein
   

   Use

   Tags

   GB1-H6

   Expression

   Bacterial

   Mutation

   Promoter

   pBAD

   Available since

   Feb. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBBR1.cas9-NT

   Plasmid

   #197871

   Purpose

   Constitutive expression of Cas9 endonuclease, trancRNA and crRNA with a randomly generated spacer sequence (non-targeting)

   Depositor

   Baojun Wang

   Article

   Huan et al ACS Synth Biol. 2023 Mar 17;12(3):709-72

   Inserts

   trancCRNA
   Cas9
   crRNA (non-chromosomal (E. coli/S. flexneri)-targeting spacer sequence)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. MBP-ch-TOG-His6

    Plasmid

    #69108

    Purpose

    For bacterial expression of recombinant human ch-TOG tagged with MBP and His.

    Depositor

    Stephen Royle

    Article

    Nixon et al Elife. 2015 Jun 19;4. doi: 10.7554/eLife

    Insert

    Colonic Hepatic Tumour Over-expressed Gene (CKAP5 Human)
    

    Use

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSARSF53-165

    Plasmid

    #61817

    Purpose

    PhoRadAdC6 intein

    Depositor

    Hideo Iwai

    Article

    Aranko et al Mol Biosyst. 2014 May;10(5):1023-34. doi

    Insert

    PhoRadAdC6 intein
    

    Use

    Tags

    H6-Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28a-SpyTagMBP

    Plasmid

    #35050

    Depositor

    Mark Howarth

    Article

    Zakeri et al Proc Natl Acad Sci U S A. 2012 Mar 20;10

    Insert

    SpyTagMBP
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Simple peptide tag added to the N-terminus of MBP…

    Promoter

    T7 promoter

    Available since

    April 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1.Gα12-LgB115

    Plasmid

    #134363

    Purpose

    Nanoluc complementation assay. Expression of Gα12 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 115 and 116 of Gα12.Addition of the HA epitope at N terminus of Gα12.

    Depositor

    Julien Hanson

    Article

    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40

    Insert

    Gα12-LgB115 (GNA12 Human)
    

    Use

    Tags

    HA

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    T7

    Available since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. Poliovirus 1 PV1 isolate 09_05_19 3C protease

    Plasmid

    #228647

    Purpose

    Bacterial expression of the Polio 3C protease

    Depositor

    ASAP Discovery, Frank von Delft

    Insert

    Polio virus 3C protease
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 18, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pKJ0464

    Plasmid

    #228716

    Purpose

    Bacterial expression of T7 RNAP with E643G mutation; for protein production and purification

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Jiang et al Science 0, eadr6006

    Insert

    SUmo_T7pol_E643G
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    E643G

    Promoter

    Available since

    Nov. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHelper_AraC/pBad-Tnpase (camR)

    Plasmid

    #213909

    Purpose

    Arabinose-inducible himar1C9 transposase expression - cam resistance

    Depositor

    James Collins

    Article

    English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.

    Insert

    AraC/pBad ATc-inducible himar1C9 transposase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJH628_pJH_LldR_PlldR-sfGFP.3TAG

    Plasmid

    #163727

    Purpose

    Lactate-inducible expression of green fluorescent protein encoding three amber codons

    Depositor

    Matthew Bennett

    Article

    Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

    Insert

    lldr gfp.3TAG
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    gfp.N39TAG,N135TAG,Y151TAG

    Promoter

    Available since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMF440

    Plasmid

    #62550

    Purpose

    Broad host range plasmid for constitutive expression of mCherry. Designed for expression in Pseudomonas aeruginosa.

    Depositor

    Michael Franklin

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    trc

    Available since

    April 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET21a-His-IleRS

    Plasmid

    #124112

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Sebastian Maerkl, Takuya Ueda

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Isoleucyl-tRNA ligase (ileS Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pQE60-EFTu-His

    Plasmid

    #124127

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Sebastian Maerkl, Takuya Ueda

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Elongation factor Tu (tufB Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge