33,902 results

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pBHM2403

Plasmid

#171687

Purpose

pRS316-PTEF1-CnoCas9, contains Cryptococcus neoformans codon optimized Cas9 under the control of the TEF1 promoter and terminator.

Depositor

Hiten Madhani

Publication

Huang et al Genetics. 2021 Oct 23. pii: 6409193. doi

Insert

CnoCAS9

Use

CRISPR

Expression

Bacterial and Yeast

Promoter

TEF1

Available since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pG106 - KA

Plasmid

#178041

Purpose

Bacteroides - Escherichia coli shuttle vector with ErmF and ErmAM, Kan and Amp selection markers

Depositor

Janina Lewis

Publication

Jones et al Mol Oral Microbiol. 2020 Aug;35(4):181-1

Type

Empty backbone

Use

Shuttle vector

Expression

Bacterial

Available since

Feb. 1, 2022

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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pCDF-1b-RFC140ΔN555

Plasmid

#175043

Purpose

Overexpression of human (Rfc1) p140ΔN555 in E.coli

Depositor

Brian Kelch

Publication

Gaubitz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

Insert

Replication factor C subunit 1 (RFC1 Human)

Expression

Bacterial

Promoter

T7 promoter

Available since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pDF0118 DisCas7-11 bacterial expression with Twinstrep-SUMO-Codon

Plasmid

#172503

Purpose

Encodes the DisCas7-11 protein only for bacterial recombinant protein expression for purification

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Publication

Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

Insert

DisCas7-11 bacterial expression with Twinstrep-SUMO-Codon

Expression

Bacterial

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pPSU15

Plasmid

#173828

Purpose

Expresses 15 kD Penn State ladder protein

Depositor

Song Tan

Publication

Santilli et al Sci Rep. 2021 Aug 18;11(1):16703. doi: 1

Insert

HSTPABPAC

Expression

Bacterial

Promoter

T7

Available since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pSL1145 (pSPIN, pBBR1 backbone, R*MmeI)

Plasmid

#160736

Purpose

Single-plasmid V. cholerae CAST, encodes all proteins, crRNA, and donor DNA. Non-targeting crRNA with BsaI sites for spacer cloning. Mini-tn has MmeI site in R end for Tn-seq. pBBR1 backbone.

Depositor

Samuel H. Sternberg

Publication

Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

Insert

VchCAST proteins, crRNA, and donor DNA

Expression

Bacterial

Promoter

J23119

Available since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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pAGM22082_sfGFP1-10

Plasmid

#153515

Purpose

E.coli expression plasmid for the production of GFP1-10

Depositor

Sylvestre Marillonnet

Publication

Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

Insert

sfGFP1-10

Expression

Bacterial

Available since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCDFDuet-nsp7-nsp8

Plasmid

#159092

Purpose

Coexpression construct of Nsp7 with an N-terminal His-tag and Nsp8

Depositor

Seth Darst

Publication

Chen et al Cell. 2020 Sep 17;182(6):1560-1573.e13.

Inserts

NSP7
NSP8

Tags

His

Expression

Bacterial

Promoter

T7

Available since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pJ4M_hnRNPA2_FL_WT

Plasmid

#139109

Purpose

Express FL hnRNPA2 with a C-terminal MBP tag

Depositor

Nicolas Fawzi

Publication

Ryan et al Nucleic Acids Res. 2020 Sep 1. pii: 5900

Insert

hnRNPA2 (HNRNPA2B1 Human)

Expression

Bacterial

Mutation

none

Available since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCutamp

Plasmid

#140632

Purpose

Plasmid-curing in Escherichia coli by targeting the AmpR promoter

Depositor

Sheng Yang

Publication

Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

Insert

SpCas9_lambda-RED system, SacB, Rha induction system, sgRNA targeting AmpR promoter

Use

CRISPR

Expression

Bacterial

Available since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pSC101-Donor

Plasmid

#140630

Purpose

Kanamycin resistance donor for ShCAST_harboring the temperature sensitive pSC101 origin of replication.

Depositor

Sheng Yang

Publication

Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

Insert

LE, RE, KanR, temperature sensitive pSC101 origin of replication

Use

CRISPR; Transposon

Expression

Bacterial

Available since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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pXT130_TwinStrep-SUMO-ShTnsC

Plasmid

#135526

Purpose

Protein expression construct for ShTnsC

Depositor

Feng Zhang

Publication

Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

Insert

ShTnsC

Tags

TwinStrep-SUMO

Expression

Bacterial

Available since

Dec. 18, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pTOX5

Plasmid

#127450

Purpose

Encodes MqsR toxin and amilCP chromoprotein. CAM-R

Depositor

Matthew Waldor

Publication

Lazarus et al Appl Environ Microbiol. 2019 Jun 14. pii

Inserts

mqsR toxin
amilCP chromoprotein

Expression

Bacterial

Mutation

Codon optimized from protein sequence in FPbase

Promoter

pRha and tac

Available since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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RP1B FUS 1-163

Plasmid

#127192

Purpose

Protein expression for affinity purification

Depositor

Nicolas Fawzi

Publication

Murthy et al Nat Struct Mol Biol. 2019 Jul;26(7):637-

Insert

FUS 1-163 (FUS Human)

Tags

hexahistidine

Expression

Bacterial

Available since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pCpf1b

Plasmid

#122187

Purpose

Bacterial genome editing plasmid with kanamycin resistance marker and using sacB as a counter-selection marker

Depositor

Cheng-Cai Zhang

Publication

Niu et al ACS Synth Biol. 2018 Dec 10. doi: 10.102

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Available since

March 12, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pET-28b-T7-henAsCas12a(E174R/S542R/K548R)-NLS(nucleoplasmin)-6xHis (AAS1885)

Plasmid

#114072

Purpose

T7 promoter bacterial expression plasmid for human codon optimized enAsCas12a(E174R/S542R/K548R) with C-terminal NLS and His-tag

Depositor

Keith Joung, Benjamin Kleinstiver

Publication

Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

Insert

human codon optimized enAsCas12a (E174R/S542R/K548R) with C-terminal NLS and His-tag

Tags

NLS(nucleoplasmin) and 6xHis

Expression

Bacterial

Mutation

E174R, S542R, and K548R

Available since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pBECKP-spe

Plasmid

#117236

Purpose

ncas9-BEC, fusion protein of rAPOBEC1 and spCas9 nickase

Depositor

Quanjiang Ji

Publication

Wang et al Appl Environ Microbiol. 2018 Sep 14. pii

Insert

ncas9-BEC, fusion protein of rAPOBEC1 and spCas9 nickase

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDSG419

Plasmid

#115600

Purpose

surface-displayed Ag2; medium copy

Depositor

Ingmar Riedel-Kruse

Publication

Glass et al Cell. 2018 Jul 26;174(3):649-658.e16. do

Insert

regulated adhesin construct

Expression

Bacterial

Available since

Oct. 5, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAJM.661

Plasmid

#108532

Purpose

TtgRAM + PTtg-YFP Reporter. Nar sensor for use in non-Marionette strain.

Depositor

Christopher Voigt

Publication

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

TtgRAM + PTtg-YFP

Expression

Bacterial

Mutation

TtgR: R130H A144T A148V

Available since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pCdrA-gfp(ASV)C

Plasmid

#111615

Purpose

Fluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species

Depositor

Tim Tolker-Nielsen

Publication

Rybtke et al Appl Environ Microbiol. 2012 Aug;78(15):

Insert

PcdrA-RBSII-gfp(ASV)-T0-T1

Expression

Bacterial

Available since

June 4, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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