29,693 results

1. pDest-527

   Plasmid

   #11518

   Purpose

   Gateway destination vector for bacterial expression; His tag

   Depositor

   Dominic Esposito

   Type

   Empty backbone

   Use

   Gateway destination vector

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7-lacO (lactose/IPTG inducible)

   Available since

   May 24, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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2. CAPN1

   Plasmid

   #25607

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   CAPN1 (CAPN1 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Contains amino acids 29-360

   Promoter

   Available since

   Oct. 25, 2011

   Availability

   Industry, Academic Institutions, and Nonprofits

3. pMRE155

   Plasmid

   #118505

   Purpose

   broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

   Depositor

   Mitja Remus-Emsermann

   Article

   Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

   Insert

   mScarlet-I
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET12a-PAPI

   Plasmid

   #19961

   Depositor

   Jonathan Tait

   Article

   Wood et al Blood. 1996 Sep 1. 88(5):1873-80.

   Insert

   Annexin V (ANXA5 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 16, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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5. UbcH5c WT pET28a

   Plasmid

   #12643

   Depositor

   Rachel Klevit

   Article

   Brzovic et al Mol Cell. 2006 Mar 17. 21(6):873-80.

   Insert

   UbcH5c - Ubiquitin conjugating enzyme (UBE2D3 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 31, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET15bpATnp

   Plasmid

   #121137

   Purpose

   This vector encode a His6-tagged proteinA-Tnp(hyperactive form) fusion protein.

   Depositor

   Keji Zhao

   Article

   Carter et al Nat Commun. 2019 Aug 20;10(1):3747. doi:

   Insert

   Protein A-Tnp
   

   Use

   Tags

   his

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. p12x601

   Plasmid

   #157785

   Purpose

   EcoRV digestion produces 12x601 chromatin assembly construct with central TetO following PEG removal of plasmid backbone

   Depositor

   Michael Rosen

   Article

   Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

   Insert

   pMD2.G(spei/spei)&12x601_tetO
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET30-R5-GFP

   Plasmid

   #17838

   Depositor

   Jay Keasling

   Article

   Shaikh et al Anal Chem. 2008 Feb 1. 80(3):886-90.

   Insert

   pET30-GFP
   

   Use

   Synthetic Biology

   Tags

   His, R5, and S-tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 14, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMRE-Tn7-151

   Plasmid

   #118565

   Purpose

   miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

   Depositor

   Mitja Remus-Emsermann

   Article

   Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

   Insert

   mTurquoise2
   

   Use

   Minitn7 delivery vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBB541

    Plasmid

    #27394

    Depositor

    Bernd Bukau

    Article

    de Marco et al BMC Biotechnol. 2007 . 7():32.

    Insert

    groESL (groL, groS E. coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHH0103_EEF1A1_GTP-EFTU

    Plasmid

    #109861

    Purpose

    Protein expression and purification of EEF1A1_GTP-EFTU

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    EEF1A1_GTP-EFTU (EEF1A1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. NSUN2

    Plasmid

    #188059

    Purpose

    Bacterial expression plasmid for human NSUN2; for activity testing

    Depositor

    Tanja Schirmeister

    Article

    Schwickert et al J Med Chem. 2022 Jul 18. doi: 10.1021/ac

    Insert

    NSUN2 (NSUN2 Human)
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Aug. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBBR1_DVK_AE(J23100-RiboJ-UTR1-E0040m-B0015)

    Plasmid

    #197662

    Purpose

    Expresses GFP under the control of J23100 and RiboJ-UTR1

    Depositor

    Eric Young

    Article

    Keating et al ACS Synth Biol. 2023 Jun 9. doi: 10.1021

    Insert

    GFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMUT2-pFF-41.5-mCherry

    Plasmid

    #192863

    Purpose

    pMUT2 based vector for sensing oxygen in E. coli Nissle 1917

    Depositor

    Morten Sommer

    Article

    Vaaben et al ACS Synth Biol. 2022 Dec 16;11(12):4184-

    Insert

    Oxygen inducible promoter
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSUB7

    Plasmid

    #169698

    Purpose

    Template plasmid for generating C-terminal 6-His epitope tag fusions to bacterial genes by "Lambda red" recombineering.

    Depositor

    Nara Figueroa

    Article

    Uzzau et al Proc Natl Acad Sci U S A. 2001 Dec 18;98

    Insert

    6xHis epitope tag adjacent to FRT-flanked aph (KanR) gene
    

    Use

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLG005 (Retron-TIR)

    Plasmid

    #157883

    Purpose

    Expresses the retron-TIR defense system

    Depositor

    Feng Zhang

    Article

    Gao et al Science. 2020 Aug 28;369(6507):1077-1084

    Insert

    Retron-TIR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBAD_GFP11_T7LysH17A

    Plasmid

    #59591

    Purpose

    Expression vector for solubility screening of proteins or protein fragments using a split-GFP assay.

    Depositor

    Richard Kingston

    Article

    Bulloch et al Anal Biochem. 2014 Jul 10. pii: S0003-26

    Type

    Empty backbone

    Use

    Tags

    6xHis-tag and GFP11

    Expression

    Bacterial

    Mutation

    Promoter

    pARA

    Available since

    May 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFUM

    Plasmid

    #133130

    Purpose

    A recombinant plasmid expressing fum (fumarase) gene

    Depositor

    Gregg Beckham

    Article

    Guarnieri et al Appl Environ Microbiol. 2017 Aug 17;83(1

    Insert

    fumarase gene
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PEP-carboxykinase promoter

    Available since

    Jan. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pH6HTC_PRDX6-Halo

    Plasmid

    #175338

    Purpose

    Production of HaloTag fusion protein

    Depositor

    Claudia Kutter

    Article

    Atanasoai et al bioRxiv 2021.11.08.467743

    Insert

    PRDX6 (PRDX6 Human)
    

    Use

    Cell free expression

    Tags

    HaloTag-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCoofy4

    Plasmid

    #43986

    Depositor

    Sabine Suppmann

    Article

    Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

    Type

    Empty backbone

    Use

    Tags

    His6-MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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