34,175 results

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pTOX5

Plasmid

#127450

Purpose

Encodes MqsR toxin and amilCP chromoprotein. CAM-R

Depositor

Matthew Waldor

Publication

Lazarus et al Appl Environ Microbiol. 2019 Jun 14. pii

Inserts

mqsR toxin
amilCP chromoprotein

Expression

Bacterial

Mutation

Codon optimized from protein sequence in FPbase

Promoter

pRha and tac

Available since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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RP1B FUS 1-163

Plasmid

#127192

Purpose

Protein expression for affinity purification

Depositor

Nicolas Fawzi

Publication

Murthy et al Nat Struct Mol Biol. 2019 Jul;26(7):637-

Insert

FUS 1-163 (FUS Human)

Tags

hexahistidine

Expression

Bacterial

Available since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pET-28b-T7-henAsCas12a(E174R/S542R/K548R)-NLS(nucleoplasmin)-6xHis (AAS1885)

Plasmid

#114072

Purpose

T7 promoter bacterial expression plasmid for human codon optimized enAsCas12a(E174R/S542R/K548R) with C-terminal NLS and His-tag

Depositor

Keith Joung, Benjamin Kleinstiver

Publication

Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

Insert

human codon optimized enAsCas12a (E174R/S542R/K548R) with C-terminal NLS and His-tag

Tags

NLS(nucleoplasmin) and 6xHis

Expression

Bacterial

Mutation

E174R, S542R, and K548R

Available since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pBECKP-spe

Plasmid

#117236

Purpose

ncas9-BEC, fusion protein of rAPOBEC1 and spCas9 nickase

Depositor

Quanjiang Ji

Publication

Wang et al Appl Environ Microbiol. 2018 Sep 14. pii

Insert

ncas9-BEC, fusion protein of rAPOBEC1 and spCas9 nickase

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDSG419

Plasmid

#115600

Purpose

surface-displayed Ag2; medium copy

Depositor

Ingmar Riedel-Kruse

Publication

Glass et al Cell. 2018 Jul 26;174(3):649-658.e16. do

Insert

regulated adhesin construct

Expression

Bacterial

Available since

Oct. 5, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAJM.661

Plasmid

#108532

Purpose

TtgRAM + PTtg-YFP Reporter. Nar sensor for use in non-Marionette strain.

Depositor

Christopher Voigt

Publication

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

TtgRAM + PTtg-YFP

Expression

Bacterial

Mutation

TtgR: R130H A144T A148V

Available since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pTU2-AmyE-Pveg-eGFP

Plasmid

#112792

Purpose

Expresses GFP under the control of Pveg promoter and it is integrated into AmyE locus. Used to test correct transformation

Depositor

Chris French

Publication

Wicke et al bioRxiv 185108

Insert

Pveg-eGFP

Expression

Bacterial

Available since

Aug. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pSANG14-3F

Plasmid

#39265

Depositor

John McCafferty

Publication

Martin et al BMC Biotechnol. 2006 Dec 7;6:46.

Type

Empty backbone

Tags

6xHis, Alkaline phosphatase, pelB leader, and tri…

Expression

Bacterial

Promoter

T7

Available since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pBAD-GINKO1

Plasmid

#113111

Purpose

Bacterial expression of genetically encoded green fluorescent potassium ion indicator GINKO1

Depositor

Robert Campbell

Publication

Shen et al Commun Biol. 2019 Jan 14;2:18. doi: 10.1

Insert

GINKO1

Tags

6-His Tag, T7 tag (gene 10 leader), and Xpress™ t…

Expression

Bacterial

Promoter

araBAD

Available since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pCdrA-gfp(ASV)C

Plasmid

#111615

Purpose

Fluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species

Depositor

Tim Tolker-Nielsen

Publication

Rybtke et al Appl Environ Microbiol. 2012 Aug;78(15):

Insert

PcdrA-RBSII-gfp(ASV)-T0-T1

Expression

Bacterial

Available since

June 4, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pJM253 (miniCTX1-rhaSR-PrhaBAD)

Plasmid

#110571

Purpose

Integration vector for Pseudomonas aeruginosa with rhaSR-PrhaBAD inducible promoter

Depositor

Joanna Goldberg

Publication

Meisner et al Appl Environ Microbiol. 2016 Oct 27;82(2

Insert

rhaSR-PrhaBAD

Expression

Bacterial

Promoter

rhaSR-PrhaBAD

Available since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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pYES1L-URA

Plasmid

#84301

Purpose

E.coli shuttle vector for large-scale DNA assembly in S. cerevisiae.

Depositor

George Church

Publication

Ostrov et al Science. 2016 Aug 19;353(6301):819-22. d

Type

Empty backbone

Expression

Bacterial and Yeast

Available since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pET28a-MH6-RspWYL1

Plasmid

#108306

Purpose

Expresses E. coli codon optimized RspWYL1 in the pET28a backbone.

Depositor

Arbor Biotechnologies

Publication

Yan et al Mol Cell. 2018 Mar 9. pii: S1097-2765(18

Insert

RspWYL1

Use

CRISPR

Tags

mH6 tag

Expression

Bacterial

Promoter

lac

Available since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pT7-7 asyn A30P

Plasmid

#105726

Purpose

Bacterial expression of mutant human alpha synuclein

Depositor

Hilal Lashuel

Publication

Rospigliosi et al J Mol Biol. 2009 May 22;388(5):1022-32.

Insert

alpha-synuclein A30P (SNCA Human)

Expression

Bacterial

Mutation

A30P

Promoter

T7

Available since

April 2, 2018

Availability

Academic Institutions and Nonprofits only

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pET14b – lambda exonuclease

Plasmid

#104531

Purpose

bacterial expression of lambda exonuclease

Depositor

Charles Bell

Publication

Zhang et al Proc Natl Acad Sci U S A. 2011 Jul 19;10

Insert

λ exonuclease

Tags

His6 followed by a thrombin site

Expression

Bacterial

Promoter

T7

Available since

Dec. 18, 2017

Availability

Academic Institutions and Nonprofits only

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RNAPII_CTD27-52

Plasmid

#98678

Purpose

Expresses 6xHis-tagged C-terminal domain of RNA polymerase II

Depositor

Nicolas Fawzi

Publication

Janke et al Biochemistry. 2017 Oct 11. doi: 10.1021/

Insert

RNA polymerase II C-terminal domain (POLR2A Human)

Tags

6xHis

Expression

Bacterial

Available since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pAC-SP6

Plasmid

#102418

Purpose

Ac transposase with SP6 promoter for in vitro transcription

Depositor

Tatjana Sauka-Spengler

Publication

Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

Insert

Ac transposase

Expression

Bacterial

Promoter

SP6

Available since

Oct. 30, 2017

Availability

Academic Institutions and Nonprofits only

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AcrIIA4

Plasmid

#101043

Purpose

Anti-CRISPRprotein which inhibit SpyCas9 and LmoCas9 activity.

Depositor

Zhiwei Huang

Publication

Dong et al Nature. 2017 Jun 15;546(7658):436-439. d

Insert

AcrIIA4

Tags

GST

Expression

Bacterial

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pXY027

Plasmid

#98915

Purpose

Express FtsZ tagged with GFP in E coli

Depositor

Jie Xiao

Publication

Buss et al PLoS Genet. 2015 Apr 7;11(4):e1005128. d

Insert

FtsZ

Tags

GFP

Expression

Bacterial

Promoter

T5-lac

Available since

Aug. 10, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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GST-thr-RAF1-RBD

Plasmid

#86033

Purpose

Expresses the RAS-binding domain of RAF1 for in vitro analysis of RAS activation

Depositor

Dominic Esposito

Insert

RAF1 RBD (Ras-binding domain) (RAF1 Human)

Tags

GST

Expression

Bacterial

Mutation

Contains residues 1-149

Promoter

T7

Available since

Jan. 12, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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