29,693 results

1. His-MBP human SPOP (aa 28-337)

   Plasmid

   #52294

   Purpose

   bacterial expression of human SPOP (aa 28-337) fused to His-MBP

   Depositor

   Brenda Schulman

   Article

   Zhuang et al Mol Cell. 2009 Oct 9;36(1):39-50. doi: 1

   Insert

   SPOP (SPOP Human)
   

   Use

   Tags

   His-MBP and TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 11, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pAF-HdrB3

   Plasmid

   #184908

   Purpose

   carrying CRISPR/Cas9 system for genome editing in Acidithiobacillus ferridurans

   Depositor

   Radhakrishnan Mahadevan

   Article

   Chen et al Environ Sci Technol. 2023 Aug 22;57(33):

   Insert

   Cas9-sgRNA-homology arms
   

   Use

   CRISPR; Broad-host-range

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSU315

   Plasmid

   #169697

   Purpose

   Template plasmid for generating C-terminal hemagglutinin (1xHA) epitope tag fusions to bacterial genes by "Lambda red" recombineering.

   Depositor

   Nara Figueroa

   Article

   Uzzau et al Proc Natl Acad Sci U S A. 2001 Dec 18;98

   Insert

   1xHA epitope tag adjacent to FRT-flanked aph (KanR) gene
   

   Use

   Tags

   1xHA tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEVOL-ABK

   Plasmid

   #126035

   Purpose

   Efficient expression of tRNA synthetase/tRNA for the incorporation of a photo-cross-linker, 3’-azibutyl-N-carbamoyl-lysine (AbK) into recombinant protein by amber codon (TAG) suppression in E. coli.

   Depositor

   Andrea Musacchio

   Article

   Pan et al Nat Commun. 2019 Sep 6;10(1):4046. doi:

   Inserts

   tRNA synthetase 1
   tRNA synthetase 2
   tRNA for TAG codon
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   L274M, C313A, Y349F

   Promoter

   araBAD promoter, glnS promoter, and proK promoter

   Available since

   Sept. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAL-rfp_T

   Plasmid

   #74075

   Purpose

   pAL374 plasmid carrying the rfp (T) sgRNA targeting the template strand of rfp, SpecR

   Depositor

   Timothy Lu

   Article

   Cleto et al ACS Synth Biol. 2016 Feb 16.

   Insert

   sgRNA rfp (T)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   ptac

   Available since

   April 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKSJ340

   Plasmid

   #27125

   Depositor

   Alan Finkelstein

   Article

   Jakes et al Mol Microbiol. 2010 Feb;75(3):567-78. do

   Insert

   Colicin Ia + Ia immunity protein
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 20, 2011

   Availability

   Academic Institutions and Nonprofits only

7. pQE81XN-mStayGold(J)

   Plasmid

   #225445

   Purpose

   Expresses Hisx6-Tagged mStayGold(J) in bacteria

   Depositor

   Benjamin Glick

   Insert

   mStayGold(J)
   

   Use

   Tags

   6X-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEX4T1-human beta-catenin full length

   Plasmid

   #132609

   Purpose

   expresses human beta-catenin full length in E. coli

   Depositor

   Wenqing Xu

   Article

   Xing et al Structure. 2008 Mar;16(3):478-87. doi: 1

   Insert

   beta-catenin
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGST2-Rabex5 (9-73)

   Plasmid

   #17630

   Depositor

   James Hurley

   Article

   Lee et al Nat Struct Mol Biol. 2006 Mar . 13(3):26

   Insert

   Rabex5 (9-73) (RABGEF1 )
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Ubiquitin-binding domain: residues 9-73

   Promoter

   Available since

   March 28, 2008

   Availability

   Academic Institutions and Nonprofits only

10. pCS6

    Plasmid

    #55752

    Purpose

    Produces T7 RNA Polymerase.

    Depositor

    Matthew Bennett

    Article

    Schmidt et al ACS Synth Biol. 2012 Oct 19;1(10):445-50

    Insert

    T7 RNA Polymerase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    July 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. mNeptune-pBAD

    Plasmid

    #54714

    Purpose

    Localization: Protein Expression Vector , Excitation: 600, Emission: 650

    Depositor

    Robert Campbell, Roger Tsien, Nathan Shaner, Michael Davidson

    Article

    Lin et al Chem Biol. 2009 Nov 25;16(11):1169-79. d

    Type

    Empty backbone

    Use

    Tags

    6xHis and mNeptune

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 25, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pET28-iLOVf

    Plasmid

    #63723

    Purpose

    E coli expression plasmid to produce Thrombin cleavable His tagged iLOV fusion proteins

    Depositor

    Brian Smith

    Type

    Empty backbone

    Use

    Tags

    TEE, iLOV, 6xHis, thrombin cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAL-pck_T

    Plasmid

    #74068

    Purpose

    pAL374 plasmid carrying the pck (T) sgRNA targeting, the template strand of pck, SpecR

    Depositor

    Timothy Lu

    Article

    Cleto et al ACS Synth Biol. 2016 Feb 16.

    Insert

    sgRNA pck (T)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    ptac

    Available since

    April 11, 2016

    Availability

    Academic Institutions and Nonprofits only

14. 3xFLAG-CUL5-pCMV7.1

    Plasmid

    #155024

    Purpose

    N-terminal 3xFLAG-tagged CUL5 in a pCMV7.1 vector

    Depositor

    Andre Catic

    Article

    Sweeney et al Sci Rep. 2020 Aug 18;10(1):13942. doi: 1

    Insert

    Cullin 5 (CUL5 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Bacterial

    Mutation

    Promoter

    pCMV7.1

    Available since

    Sept. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHH0103_ESRRB_Hormone-recep

    Plasmid

    #109909

    Purpose

    Protein expression and purification of ESRRB_Hormone-recep

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    ESRRB_Hormone-recep (ESRRB Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAT219

    Plasmid

    #134887

    Purpose

    Bacterial expression

    Depositor

    Thomas Howard

    Article

    Azubuike et al N Biotechnol. 2021 Nov 25;65:20-30. doi:

    Insert

    mRFP1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET15TV-L-hlPYK WT

    Plasmid

    #187975

    Purpose

    Expresses the human liver pyruvate kinase

    Depositor

    Audrey Lamb

    Article

    McFarlane et al Acta Crystallogr F Struct Biol Commun. 2

    Insert

    hlPYK WT
    

    Use

    Tags

    Hexahistag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRSFDuet-1_VNp-mNeongreen_OmpA-mCherry

    Plasmid

    #182390

    Purpose

    Bacterial expression of Vesicle Nucleating peptide-mNeongreen and ompA-mCherry fusions

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Inserts

    VNp-mNeongreen-His
    OmpA-mCherry
    

    Use

    Tags

    Vesicle Nucleating peptide (VNp)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pF

    Plasmid

    #42519

    Depositor

    Terry Magnuson

    Article

    Jang et al PLoS One. 2013;8(2):e57075. doi: 10.1371

    Type

    Empty backbone

    Use

    Unspecified; Cloning

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7, T3

    Available since

    March 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEC2935 (p7INT-P23_mNeongreen~ssrA (LDD variant))

    Plasmid

    #218508

    Purpose

    integrative plasmid enabling constitutive expression of a destabilized mNeongreen reporter in Streptococcus pyogenes, targets the attP site of phage T12 in several S. pyogenes strains via an integrase

    Depositor

    Emmanuelle Charpentier

    Article

    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

    Insert

    mNeongreen
    

    Use

    Tags

    modified ssrA degradation tag

    Expression

    Bacterial

    Mutation

    deletion of MCS containing Plac_lacZa, introducti…

    Promoter

    P23

    Available since

    July 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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