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Plasmid#22806DepositorInsert
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 28, 2010AvailabilityAcademic Institutions and Nonprofits only -
pJS183
Plasmid#134142PurposeBacterial expression of GFP-Ska1 and Ska2DepositorInsertHis-GFP-human Ska1; human Ska2
UseTagsGFPExpressionBacterialMutationPromoterAvailable sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCT5-bac1.8
Plasmid#119871PurposeConstitutive expression of sfGFP gene in Bacillus subtilis, B. megaterium, and Escherichia coliDepositorInsertsCymR'
sfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationSNP in 592 bpPromoterPserA promoter and Pveg promoter with CuO sequenceAvailable sinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTriEx4-Cdc42-2G
Plasmid#68814PurposeFRET-based biosensor reporting on endogenous Cdc42 activation (for expression in E. coli, insect and vertebrate cells)DepositorInsertCdc42 activation reporter
UseTagsHis and MycExpressionBacterial, Insect, and Mamm…MutationPromoterCMVAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMP2463
Plasmid#107774PurposeExpression of EGFPDepositorInsertpLacZ-EGFP in pBBR1-mcs5
UseTagspLacZ-EGFPExpressionBacterialMutationPromoterpLacZAvailable sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTHSSe_47
Plasmid#109245PurposepSC101 var5 BbsI cloning vectorDepositorInsertsfGFP
UseTagsExpressionBacterialMutationPromoterPJ23105Available sinceJan. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCJ189
Plasmid#162666PurposepET-21b(+) based plasmid for expresion of MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by an 8 residue Gly/Ser linker; codon optimized for expression in E. coli K12, with C-terminal His tag.DepositorInsertMHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by an 8 residue Gly/Ser linker
UseTagsHisExpressionBacterialMutationcodon optimized for expression in E. coli K12PromoterT7/lacAvailable sinceFeb. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
6972 pPROEX-HTb-SSA1
Plasmid#1231DepositorInsertSsa1 (SSA1 Budding Yeast)
UseTagshis tag (tev cleavable)ExpressionBacterialMutationPromoterAvailable sinceAug. 9, 2005AvailabilityAcademic Institutions and Nonprofits only -
pRK172-α-syn-TEV-GFP
Plasmid#166671PurposeBacterial expression of alpha synuclein coupled to GFP via a TEV site for uptake studiesDepositorInsertmouse synuclein (from V. Lee) (Snca Mouse)
UseTagsGFPExpressionBacterialMutationadded a TEV cleavage site sequence between synucl…PromoterCMVAvailable sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMB1-A-TelR
Plasmid#204979PurposeLevel 1 MoClo plasmid for A. baumannii with additional Tellurite resistance cassette for multi-drug resistance strainsDepositorInsertmRFP1
UseSynthetic BiologyTagsExpressionBacterialMutationAdded replications elements for Acinetobacter spe…PromoterAvailable sinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
Tn7 integration plasmid
Plasmid#154134PurposeSuicidal integration plasmid with the Tn7 arms, streptomycin and kanamycin resistance genes and the restriction site to clone the barcode-carrying cassette.DepositorInsertsleft and right end of Tn7 arm
Spectinomycin resistant gene
UseTagsExpressionBacterialMutationPromoterSpectinomycin resistance gene promoter and noAvailable sinceJuly 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pL99
Plasmid#78577PurposeOver-expression shuttle vector for gene cloning and transfer from E. coli to Streptomyces. Contains inducible PnitA-NitR systemDepositorTypeEmpty backboneUseStreptomyces expressionTagsExpressionBacterialMutationPromoterinducible PnitA-NitR systemAvailable sinceMay 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28a-mCherryGGBsMTS
Plasmid#133625PurposeExpression vector for mCherry fused to a membrane targeting sequence (MTS) of the B.subtilis MinD (C-terminal fusion) and an N-terminal His-tag for purification.DepositorInsertmCherryGGBsMTS
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pETduet+sfGFP-TetR
Plasmid#157794PurposeExpression of sfGFP-TetR fusion proteinDepositorInsertsfGFP-TetR
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
3xFLAG-CUL2-pCMV7.1
Plasmid#155020PurposeN-terminal 3xFLAG-tagged CUL2 in a pCMV7.1 vectorDepositorInsertCullin 2 (CUL2 Human)
UseTags3xFLAGExpressionBacterialMutationPromoterpCMV7.1Available sinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSMART-HCKan-EV
Plasmid#202466PurposeEmpty vector of pSMART-HCKanDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
HHM-iATPSnFR1.0
Plasmid#102546Purposeexpresses ATP sensor in bacteriaDepositorInsertiATPSnFR1.0
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pSLC-242
Plasmid#73189PurposeTemplate plasmid which encodes chloramphenicol resistance gene for positive selection and a toxin gene (relE) under the control of rhamnose induceable promoter (PrhaB) for negative selection.DepositorInsertchloramphenicol (cat Although Ampicillin gene is present, it was inactivated while cloning.)
UseTagsExpressionBacterialMutationPromoterchloramphenicol promoterAvailable sinceJuly 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEB2-E2-Crimson
Plasmid#104010PurposePlasmid encoding E2-CrimsonDepositorInsertE2-Crimson
UseLow copyTagsExpressionBacterialMutationMutations relative to DsRed (R2D, S4T, K5E, E10P,…PromoterproCAvailable sinceDec. 27, 2017AvailabilityAcademic Institutions and Nonprofits only