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pET-6xHis-(pos9)GFP

Plasmid

#89247

Purpose

Expresses GFP with +9 theoretical charge in E.Coli

Depositor

David Liu

Article

Thompson et al Chem Biol. 2012 Jul 27;19(7):831-43. doi

Insert

His6-(pos9)-GFP

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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pTriEx-RhoA-Q63L_mScarlet-i_SGFP2

Plasmid

#85072

Purpose

Green/red FRET biosensor for RhoA in the GTP locked constitutively active state

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

RhoA Biosensor constitutively active (Q63L) (RHOA Human)

Tags

6xHis and mScarlet-i and SGFP2

Expression

Bacterial, Insect, and Mamm…

Mutation

Q63L

Promoter

CMV

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pCR2.1-InC

Plasmid

#83959

Purpose

Contains a synthetic insert to serve as internal control DNA template for a Salmonella Real-Time PCR assay.

Depositor

Kai-Shun Chen

Insert

Internal Control sequence

Expression

Bacterial

Available Since

Nov. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pTKEI-Tre-C04

Plasmid

#79754

Purpose

Single fluorescent protein biosensor for trehalose

Depositor

David Savage

Article

Nadler et al Nat Commun. 2016 Jul 29;7:12266. doi: 10

Insert

Tre-C04

Tags

6xHis

Expression

Bacterial

Promoter

pTrc

Available Since

July 25, 2016

Availability

Academic Institutions and Nonprofits only

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pBTR(hCc)

Plasmid

#61026

Purpose

Expresses horse holocytochrome c in E. coli

Depositor

Gary Pielak

Article

Patel et al Protein Expr Purif. 2001 Jul;22(2):220-4

Insert

horse cytochrome c (CYCS Equus*)

Tags

None

Expression

Bacterial

Mutation

*see comments

Available Since

Nov. 17, 2015

Availability

Academic Institutions and Nonprofits only
S96 ELP

Plasmid

#68393

Purpose

Elastin-like polypeptide (VPGxG) with 96 repeats of Serine guest residue

Depositor

Andrew MacKay

Article

Shah et al Protein Sci. 2012 Jun;21(6):743-50. doi:

Insert

S96 ELP

Expression

Bacterial

Promoter

T7

Available Since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only

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MBP-MEK1

Plasmid

#68300

Purpose

N-terminal MBP tagged human MEK1 gene for bacterial expression

Depositor

Jesse Rinehart

Article

Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

Insert

MBP-MEK1 (MAP2K1 Synthetic, Human)

Tags

His and MBP

Expression

Bacterial

Available Since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pRF-TetR

Plasmid

#49374

Depositor

Christopher Voigt

Article

Stanton et al Nat Chem Biol. 2013 Dec 8. doi: 10.1038/

Insert

TetR repressor

Use

Synthetic Biology

Expression

Bacterial

Available Since

Aug. 25, 2015

Availability

Academic Institutions and Nonprofits only

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pUC18T-mini-Tn7T-Tp-dsRedExpress

Plasmid

#65036

Purpose

TpR mini-Tn7 vector, mobilizable, for RFP tagging bacteria

Depositor

Herbert Schweizer

Article

Choi et al Nat Protoc. 2006;1(1):153-61.

Insert

dsRedExpress

Expression

Bacterial

Available Since

Aug. 14, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pGEX6p1-GST-Rnf31

Plasmid

#63134

Purpose

Expression of human RNF31/HOIP codon optimized for E. coli and insect cells

Depositor

Titia Sixma

Article

Smit et al EMBO J. 2012 Oct 3;31(19):3833-44. doi:

Insert

Rnf31 (RNF31 Synthetic, Human)

Tags

GST

Expression

Bacterial

Mutation

Synthetic based on human protein sequence

Promoter

tac

Available Since

March 16, 2015

Availability

Academic Institutions and Nonprofits only
pTE102

Plasmid

#59397

Purpose

MethyloBrick expression vector, mxaF promoter; TcR

Depositor

Tobias Erb

Article

Schada von Borzyskowski et al ACS Synth Biol. 2014 Aug 8.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Promoter

PmxaF

Available Since

Dec. 30, 2014

Availability

Academic Institutions and Nonprofits only

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pCM271rfp

Plasmid

#59774

Purpose

pCMrfp with TrfA mutation (271R to C)

Depositor

Nathan Hillson

Article

Bi et al Microb Cell Fact. 2013 Nov 13;12:107. do

Insert

RFP

Expression

Bacterial

Promoter

pBAD

Available Since

Nov. 21, 2014

Availability

Academic Institutions and Nonprofits only

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pBAD.Sigma32.I54N

Plasmid

#59982

Purpose

Expresses sigma32 I54N mutant in E. coli to render a heat-shock like cellular folding environment

Depositor

Jeffery Kelly

Article

Zhang et al ACS Chem Biol. 2014 Jul 22.

Insert

Sigma32 I54N

Expression

Bacterial

Mutation

I54N

Available Since

Nov. 13, 2014

Availability

Academic Institutions and Nonprofits only

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pET11a-link-NGFP

Plasmid

#52732

Purpose

IPTG-inducible expression of link-NGFP negative control for in vivo split GFP assembly assay

Depositor

Lynne Regan

Article

Magliery et al J Am Chem Soc. 2005 Jan 12;127(1):146-57

Insert

link-NGFP

Tags

His tag

Expression

Bacterial

Promoter

T7

Available Since

June 20, 2014

Availability

Academic Institutions and Nonprofits only

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pTD103aiiA(Cm)

Plasmid

#48886

Purpose

Negative feedback plasmid from oscillator

Depositor

Jeff Hasty

Article

Prindle et al Nature. 2011 Dec 18;481(7379):39-44. doi

Insert

aiiA and LuxR

Use

Synthetic Biology

Tags

ssrA tag (AANDENYALAA)

Expression

Bacterial

Promoter

pLux

Available Since

Feb. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pET His6 MBP prescission LIC cloning vector (HMPKS)

Plasmid

#29721

Depositor

Scott Gradia

Type

Empty backbone

Tags

6xHis, MBP, and Prescission cleavage site

Expression

Bacterial

Promoter

T7-lacO (lactose/IPTG inducible)

Available Since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pOSIP-KL (KanR, Lambda)

Plasmid

#45984

Depositor

Drew Endy, Keith Shearwin

Article

St-Pierre et al ACS Synth Biol. 2013 Sep 20;2(9):537-41.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 26, 2013

Availability

Academic Institutions and Nonprofits only

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pOPINTRX

Plasmid

#41116

Depositor

Ray Owens

Article

Bird et al Methods. 2011 Sep;55(1):29-37. Epub 2011

Type

Empty backbone

Expression

Bacterial, Insect, and Mamm…

Available Since

March 27, 2013

Availability

Academic Institutions and Nonprofits only

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pQTEV-PSMD11

Plasmid

#31333

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

proteasome (prosome, macropain) 26S subunit, non-ATPase, 11 (PSMD11 Human)

Tags

His and TEV

Expression

Bacterial

Available Since

March 22, 2013

Availability

Academic Institutions and Nonprofits only

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pAK405

Plasmid

#37114

Depositor

Julia Vorholt

Article

Kaczmarczyk et al Appl Environ Microbiol. 2012 May;78(10):

Type

Empty backbone

Expression

Bacterial

Available Since

Aug. 8, 2012

Availability

Academic Institutions and Nonprofits only

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