30,738 results

1. BtARR3 long

   Plasmid

   #236266

   Purpose

   Bacterial expression of bovine arrestin-3 long isoform

   Depositor

   Vsevolod Gurevich

   Insert

   arrestin-3 (ARR3 Bovine)
   

   Expression

   Bacterial

   Mutation

   long isoform

   Promoter

   lac

   Available Since

   May 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. BtARR2 Cys-less

   Plasmid

   #236273

   Purpose

   Bacterial expression of bovine arrestin-2 C59V, C125S, C140L, C150V, C242V, C251V, C269S

   Depositor

   Vsevolod Gurevich

   Article

   Zhuo et al J Biol Chem. 2014 Jul 25;289(30):20991-1

   Insert

   arrestin-2 (ARRB2 Bovine)
   

   Expression

   Bacterial

   Mutation

   C59V, C125S, C140L, C150V, C242V, C251V, C269S

   Promoter

   lac

   Available Since

   May 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. BtARR2 long

   Plasmid

   #236268

   Purpose

   Bacterial expression of bovine arrestin-2 long isoform

   Depositor

   Vsevolod Gurevich

   Insert

   arrestin-2 (ARRB2 Bovine)
   

   Expression

   Bacterial

   Mutation

   long isoform

   Promoter

   lac

   Available Since

   May 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET24TEV-tSHIP1

   Plasmid

   #183770

   Purpose

   Expresses C-terminal truncated SHIP1 (tSHIP1)with C-terminal TEV and 6HIS tags from pET24 backbone

   Depositor

   William Kerr

   Article

   Sudan et al Front Immunol. 2022 May 4;13:830961. doi

   Insert

   SHIP1 (INPP5D Human)
   

   Tags

   6HIS and Tobacco Etch Virus (TEV) Cleavage site

   Expression

   Bacterial

   Mutation

   includes aa1 to aa883 only

   Promoter

   T7 lac

   Available Since

   June 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEANHISECArdB

   Plasmid

   #96967

   Purpose

   Expression of E. coli ArdB protein wirh an N-terminal TEV protease cleavable His tag

   Depositor

   Huanting Liu, James Naismith

   Article

   Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

   Insert

   E. coli ArdB
   

   Tags

   N-ter TEV cleavable 6His

   Expression

   Bacterial

   Mutation

   none

   Promoter

   T7

   Available Since

   Aug. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pVRa14_3200

   Plasmid

   #49666

   Depositor

   Christopher Voigt

   Article

   Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

   Insert

   ECF14_3200
   

   Use

   Synthetic Biology

   Tags

   His6-PreScission

   Expression

   Bacterial

   Mutation

   Codon optimized for E.coli

   Promoter

   pT7-lacO

   Available Since

   Jan. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pACλCI-β-flap (831-1057)

   Plasmid

   #53733

   Purpose

   Expression of λCI fused via three alanines to E. coli RNAP beta subunit, aa 831-1057 (β-flap). Can be cut with Not1 and BamHI to replace β-flap with a moiety of interest.

   Depositor

   Ann Hochschild

   Article

   Dove et al Nature. 1997 Apr 10;386(6625):627-30.

   Insert

   λCI-β-flap fusion
   

   Expression

   Bacterial

   Promoter

   lac UV5

   Available Since

   June 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBRα-β flap (831-1057)

   Plasmid

   #53734

   Purpose

   Expression of alpha NTD fused via three alanines to E. coli RNAP subunit beta, aa 831-1057 (β-flap). Can be cut with Not1 and BamHI to replace β-flap with a moiety of interest.

   Depositor

   Ann Hochschild

   Article

   Dove et al Nature. 1997 Apr 10;386(6625):627-30.

   Insert

   alpha NTD-Beta flap fusion
   

   Expression

   Bacterial

   Promoter

   lpp/lacUV5 (tandem promoter)

   Available Since

   June 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAW014-2

   Plasmid

   #85612

   Purpose

   Multi-gRNA delivery vector containing ugtP-gRNA.P395T for Bacillus subtilis

   Depositor

   Perry Chou

   Article

   Westbrook et al Appl Environ Microbiol. 2016 Jun 3. pii:

   Insert

   ugtP-gRNA.P395T
   

   Expression

   Bacterial

   Available Since

   Jan. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJH-MBP-CbAgo

    Plasmid

    #127707

    Purpose

    pET His6 MBP TEV CbAgo

    Depositor

    John van der Oost

    Article

    Hegge et al Nucleic Acids Res. 2019 May 9. pii: 5487

    Insert

    CbAgo
    

    Tags

    MPB

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMJ922

    Plasmid

    #78312

    Purpose

    Expression of His6-MBP-tagged Cas9-NLS-EGFP protein in E. coli

    Depositor

    Martin Jinek

    Article

    Burger et al Development. 2016 Apr 29. pii: dev.13480

    Insert

    SpCas9
    

    Tags

    HA-2xNLS-EGFP-NLS and His6-MBP-TEV

    Expression

    Bacterial

    Mutation

    human codon-optimized gene sequence

    Promoter

    T7

    Available Since

    June 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-HA-Tuba

    Plasmid

    #22214

    Depositor

    Pietro De Camilli

    Article

    Salazar et al J Biol Chem. 2003 Dec 5. 278(49):49031-4

    Insert

    Tuba (DNMBP Human)
    

    Tags

    HA

    Expression

    Bacterial and Mammalian

    Available Since

    Oct. 9, 2009

    Availability

    Academic Institutions and Nonprofits only

13. pFCiR2

    Plasmid

    #167512

    Purpose

    Broad-Host range expression vector, RSF1010 origin, CinRAM activator, PCin promoter, mRFP, Kanamycin Resistance Marker.

    Depositor

    Christopher Reisch

    Article

    Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

    Insert

    CinRAM-mRFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    PCin

    Available Since

    May 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHH0103_DYNLL1_Dynein-light

    Plasmid

    #110006

    Purpose

    Protein expression and purification of DYNLL1_Dynein-light

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    DYNLL1_Dynein-light (DYNLL1 Human)
    

    Expression

    Bacterial

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBAD-Citron1

    Plasmid

    #134300

    Purpose

    Bacterial expression of direct-response citrate sensor

    Depositor

    Robert Campbell

    Article

    Zhao et al ACS Cent Sci. 2020 Aug 26;6(8):1441-1450

    Insert

    Citron1
    

    Tags

    6-His tag

    Expression

    Bacterial

    Promoter

    pBAD

    Available Since

    April 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. CeNL_pRSETb

    Plasmid

    #89510

    Purpose

    Cyan color variant of bright luminescent protein for bacterial expression

    Depositor

    Takeharu Nagai

    Article

    Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

    Insert

    CeNL
    

    Tags

    His X 6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pACEC3GH (Hyg)

    Plasmid

    #110084

    Purpose

    Replicative, acetamide-based expression vector containing a C-terminal 3C cleavage site and a GPF-fusion with a His10-tag. Suitable for high-level expression levels amenable for large scale purification of proteins.

    Depositor

    Markus Seeger

    Article

    Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

    Type

    Empty backbone

    Tags

    3C cleavage site - GFP - His10 Tag

    Expression

    Bacterial

    Available Since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCB578

    Plasmid

    #141095

    Purpose

    E. coli-Lactobacilli shuttle vector containing SpCas9, tracrRNA, and a repeat-spacer-repeat array

    Depositor

    Chase Beisel

    Article

    Leenay et al Biotechnol J. 2019 Mar;14(3):e1700583. d

    Inserts

    Cas9 and tracrRNA
    repeat-spacer-repeat array
    

    Expression

    Bacterial

    Available Since

    April 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMRE-Tn7-151

    Plasmid

    #118565

    Purpose

    miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    mTurquoise2
    

    Use

    Minitn7 delivery vector

    Expression

    Bacterial

    Available Since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSPAsp-hp

    Plasmid

    #48122

    Purpose

    Shuttle vector E. coli/B.meg.; encoding the predicted optimal signal peptide for protein secretion using Bacillus megaterium under control of the xylose inducible promoter

    Depositor

    Dieter Jahn

    Article

    Stammen et al Appl Environ Microbiol. 2010 Jun;76(12):

    Insert

    predicted optimal signal peptide for Bacillus megaterium protein secretion
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Oct. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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