29,693 results

1. NylCA-TS_pET28a

   Plasmid

   #215419

   Purpose

   Expression construct for NylCA-TS gene, codon optimized for expression in E. coli

   Depositor

   Gregg Beckham

   Article

   Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

   Insert

   NylCA-TS
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   S111G/A137L/E263Q

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. NylCp2_pET28a

   Plasmid

   #215420

   Purpose

   Expression construct for NylCp2 gene, codon optimized for expression in E. coli

   Depositor

   Gregg Beckham

   Article

   Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

   Insert

   NylCp2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. NylCp2-TS_pET28a

   Plasmid

   #215421

   Purpose

   Expression construct for NylCp2-TS gene, codon optimized for expression in E. coli

   Depositor

   Gregg Beckham

   Article

   Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

   Insert

   NylCp2-TS
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   D122G/H130Y/D36A/E263Q

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBz15_Strep_Rubisco

   Plasmid

   #140839

   Purpose

   wild-type Halothiobacillus neapolitanus Form I Rubisco with StrepII tag (CbbL-StrepII, CbbS), pET-14b backbone

   Depositor

   David Savage

   Article

   Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

   Insert

   Form IA Rubisco; CbbL, CbbS
   

   Use

   Tags

   Strep II

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pWZ699

   Plasmid

   #208117

   Purpose

   Payload yeast assembly vector with TK-mActb on backbone

   Depositor

   Jef Boeke

   Article

   Zhang et al Nature. 2023 Nov 1. doi: 10.1038/s41586-

   Type

   Empty backbone

   Use

   Synthetic Biology; Copy number inducible

   Tags

   Expression

   Bacterial, Mammalian, and Y…

   Mutation

   Promoter

   Human PGK1 for thymidine kinase

   Available since

   Nov. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pWM_12x601_45bpLinker

   Plasmid

   #157791

   Purpose

   EcoRV digestion produces 12x601 chromatin assembly construct with 45 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes

   Depositor

   Michael Rosen

   Article

   Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

   Insert

   12x601_45bp linker
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBolux

   Plasmid

   #188109

   Purpose

   Encodes a promoter-less Bacteroides-optimized lux cassette

   Depositor

   Guy Townsend

   Article

   Modesto et al Nat Commun. 2023 Jan 17;14(1):275. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. HyperNova/pRSETB

   Plasmid

   #221456

   Purpose

   Fast-maturating photosensitizing fluorescent protein for chromophore-assisted light inactivation

   Depositor

   Kiwamu Takemoto

   Article

   Shidara et al Commun Biol. 2024 Aug 6;7(1):945. doi: 1

   Insert

   HyperNova
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMC059-HisMS2_PLP_NucPhos_pac

   Plasmid

   #155039

   Purpose

   Generation of MS2 Virus Like Particles packaged with the sequence for the SARS-CoV-2 Nucleocapsid Phosphoprotein gene

   Depositor

   Paul Freemont

   Article

   Crone et al Nat Commun. 2020 Sep 8;11(1):4464. doi:

   Inserts

   Maturation Protein
   Coat Protein Dimer
   Nucleocapsid Phosphoprotein
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Remove TypeIIs Restriction Sites

   Promoter

   T7

   Available since

   Sept. 9, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pHAGE2-TetOminiCMV-OSKM

    Plasmid

    #136541

    Purpose

    Dox-inducible polycistronic reprogramming lentiviral vector expressing mouse Oct4, Sox2, Klf4, cMyc

    Depositor

    Hans Schöler

    Article

    Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

    Insert

    Oct4-P2A-Sox2-T2A-Klf4-E2A-cMyc (Sox2, Pou5f1, Myc, Klf4 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tetO-miniCMV (dox-inducible)

    Available since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLIC-His-RGS4

    Plasmid

    #204847

    Purpose

    Express His tagged-RGS4 in bacterial cells for protein purification

    Depositor

    Mikel Garcia-Marcos

    Article

    Park et al Mol Cell. 2023 Jul 20;83(14):2540-2558.e

    Insert

    RGS4 (RGS4 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGEX-2T-Rac1-Q61L

    Plasmid

    #12979

    Depositor

    Gary Bokoch

    Insert

    Rac1 constitutively active (RAC1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Q61L Constitutively active

    Promoter

    Available since

    Oct. 27, 2006

    Availability

    Academic Institutions and Nonprofits only

13. pENTR11 CA NFAT1

    Plasmid

    #11792

    Depositor

    Anjana Rao

    Insert

    NFAT1 (Nfatc2 Mouse)
    

    Use

    Tags

    HA

    Expression

    Bacterial

    Mutation

    Constitutively active: multiple serine to alanine…

    Promoter

    Available since

    Aug. 7, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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14. Ub-GyrA

    Plasmid

    #128737

    Purpose

    Expresses Ub fused to GyrA intein at the C-terminal

    Depositor

    Sharon Rozovsky

    Article

    Liu et al Chembiochem. 2019 Jul 2. doi: 10.1002/cb

    Insert

    UBC
    

    Use

    Tags

    Mxe GyrA

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 18, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pMBP-FnCas12a

    Plasmid

    #113432

    Purpose

    Expresses 10xHis-MBP fused to FnCas12a

    Depositor

    Jennifer Doudna

    Article

    Chen et al Science. 2018 Apr 27;360(6387):436-439.

    Insert

    FnCas12a
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. BirA His-tag pLEMO

    Plasmid

    #119817

    Purpose

    Express endogenous biotin ligase in E.coli

    Depositor

    Alexander Gabibov

    Article

    Lomakin et al Bioconjug Chem. 2019 Apr 25. doi: 10.102

    Insert

    Biotin ligase
    

    Use

    Tags

    6-Histidine-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET28_hTLN2-FL

    Plasmid

    #164835

    Purpose

    Expression of FL human talin2

    Depositor

    Tina Izard

    Article

    Rangarajan et al J Biol Chem. 2020 Sep 11;295(37):12885-1

    Insert

    TLN2 (TLN2 Human)
    

    Use

    Tags

    His8

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pXD70CT5.1-PxylR-CymR, Pveg(2CuO)-LacZ

    Plasmid

    #191628

    Purpose

    E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1 (tandem CymR binding sites)-LacZ

    Depositor

    Emily Balskus

    Article

    Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

    Insert

    lacZ
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pColdII-SIRPT1-Eptesicus fuscus

    Plasmid

    #78113

    Purpose

    Nt His-tag Eptesicus fuscus Sacsin 89-1368

    Depositor

    Kalle Gehring

    Insert

    Sacsin Eptesicus fuscus 89-1368
    

    Use

    Tags

    His-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGSTag

    Plasmid

    #21877

    Depositor

    David Ron

    Article

    Ron et al Biotechniques. 1992 Dec . 13(6):866-9.

    Type

    Empty backbone

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge