Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

Applied Filters

Clear All Filters
Bacterial Expression Clear filter

Applied Filters

Clear All Filters
Bacterial Expression Clear filter

33,902 results

Showing: 2101 - 2120 of 33902 results

  3. pGEX-2T-Cdc42-T17N

    Plasmid
    #12970
    Depositor
    Gary Bokoch
    Insert
    Cdc42 DN (CDC42 Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    T17N Dominant negative
    Available since
    Oct. 27, 2006
    Availability
    Academic Institutions and Nonprofits only

  4. pGEX-2T-Rac1-T17N

    Plasmid
    #12978
    Depositor
    Gary Bokoch
    Insert
    Rac1 DN (RAC1 Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    T17N Dominant negative
    Available since
    Oct. 27, 2006
    Availability
    Academic Institutions and Nonprofits only

  6. pEVOL_PylRS(AF)-tRNA

    Plasmid
    #223511
    Purpose
    PylRS (AF)/tRNA Pyl orthogonal pair for genetic code expansion that allows site-specific incorporation of noncanonical amino-acids into a POI using the Amber stop codon
    Depositor
    Edward Lemke
    Publication
    Milles et al J Am Chem Soc. 2012 Mar 21;134(11):5187-
    Inserts
    Pyrrolysyl-tRNA Synthetase (AF) (2 copies) (pylS Methanosarcina mazei)
    tRNA Pyl
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Y306A; Y384F
    Promoter
    araBAD, rrnB, and glnS and proK
    Available since
    Oct. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    223511-plasmid-map-sequence-id-447439

    Enlarge

  8. OaAEP1_C247A

    Plasmid
    #200307
    Purpose
    Bacterial expression plasmids for production of C247A mutant of asparaginyl endopeptidase 1 from O. affinis (OaAEP1_C247A)
    Depositor
    Artur Krężel
    Publication
    Antonenko et al Bioconjug Chem. 2023 Mar 15;34(4):719-27
    Insert
    Asparaginyl endopeptidase from Oldenlandia affinis
    Tags
    Smt3
    Expression
    Bacterial
    Mutation
    Cysteine 247 to Alanine
    Promoter
    T7
    Available since
    June 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    200307-plasmid-map-sequence-id-394865

    Enlarge

  13. pcDNA3.1.D2-NP

    Plasmid
    #134366
    Purpose
    Nanoluc complementation assay. Expression of dopamine receptor D2 fused at C terminus with Natural peptide (NP) of NanoLuc. Addition of the signal sequence and Flag epitope at N terminus of D2.
    Depositor
    Julien Hanson
    Publication
    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40
    Insert
    D2-NP (DRD2 Human)
    Tags
    Flag, natural peptide of nanoluciferase, and sign…
    Expression
    Bacterial and Mammalian
    Promoter
    T7
    Available since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    134366-plasmid-map-sequence-id-306391

    Enlarge

  16. pAH-CTX1-rhadCas9

    Plasmid
    #129391
    Purpose
    Derived from pAH-CTX1-rha. The Burkholderia cenocepacia codon-optimized dCas9 cloned downstream of PrhaBAD. Hence, dCas9 is controlled by the rhamnose-inducible promoter system.
    Depositor
    Silvia Cardona
    Publication
    Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021
    Insert
    Burkholderia cenocepacia codon-optimized dCas9
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    Entire dCas9 codon optimized for the GC-rich B. c…
    Promoter
    PrhaBAD
    Available since
    March 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    129391-plasmid-map-sequence-id-273754

    Enlarge

Showing: 2101 - 2120 of 33902 results