32,387 results
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Plasmid#112574PurposeUsed for expression of protein in E. coli as TEV cleavable N-terminual 8His and Sumo fusions with optional C-terminal StrepII-tagDepositorTypeEmpty backboneTags8His and Strep tagExpressionBacterialAvailable SinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only
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pGIPZ-PD-L1-EGFP
Plasmid#120933PurposeExpress PD-L1-EGFP fusion protein in mammalian cellsDepositorInsertPD-L1-EGFP
ExpressionBacterialAvailable SinceFeb. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-N-Myc
Plasmid#119757PurposeAllows cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained N-terminal Myc tag.DepositorTypeEmpty backboneTagsGST and MycExpressionBacterialPromotertac promoterAvailable SinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUC19/DdmEGFP
Plasmid#154304Purposecloned with the codon-optimized mEGFP gene for expression in Dictyostelium discoideumDepositorInsertmEGFP
ExpressionBacterialAvailable SinceAug. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-TS2126 RNA Ligase
Plasmid#119941PurposeExpress TS2126 RNA ligase in E. coliDepositorInsertTS2126 RNA ligase
TagsHis6ExpressionBacterialPromoterT7Available SinceMay 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJSC111 - Bacterial expression plasmid for SpCas9-HF1 variant
Plasmid#101209PurposeBacterial expression plasmid for SpCas9-HF1 variantDepositorInsertSpCas9 variant N497A/R661A/Q695A/Q926A
Tags10x His, MBP, and TEV siteExpressionBacterialMutationN497A, R661A, Q695A and Q926APromoterT7Available SinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pIB163
Plasmid#90186PurposeE. coli - Streptococci shuttle plasmid for gene expression in streptococci with Pami promoterDepositorTypeEmpty backboneTagsHis-tag and Strep-tagExpressionBacterialAvailable SinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAS9counter
Plasmid#107192PurposeExpresses CAS9 and sgRNA for targeted counterselection in S. aureusDepositorInsertsCas9
sgRNA
ExpressionBacterialPromoterprab17Available SinceFeb. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNCS mCherry
Plasmid#91769PurposeBacterial expression of a fluorescent protein, monomeric Cherry (mCherry)DepositorInsertmonomeric Cherry
TagsHexa-Histidine tag, Xpress epitope for detection …ExpressionBacterialMutationSee Depositor CommentsPromoterT7Available SinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1 3xFlag-CREB
Plasmid#82721PurposeExpresses human CREBDepositorAvailable SinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMRE142
Plasmid#118494Purposebroad-host range plasmid to constitutively express fluorescent protein genes in bacteriaDepositorInsertsGFP2
ExpressionBacterialAvailable SinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pH3U3-mcs
Plasmid#12609DepositorInsertMultiple cloning site in His3 Ura3 reporter plasmid
ExpressionBacterialAvailable SinceSept. 26, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET29b-rhMOG
Plasmid#225484PurposeExpress the soluble domain of Myelin Oligodendrocyte Glycoprotein (rhMOG) in SHuffle E. coliDepositorInsertSoluble Domain of Myelin Oligodendrocyte Glycoprotein (MOG Human)
Tags6xHis tagExpressionBacterialMutationdeleted amino acids 1-29 (signal peptide) and 150…PromoterT7Available SinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSCM001
Plasmid#124605PurposeExpresses mCherry-pHluorin fusion protein in E. coli, a fluorescent reporter for cytoplasmic pHDepositorInsertmCherry-pHluorin
TagsmCherry-(Gly-Gly-Ser)x2-pHluorin translational fu…ExpressionBacterialPromoterpDABAvailable SinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCF525-EF1a-Hygro-P2A-AcrIIA4-lenti
Plasmid#115795PurposeExpresses AcrIIA4 in human cells, contained in a lentiviral vector.DepositorInsertAcrIIA4
UseLentiviralExpressionBacterial and MammalianPromoterEF-1a promoterAvailable SinceOct. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
DT034A
Plasmid#159395PurposeConstitutive LuxR expression, controlled under E.coli hybrid (J23117-J23106) promoter, and inducible Perfringolysin O (PFO) expression, controlled under V. fischeri HSL-regulated pLux (luxPR) promoterDepositorInsertpr.J23117/J23106-LuxR-pr.LuxR-PFO-pdt
UseSynthetic BiologyTagsFLAG/FLAG and pdt#3 from Collins Nat. Biotech. 20…ExpressionBacterialPromoterJ23117 and pLux promoterAvailable SinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pT7-7 asyn G51D
Plasmid#105747PurposeBacterial expression of mutant human alpha synucleinDepositorAvailable SinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGGC_RT_XVE
Plasmid#136970PurposeGoldengate/Greengate module for generation of beta-estradiole inducible genes.DepositorInsertXVE
ExpressionBacterialMutationBsaI site domestication 834 T > A,Available SinceJan. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAP05
Plasmid#186261PurposesfGFP under light light responsive PEL222 promoter and EL222 under constitutively active BBa_J2305 promoterDepositorInsertsExpressionBacterialPromoterBBa_23105 (GGCTAGCTCAGTCCTAGGTACTATGCTAGC) and PE…Available SinceSept. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-mClover3-anti-mouse-IgG(kappa)-nanobody
Plasmid#138128PurposeExpresses GST-tagged mClover3-anti-mouse-IgG(kappa)-nanobody in E. coli cells.DepositorInsertmClover3-anti-mouse-IgG(kappa)-nanobody
TagsGSTExpressionBacterialAvailable SinceJuly 30, 2020AvailabilityAcademic Institutions and Nonprofits only