30,577 results

1. OmpX

   Plasmid

   #213136

   Purpose

   Expresses outer membrane protein X

   Depositor

   Sebastian Hiller

   Article

   Rath et al Biochim Biophys Acta Biomembr. 2020 Jan

   Insert

   OmpX (24-171) (ompX E.coli (K12))
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Feb. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET21a-MTF-His

   Plasmid

   #124133

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Sebastian Maerkl, Takuya Ueda

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Methionyl-tRNA formyltransferase (fmt Escherichia coli)
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCold-TF-hPLEKHM1

   Plasmid

   #64146

   Purpose

   For protein expression in E.coli

   Depositor

   Tamotsu Yoshimori

   Article

   Tabata et al Mol Biol Cell. 2010 Dec;21(23):4162-72.

   Insert

   PLEKHM1 (PLEKHM1 Human)
   

   Use

   Tags

   6xHis-tag and Trigger Factor (TF)

   Expression

   Bacterial

   Mutation

   Promoter

   cspA

   Available Since

   April 17, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pGEX4T1 SHP1 WT

   Plasmid

   #8578

   Depositor

   Ben Neel

   Insert

   SHP1 (PTPN6 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   June 17, 2005

   Availability

   Academic Institutions and Nonprofits only

5. pQE30-His-ThrRS

   Plasmid

   #124119

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Sebastian Maerkl, Takuya Ueda

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Threonyl-tRNA ligase (thrS Escherichia coli)
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUCP20T-ecfp

   Plasmid

   #78463

   Purpose

   Broad range bacterial fluorescence labeling

   Depositor

   Mariette Barbier

   Article

   Barbier et al PLoS One. 2016 Mar 3;11(3):e0146827. doi

   Insert

   eCFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   June 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. HK2

   Plasmid

   #25529

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   HK2 (HK2 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 23, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

8. pQE30-hPolB

   Plasmid

   #70761

   Purpose

   bacterial expression of human DNA Polymerase beta (POLB)

   Depositor

   Primo Schaer

   Article

   Schuermann et al Nucleic Acids Res. 2016 Jan 4. pii: gkv1

   Insert

   POLB (POLB Human)
   

   Use

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   R333L

   Promoter

   T5/lac operon

   Available Since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBR322-Pdp1_NTD-Cre

   Plasmid

   #198274

   Purpose

   For loading Cre into PVCs; also contains PVC regulatory genes

   Depositor

   Feng Zhang

   Article

   Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

   Insert

   Pdp1_NTD-Cre
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSCM001

    Plasmid

    #124605

    Purpose

    Expresses mCherry-pHluorin fusion protein in E. coli, a fluorescent reporter for cytoplasmic pH

    Depositor

    David Summers

    Article

    Zarkan et al Sci Rep. 2019 Mar 7;9(1):3868. doi: 10.1

    Insert

    mCherry-pHluorin
    

    Use

    Tags

    mCherry-(Gly-Gly-Ser)x2-pHluorin translational fu…

    Expression

    Bacterial

    Mutation

    Promoter

    pDAB

    Available Since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pComb3XLambda

    Plasmid

    #63892

    Purpose

    Backbone pComb3X containing PCR template for human antibody lambda light chain constant region

    Depositor

    Carlos Barbas

    Article

    Andris-Widhopf et al J Immunol Methods. 2000 Aug 28;242(1-2):

    Insert

    human antibody lambda light chain (IGLL5 Human)
    

    Use

    Phage display

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lacZ

    Available Since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET21a-His-IleRS

    Plasmid

    #124112

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Sebastian Maerkl, Takuya Ueda

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Isoleucyl-tRNA ligase (ileS Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEX6P1-DEST

    Plasmid

    #119749

    Purpose

    Allows Gateway cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag.

    Depositor

    Martin Reijns, Andrew Jackson

    Type

    Empty backbone

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac promoter

    Available Since

    Jan. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pNBU2_repA2

    Plasmid

    #211911

    Purpose

    Replicative vector for protein expression in Bacteroidales with an aTc inducible promoter, terminator, and a barcoding site.

    Depositor

    Adam Arkin

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGEX-4T-1_RAF1 (51-131)

    Plasmid

    #119218

    Purpose

    purification of GST-RAF1 RBD from E. coli

    Depositor

    Helen Morrison

    Article

    Riecken et al Hum Mutat. 2015 Feb;36(2):270-8. doi: 10

    Insert

    RAF1 aa 51-131 (RAF1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Dec. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGT-Ah1

    Plasmid

    #122568

    Purpose

    MAGIC donor plasmid (Himar transposon with beta-lactamase/sfGFP payload)

    Depositor

    Harris Wang

    Article

    Ronda et al Nat Methods. 2019 Feb;16(2):167-170. doi

    Insert

    beta-lactamase/sfGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAWP78-PVCpnf_pvc13-HA

    Plasmid

    #198296

    Purpose

    Produces PVCs retargeted with HA tag

    Depositor

    Feng Zhang

    Article

    Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

    Insert

    PVCpnf_pvc13-HA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. GST-TEV-FUS

    Plasmid

    #29629

    Depositor

    Aaron Gitler

    Article

    Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

    Insert

    FUS (FUS Human)
    

    Use

    Tags

    GST and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. pComb8

    Plasmid

    #63889

    Purpose

    For phage display of antibody libraries by fusion to modified geneVIII.

    Depositor

    Carlos Barbas

    Article

    Barbas CF et al Proc Natl Acad Sci U S A. 1991 Sep 15;88

    Type

    Empty backbone

    Use

    Phage display

    Tags

    geneVIII

    Expression

    Bacterial

    Mutation

    Promoter

    lacZ

    Available Since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSH624-ssr

    Plasmid

    #199241

    Purpose

    Expresses the gene codying for the Ssr recombinase from Pseudomonas putida DOT-T1E

    Depositor

    Susanne Häußler

    Article

    Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

    Insert

    single-stranded DNA recombineering
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lacIq-Ptrc

    Available Since

    Oct. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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