32,870 results
-
Plasmid#133783PurposeLibrary backboneDepositorTypeEmpty backboneExpressionBacterialAvailable SinceJan. 31, 2020AvailabilityAcademic Institutions and Nonprofits only
-
pGEX-4T-1-3xFLAG
Plasmid#129570PurposeEmpty backbone for FLAG tagDepositorTypeEmpty backboneExpressionBacterialAvailable SinceJan. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
SpyTag003-mClover3
Plasmid#133453PurposeExpresses SpyTag003-mClover3 (a green fluorescent protein) in bacterial cytoplasmDepositorInsertSpyTag003-mClover3
TagsGSGSGSGS linker and His6ExpressionBacterialPromoterT7Available SinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAM5411
Plasmid#132663PurposeRSF1010-based broad host-range plasmid with RK2 bom site and pUC origin expressing YFP and Sp/Sm resistanceDepositorInsertsaadA
YFP
ExpressionBacterialPromoterPaadA and PconIIAvailable SinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAM5409
Plasmid#132662PurposeRSF1010-based broad host-range plasmid with RK2 bom site expressing YFP and Sp/Sm resistanceDepositorInsertsaadA
YFP
ExpressionBacterialPromoterPaadA and PconIIAvailable SinceNov. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMC037-HisMS2_PLP_pac
Plasmid#128233PurposeExpression construct for MS2 phage like particles with Esp3I cloning sites for packaged RNA.DepositorInsertsMaturation Protein
Coat Protein Dimer
ExpressionBacterialPromoterT7Available SinceNov. 15, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSL0375 (His-MBP-TniQ_Cas8_Cas7_Cas6)
Plasmid#130640PurposeExpresses V. cholerae CAST TniQ, Cas8, Cas7, and Cas6 from a T7 promoter, with N-terminal His10-MBP-TEVsite tag on TniQ for purification.DepositorInsertVchTniQ, VchCas8, VchCas7, VchCas6
UseCRISPR; TransposonTagsHis10-MBP-TEVsite on TniQExpressionBacterialPromoterT7Available SinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
hFerritinL
Plasmid#122653PurposeExpression of hFerritinL in bacterial cellsDepositorInsertlight chain ferritin, human (FTL Human)
ExpressionBacterialAvailable SinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDSW1728
Plasmid#120812PurposeE. coli-C. difficile shuttle vector: tetracycline-inducible promoter (Ptet) driving expression of red fluorescent protein (mCherryOpt)DepositorInsertred fluorescent protein (mCherry), codon-optimized for C. difficile
ExpressionBacterialPromoterPtetAvailable SinceFeb. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T-GST-eIF4E K119A
Plasmid#112818PurposeExpresses N-terminally GST-tagged mouse Eif4e K119A in bacterial cellsDepositorInsertEif4e (Eif4e Mouse)
TagsGSTExpressionBacterialMutationK119 mutated to A, increasing affinity for cap st…PromoterT7Available SinceJan. 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJMP1237
Plasmid#119262Purposefor cloning new sgRNAsDepositorInsertdcas9
ExpressionBacterialMutationD10A and H840AAvailable SinceJan. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDSG398
Plasmid#115604Purposesurface-displayed Nb3; medium copyDepositorInsertregulated adhesin construct
ExpressionBacterialMutationSee depositor comments belowAvailable SinceNov. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn7-140
Plasmid#118556PurposeminiTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertTagBFP2
UseMinitn7 delivery vectorExpressionBacterialAvailable SinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET41b+_Ufd1-His
Plasmid#117107PurposeProduction of recombinant Ufd1-His in E.coliDepositorAvailable SinceOct. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET41b+_Npl4
Plasmid#117108PurposeProduction of recombinant Npl4 in E.coliDepositorInsertNpl4 (NPLOC4 Human)
ExpressionBacterialAvailable SinceOct. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDSG358
Plasmid#115599Purposesurface-displayed Ag1; medium copyDepositorInsertregulated adhesin construct
ExpressionBacterialMutationSee depositor comments belowAvailable SinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDSG360
Plasmid#115601Purposesurface-displayed Ag3; medium copyDepositorInsertregulated adhesin construct
ExpressionBacterialAvailable SinceOct. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDSG323
Plasmid#115594Purposesurface-displayed NullDepositorInsertregulated adhesin construct
ExpressionBacterialAvailable SinceOct. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTU2-AmyE-Pveg-eGFP
Plasmid#112792PurposeExpresses GFP under the control of Pveg promoter and it is integrated into AmyE locus. Used to test correct transformationDepositorArticleInsertPveg-eGFP
ExpressionBacterialAvailable SinceAug. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHis-parallel1-NLSH2BCas9
Plasmid#112065PurposeExpression and purification of NLSH2BCas9 for fungal gene editingDepositorInsertNLS of the Fusarium oxysporum histone H2B fused to SpCas9 that was codon optimized fro Aspergillus niger
UseCRISPRTagsHis tagExpressionBacterialMutationCas9 was codon optimized for Aspergillus nigerPromoterpromoter in vectorAvailable SinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only