30,260 results

1. pEN-sacB

   Plasmid

   #196249

   Purpose

   Plasmid containing the sacB gene

   Depositor

   Bing Yang

   Article

   Li et al Commun Biol. 2023 Jan 17;6(1):56. doi: 1

   Insert

   attR2-sacB-attL3
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   sacB promoter

   Available since

   Feb. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. p2B-58

   Plasmid

   #177928

   Purpose

   Expresses yeGFP under control of doxycycline-responsive TET4 promoter (contains four rtTA binding sites)

   Depositor

   Vadim Gladyshev

   Article

   Barre et al (unpublished)

   Insert

   EGFP
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   TET4 (contains four rtTA binding sites)

   Available since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. AzoRS-4

   Plasmid

   #182884

   Purpose

   A pEvol-based plasmid containing one inducible (by arabinose) copy of the AzoRS-4 aaRS. The 2nd constitutive aaRS copy typically found in pEvol plasmids was deleted.

   Depositor

   Miriam Amiram

   Article

   Israeli et al Adv. Funct. Mater. 2021, 31, 2011276

   Insert

   AzoRS-4
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDSG290

   Plasmid

   #115609

   Purpose

   surface-displayed Nb3, used in composability experiments; low copy

   Depositor

   Ingmar Riedel-Kruse

   Article

   Glass et al Cell. 2018 Jul 26;174(3):649-658.e16. do

   Insert

   regulated adhesin construct
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pDEST17-UbE2B

   Plasmid

   #15781

   Depositor

   Wade Harper

   Article

   Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

   Insert

   Ubiquitin conjugating enzyme UbE2B (UBE2B Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 28, 2007

   Availability

   Academic Institutions and Nonprofits only

6. pMLS7-egfp

   Plasmid

   #112917

   Purpose

   Fluorescence microscopy

   Depositor

   Miguel Valvano

   Article

   Lefebre et al Appl Environ Microbiol. 2002 Dec;68(12):

   Insert

   eGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PS7 promoter and Ps7

   Available since

   Oct. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKL2310

   Plasmid

   #187875

   Purpose

   Empty pVS1-based INTEGRATE vector with loxP cargo

   Depositor

   Kan Wang

   Article

   Aliu et al Plant Biotechnol J. 2022 Jun 11. doi: 10

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119

   Available since

   Aug. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28a-SUPREM

   Plasmid

   #205023

   Purpose

   Expressing SUPer Rna Ecogii Methyltransferase (SUPREM)

   Depositor

   Paola Laurino

   Article

   Ochiai et al Nucleic Acids Res. 2024 Oct 17:gkae887.

   Insert

   SUPer Rna Ecogii Methyltransferase
   

   Use

   Tags

   His6x tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pT7C-HSP90AB1

   Plasmid

   #177659

   Purpose

   Recombinant protein synthesis of human HSP90AB1

   Depositor

   Jeff Kuret

   Article

   Ayoub et al PLoS Genet. 2023 Mar 27;19(3):e1010681.

   Insert

   HSP90AB1 (HSP90AB1 Human)
   

   Use

   Tags

   6xHis-Thrombin

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAIP4_3KT9

    Plasmid

    #234192

    Purpose

    Heterologous protein expression of aprataxin in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    3KT9 (APTX Human)
    

    Use

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. pYTK067

    Plasmid

    #65174

    Purpose

    Encodes ConR1 as a Type 5 part to be used in the Dueber YTK system

    Depositor

    John Dueber

    Article

    Lee et al ACS Synth Biol. 2015 Apr 14.

    Insert

    ConR1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. Plasmid EUPp_09

    Plasmid

    #157745

    Purpose

    PgyrA_ada _adh2 _pMB1_ampR (for expressing two enzymes (Ada and Adh2) to allow E. coli to consume ethanol)

    Depositor

    Kang Zhou

    Article

    Liang et al Metab Eng. 2020 Nov 25. pii: S1096-7176(

    Inserts

    acetaldehyde dehydrogenase
    alcohol dehydrogenase (ADH2 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PgyrA

    Available since

    June 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pETM43 p62 FL (1-440)

    Plasmid

    #210425

    Purpose

    Bacterial expression of MBP-p62 FL (1-440) with 3C cleavage site

    Depositor

    Carsten Sachse

    Article

    Tarafder et al Methods Mol Biol. 2019;1880:3-15. doi: 1

    Insert

    p62 FL (NUP62 Human)
    

    Use

    Tags

    6xHis and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRsetB-pHRed

    Plasmid

    #31472

    Depositor

    Gary Yellen

    Article

    Tantama et al J Am Chem Soc. 2011 Jul 6. 133(26):10034

    Insert

    pHRed
    

    Use

    Tags

    7xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 21, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDSG288

    Plasmid

    #115607

    Purpose

    surface-displayed Ag3, used in composability experiments; low copy

    Depositor

    Ingmar Riedel-Kruse

    Article

    Glass et al Cell. 2018 Jul 26;174(3):649-658.e16. do

    Insert

    regulated adhesin construct
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET42b-BE3

    Plasmid

    #87437

    Purpose

    Expresses BE3-NLS with an N-terminal His Tag (His6) for bacterial expression

    Depositor

    David Liu

    Article

    Rees et al Nat Commun. 2017 Jun 6;8:15790. doi: 10.

    Insert

    BE3 (Apobec1 Bacillus phage PBS2, Human, Rat)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET30a-AlkB

    Plasmid

    #79050

    Purpose

    Express wild type AlkB protein in E. coli BL21(DE3)

    Depositor

    Tao Pan

    Article

    Zheng et al Nat Methods. 2015 Sep;12(9):835-7. doi:

    Insert

    AlkB (alkB E.coli)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    A truncated AlkB with deletion of the amino (N)-t…

    Promoter

    T7

    Available since

    Aug. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBB572

    Plasmid

    #27397

    Depositor

    Bernd Bukau

    Article

    de Marco et al BMC Biotechnol. 2007 Jun 12;7:32. doi: 1

    Insert

    ibpA, ibpB (ibpB, ibpA E.coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 28, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET28b-His-hCE

    Plasmid

    #112710

    Purpose

    Expresses N-terminally His-tagged human CE/RNGTT in bacterial cells

    Depositor

    Daniel Schoenberg

    Article

    Trotman et al Nucleic Acids Res. 2017 Oct 13;45(18):10

    Insert

    RNGTT (RNGTT Human)
    

    Use

    Tags

    6xHis and Stop codons placed after RNGTT sequence…

    Expression

    Bacterial

    Mutation

    R594H

    Promoter

    T7

    Available since

    Aug. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. Tol2-mpeg1-dendra2

    Plasmid

    #51462

    Purpose

    expresses photoconvertible dendra in macrophages

    Depositor

    Anna Huttenlocher

    Article

    Harvie et al Infect Immun. 2013 Jan;81(1):110-21. doi

    Insert

    mpeg-dendra
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    mpeg1

    Available since

    May 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge