29,712 results

1. pET30-RT1306

   Plasmid

   #131521

   Purpose

   Expresses engineered RT-1306 with His-tag

   Depositor

   Bryan Dickinson

   Article

   Zhou et al Nat Methods. 2019 Dec;16(12):1281-1288.

   Insert

   RT-1306
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBLADE(FP6**)-mCherry

   Plasmid

   #168049

   Purpose

   Expresses the synthetic gene BLADE FP6 under the constitutive J23101** promoter. mCherry expression is controlled by the PBAD promoter.

   Depositor

   Barbara Di Ventura

   Article

   Romano et al Nat Chem Biol. 2021 Apr 26. pii: 10.1038

   Inserts

   Blue light-inducible AraC dimers in Escherichia coli
   mCherry
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23101** and PBAD

   Available since

   May 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. p404TEF1

   Plasmid

   #15972

   Depositor

   Fred Cross, Nicolas Buchler

   Insert

   TEF1 promoter + polylinker + CYC1 terminator (TEF1 Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   TEF1 promoter is between SacI and XbaI restrictio…

   Promoter

   Available since

   Nov. 2, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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4. pETDuet-1_VNp-mNeongreen_CydAB-mCherry

   Plasmid

   #182391

   Purpose

   Bacterial expression of Vesicle Nucleating peptide-mNeongreen and cydAB-mCherry fusions

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

   Inserts

   VNp-mNeongreen-His
   cydAB-mCherry
   

   Use

   Tags

   Vesicle Nucleating peptide (VNp)

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28-his-3C_Tau352

   Plasmid

   #100092

   Purpose

   Expresses human full-length fetal tau protein with the N-terminal 3C-cleavable His6 tag

   Depositor

   Nikolai Sluchanko

   Article

   Tugaeva et al PLoS One. 2017 Jun 2;12(6):e0178933. doi

   Insert

   Fetal microtubule-associated protein tau (MAPT Human)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   wild type sequence

   Promoter

   T7

   Available since

   Sept. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. PB-EF1a-TetOn3G

   Plasmid

   #104543

   Purpose

   PiggyBac vector encoding Tet-On 3G constitutively expressed by EF1a promoter

   Depositor

   David Vereide

   Article

   Miller et al Stem Cell Reports. 2018 Jan 9;10(1):73-8

   Insert

   Tet-On 3G
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pZE-PalkB-GFP

   Plasmid

   #44381

   Depositor

   Hal Alper

   Article

   Reed et al J Biotechnol. 2012 Apr 15;158(3):75-9. d

   Inserts

   GFP
   PalkB
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PalkB

   Available since

   April 17, 2013

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pGST-PSP-hTG

   Plasmid

   #85470

   Purpose

   bacterial expression of human tissue transglutaminase

   Depositor

   Jeffrey Keillor, Ray Truant

   Article

   Roy et al Protein Expr Purif. 2013 Jan;87(1):41-6.

   Insert

   hTG2 (TGM2 Human)
   

   Use

   Tags

   GST and PSP

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Nov. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. bNS2B47NS3

   Plasmid

   #155320

   Purpose

   co-expression of DENV4 47 peptides from NS2B cofactor and NS3 full length

   Depositor

   Dahai Luo

   Article

   Phoo et al Antiviral Res. 2020 Oct;182:104900. doi:

   Insert

   Co expression of DENV4 NS3 full length protein with 47 peptides from NS2B cofactor
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7

   Available since

   Nov. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBbS5a-OptoCre-REDMAP

    Plasmid

    #228465

    Purpose

    Light-inducible Cre recombinase split with REDMAP photoreceptors for bacterial expression

    Depositor

    Mary Dunlop

    Article

    Jafarbeglou et al ACS Synth Biol. 2024 Nov 19. doi: 10.102

    Insert

    OptoCre-REDMAP
    

    Use

    Cre/Lox

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pLacUV5

    Available since

    March 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBbAk-w4-loxP-TT-loxP-mCherry-PCB

    Plasmid

    #228469

    Purpose

    Fluorescent reporter for Cre recombinase activity and the PCB production gene. Cre removes a transcription terminator, leading to mCherry production

    Depositor

    Mary Dunlop

    Article

    Jafarbeglou et al ACS Synth Biol. 2024 Nov 19. doi: 10.102

    Inserts

    w4-loxP-TT-loxP-mCherry
    Ho1
    PcyA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23106, J23108, and T7A1

    Available since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJeM1

    Plasmid

    #135088

    Purpose

    rhamnose-inducible expression vector for replication in E. coli and P. putida

    Depositor

    Josef Altenbuchner

    Article

    Jeske et al Appl Microbiol Biotechnol. 2010 Feb;85(6

    Insert

    rhaR rhaS rhaPBAD eGFP, mob (rhaR E.coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFC333

    Plasmid

    #87844

    Purpose

    AMA1 plasmid with Aspergillus optimized Cas9 and ble selection marker

    Depositor

    Uffe Mortensen

    Article

    Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do

    Inserts

    Cas9
    ble (bleomycin resistance marker)
    

    Use

    CRISPR; Fungal expression

    Tags

    SV40 NLS

    Expression

    Bacterial

    Mutation

    Promoter

    Aspergillus nidulans tef1 promoter and Aspergillu…

    Available since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. p2attB-FST344-HA-CPL

    Plasmid

    #131460

    Purpose

    Encodes human Follistatin protein

    Depositor

    Michele Calos

    Article

    Guha et al Mol Ther Methods Clin Dev. 2019 Oct 14;1

    Insert

    Follistatin (FST Human)
    

    Use

    Tags

    HA

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    May 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGMC00025 (aka pCW-dCas9-Blast)

    Plasmid

    #195316

    Purpose

    Dox inducible lentiviral dead Cas9

    Depositor

    Raj Chari

    Insert

    dead Cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    D10A; H840A

    Promoter

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEX-4T-1-3xFLAG-HSP27

    Plasmid

    #129229

    Purpose

    Encodes FLAG tagged human HSP27 for purification by GST affinity

    Depositor

    Kevin Janes

    Article

    Smolko et al Sci Rep. 2019 Dec 19;9(1):19409. doi: 10

    Insert

    HSP27 (HSPB1 Human)
    

    Use

    Tags

    FLAG and GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. Sth1Cas9n cytosine base editor

    Plasmid

    #194209

    Purpose

    Sth1Cas9-D9A-nickase_rAPOBEC1_cytosine base editor for RNA recording

    Depositor

    Chase Beisel

    Article

    Jiao et al Nat Biotechnol. 2023 Jan 5. doi: 10.1038

    Insert

    Sth1Cas9-D9A nickase
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Changed Aspartate 9 to Alanine.

    Promoter

    Available since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28a_TIA1

    Plasmid

    #106095

    Purpose

    bacterial protein expression of TIA1

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

    Insert

    TIA1 (TIA1 Human)
    

    Use

    Tags

    6xHIs-thrombin and T7

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKD068

    Plasmid

    #136471

    Purpose

    SSB-GFP IDL fusion expression plasmid

    Depositor

    James Keck

    Article

    Dubiel et al Nucleic Acids Res. 2020 Jun 19;48(11):60

    Insert

    SSB-GFP (ssb E. coli)
    

    Use

    Tags

    superfolder GFP

    Expression

    Bacterial

    Mutation

    superfolder GFP inserted between Phe148 and Ser149

    Promoter

    T7 promoter

    Available since

    April 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBDC

    Plasmid

    #135188

    Purpose

    Integration and excision of chloromaphenicol cassette for the E. coli genome via CreI, SceI, and lamba Red system. Contains SacB selection.

    Depositor

    Hongqing Fang

    Article

    Wei et al PLoS One. 2017 Oct 24;12(10):e0186891. d

    Insert

    CmR
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    None

    Promoter

    cat

    Available since

    March 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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