30,738 results

1. pUC19 PKC alpha

   Plasmid

   #8429

   Depositor

   Frederic Mushinski

   Insert

   Mouse Protein Kinase C alpha (Prkca Mouse)
   

   Expression

   Bacterial

   Available Since

   March 5, 2007

   Availability

   Academic Institutions and Nonprofits only

2. pOPINK-OTUD4 (OTU, aa 22-196)

   Plasmid

   #61413

   Purpose

   Expresses human OTUD4 (OTU domain) in E. coli.

   Depositor

   David Komander

   Article

   Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

   Insert

   OTUD4 (OTU domain-containing protein 4) (OTUD4 Human)
   

   Tags

   His6-GST-3C

   Expression

   Bacterial

   Mutation

   Deleted aa 1-21 and aa 197-1114.

   Promoter

   T7

   Available Since

   March 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAKlux2

   Plasmid

   #14080

   Depositor

   Attila Karsi

   Article

   Karsi et al Plasmid. 2007 May;57(3):286-95. doi: 10.

   Insert

   Bacterial luciferase
   

   Expression

   Bacterial

   Promoter

   Lac, T3

   Available Since

   March 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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4. MP28445 – pFUSE2ss-aRD114_CLIg-mIgK

   Plasmid

   #110556

   Purpose

   pFUSE backbone with an IL2 signal sequence, expressing the variable light chain of anti-RD114 binder fused to murine Kappa constant light chain.

   Depositor

   Martin Pule

   Article

   Mekkaoui et al J Gen Virol. 2019 Nov 8. doi: 10.1099/jg

   Insert

   anit-RD114 light chain
   

   Tags

   mKappa

   Expression

   Bacterial and Mammalian

   Promoter

   EF-1 alpha core promoter

   Available Since

   Aug. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. HSA2_His

   Plasmid

   #202764

   Purpose

   human serum albumin design HSA2, in pET29b with C-terminal 6His-tag

   Depositor

   Sarel Fleishman

   Article

   Khersonsky et al J Mol Biol. 2023 Sep 1;435(17):168191. d

   Insert

   HSA2
   

   Tags

   His tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSC101_TIMER

   Plasmid

   #103057

   Purpose

   low copy plasmid for expression of a variant of the fluorescent timer protein suitable for bacteria under the control of the constitutive ybaJp promoter (works in E. coli and Salmonella enterica)

   Depositor

   Dirk Bumann

   Article

   Claudi et al Cell. 2014 Aug 14;158(4):722-733. doi: 1

   Insert

   TIMER-bac
   

   Expression

   Bacterial

   Mutation

   mutations: S4T and S179T

   Promoter

   constitutive ybaJp promoter

   Available Since

   Nov. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHB-4

   Plasmid

   #140957

   Purpose

   For PHB production in E. coli (thrC3-pAC). Please note that this is listed as #140956 in the publication.

   Depositor

   Kang Zhou

   Article

   Liu et al Metab Eng. 2020 Sep;61:206-214. doi: 10.

   Insert

   phaCAB
   

   Expression

   Bacterial

   Available Since

   June 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. H2B-2A-GFP-IRESpuro2

   Plasmid

   #183746

   Purpose

   Expresses H2B-2A-GFP

   Depositor

   Daniel Gerlich

   Article

   Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

   Insert

   H2B (H2BC16P Human)
   

   Tags

   GFP with 2A protease cleavage site between H2B an…

   Expression

   Bacterial and Mammalian

   Promoter

   CMV

   Available Since

   July 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEX6P1-mCherry-anti-rabbit-IgG(Fc)-nanobody

   Plasmid

   #138131

   Purpose

   Expresses GST-tagged mCherry-anti-rabbit-IgG(Fc)-nanobody in E. coli cells.

   Depositor

   Kazuhisa Nakayama

   Article

   Katoh et al Mol Biol Cell. 2020 Jul 29:mbcE20040250.

   Insert

   mCherry-anti-rabbit-IgG(Fc)-nanobody
   

   Tags

   GST

   Expression

   Bacterial

   Available Since

   July 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGL4.R_TRE_minTK

    Plasmid

    #211516

    Purpose

    Dual-luciferase reporter plasmid backbone for insertion of your TRE of interest driving minTK-luc2P expression in addition to constitutive SV-40-driven Renilla luciferase expression

    Depositor

    Justin English

    Article

    Zahm et al bioRxiv. 2023 May 11:2023.05.11.539703.

    Type

    Empty backbone

    Expression

    Bacterial

    Promoter

    SV40 and minTK

    Available Since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBbA5c-MevT(CO)-T1-MBIS(CO, ispA)

    Plasmid

    #35152

    Depositor

    Jay Keasling, Taek Soon Lee

    Article

    Peralta-Yahya et al Nat Commun. 2011 Sep 27;2:483. doi: 10.1

    Insert

    AtoB(co)-HMGS(co)-HMGR(co)-pTrc-MK(co)-PMK(co)-PMD-Idi-IspA
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    R364S in Mevalonate Kinase

    Promoter

    LacUV5, pTrc

    Available Since

    May 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGRB

    Plasmid

    #71539

    Purpose

    for expression of gRNA in E. coli

    Depositor

    Tao Chen

    Article

    Li et al Metab Eng. 2015 Sep;31:13-21. doi: 10.10

    Type

    Empty backbone

    Expression

    Bacterial

    Promoter

    J231196

    Available Since

    Feb. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAB076i6

    Plasmid

    #79200

    Purpose

    Directed Evolution of Protein-Protein Interactions

    Depositor

    David Liu

    Article

    Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

    Insert

    PlacZ-opt (no operator) gIII, luxAB; Ppro1 434cI-SH2ABL1
    

    Expression

    Bacterial

    Promoter

    PlacZ-opt (no operator); Ppro1

    Available Since

    Aug. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTPH418b

    Plasmid

    #185725

    Purpose

    Directed evolution of Cas9 adenine base editors with altered PAM compatibility

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

    Insert

    gIII(N)-NpuN-NpuC-gIII(C)
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    Sept. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pACYC-TtCas6-4xcrRNA4.5

    Plasmid

    #127764

    Purpose

    For expression of T. thermophilus Csm complex in E. coli (crRNA)

    Depositor

    Jennifer Doudna

    Article

    Liu et al Nat Commun. 2019 Jul 5;10(1):3001. doi:

    Inserts

    Synthetic CRISPR array containing four identical T. thermophilus spacers
    Cas6A
    

    Expression

    Bacterial

    Mutation

    Codon optimized for expression in E. coli

    Promoter

    T7

    Available Since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTRK669

    Plasmid

    #71313

    Purpose

    Helper plasmid for pORI plasmids. Chloramphenicol resistant, temperature sensitive replication in E. coli and gram positives. Encodes RepA.

    Depositor

    Rodolphe Barrangou, Todd Klaenhammer

    Article

    Russell et al Appl Environ Microbiol. 2001 Sep;67(9):4

    Type

    Empty backbone

    Use

    E. coli-gram positive bacteria shuttle vector

    Expression

    Bacterial

    Available Since

    Jan. 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pQE30-His-PheRS

    Plasmid

    #124116

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Takuya Ueda, Sebastian Maerkl

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Phenylalanyl-tRNA ligase (pheS, pheT Escherichia coli)
    

    Tags

    6x-His

    Expression

    Bacterial

    Available Since

    June 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. EGFP-p300

    Plasmid

    #191764

    Purpose

    Expression of EGFP fused to core p300 histone acetyltransferase

    Depositor

    Daniel Gerlich

    Article

    Schneider et al Nature. 2022 Sep;609(7925):183-190. doi:

    Insert

    FRB-EGFP-p300 core (EP300 Human)
    

    Tags

    EGFP (N-terminal of p300 core) and FRB (N-termina…

    Expression

    Bacterial and Mammalian

    Promoter

    EF1alpha

    Available Since

    Feb. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSLQ14101 aeBlue

    Plasmid

    #239453

    Purpose

    express aeBlue chromoprotein

    Depositor

    Stanley Qi

    Article

    Collins et al Nat Commun. 2024 Aug 3;15(1):6563. doi:

    Insert

    aeBlue
    

    Use

    CRISPR; Cell-free system using bacterial extract

    Expression

    Bacterial

    Promoter

    J23119

    Available Since

    June 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. plRL61

    Plasmid

    #163633

    Purpose

    Optimized M. smegmatis CRISPRi plasmid; contains an L5 attP region (no L5 Integrase) that allows for integration when co-transformed with an L5 Integrase delivery plasmid (e.g. plRL19, ID #163634).

    Depositor

    Jeremy Rock

    Article

    Bosch et al Cell. 2021 Jul 16. pii: S0092-8674(21)00

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    Tet-On

    Available Since

    Aug. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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