29,712 results

1. pLN-AIDA-Tyr1

   Plasmid

   #192831

   Purpose

   IPTG inducible AIDA-Tyrosinase expression

   Depositor

   André Studart

   Article

   Binelli et al Adv Mater. 2022 Nov 29:e2207483. doi: 10

   Insert

   AIDA-Tyr1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pL-lac

   Available since

   Feb. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUD-MSP1D1

   Plasmid

   #161608

   Purpose

   Bacterial expression of mutated membrane scaffold protein ("ultra-dark" MSP1D1) for Nanodisc formation

   Depositor

   Stephen Sligar

   Article

   McLean et al Anal Biochem. 2020 Oct 15;607:113860. do

   Insert

   UD-MSP1D1
   

   Use

   Tags

   7-His tag

   Expression

   Bacterial

   Mutation

   mutant of MSP1D1; all tryptophans and tyrosines w…

   Promoter

   T7

   Available since

   Nov. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pVoPo-03

   Plasmid

   #187838

   Purpose

   Backbone for inducible expression system in Fusobacterium nucleatum

   Depositor

   Jörg Vogel

   Article

   Ponath et al Proc Natl Acad Sci U S A. 2022 Oct 4;119

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEX6P1‐hsRNASEH2BCA

   Plasmid

   #108692

   Purpose

   For expression in E coli of N-terminally GST-tagged human RNASEH2B and untagged RNASEH2C and A, allowing affinity purification of the RNase H2 trimeric enzyme

   Depositor

   Martin Reijns, Andrew Jackson

   Article

   Reijns et al J Biol Chem. 2011 Mar 25;286(12):10530-9

   Inserts

   RNASEH2B (RNASEH2B Human)
   RNASEH2C (RNASEH2C Human)
   RNASEH2A (RNASEH2A Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Shine Dalgarno sequence upstream of start codon a…

   Promoter

   tac

   Available since

   April 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pACYC-T7-SpCas9-T7-EGFP_gRNA1-T7term (BPK848)

   Plasmid

   #181745

   Purpose

   dual T7 promoter vector for bacterial expression of SpCas9 with a c-terminal NLS and 3xFLAG tag, along with an SpCas9 sgRNA targeting EGFP site 1

   Depositor

   Benjamin Kleinstiver

   Article

   Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038

   Insert

   human/zebrafish codon optimized SpCas9
   

   Use

   In vitro transcription; t7 promoter

   Tags

   NLS(SV40)-3xFLAG

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET-28a mScarlet

   Plasmid

   #178543

   Purpose

   The mScarlet fluorescent protein with an N-terminal 6xHis-tag in pET28a.

   Depositor

   Siegfried Musser

   Article

   Sharma et al Commun Biol. 2022 Sep 19;5(1):988. doi:

   Insert

   mScarlet
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. araBAD-BCD20-ClpP-BCD17-ClpX

   Plasmid

   #153301

   Purpose

   Expresses clpX and clpP in E.Coli

   Depositor

   Mikhail Shapiro

   Article

   Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. d

   Inserts

   clpX (clpX Escherichia coli)
   clpP (clpP Escherichia coli)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   araBAD

   Available since

   Sept. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAB1

   Plasmid

   #62547

   Purpose

   broad host range plasmid for IPTG-inducible expression of eGFP. Designed for imaging Pseudomonas aeruginosa activity in biofilms.

   Depositor

   Michael Franklin

   Article

   Walters MC et al Antimicrob Agents Chemother. 2003 Jan;47

   Insert

   eGFP
   

   Use

   Broad host range plasmid

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pTrc

   Available since

   April 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKD236-4b

   Plasmid

   #90956

   Purpose

   Expresses ThsS constitutively

   Depositor

   Jeffrey Tabor

   Article

   Daeffler et al Mol Syst Biol. 2017 Apr 3;13(4):923. doi

   Insert

   ThsS
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Bba_J23104

   Available since

   May 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. FLAG-Cdk9

    Plasmid

    #28100

    Depositor

    Andrew Rice

    Article

    Wang et al J Biol Chem. 2008 Nov 28. 283(48):33578-

    Insert

    Cdk9 (CDK9 Human)
    

    Use

    Tags

    FLAG

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    March 15, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTHSSe_49

    Plasmid

    #109247

    Purpose

    pSC101 var7 BbsI cloning vector

    Depositor

    Christopher Voigt

    Article

    Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PJ23105

    Available since

    Jan. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTD-CTwinStrep

    Plasmid

    #45939

    Purpose

    use to create C-terminal Twin-Strep-tag fusion proteins

    Depositor

    Thorben Dammeyer

    Article

    Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.

    Type

    Empty backbone

    Use

    Tags

    Twin-Strep

    Expression

    Bacterial

    Mutation

    Promoter

    Trc (lactose/IPTG inducible)

    Available since

    Sept. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. p2B-55

    Plasmid

    #177924

    Purpose

    Expresses optimized unleaky reverse tetracycline transactivator (rtTA-M2-SE-G72P) under control of yeast TDH3 promoter

    Depositor

    Vadim Gladyshev

    Article

    Barre et al (unpublished)

    Insert

    Optimized unleaky reverse tetracycline transactivator (rtTA-M2-SE-G72P) under control of yeast TDH3 promoter
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    V9I; F67S; G72P, F86Y; R171K

    Promoter

    TDH3

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMPMA3dPlac-PprgH-GFPLVA

    Plasmid

    #23340

    Depositor

    Olivia Steele-Mortimer

    Article

    Ibarra et al Microbiology. 2009 Dec 24. ():.

    Insert

    GFP(LVA)
    

    Use

    Tags

    GFP(LVA)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 27, 2010

    Availability

    Academic Institutions and Nonprofits only

15. His6-SpyCatcher-mi3

    Plasmid

    #113043

    Purpose

    Expresses His6-SpyCatcher-mi3 in bacterial cells as platform for plug-and-display vaccination

    Depositor

    Mark Howarth

    Article

    Bruun et al ACS Nano. 2018 Jul 20. doi: 10.1021/acsn

    Insert

    His6-SpyCatcher-mi3
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBS cPitx2 (CT#73)

    Plasmid

    #13927

    Depositor

    Cliff Tabin

    Article

    Logan et al Cell. 1998 Aug 7. 94(3):307-17.

    Insert

    pitx2 (PITX2 Chicken)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 9, 2007

    Availability

    Academic Institutions and Nonprofits only

17. pET28a SpyCatcher-SnoopCatcher

    Plasmid

    #72324

    Purpose

    Bacterial expression of SpyCatcher linked to SnoopCatcher.

    Depositor

    Mark Howarth

    Article

    Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p

    Insert

    pET28a SpyCatcher-SnoopCatcher
    

    Use

    Tags

    N-terminal His6 tag

    Expression

    Bacterial

    Mutation

    35 amino acids at the N-term of SpyCatcher have b…

    Promoter

    Available since

    Feb. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBEST-OR2-OR1-Pr-araC, pBAD-TetR, pBAD-TetO1-deGFP-ssrA

    Plasmid

    #45789

    Depositor

    Richard Murray

    Inserts

    deGFP
    Tetracycline repressor
    araC
    

    Use

    Synthetic Biology

    Tags

    ssrA degradation tag

    Expression

    Bacterial

    Mutation

    Promoter

    OR2-OR1-Pr (bacteriophage Lambda with one mutatio…

    Available since

    April 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET-Trx-mSA2

    Plasmid

    #52320

    Purpose

    Solubly expresses monomeric streptavidin containing S25H mutation in E. coli as a thioredoxin fusion. App Microbiol & Biotech 98, 6285-6295 (2014)

    Depositor

    Sheldon Park

    Article

    Demonte et al Appl Microbiol Biotechnol. 2014;98(14):6

    Insert

    monomeric streptavidin 2
    

    Use

    Tags

    FLAG, His, S tag, TEV site, and Thioredoxin

    Expression

    Bacterial

    Mutation

    S25H mutation compared to streptavidin

    Promoter

    T7

    Available since

    July 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUCP20T-mPlum

    Plasmid

    #78474

    Purpose

    Broad range bacterial fluorescence labeling

    Depositor

    Mariette Barbier

    Article

    Barbier et al PLoS One. 2016 Mar 3;11(3):e0146827. doi

    Insert

    mPlum
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge