30,738 results

1. HMBP-3C-STAR.66-285

   Plasmid

   #100094

   Purpose

   expresses MBP-tagged STARD1 protein domain (residues 66-285)

   Depositor

   Nikolai Sluchanko

   Article

   Sluchanko et al Protein Expr Purif. 2016 Mar;119:27-35.

   Insert

   STAR protein (STAR Human)
   

   Tags

   His-tagged MBP

   Expression

   Bacterial

   Promoter

   Ptac

   Available Since

   Sept. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pETSXM2-pLacI-mtrC

   Plasmid

   #174615

   Purpose

   Bacterial expression of mtrC from Shewanella oneidensis MR-1.

   Depositor

   I-Son Ng

   Article

   I-Son Ng et al Bioresour. Bioprocess. 5, 35 (2018)

   Insert

   MtrC
   

   Use

   Synthetic Biology; Shuttle vector in e. coli and …

   Expression

   Bacterial

   Promoter

   pLacI

   Available Since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pConRef-2H12.D11

   Plasmid

   #221151

   Purpose

   cdGreen2 expressed from a constitutive promoter in an operon with mScarlet-I (downstream) as a reference FP Internal Lab ID: 11236)

   Depositor

   Urs Jenal

   Article

   Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

   Inserts

   cdGreen2
   mScarlet-I
   

   Expression

   Bacterial

   Mutation

   N-terminus changed: starts with MSKKYGEAVIKE ...

   Promoter

   Constitutive and None (same as cdGreen2)

   Available Since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRK172-6xHis-0N4R-P301S-tau-HiBiT

   Plasmid

   #187835

   Purpose

   Expression of 6xHis-tagged P301S tau of 0N4R isoform with a C-terminal HiBiT tag for bimolecular luminescence with LgBiT.

   Depositor

   William McEwan

   Article

   Tuck et al Cell Rep. 2022 May 3;39(5):110776. doi:

   Insert

   6XHis-0N4R-P301S-tau-HiBiT
   

   Tags

   6xHistidine and HiBiT

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAT-WT-Myc

   Plasmid

   #177717

   Purpose

   ER protein alpha-1-antitrypsin (WT), myc tagged

   Depositor

   Erik Böttger

   Article

   Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

   Insert

   alpha-1-antitrypsin (SERPINA1 Human)
   

   Tags

   Myc

   Expression

   Bacterial and Mammalian

   Available Since

   Jan. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRBBm34

   Plasmid

   #48114

   Purpose

   Expression of the gfp gene under control of the native xylose inducible promoter of Bacillus megaterium

   Depositor

   Dieter Jahn

   Article

   Biedendieck et al J Biotechnol. 2007 Dec 1;132(4):426-30.

   Insert

   gfp
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Oct. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRSF-G1(4/5FTrp)RS

   Plasmid

   #207620

   Purpose

   tRNA synthetase/tRNA pair for the in vivo incorporation of 4-Fluoro-L-Tryptophan (4FTrp) and 5-Fluoro-L-Tryptophan (5FTrp) into proteins in E. coli in response to the amber (TAG) codon

   Depositor

   Thomas Huber

   Article

   Qianzhu et al J Am Chem Soc. 2024 Apr 30. doi: 10.1021

   Insert

   tRNA synthetase
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSUMO2

   Plasmid

   #52259

   Purpose

   Bacterial expression of human 6His-tagged SUMO2, SUMO-E1 and SUMO-E2

   Depositor

   Primo Schaer

   Article

   Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

   Inserts

   SUMO-2 (SUMO2 Human)
   UBE2l (UBE2I Human)
   SAE-2 (UBA2 Human)
   SAE-1 (SAE1 Human)
   

   Tags

   6His-tag

   Expression

   Bacterial

   Mutation

   C-terminal gly-gly

   Promoter

   T7 and rbs

   Available Since

   July 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28a-mH6-Cas12i1

   Plasmid

   #120882

   Purpose

   Expresses E. coli codon optimized Cas12i1 effector in the pET28a backbone.

   Depositor

   Arbor Biotechnologies

   Article

   Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

   Insert

   Cas12i1
   

   Use

   CRISPR

   Tags

   mH6

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   lac

   Available Since

   Feb. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSF18

    Plasmid

    #222593

    Purpose

    BB2 ZWF1 tet-on overexpression cassette

    Depositor

    Matthias Steiger

    Article

    Matthias et al PLOS One

    Insert

    ZWF1
    

    Expression

    Bacterial

    Mutation

    Internal BsaI and BpiI sites removed from insert

    Promoter

    tet-on

    Available Since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLyGo-Bs-1

    Plasmid

    #163137

    Purpose

    pLyGo cloning vector for a sequence of interest (LPMO) in Bacillus subtilis. Vector encoding the AmyL signal peptide and the LyGo casette (SapI-ccdB-SapI)

    Depositor

    Morten Norholm

    Article

    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    PamyL-PamyQ-PcryIIIA-cryIIIAstab49

    Available Since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pACYCDuet1-tglB

    Plasmid

    #134676

    Purpose

    Expresses His6-TglBin E. Coli

    Depositor

    Wilfred van der Donk

    Article

    Ting et al Science. 2019 Jul 19;365(6450):280-284.

    Insert

    TglB
    

    Tags

    His6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. p3036 GST-HPV-16 E6

    Plasmid

    #10849

    Depositor

    Peter Howley

    Article

    Huibregtse et al EMBO J. 1991 Dec . 10(13):4129-35.

    Insert

    HPV16 E6 (E6 Human papillomavirus)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    May 24, 2006

    Availability

    Academic Institutions and Nonprofits only

14. pTHSSe_60

    Plasmid

    #109254

    Purpose

    TALEsp2 stabilized promoter driving GFP

    Depositor

    Christopher Voigt

    Article

    Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

    Insert

    sfGFP
    

    Expression

    Bacterial

    Promoter

    TALEsp2 stabilized promoter

    Available Since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAT203

    Plasmid

    #134880

    Purpose

    Bacterial expression

    Depositor

    Thomas Howard

    Article

    Azubuike et al N Biotechnol. 2021 Nov 25;65:20-30. doi:

    Insert

    mRFP1
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    July 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDMS197

    Plasmid

    #43831

    Depositor

    Dieter Schifferli

    Article

    Edwards et al Gene. 1998 Jan 30;207(2):149-57.

    Type

    Empty backbone

    Use

    Bacterial allelic exchange vector with sacb1

    Expression

    Bacterial

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTriEx-RhoA-wt_mScarlet-i_SGFP2

    Plasmid

    #85071

    Purpose

    Green/red FRET biosensor to monitor RhoA nucleotide loading state and activity

    Depositor

    Dorus Gadella

    Article

    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

    Insert

    RhoA Biosensor wildtype with mScarlet-i and SGFP2 (RHOA Human)
    

    Tags

    6xHis and mScarlet-i and SGFP2

    Expression

    Bacterial, Insect, and Mamm…

    Promoter

    CMV

    Available Since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET-Himar-dCas9

    Plasmid

    #137079

    Purpose

    Himar-dCas9 gene with C-terminal 6x-His tag for protein purification

    Depositor

    Harris Wang

    Article

    Chen et al CRISPR J. 2019 Dec;2(6):376-394. doi: 10

    Insert

    Himar-dCas9
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAT28-proD/SaEGFP

    Plasmid

    #120321

    Purpose

    Bacterial eGFP labeling

    Depositor

    James Kirby

    Article

    Kang et al Infect Immun. 2017 Apr 21;85(5). pii: e0

    Insert

    eGFP with Staphylococcus aureus codon optimization
    

    Expression

    Bacterial

    Promoter

    proD

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBbA6c_PsulA_sfGFP

    Plasmid

    #224587

    Purpose

    SOS response reporter

    Depositor

    Ophelia Venturelli

    Article

    Cheng et al Mol Syst Biol. 2023 Mar 9;19(3):e11406.

    Insert

    sfGFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    PsulA

    Available Since

    Oct. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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