30,416 results
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Plasmid#228663PurposeBacterial co-expression of the NS2B and NS3 proteases from Zika virus for crystallization assays in P43 2 2 (PDB:8PN6)DepositorInsertZika virus portion of NS2B protease coexpressed with NS3 protease
UseTagsHis-GST-TEVExpressionBacterialMutationPromoterAvailable SinceFeb. 18, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET28:LanIII
Plasmid#225075PurposeExpresses precursor peptide and lanthipeptide synthetase from LanIII (FAST-RiPPs) in E. coliDepositorInsertLanA (LanIII)
UseTagsHis6ExpressionBacterialMutationWTPromoterT7Available SinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28:LanIII-43
Plasmid#225077PurposeExpresses precursor peptide and lanthipeptide synthetase from LanIII-43 (FAST-RiPPs) in E. coliDepositorInsertLanA (LanIII-43)
UseTagsHis6ExpressionBacterialMutationWTPromoterT7Available SinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28:LanII
Plasmid#225066PurposeExpresses precursor peptide and lanthipeptide synthetase from LanII (FAST-RiPPs) in E. coliDepositorInsertLanA (LanII)
UseTagsHis6ExpressionBacterialMutationWTPromoterT7Available SinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDM068
Plasmid#216811PurposeVector for Aspergillus CRISPR-Cas9 genetic engineering with Golden Gate cloning drop-out cassette for protospacer insertion and NAT selectable marker.DepositorTypeEmpty backboneUseCRISPR; Fungal expressionTagsExpressionBacterialMutationPromoterAvailable SinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDM053
Plasmid#216815PurposeAspergillus nidulans codon-adjusted mNeonGreen fluorescent protein, includes linker for N-terminal tagging.DepositorInsertmNeonGreen
UseTagsRIPGLINExpressionBacterialMutationAspergillus nidulans codon-adjustedPromoterAvailable SinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDM071
Plasmid#216817PurposeAspergillus nidulans codon-adjusted mCardinal fluorescent protein, includes linker for N-terminal tagging.DepositorInsertmCardinal
UseTagsRIPGLINExpressionBacterialMutationAspergillus nidulans codon-adjustedPromoterAvailable SinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
human KANK2 Ankyrin Repeats pET151
Plasmid#164054PurposeExpresses human KANK2 Ank domain in bacterial cellsDepositorInsertKidney Ankyrin Repeat-Containing Protein 2 (KANK2 Human)
UseTagsHis-tag, TEV cleavage siteExpressionBacterialMutationPromoterT7Available SinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
HP4_M13
Plasmid#120340PurposeHelper plasmid for production of phagemid ssDNA, based on m13mp18DepositorInsertCoding part of the M13 phage genome
UseTagsExpressionBacterialMutationPromoternative phage promotersAvailable SinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCali2
Plasmid#86119PurposeMultiplex temperature-gated expression of mWasabi and mCherry reportersDepositorInsertsTlpA36
mWasabi
TcI
mCherry
UseTags6xHisExpressionBacterialMutationA67T and D341A, P60L, D135V, K187R, K202I, L208QPromoterpR/pL, pR/pL, pLacI, pTlpA, and pTlpA, pLacIAvailable SinceFeb. 27, 2017AvailabilityAcademic Institutions and Nonprofits only