30,738 results

1. pGDP2 MCR-1

   Plasmid

   #118404

   Purpose

   This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the pLac promoter.

   Depositor

   Gerard Wright

   Article

   Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

   Insert

   MCR-1
   

   Expression

   Bacterial

   Promoter

   pLac

   Available Since

   Dec. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. plRL118

   Plasmid

   #163632

   Purpose

   Renilla luciferase reporter plasmid; Giles integrating.

   Depositor

   Jeremy Rock

   Article

   Bosch et al Cell. 2021 Jul 16. pii: S0092-8674(21)00

   Type

   Empty backbone

   Use

   Luciferase

   Expression

   Bacterial

   Promoter

   Mycobacterial Optimized Promoter (MOP)

   Available Since

   Aug. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pATetO 6XHis-Taq DNA Pol

   Plasmid

   #178782

   Purpose

   Inducible expression of Taq DNA polymerase in E. coli

   Depositor

   Andrew Ellington

   Article

   Bhadra et al PLoS One. 2021 Jun 1;16(6):e0252507. doi

   Insert

   Taq DNA Polymerase
   

   Expression

   Bacterial

   Available Since

   March 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET-45b-POLR2A-CTD-mCherry

   Plasmid

   #185011

   Purpose

   Bacterial Expression of 6xHis C-terminal domain of POLR2A mCherry Fusion Protein

   Depositor

   Denes Hnisz

   Article

   Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

   Insert

   POLR2A (Polr2a Mouse)
   

   Expression

   Bacterial

   Available Since

   June 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET51b(+)_HaloTag9

   Plasmid

   #169324

   Purpose

   Expression of HaloTag9 in bacterial cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   HaloTag9
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   HaloTag9 = HaloTag7-Q165H-P174R

   Available Since

   Nov. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. iYFP-Parkin Plasmid

   Plasmid

   #197107

   Purpose

   Expresses YFP-tagged Parkin when induced by Doxycycline in mammalian cells.

   Depositor

   Dori Woods

   Article

   Capelluto et al Adv Biol (Weinh). 2023 Jan 18:e2200246.

   Insert

   parkinson protein 2, E3 ubiquitin protein ligase (PRKN Human)
   

   Use

   Retroviral

   Tags

   YFP

   Expression

   Bacterial and Mammalian

   Available Since

   May 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET21-pelB-LaG94-5

   Plasmid

   #228965

   Purpose

   For bacterial expression of anti-GFP nanobody LaG94-5, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

   Insert

   LaG94-5
   

   Tags

   6xHIS

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDT-SuperFi-Cas9

   Plasmid

   #183630

   Purpose

   Expression and purification of N-terminal His-MBP tagged SuperFi Cas9 in E. coli

   Depositor

   David Taylor

   Article

   Bravo et al Nature. 2022 Mar 2. pii: 10.1038/s41586-

   Insert

   SuperFi-Cas9
   

   Use

   CRISPR

   Tags

   6xHis-MBP

   Expression

   Bacterial

   Mutation

   Y1010D, Y1013D, Y1016D, V1018D, R1019D, Q1027D, …

   Promoter

   T7

   Available Since

   May 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. p6272 pDEST-60-GST-HPV16E6

   Plasmid

   #24127

   Depositor

   Peter Howley

   Insert

   HPV16 E6 (E6, E6 HPV)
   

   Expression

   Bacterial and Mammalian

   Mutation

   none

   Available Since

   April 2, 2010

   Availability

   Academic Institutions and Nonprofits only

10. pC018 - LshCas13a from Leptotrichia shahii

    Plasmid

    #91908

    Purpose

    Expresses LshCas13a for bacterial expression and contains backbone for spacer cloning

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

    Insert

    LshCas13a and guide expression cassette
    

    Expression

    Bacterial

    Available Since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMyrPalm_mEGFP

    Plasmid

    #21037

    Depositor

    Daniel Gerlich

    Article

    Steigemann et al Cell. 2009 Feb 6. 136(3):473-84.

    Insert

    pMyrPalm
    

    Tags

    mEPFP

    Expression

    Bacterial and Mammalian

    Available Since

    May 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSpyC-GFP

    Plasmid

    #220191

    Purpose

    sgRNA expression vector - pBG42 promoter with GFP in sgRNA site

    Depositor

    Larry Halverson

    Article

    Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

    Insert

    pBG42 promoter with GFP in sgRNA site
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    monomeric superfolder green fluorescent protein

    Promoter

    pBG42

    Available Since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMRS-LacI-dCas9

    Plasmid

    #220194

    Purpose

    mini-Tn7 dCas9 expression vector - LacI/pLac promoter

    Depositor

    Larry Halverson

    Article

    Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

    Insert

    Pseudomonas aeruginosa-codon optimized Streptococcus pyogenes dCas9
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    Pseudomonas aeruginosa codon optimized

    Promoter

    LacI/pLac

    Available Since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCPP5372

    Plasmid

    #128733

    Purpose

    Gateway destination low-copy number plasmid for expression of any effector from avrPto promoter with C-terminal HA tag

    Depositor

    Alan Collmer

    Article

    Oh et al J Bacteriol. 2007 Nov;189(22):8277-89. E

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Jan. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pB1H2w2-zif268

    Plasmid

    #18045

    Depositor

    Scot Wolfe

    Article

    Noyes et al Nucleic Acids Res. 2008 May;36(8):2547-6

    Insert

    zif268
    

    Expression

    Bacterial

    Mutation

    Zif268 is fused to the omega subunit of RNA polym…

    Available Since

    June 20, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJExpress414_GmPOPB-S577A

    Plasmid

    #92235

    Purpose

    Express S577A mutant of prolyl oligopeptidase from Galerina marginata

    Depositor

    James Naismith

    Article

    Czekster et al Biochemistry. 2017 Apr 18;56(15):2086-20

    Insert

    Prolyl oligopeptidase B from Galerina marginata-S577A mutant
    

    Tags

    6x-Histag, TEV cleavage site

    Expression

    Bacterial

    Mutation

    Serine 577 to Alanine

    Promoter

    T7 promoter

    Available Since

    Aug. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKL10-mRuby-iATPSnFR1.0 bb118

    Plasmid

    #158980

    Purpose

    Constitutive expression (sigma 70 promoter) of the ratiometric mRuby-iATPSnFR1.0 ATP indicator in E. coli.

    Depositor

    Joel Kralj

    Article

    Bruni et al eLife. 2020 Aug 4;9. pii: 58706. doi: 10

    Insert

    mruby-iATPSNfR1.0
    

    Tags

    mruby3

    Expression

    Bacterial

    Mutation

    codon optimized for e. coli from Addgene#102557

    Promoter

    Bba_J23118 (sigma 70)

    Available Since

    Sept. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCT310

    Plasmid

    #111140

    Purpose

    Bacterial expression of 6His-dCas9-3XFlag

    Depositor

    Robert Tjian

    Article

    Tsui et al Proc Natl Acad Sci U S A. 2018 Mar 20;11

    Insert

    dCas9
    

    Tags

    3X Flag and 6X His

    Expression

    Bacterial

    Mutation

    D10A - H840A

    Promoter

    T7

    Available Since

    July 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. Cys-30nmSAH-SPYcatcher

    Plasmid

    #214839

    Purpose

    This plasmid encodes a protein (Cys-30nmSAH-SPYcatcher) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.

    Depositor

    Hironori Funabiki

    Article

    Arimura et al eLife. 2025 May 20;13:RP103486. doi: 10.

    Insert

    Cys-30nmSAH-SPYcatcher
    

    Tags

    His-TEV-SUMO-

    Expression

    Bacterial

    Promoter

    T5

    Available Since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDawn-Ag43

    Plasmid

    #107742

    Purpose

    Expresses Ag43 under the control of a blue-light transcriptional regulator

    Depositor

    Ingmar Riedel-Kruse

    Article

    Jin et al Proc Natl Acad Sci U S A. 2018 Apr 3;115

    Insert

    Ag43 (flu )
    

    Expression

    Bacterial

    Mutation

    removed all pstI cut sites with silent mutations,…

    Promoter

    pLambda

    Available Since

    April 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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