29,712 results

1. pET-SisTnpB1–ωRNA

   Plasmid

   #228365

   Purpose

   Expresses SisTnpB1 and RNP complex in E. coli Rosetta (DE3) cells

   Depositor

   Tao Liu

   Article

   Xu et al Commun Biol. 2024 Nov 20;7(1):1541. doi:

   Insert

   SisTnpB1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAGM22082_sfGFP1-10

   Plasmid

   #153515

   Purpose

   E.coli expression plasmid for the production of GFP1-10

   Depositor

   Sylvestre Marillonnet

   Article

   Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

   Insert

   sfGFP1-10
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBbS9a-RFP

   Plasmid

   #126172

   Purpose

   Constitutive expression vector for Escherichia coli. BglBrick compatible.

   Depositor

   Nigel Scrutton

   Article

   Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

   Insert

   RFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23113

   Available since

   June 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET28-SaCas9

   Plasmid

   #178901

   Purpose

   SaCas9 (Cas9 from Staphylococcus aureus)

   Depositor

   Hendrik Milting

   Article

   Flottmann et al STAR Protoc. 2022 Apr 5;3(2):101276. doi

   Insert

   SaCas9
   

   Use

   Tags

   His6-Tag, bipartite nuclear localization signal f…

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGEx-4T-1-YTHDF1

   Plasmid

   #70087

   Purpose

   Encode YTHDF1 with N-terminal GST tag for E. coli expression

   Depositor

   Chuan He

   Article

   Wang et al Nature. 2014 Jan 2;505(7481):117-20. doi

   Insert

   YTH N(6)-methyladenosine RNA binding protein 1 (YTHDF1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Nov. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Ubiquitin (1-76; T66V, L67N)

   Plasmid

   #110755

   Purpose

   Bacterial expression of human Ubiquitin (1-76; T66V, L67N)

   Depositor

   David Komander

   Article

   Gladkova et al EMBO J. 2017 Dec 15;36(24):3555-3572. do

   Insert

   UBB (UBB Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   T66V, L67N

   Promoter

   T7

   Available since

   Aug. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pYTK072

   Plasmid

   #65179

   Purpose

   Encodes ConRE as a Type 5 part to be used in the Dueber YTK system

   Depositor

   John Dueber

   Article

   Lee et al ACS Synth Biol. 2015 Apr 14.

   Insert

   ConRE
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. CD Ugi1

   Plasmid

   #177194

   Purpose

   Greengate entry vector pGGC000 modified to contain bladderwort insulator 1

   Depositor

   Wayne Parrott

   Article

   Park et al bioRxiv 2021.11.04.467280

   Insert

   Utricularia gibba insulator 1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 28, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. pProEx N-Ras(G12C) CL

   Plasmid

   #224262

   Purpose

   Protein purification of N-Ras (G12C)

   Depositor

   Kevan Shokat

   Article

   Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

   Insert

   N-Ras (NRAS Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   G12C

   Promoter

   Available since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pProEx H-Ras(G12C) CL

    Plasmid

    #224261

    Purpose

    Protein purification of H-Ras (G12C)

    Depositor

    Kevan Shokat

    Article

    Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

    Insert

    H-Ras (HRAS Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    G12C

    Promoter

    Available since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pTCF5 HLA-A*01:01

    Plasmid

    #180448

    Purpose

    E. coli expression of soluble MHC protein

    Depositor

    John Altman

    Insert

    HLA-A*01:01
    

    Use

    Tags

    BSP41

    Expression

    Bacterial

    Mutation

    Replacement of transmembrane domain with enzymati…

    Promoter

    Available since

    Feb. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. RNAPII_CTD27-52

    Plasmid

    #98678

    Purpose

    Expresses 6xHis-tagged C-terminal domain of RNA polymerase II

    Depositor

    Nicolas Fawzi

    Article

    Janke et al Biochemistry. 2017 Oct 11. doi: 10.1021/

    Insert

    RNA polymerase II C-terminal domain (POLR2A Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pZ8-Ptac

    Plasmid

    #74064

    Purpose

    IPTG inducible version of pZ8-1 plasmid, to which lacIq was added, KanR

    Depositor

    Timothy Lu

    Article

    Cleto et al ACS Synth Biol. 2016 Feb 16.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    ptac

    Available since

    April 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pVoPo-mNG

    Plasmid

    #187843

    Purpose

    Constitutive mNeonGreen-expression vector for Fusobacterium nucleatum

    Depositor

    Jörg Vogel

    Article

    Ponath et al Proc Natl Acad Sci U S A. 2022 Oct 4;119

    Insert

    mNeonGreen
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGP-RSET-iGABASnFR2-6xHis

    Plasmid

    #218885

    Purpose

    Bacterial expression of improved GABA sensor (positive change in fluorescence)

    Depositor

    GENIE Project

    Article

    Kolb et al bioRxiv 2025.03.25.644953

    Insert

    iGABASnFR2
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    S99A F102Y F104Y L178S

    Promoter

    T7

    Available since

    May 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. His-tagged FL Rec8

    Plasmid

    #45243

    Purpose

    raise antibody against Rec8

    Depositor

    Michael Lampson

    Article

    Chiang et al Curr Biol. 2010 Sep 14;20(17):1522-8. do

    Insert

    Rec8 (Rec8 Mouse)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGEX-human p53-(1-393)

    Plasmid

    #24860

    Depositor

    Cheryl Arrowsmith

    Article

    Ayed et al Nat Struct Biol. 2001 Sep . 8(9):756-60.

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pETM33_Nsp15

    Plasmid

    #156481

    Purpose

    Bacterial expression of Sars-CoV2 Nsp15 protein with His-tag and GST-tag

    Depositor

    Ylva Ivarsson

    Article

    Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

    Insert

    Nsp15 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
    

    Use

    Tags

    His-GST

    Expression

    Bacterial

    Mutation

    Gene insert is codon optimized for Ecoli.

    Promoter

    T7/LacO

    Available since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRK172-α-syn-TEV-GFP

    Plasmid

    #166671

    Purpose

    Bacterial expression of alpha synuclein coupled to GFP via a TEV site for uptake studies

    Depositor

    Iris Lindberg

    Article

    Jarvela et al PLoS One. 2021 Jan 26;16(1):e0241161. do

    Insert

    mouse synuclein (from V. Lee) (Snca Mouse)
    

    Use

    Tags

    GFP

    Expression

    Bacterial

    Mutation

    added a TEV cleavage site sequence between synucl…

    Promoter

    CMV

    Available since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSC5GGv1

    Plasmid

    #194645

    Purpose

    pSC5 Golden-Gate Cloning Plasmid (BsaI - Site 1)

    Depositor

    Bogumil Karas

    Article

    Cochrane et al Biodes Res. 2022 Aug 19;2022:9802168. do

    Type

    Empty backbone

    Use

    Synthetic Biology; Diatom expression

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge