29,729 results

1. GST TopBP1 (aa 32-1522) His

   Plasmid

   #20375

   Depositor

   Aziz Sancar

   Article

   Choi et al Nucleic Acids Res. 2009 Jan 12. ():.

   Insert

   TopBP1 (TOPBP1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Starts at aa32

   Promoter

   Available since

   March 5, 2009

   Availability

   Academic Institutions and Nonprofits only

2. pDEST17-UbE2B

   Plasmid

   #15781

   Depositor

   Wade Harper

   Article

   Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

   Insert

   Ubiquitin conjugating enzyme UbE2B (UBE2B Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 28, 2007

   Availability

   Academic Institutions and Nonprofits only

3. pET24b-SUMO-BG2.4

   Plasmid

   #199438

   Purpose

   expresses BAmGreen2.4 in bacterial cells with a SUMO tag

   Depositor

   Andrew Woolley

   Article

   Jang et al Nat Methods. 2023 Feb 23. doi: 10.1038/s

   Insert

   BAMGreen2.4
   

   Use

   Tags

   his tag and sumo

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDR111_mannose_Bgal no NLS

   Plasmid

   #188399

   Purpose

   Expresses beta-galactosidase by D-mannose promoter for Bacillus subtilis. There is a secretion peptide (PhoD) that is fused to beta-galactosidase.

   Depositor

   Christopher Contag

   Article

   Madsen et al Commun Biol. 2022 Aug 30;5(1):888. doi:

   Insert

   lacZ
   

   Use

   Tags

   PhoD secretion peptide

   Expression

   Bacterial

   Mutation

   Promoter

   D-mannose

   Available since

   Aug. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. MSP573

   Plasmid

   #98796

   Purpose

   Expression Vector

   Depositor

   David Liu

   Article

   Packer et al Nat Commun. 2017 Oct 16;8(1):956. doi: 1

   Insert

   pET28 MBP-ENLYFQS-TEV WT
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   None

   Promoter

   Available since

   Oct. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pKCyR8

   Plasmid

   #149494

   Purpose

   Broad-Host range expression vector, RK2 and pUC origin, CymR(AM) repressor, PCymRC promoter, mRFP, Hygromycin Resistance Marker.

   Depositor

   Christopher Reisch

   Article

   Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

   Insert

   CymR(AM)-mRFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PCymRC

   Available since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pKD237-3a-2

   Plasmid

   #90957

   Purpose

   Expresses ThsR and mCherry constitutively and sfGFP under the PphsA promoter

   Depositor

   Jeffrey Tabor

   Article

   Daeffler et al Mol Syst Biol. 2017 Apr 3;13(4):923. doi

   Inserts

   Thiosulfate response regulator
   Superfolder GFP
   mCherry
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Bba_J23100, Bba_J23105, and PphsA

   Available since

   May 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBK- Ma PylRS WT

   Plasmid

   #197573

   Purpose

   Expresses wild-type Methanomethylophilus alvus pyrrolysine tRNA synthetase under GlnS promoter. Used for Pyl tRNA-synthetase selections.

   Depositor

   Ryan Mehl

   Article

   Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

   Insert

   M. alvus PylRS WT
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   GlnS (constitutive)

   Available since

   March 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET 28 full-length human PARP1

   Plasmid

   #192635

   Purpose

   Expresses human PARP1 with an N-terminal His-tag

   Depositor

   John Pascal

   Article

   Langelier et al J Biol Chem. 2008 Feb 15;283(7):4105-14.

   Insert

   Human PARP1 (PARP1 Human)
   

   Use

   Tags

   histidine-tag

   Expression

   Bacterial

   Mutation

   Polymorphism A762

   Promoter

   Available since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28-3'TwStrep

    Plasmid

    #208053

    Purpose

    Empty vector for prokaryotic expression, insert a Twin Strep tag (with TAA) between HindII and XhoI site of pET28a (+), both HindIII and XhoI are retained. HindIII in-frame with Twin Strep tag.

    Depositor

    Quanfu Ma

    Type

    Empty backbone

    Use

    Tags

    Twin Strep

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. BPTF PHD and Bromodomain

    Plasmid

    #92101

    Purpose

    Expresses GST fusion PHD and Bromodomain from BPTF protein

    Depositor

    Albert Jeltsch

    Article

    Mauser et al Biochimie. 2018 Mar;146:14-19. doi: 10.1

    Insert

    PHD and Bromodomain (BPTF Human)
    

    Use

    Tags

    GST Tag

    Expression

    Bacterial

    Mutation

    Promoter

    tac promoter

    Available since

    June 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET23b-Casp9-His

    Plasmid

    #11829

    Depositor

    Guy Salvesen

    Article

    Stennicke et al J Biol Chem. 1999 Mar 26. 274(13):8359-6

    Insert

    Caspase 9 (CASP9 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 12, 2006

    Availability

    Academic Institutions and Nonprofits only

13. pET28_mouse-pro-IL1B

    Plasmid

    #183386

    Purpose

    Overexpression of catalytic domain of inflammatory caspase in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

    Insert

    pro-IL1B (Il1b Mouse)
    

    Use

    Tags

    His-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. GST-PRMT7

    Plasmid

    #34693

    Depositor

    Steven Clarke

    Article

    Miranda et al J Biol Chem. 2004 May 28;279(22):22902-7

    Insert

    PRMT7 (PRMT7 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Jan. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

15. pT7C-HSP90AB1

    Plasmid

    #177659

    Purpose

    Recombinant protein synthesis of human HSP90AB1

    Depositor

    Jeff Kuret

    Article

    Ayoub et al PLoS Genet. 2023 Mar 27;19(3):e1010681.

    Insert

    HSP90AB1 (HSP90AB1 Human)
    

    Use

    Tags

    6xHis-Thrombin

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. sfTq2ox-PBP5

    Plasmid

    #117960

    Purpose

    Periplasmic expression of superfolder mTurquoise2ox (pNM077 pTHV037-DsbAss-LEGPAGL-sfTq2ox-EFGS-∆1-17-PBP5)

    Depositor

    Tanneke den Blaauwen

    Article

    Meiresonne et al Mol Microbiol. 2019 Apr;111(4):1025-1038

    Insert

    sfTq2ox-PBP5 (dacA )
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pR-spondin 1

    Plasmid

    #128844

    Purpose

    bacterial expression of pR-spondin 1

    Depositor

    Marc de la Roche

    Article

    Urbischek et al Sci Rep. 2019 Apr 17;9(1):6193. doi: 10.

    Insert

    R-spondin 1 (RSPO1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. TAF3 PHD_pGEX-6P-2

    Plasmid

    #92100

    Purpose

    Expresses GST fusion TAF3 PHD domain protein

    Depositor

    Albert Jeltsch

    Article

    Kungulovski et al Epigenetics Chromatin. 2016 Mar 22;9:11.

    Insert

    TAF3 PHD domain (TAF3 Human)
    

    Use

    Tags

    GST Tag

    Expression

    Bacterial

    Mutation

    Promoter

    tac promoter

    Available since

    June 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pST-K

    Plasmid

    #44560

    Purpose

    for ectopic constitutive expression of mycobacterial proteins

    Depositor

    Vinay Nandicoori

    Article

    Parikh et al Appl Environ Microbiol. 2013 Mar;79(5):1

    Type

    Empty backbone

    Use

    E.coli-mycobacteria shuttle vector

    Tags

    Flag tag and His tag

    Expression

    Bacterial

    Mutation

    Promoter

    UV15 promoter

    Available since

    Oct. 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. L4440_BioBrick-SCR-sgRNA-A

    Plasmid

    #177811

    Purpose

    Overexpression of sgRNAs in E. coli HT115 (scrambled targeting sequences)

    Depositor

    Michael Ristow

    Article

    Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

    Insert

    Scrambled sgRNA targeting sequences
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge