30,260 results

1. pET21b(+)_SARS-CoV-2_3CLpro-Q306A

   Plasmid

   #177334

   Purpose

   bacterial expression of active SARS-CoV-2 3C-like proteinase

   Depositor

   Christopher Overall

   Article

   Pablos et al Cell Rep. 2021 Oct 26;37(4):109892. doi:

   Insert

   SARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2) (ORF1ab SARS-CoV-2 virus)
   

   Use

   Tags

   Factor Xa site-3xFlag-Myc-6xHis

   Expression

   Bacterial

   Mutation

   silent mutation C to T at position 10546 (elimina…

   Promoter

   T7

   Available since

   Nov. 23, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pSC27

   Plasmid

   #117478

   Purpose

   mobilizable, SRB-stable, kanamycin-resistant cloning vector

   Depositor

   Judy Wall

   Article

   Rousset et al Plasmid. 1998;39(2):114-22. doi: 10.1006

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJMK001

   Plasmid

   #33780

   Depositor

   Adam Cohen

   Article

   Kralj et al Science. 2011 Jul 15;333(6040):345-8.

   Insert

   Proteorhodopsin Optical Proton Sensor
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   D97N

   Promoter

   AraBAD

   Available since

   Dec. 14, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEX-4T-3-mR7BD

   Plasmid

   #79149

   Purpose

   bacterial expression of GST-mR7BD (Rab7 binding domain of murine RILP)

   Depositor

   Aimee Edinger

   Article

   Romero Rosales et al Mol Biol Cell. 2009 Jun;20(12):2831-40.

   Insert

   R7BD (Rilp Mouse)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   amino acids 220-299

   Promoter

   tac

   Available since

   June 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pES001 MBP-GSK3β-HA-His

   Plasmid

   #154010

   Purpose

   Expresses MBP-GSK3β-HA-His (human Glycogen Synthase Kinase 3β as a fusion protein with MBP, HA, and His- tags) in E.coli

   Depositor

   Jesse Zalatan

   Article

   Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

   Insert

   GSK3B (GSK3B Human)
   

   Use

   Tags

   HA tag, His6, and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   Tac

   Available since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKEN GFP mut2

   Plasmid

   #20409

   Depositor

   Raphael Valdivia, Stanley Falkow

   Article

   Cormack et al Gene. 1996 . 173(1 Spec No):33-8.

   Insert

   GFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   S65A V68L S72A

   Promoter

   Available since

   March 5, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28 His6-mEGFP-D4

   Plasmid

   #226372

   Purpose

   Production of recombinant mEGFP-D4, a fluorescent probe binding cholesterol

   Depositor

   Catherine Tomasetto

   Article

   Eichler et al Methods Mol Biol. 2025;2888:101-118. doi

   Insert

   D4 (pfoA Clostridium perfringens)
   

   Use

   Tags

   His6 and monomeric EGFP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET14-GFP30_Encap

   Plasmid

   #86406

   Purpose

   Expresses sfGFP tagged with a 30 amino acid CLP and encapsulin as an operon

   Depositor

   David Savage

   Article

   Cassidy-Amstutz et al Biochemistry. 2016 Jun 21;55(24):3461-8.

   Inserts

   sfGFP with a 30aa CLP tag
   Encapsulin
   

   Use

   Tags

   6x Histidine

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPath-Violacein-native

   Plasmid

   #200001

   Purpose

   Violacein pathway. Native promoter and CDS sequence

   Depositor

   Farren Isaacs

   Article

   Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

   Insert

   Pathways
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJSC111 - Bacterial expression plasmid for SpCas9-HF1 variant

    Plasmid

    #101209

    Purpose

    Bacterial expression plasmid for SpCas9-HF1 variant

    Depositor

    Jennifer Doudna, Keith Joung

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant N497A/R661A/Q695A/Q926A
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    N497A, R661A, Q695A and Q926A

    Promoter

    T7

    Available since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. FepA

    Plasmid

    #26039

    Depositor

    Johann Deisenhofer

    Article

    Buchanan et al Nat Struct Biol. 1999 Jan . 6(1):56-63.

    Insert

    FepA (fepA E Coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 3, 2010

    Availability

    Academic Institutions and Nonprofits only

12. pDSG358

    Plasmid

    #115599

    Purpose

    surface-displayed Ag1; medium copy

    Depositor

    Ingmar Riedel-Kruse

    Article

    Glass et al Cell. 2018 Jul 26;174(3):649-658.e16. do

    Insert

    regulated adhesin construct
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    See depositor comments below

    Promoter

    Available since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. poRibo-T2

    Plasmid

    #69347

    Purpose

    Ribo-T with orthogonal anti-Shine-Dalgarno sequence, and pL G to T mutation

    Depositor

    Michael Jewett

    Article

    Orelle et al Nature. 2015 Jul 29. doi: 10.1038/nature

    Insert

    oRibo-T
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHyo094 (eMutaT7)

    Plasmid

    #173124

    Purpose

    Expresses eMutaT7 for mutagenizing genes downstream from a T7 promoter in e.coli

    Depositor

    Seokhee Kim

    Article

    Park et al Nucleic Acids Res. 2021 Apr 6;49(6):e32.

    Insert

    pmCDA1-T7RNAP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. FLAG-Cdk9

    Plasmid

    #28100

    Depositor

    Andrew Rice

    Article

    Wang et al J Biol Chem. 2008 Nov 28. 283(48):33578-

    Insert

    Cdk9 (CDK9 Human)
    

    Use

    Tags

    FLAG

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    March 15, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. GCN5L2

    Plasmid

    #25482

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    GCN5L2 (KAT2A Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 23, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

17. pet21_TurboID_18A

    Plasmid

    #170922

    Purpose

    Express TurboID_18A in Bl21

    Depositor

    Huan Bao

    Article

    Bao et al Commun Biol. 2021 Apr 30;4(1):514. doi:

    Insert

    TurboID-18a
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28a-SnoopTagJr-MBP

    Plasmid

    #105628

    Purpose

    Plasmid for recombinant expression in bacteria of MBP fused to SnoopTagJr, permitting covalent linkage to DogTag by SnoopLigase

    Depositor

    Mark Howarth

    Article

    Buldun et al J Am Chem Soc. 2018 Feb 28;140(8):3008-3

    Insert

    SnoopTagJr-MBP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAK004

    Plasmid

    #233641

    Purpose

    For bacterial expression of eSpCas9(1.1) c-terminally tagged with VitelloTagA.

    Depositor

    Zak Swartz

    Article

    Clarke et al Development. 2024 Oct 15;151(20):dev2028

    Insert

    eSpCas9(1.1) with c-terminal VitelloTagA
    

    Use

    Tags

    6-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUBC-popTag-QtE-mScarlet

    Plasmid

    #240101

    Purpose

    Mammalian lentiviral vector for expressing the mScarlet-tagged 50nm-GEMs, encoded by the encapsulin protein from Quasibacillus thermotolerans and fused to a PopZ tag.

    Depositor

    Liam Holt

    Article

    Xie et al Mol Cell. 2024 Jul 25;84(14):2698-2716.e

    Insert

    Encapsulin
    

    Use

    Lentiviral

    Tags

    mScarlet and popZ

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    UBC promoter

    Available since

    June 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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