30,260 results

1. pHH0103_PRKACB_Pkinase

   Plasmid

   #110024

   Purpose

   Protein expression and purification of PRKACB_Pkinase

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

   Insert

   PRKACB_Pkinase (PRKACB Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pST50Trc2-HISNDHFR

   Plasmid

   #63958

   Purpose

   Position 2 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control

   Depositor

   Song Tan

   Article

   Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

   Type

   Empty backbone

   Use

   Tags

   6xHis; TEV cleavable

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. NAT1

   Plasmid

   #25511

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   NAT1 (NAT1 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 30, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

4. pSF4 TetCMV 5'TOP intron 20xGCN4 Renilla FKBP Stop 24xMS2v5 SV40 CTE polyA

   Plasmid

   #119946

   Purpose

   Expresses 5'TOP-SunTag-Renilla mRNA reporter in mammalian cells; note plasmid contains 24xGCN4 repeats

   Depositor

   Jeffrey Chao

   Article

   Wilbertz et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

   Insert

   Renilla luciferase
   

   Use

   Tags

   24xGCN4 repeats (SunTag), 24xMS2v5 stem loops in …

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   TetCMV

   Available since

   March 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJOE7706.1

   Plasmid

   #135075

   Purpose

   A shuttle vector for heterologous HA production with Corynebacterium glutamicum

   Depositor

   Josef Altenbuchner

   Article

   Hoffmann et al J Appl Microbiol. 2014 Sep;117(3):663-78

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pChuA

   Plasmid

   #42539

   Depositor

   Alan Jasanoff

   Article

   Lelyveld et al J Am Chem Soc. 2011 Feb 2;133(4):649-51.

   Insert

   ChuA (chuA E. coli O157:H7)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pColdI-DDX3_helicase_core

   Plasmid

   #233618

   Purpose

   Expression of His-tagged helicase core region of DDX3X in E.coli

   Depositor

   Shintaro Iwasaki

   Article

   Chen et al Cell Chem Biol. 2021 Apr 15;28(4):475-48

   Insert

   DDX3X helicase core WT (DDX3X Human)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pETDuet1-MBP-NAAEF-PNR(217-410 C275)-His6

   Plasmid

   #177849

   Purpose

   Bacterial Expression of PNR

   Depositor

   Karsten Melcher

   Article

   Tan et al PLoS One. 2013 Sep 12;8(9):e74359. doi:

   Insert

   photoreceptor cell-specific nuclear receptor (NR2E3 Human)
   

   Use

   Tags

   6xHis and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRExT2A-NT-PmNG

   Plasmid

   #232200

   Purpose

   Endogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for N-terminal tagging: PAC-3xTy-T2A-3xTy-mNeonGreen

   Depositor

   Calvin Tiengwe

   Article

   Carbajo et al Open Biol. 2025 Feb;15(2):240334. doi: 1

   Insert

   mNeongreen
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJJGL001

    Plasmid

    #180605

    Purpose

    Plasmid expressing E. coli codon optimized His-MBP-TEV-DpbCasX

    Depositor

    Jennifer Doudna

    Article

    Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6

    Insert

    DpbCasX
    

    Use

    CRISPR

    Tags

    10x His and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRS-QUE7mu

    Plasmid

    #129311

    Purpose

    Low concentration ATP measurement

    Depositor

    Hiroyuki Noji

    Article

    Yaginuma et al Sci Rep. 2014 Oct 6;4:6522. doi: 10.1038

    Insert

    QUEEN-7mu
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEX6P1-N-HA

    Plasmid

    #119756

    Purpose

    Allows cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained N-terminal HA tag.

    Depositor

    Martin Reijns, Andrew Jackson

    Type

    Empty backbone

    Use

    Tags

    GST and HA

    Expression

    Bacterial

    Mutation

    Promoter

    tac promoter

    Available since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSR83

    Plasmid

    #90211

    Purpose

    Expresses the response regulator OmpR, and expresses mCherry from the Cph8-OmpR regulated PompF-146 promoter

    Depositor

    Jeffrey Tabor

    Article

    Olson et al Mol Syst Biol. 2017 Apr 24;13(4):926. do

    Inserts

    OmpR (ompR E. coli)
    mCherry
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. dCas9-CRY2Clust

    Plasmid

    #200969

    Purpose

    induction of phase-separated condensate by CRY2Clust

    Depositor

    Jian Xu

    Article

    Kim et al Sci Adv. 2023 Mar 31;9(13):eadg1123. doi

    Insert

    CRY2Clust
    

    Use

    Lentiviral

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAP114

    Plasmid

    #120799

    Purpose

    E. coli-C. difficile shuttle vector for xylose-inducible expression in C. difficile; Pxyl driving expression of red fluorescent protein (mCherryOpt)

    Depositor

    Craig Ellermeier, David S. Weiss

    Article

    Muh et al J Bacteriol. 2019 Feb 11. pii: JB.00711-

    Insert

    Pxyl::mCherryOpt
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    1.4 kb xylR-Pxyl DNA fragment from C. difficile R…

    Promoter

    Pxyl

    Available since

    Feb. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET-Hisx6-rAPOBEC1-XTEN-nCas9-UGI-NLS

    Plasmid

    #89508

    Purpose

    Expression of Hisx6-rAPOBEC1-XTEN-nCas9-UGI-NLS in bacterial cells.

    Depositor

    Jin-Soo Kim

    Article

    Kim et al Nat Biotechnol. 2017 Feb 27. doi: 10.103

    Insert

    rAPOBEC1-nCas9 (D10A)-UGI-NLS
    

    Use

    Tags

    UGI and rAPOBEC-XTEN

    Expression

    Bacterial

    Mutation

    D10A mutation of Cas9

    Promoter

    T7

    Available since

    May 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKDsgRNA-rssA

    Plasmid

    #89957

    Purpose

    Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting rssA.

    Depositor

    Michael Lynch

    Article

    Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

    Insert

    rssA gRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pTet

    Available since

    June 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET11a-SUMO2

    Plasmid

    #53142

    Purpose

    E.coli expression of untagged mature human SUMO2 (amino acids 1-92)

    Depositor

    Frauke Melchior

    Article

    Flotho et al Methods Mol Biol. 2012;832:93-110. doi:

    Insert

    SUMO2 (SUMO3 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    mature form (amino acids 1-92)

    Promoter

    T7

    Available since

    June 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMBI

    Plasmid

    #17816

    Depositor

    Jay Keasling

    Article

    Martin et al Nat Biotechnol. 2003 Jul . 21(7):796-802

    Insert

    mevalonate kinase, phosphomevalonate kinase, mevalonate pyrophosphate decarboxylatse, IPP isomerase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 28, 2008

    Availability

    Academic Institutions and Nonprofits only

20. GST-mCh-FYVE

    Plasmid

    #192289

    Purpose

    Expresses GST- and mCherry-tagged HRS FYVE in e Coli

    Depositor

    James Hurley

    Article

    Jensen et al Sci Adv. 2022 Dec 14;8(50):eadd1436. doi

    Insert

    HRS FYVE domain
    

    Use

    Tags

    GST-TEV-mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Jan. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge