29,729 results

1. pHH0103_TEC-1/1

   Plasmid

   #91252

   Purpose

   Protein expression and purification of human SH3 domain construct TEC-1/1

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   TEC-1/1 (TEC Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pHH0103_ITK-1/1

   Plasmid

   #91253

   Purpose

   Protein expression and purification of human SH3 domain construct ITK-1/1

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   ITK-1/1 (ITK Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGLO-Blue

   Plasmid

   #220782

   Purpose

   Expresses a blue chromoprotein (visible light) with a his-tag and an inducible GFP (araBAD-promoter)

   Depositor

   Angela Leu

   Insert

   blue chromoprotein (aeBlue)
   

   Use

   Tags

   8xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pQE32-pr-mEosFP (mEosFP-V69T)

   Plasmid

   #99213

   Purpose

   Bacterial expression of the primed conversion capable protein pr-mEosFP (mEosFP-V69T).

   Depositor

   Periklis Pantazis

   Article

   Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

   Insert

   pr-mEosFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   monomeric variant with mutation V69T for primed c…

   Promoter

   T5

   Available since

   June 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a-KIRIN1

   Plasmid

   #107414

   Purpose

   Bacterial Expression plasmid for potassium ion raitometric indicator KIRIN1

   Depositor

   Robert Campbell

   Article

   Shen et al Commun Biol. 2019 Jan 14;2:18. doi: 10.1

   Insert

   KIRIN1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEX6P1-N-Strep2

   Plasmid

   #119758

   Purpose

   Allows cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained N-terminal Strep-II tag.

   Depositor

   Martin Reijns, Andrew Jackson

   Type

   Empty backbone

   Use

   Tags

   GST and Strep-tag II fusion tag

   Expression

   Bacterial

   Mutation

   Promoter

   tac promoter

   Available since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBx-Spas-sgRNA-Kan

   Plasmid

   #105233

   Purpose

   Plasmid containing S. pasteurianus sgRNA with no 20bp target (Empty control) Kanamycin marker for P. putida and P. fluorescens

   Depositor

   Kristala Prather

   Article

   Tan et al J Bacteriol. 2018 Mar 12;200(7). pii: JB

   Insert

   S. pasteurianus sgRNA
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLVX-RT005-HaloGPM

   Plasmid

   #163385

   Purpose

   2nd generation lentiviral vector to express sHalotag-sGFP module in the G-baToN system

   Depositor

   Monte Winslow

   Article

   Tang et al Elife. 2020 Oct 7;9. pii: 61080. doi: 10

   Insert

   Halotag-GFP-PDGFRtm-2A-mCherry (HaloGPM)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHT-ads

   Plasmid

   #47382

   Purpose

   Expresses ads under Pgrac promoter (inducible by IPTG) in bacteria

   Depositor

   Heng-Phon Too

   Article

   Zhou et al Biotechnol Bioeng. 2013 Mar 8. doi: 10.1

   Insert

   ads
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Pgrac

   Available since

   Oct. 24, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSHDY

    Plasmid

    #137661

    Purpose

    Conjugative vector with streamlined cloning capabilities. Contains two cloning sites for modular insertion of genetic parts.

    Depositor

    Ilka Axmann

    Article

    Behle et al ACS Synth Biol. 2020 Apr 17;9(4):843-855

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. 12.4kbVillin-ΔATG

    Plasmid

    #19358

    Depositor

    Deborah Gumucio

    Article

    Madison et al J Biol Chem. 2002 Sep 6. 277(36):33275-8

    Insert

    12.4kb Villin promoter
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    No ATG - transgenes cloned downstream of the vill…

    Promoter

    Available since

    Jan. 9, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28b-BE1

    Plasmid

    #73018

    Purpose

    Expresses BE1 with N-terminal His6 tag in E. Coli

    Depositor

    David Liu

    Article

    Komor et al Nature. 2016 Apr 20. doi: 10.1038/nature

    Insert

    BE1
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTetR-LasR-pLuxR-GFP

    Plasmid

    #171159

    Purpose

    Sensing Device with Reporter protein

    Depositor

    Matthew Chang

    Article

    Saeidi et al Mol Syst Biol. 2011 Aug 16;7:521. doi: 1

    Inserts

    lasR
    gfp
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pLux and pTet

    Available since

    July 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBTL-2

    Plasmid

    #22806

    Depositor

    Ryan Gill

    Article

    Lynch et al Biotechnol Bioeng. 2006 May 5. 94(1):151

    Insert

    
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 28, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAV116

    Plasmid

    #63183

    Purpose

    pRS416 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. mPapaya-pBAD

    Plasmid

    #54838

    Purpose

    Localization: Protein Expression Vector , Excitation: 530, Emission: 541

    Depositor

    Michael Davidson, Robert Campbell

    Article

    Hoi et al Chem Biol. 2013 Oct 24;20(10):1296-304.

    Type

    Empty backbone

    Use

    Tags

    6xHis and mPapaya

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Spacer Switch Plasmid

    Plasmid

    #215346

    Purpose

    Facilitates the change in spacer sequence to Neisseria gonorrhoeae encoding the Nla CRISPRi system.

    Depositor

    Hank Seifert

    Article

    Geslewitz et al mBio. 2023 Dec 21:e0302523. doi: 10.1128

    Insert

    OpaD spacer; Chloramphenicol Resistance Cassette
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET28a-TEV-TBP6.9F34MF37MF77M

    Plasmid

    #168268

    Purpose

    Expresses human TBP6.9 with the F34M/F37M/F77M triple mutant

    Depositor

    Joseph Wedekind

    Article

    Srivastava et al Crystals (Basel). 2021 Mar;11(3). doi: 1

    Insert

    TBP6.9
    

    Use

    Tags

    6xHis tag N-terminus, TEV protease cleavage site

    Expression

    Bacterial

    Mutation

    changed F34M, F37M and F77M

    Promoter

    T7 promotor

    Available since

    April 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLHCX-gpASNase1

    Plasmid

    #121526

    Purpose

    Expresses gpASNase1 in mammalian cells.

    Depositor

    Lucas Sullivan, Matthew Vander Heiden

    Article

    Sullivan et al Nat Cell Biol. 2018 Jul;20(7):782-788. d

    Insert

    gpASNase1
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDNA2.0 6H-FLAG-TEV-KRAS G12C

    Plasmid

    #111848

    Purpose

    Expresses activated human KRAS G12C (amino acids 1 - 169) in E. coli with amino terminal 6xHIS and FLAG tags that can be removed with TEV protease.

    Depositor

    Kenneth Westover

    Article

    Hunter et al Mol Cancer Res. 2015 Sep;13(9):1325-35.

    Insert

    KRAS G12C (KRAS Human)
    

    Use

    Tags

    6xHis-tag, FLAG-tag, and TEV protease cleavage se…

    Expression

    Bacterial

    Mutation

    Changed Glycine 12 to Cysteine

    Promoter

    T5

    Available since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge