30,744 results

1. pDEST14-DoubleCatcher

   Plasmid

   #216284

   Purpose

   Expresses SpyCatcher003-SpyCatcher003-TEV site-SpyTag003DA in bacterial cytoplasm, to allow stepwise coupling of two unique SpyTag-linked binders

   Depositor

   Mark Howarth

   Article

   Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

   Insert

   DoubleCatcher
   

   Tags

   His6 and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pQIq_MRGS_HIS8_(27F3)_FLAG

   Plasmid

   #199615

   Purpose

   Encodes DARPin-FLAG 27F3 for bacterial expression and column purification

   Depositor

   Shiva Tyagarajan

   Article

   Campbell et al Elife. 2022 Oct 31;11:e80895. doi: 10.75

   Insert

   DARPin-FLAG 27F3
   

   Tags

   8xHis and FLAG

   Expression

   Bacterial

   Promoter

   T5

   Available Since

   May 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. T4 Lysozyme WT*

   Plasmid

   #18111

   Depositor

   Brian Matthews

   Insert

   T4 Lysozyme (e Bacteriophage T4)
   

   Expression

   Bacterial

   Mutation

   No cysteines: C54T, C97A

   Available Since

   Aug. 26, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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4. pNCST-AvicFP4

   Plasmid

   #129507

   Purpose

   Expresses AvicFP4 constitutively in E. coli (most strains)

   Depositor

   Nathan Shaner

   Article

   Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

   Insert

   AvicFP4
   

   Expression

   Bacterial

   Mutation

   One of two variants of AvicFP4 tested with essent…

   Promoter

   synthetic constitutive (stationary phase) promote…

   Available Since

   Feb. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAKlux2.1

   Plasmid

   #14081

   Depositor

   Attila Karsi

   Article

   Karsi et al Plasmid. 2007 May;57(3):286-95. doi: 10.

   Insert

   Bacterial luciferase
   

   Expression

   Bacterial

   Available Since

   March 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEX-DAXX-ΔD/E-His

   Plasmid

   #179618

   Purpose

   Express GST-DAXX-ΔDE-His in bacteria

   Depositor

   Xiaolu Yang

   Article

   Huang et al Nature. 2021 Aug 18. pii: 10.1038/s41586

   Insert

   death domain associated protein (DAXX Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   deleted amino acids 424-495

   Available Since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBAD-KIRIN1-GR

   Plasmid

   #107417

   Purpose

   Bacterial Expression plasmid for potassium ion raitometric indicator KIRIN1-GR

   Depositor

   Robert Campbell

   Article

   Shen et al Commun Biol. 2019 Jan 14;2:18. doi: 10.1

   Insert

   KIRIN1-GR
   

   Expression

   Bacterial

   Available Since

   March 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pNCST-AvicFP2

   Plasmid

   #129505

   Purpose

   Expresses AvicFP2 constitutively in E. coli (most strains)

   Depositor

   Nathan Shaner

   Article

   Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

   Insert

   AvicFP2
   

   Expression

   Bacterial

   Promoter

   synthetic constitutive (stationary phase) promote…

   Available Since

   Feb. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEvol-NnSULT1C1-cysDNCQ

   Plasmid

   #188983

   Purpose

   biosynthesize sulfotyrosine in E. coli.

   Depositor

   Han Xiao

   Article

   Chen et al Nat Commun. 2022 Sep 16;13(1):5434. doi:

   Inserts

   NnSULT1C1
   cysD
   cysN
   cysC
   cysQ
   

   Expression

   Bacterial

   Available Since

   Sept. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBSV2_PresT-mCherryBb

    Plasmid

    #118238

    Purpose

    Kanamycin-resistant E.coli/B. burgdorferi shuttle vector; Encodes resT promoter driven mCherry

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

    Insert

    resT promoter driven mCherry
    

    Expression

    Bacterial

    Available Since

    Jan. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pOPINK-OTUD2 (full-length, aa 1-348)

    Plasmid

    #61409

    Purpose

    Expresses human OTUD2 (full-length) in E. coli.

    Depositor

    David Komander

    Article

    Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

    Insert

    OTUD2 (YOD1 Human)
    

    Tags

    His6-GST-3C

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBAD/myc-his A Rv0712 (FGE)

    Plasmid

    #16132

    Purpose

    Bacterial expression of Mycobacterium tuberculosis FGE

    Depositor

    Carolyn Bertozzi

    Article

    Carrico et al Nat Chem Biol. 2007 Jun . 3(6):321-2.

    Insert

    Formylglycine-generating enzyme
    

    Expression

    Bacterial

    Available Since

    Nov. 14, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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13. Mut1-RS

    Plasmid

    #182881

    Purpose

    A pEvol-based plasmid containing one inducible (by arabinose) copy of the Mut1-RS aaRS. The 2nd constitutive aaRS copy typically found in pEvol plasmids was deleted.

    Depositor

    Miriam Amiram

    Article

    Hadar et al Chembiochem. 2021 Apr 16;22(8):1379-1384

    Insert

    Mut1-RS
    

    Expression

    Bacterial

    Available Since

    July 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET-pro-siRNA-V2

    Plasmid

    #111086

    Purpose

    For production of recombinant siRNAs (pro-siRNAs) in E. coli.

    Depositor

    Linfeng HUANG

    Article

    Kaur et al Biotechnol Bioeng. 2018 Jun 5. doi: 10.1

    Type

    Empty backbone

    Use

    RNAi

    Expression

    Bacterial

    Available Since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1-CMV-mCherry-hPMCA2w/b

    Plasmid

    #111570

    Purpose

    Expresses a plasma membrane calcium pump hPMCA2w/b

    Depositor

    Baljit Khakh

    Article

    Yu et al Neuron. 2018 Aug 27. pii: S0896-6273(18)

    Insert

    Plasma membrane calcium pump 2, variant w/b (ATP2B2 Human)
    

    Tags

    mCherry

    Expression

    Bacterial

    Available Since

    Sept. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLys-M1 backbone

    Plasmid

    #109386

    Purpose

    Empty plasmid of the pLys-M1 construct. This is medium copy (p15A), CmR

    Depositor

    Tom Ellis

    Article

    Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    May 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. NylCp2-TS_pET28a

    Plasmid

    #215421

    Purpose

    Expression construct for NylCp2-TS gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    NylCp2-TS
    

    Expression

    Bacterial

    Mutation

    D122G/H130Y/D36A/E263Q

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET His6 ProteinG TEV LIC cloning vector (1P)

    Plasmid

    #29660

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Tags

    6xHis, Protein G, and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available Since

    June 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRSET YBX-1

    Plasmid

    #13035

    Depositor

    Peter Newburger

    Article

    Shen et al J Biol Chem. 1998 Mar 6. 273(10):5443-6.

    Insert

    YBX1 (YBX1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    Oct. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

20. pMM534

    Plasmid

    #112530

    Purpose

    pZE2-PLhrtO-luxCDABE - Tandem LuxCDABE expressed constitutively from promoter PLhrtO, ColE1 origin, KanR

    Depositor

    Timothy Lu

    Article

    Mimee et al Science. 2018 May 25;360(6391):915-918.

    Insert

    luxCDABE
    

    Expression

    Bacterial

    Promoter

    PL(HrtO)

    Available Since

    Feb. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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