31,059 results
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pOSIP-KC (KanR, PhiC31)
Plasmid#45982DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceJuly 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAB167
Plasmid#101672PurposeOpto-T7RNAP*(563), equal expr.DepositorInsertOpto-T7RNAP*(563), equal expr.
UseSynthetic BiologyExpressionBacterialAvailable SinceSept. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBFC1171
Plasmid#231994PurposeCRISPRi-ART crRNA only plasmid encoding crystal violet-inducible crRNA (for dRfxCas13d) with 2xBsaI spacer cloning siteDepositorInsertcrRNA of dRfxCas13d
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterpJEx (Jungle Express)Available SinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
PET-eSpCas9-NLS-6xHis
Plasmid#207379PurposeExpression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cellsDepositorInserteSpCas9
UseCRISPRTags6xHisExpressionBacterialMutationK848A, K1003A, R1060APromoterT7Available SinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSETA_crE6
Plasmid#172928PurposeCrowding FRET sensor with mCerulean3 and mCitrine for E. coli expression. Linker comprises 1 helix and 2 GSG linkersDepositorInsertCrowdingSensorE6
TagsHisExpressionBacterialPromoterT7Available SinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pExp-sfGFP
Plasmid#112573PurposeUsed for expression of protein in E. coli as TEV cleavable N-terminual 8His and sfGFP (superfolder green fluorescent protein) fusions with optional C-terminal StrepII-tagDepositorTypeEmpty backboneTags8xHis, Strep II, and sfGFPExpressionBacterialAvailable SinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAT-WT-HA-GFP11
Plasmid#177719PurposeER protein alpha-1-antitrypsin (WT) fused to an HA tag and the eleventh β-strand of GFPDepositorAvailable SinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_FAM98A-Halo
Plasmid#175329PurposeProduction of HaloTag fusion proteinDepositorAvailable SinceOct. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTHSSe_60
Plasmid#109254PurposeTALEsp2 stabilized promoter driving GFPDepositorInsertsfGFP
ExpressionBacterialPromoterTALEsp2 stabilized promoterAvailable SinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX-Cdc42
Plasmid#12201DepositorAvailable SinceAug. 11, 2006AvailabilityAcademic Institutions and Nonprofits only -
pPos-Ma-CmR-TAG111
Plasmid#197571PurposePositive selection plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses TAG-codon interrupted chloramphenicol resistance cassette and M. alvus Pyl-tRNA(6). p15a oriDepositorInsertsChloramphenicol resistance gene - 111TAG
T7 polymerase -TAG
GFPuv
M. alvus Pyl-tRNA(6)
ExpressionBacterialMutation111TAG and 8TAG/112TAGPromoterAraC, T7, cat promoter (constitutive), and lpp (c…Available SinceApril 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-28a(+)-GDH
Plasmid#108954Purposeheterologous expression of GDH of B. megaterium in E. coliDepositorInsertGlucose dehydrogenase IV (GDH IV)
TagsHis6-TagExpressionBacterialAvailable SinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSLC-254
Plasmid#124291PurposeExpression of vsfGFP-0. This construct improves brightness of sfGFP by fusing a GFP-specific single domain antibody to it that creates a dimeric fluorophore.DepositorInsertvsfGFP-0
ExpressionBacterialAvailable SinceSept. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pQmod3C-GG
Plasmid#191350PurposeClostridium expression vector (pCB102 origin, cmR) with drop-out Plac-RFP cassette flanked with BsaI sitesDepositorInsertPlac-RFP (IPTG-inducible red fluorescent protein)
ExpressionBacterialPromoterPlacAvailable SinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-PKM2-WT
Plasmid#122690PurposeExpress His-tagged PKM2 (wild type) protein in bacteria.DepositorAvailable SinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-J23111
Plasmid#113149PurposePlasmid constitutively expressing dCas9 proteinDepositorInsertdCas9
ExpressionBacterialMutationD10A & H840AAvailable SinceJan. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBHSTC104
Plasmid#124356PurposePlasmid expressing N- and C-terminal split fragments of gp41-1 intein inserted into CcdA antitoxinDepositorInsertgp41-1 N-intein and C-intein (bicistronic)
TagsCcdA-C (SFAD)ExpressionBacterialPromoterSTCAvailable SinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
LacI-pLacI-pIII pm
Plasmid#218100PurposePhagemid that expresses LacI, and pIII under the Lac promoterDepositorInsertpIII
ExpressionBacterialPromoterLac promoterAvailable SinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOSIP-CH (CamR, HK022)
Plasmid#45980DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceJuly 26, 2013AvailabilityAcademic Institutions and Nonprofits only
