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pQE60-EFTs-His

Plasmid

#124128

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Elongation factor Ts (tsf Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pTorPE-GEM-GECO1

Plasmid

#32463

Depositor

Robert Campbell

Article

Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

Insert

GEM-GECO1

Use

Lab constructed

Tags

6x His and modified TorA MNNNDLFQASRRRFLAQLG[G to…

Expression

Bacterial

Mutation

GCaMP3 L60P/K69E/N77Y/D86G/N98I/K119I/L173Q/T223S…

Available Since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only

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pWLxR5

Plasmid

#149490

Purpose

Broad-Host range expression vector, incW (pSa) origin, LuxR activator, PLuxB promoter, mRFP, Gentamicin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

LuxR-mRFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

PLuxB

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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LIC1B_Caspase-LOVN7C-7

Plasmid

#104629

Purpose

bacterial expression of light-activated caspase-3 with N7C-7 linker variation

Depositor

Jim Wells

Article

Smart et al Proc Natl Acad Sci U S A. 2017 Sep 26;11

Insert

Caspase-3 (CASP3 Human)

Tags

His and LOV2

Expression

Bacterial

Promoter

T7

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pCDF-NtXNtS1R1R2B2

Plasmid

#160882

Purpose

Expression of N. tabacum Rubisco small subunit (rbcS-S1), Rubisco chaperone protiens rbcX, raf1, bsd2 and raf2 in E. coli.

Depositor

Maureen Hanson

Article

Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

Insert

rbcS-S1, rbcX, raf1, raf2, bsd2

Expression

Bacterial

Promoter

rbcS-S1: T7, chaperones: T7

Available Since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-EGFP-anti-rabbit-IgG(Fc)-nanobody

Plasmid

#138133

Purpose

Expresses GST-tagged EGFP-anti-rabbit-IgG(Fc)-nanobody in E. coli cells.

Depositor

Kazuhisa Nakayama

Article

Katoh et al Mol Biol Cell. 2020 Jul 29:mbcE20040250.

Insert

EGFP-anti-rabbit-IgG(Fc)-nanobody

Tags

GST

Expression

Bacterial

Available Since

July 30, 2020

Availability

Academic Institutions and Nonprofits only

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pOSIP-TT (TetR, P21)

Plasmid

#45989

Depositor

Drew Endy, Keith Shearwin

Article

St-Pierre et al ACS Synth Biol. 2013 Sep 20;2(9):537-41.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 26, 2013

Availability

Academic Institutions and Nonprofits only

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pHH0103_YWHAQ_14-3-3

Plasmid

#109943

Purpose

Protein expression and purification of YWHAQ_14-3-3

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

YWHAQ_14-3-3

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pT7II-2N3Rtau

Plasmid

#249551

Purpose

Recombinant protein synthesis of 2N3R isoform of human Tau

Depositor

Jeff Kuret

Article

Pettis et al Anal Biochem. 2026 Mar 4;714:116095. doi

Insert

Tau (2N3R) (MAPT Human)

Expression

Bacterial

Available Since

Jan. 14, 2026

Availability

Academic Institutions and Nonprofits only

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pJ4M_hnRNPA2_FL_WT

Plasmid

#139109

Purpose

Express FL hnRNPA2 with a C-terminal MBP tag

Depositor

Nicolas Fawzi

Article

Ryan et al Nucleic Acids Res. 2020 Sep 1. pii: 5900

Insert

hnRNPA2 (HNRNPA2B1 Human)

Expression

Bacterial

Mutation

none

Available Since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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NSUN2

Plasmid

#188059

Purpose

Bacterial expression plasmid for human NSUN2; for activity testing

Depositor

Tanja Schirmeister

Article

Schwickert et al J Med Chem. 2022 Jul 18. doi: 10.1021/ac

Insert

NSUN2 (NSUN2 Human)

Tags

His-tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

Aug. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pET26-MBP-GLIC

Plasmid

#20887

Depositor

Raimund Dutzler

Article

Hilf et al Nature. 2009 Jan 1. 457(7225):115-8.

Insert

pentameric ligand gated ion channel from Gloeobacter violaceus PCC 7421

Tags

His, MBP, and pelB

Expression

Bacterial

Available Since

Oct. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pLG327

Plasmid

#128797

Purpose

Xylose inducible system without CRE site; cell do not need minimal media to express gfp under this modify xylose promoter.

Depositor

Christopher Voigt

Article

Gonzalez et al Nat Chem Biol. 2019 Dec 2. pii: 10.1038/

Insert

gfpmut2 (NEWENTRY Synthetic)

Expression

Bacterial

Promoter

Pxyl(s)

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pSANG10-3F

Plasmid

#39264

Depositor

John McCafferty

Article

Martin et al BMC Biotechnol. 2006 Dec 7;6:46.

Type

Empty backbone

Use

Bacterial expression, bacterial expression of re…

Tags

6xHis, pelB leader, and tri-FLAG

Expression

Bacterial

Promoter

T7

Available Since

Oct. 30, 2013

Availability

Academic Institutions and Nonprofits only

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pLG005 (Retron-TIR)

Plasmid

#157883

Purpose

Expresses the retron-TIR defense system

Depositor

Feng Zhang

Article

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

Retron-TIR

Expression

Bacterial

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pET-His-SCov2-nsp7

Plasmid

#154757

Purpose

Expression of SARS-CoV-2 nsp7

Depositor

Patrick Cramer

Article

Hillen et al Nature. 2020 May 21. pii: 10.1038/s41586

Insert

SARS-CoV-2 nsp7 (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Tags

6xHis-TEV

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX-2T PTP-1B

Plasmid

#8602

Depositor

Ben Neel

Insert

PTP-1B (PTPN1 Human)

Tags

GST

Expression

Bacterial

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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pJ2048.2

Plasmid

#140687

Purpose

3-color stripe (different sgRNAs)

Depositor

Yolanda Schaerli

Article

Santos-Moreno et al Nat Commun. 2020 Jun 2;11(1):2746. doi:

Insert

3-color stripe (different sgRNAs)

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 13, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX-Cdc42

Plasmid

#12201

Depositor

Jonathan Chernoff

Article

Bagrodia et al J Biol Chem. 1995 Sep 29. 270(39):22731-

Insert

Cdc42 (CDC42 Human)

Tags

GST

Expression

Bacterial

Available Since

Aug. 11, 2006

Availability

Academic Institutions and Nonprofits only

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pET28a-At-TRL

Plasmid

#32242

Depositor

Stan Fields, Jay Hesselberth

Article

Schutz et al RNA. 2010 Mar;16(3):621-31. Epub 2010 Ja

Insert

A. thaliana tRNA ligase (RNL Mustard Weed)

Tags

6xHistidine

Expression

Bacterial

Promoter

T7 RNA Polmyerase

Available Since

July 5, 2013

Availability

Academic Institutions and Nonprofits only

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