31,624 results
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Plasmid#177310PurposetRNA synthetase/tRNA pair for the in vivo incorporation of 7-Fluoro-L-Tryptophan (7FTrp) into proteins in E. coli in response to the amber (TAG) codonDepositorInserttRNA synthetase
ExpressionBacterialPromoterT7Available SinceJan. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-45b-HOXD13-IDR-mCherry
Plasmid#145280PurposeBacterial Expression of 6xHis HOXD13 IDR mCherry Fusion ProteinDepositorInsertHOXD13-IDR (HOXD13 Human)
ExpressionBacterialAvailable SinceDec. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX-GST-AGO2
Plasmid#24317DepositorAvailable SinceMay 11, 2011AvailabilityAcademic Institutions and Nonprofits only -
COC053
Plasmid#209131PurposeEnzymatic biotinylation of proteins at a specific lysine residue in a recognition sequence. This has multiple applications, especially in the study of protein-protein interactions.DepositorInsertEnzyme BirA (birA E. coli)
ExpressionBacterialAvailable SinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSW036
Plasmid#140034PurposeKO of PCC 11901 acsA gene/expresses YFP under control of the cpt promoterDepositorInsertYellow fluorescent protein
ExpressionBacterialPromotercptAvailable SinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHS0537 CRISPR-associated IscB locus (Delaware Bay) in pBR322 with Fn spacer
Plasmid#176588PurposeEndogenous locus of CRISPR-associated IscB (Delaware Bay) with Fn spacerDepositorInsertCRISPR-associate IscB (Delaware Bay) locus
ExpressionBacterialMutationReplaced spacer in CRISPR array with Fn spacerAvailable SinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGS140_6xHis-EGFP-I3-01
Plasmid#211889PurposeBacterial expression of I3-01DepositorInsertI3-01
ExpressionBacterialMutationN/AAvailable SinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLG004 (RT-Abi-P2)
Plasmid#157882PurposeExpresses the RT-Abi-P2 defense systemDepositorInsertRT-Abi-P2
ExpressionBacterialAvailable SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGSKU
Plasmid#72243PurposeContains the CORE cassette with convergent KlURA3 and KanMX4 markers and the I-SceI gene under the inducible GAL1 promoterDepositorInsertsKlURA3
kanMX4
I-SceI
ExpressionBacterialAvailable SinceJan. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNR220
Plasmid#79541PurposeInput plasmid: expresses three serine recombinases (TP901, BxbI, and A118) downstream of three inducible promoters.DepositorInsertsTP901
BxbI
A118
TagsHis tag and LAA degradation tagExpressionBacterialAvailable SinceJuly 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTn7xTS_SpyCatcher003:mNG
Plasmid#225190PurposeUsed for inserting SpyCatcher003 fused to mNeonGreen at the bacterial Tn7 siteDepositorInsertSpyCatcher003:mNeonGreen
TagsSpyCatcherExpressionBacterialAvailable SinceApril 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAX1_HS_gpN:SpyTag003
Plasmid#225194PurposeUsed for adding C-terminal SpyTag003 to the E. coli HS P2 phage major capsid proteinDepositorInsertgpN homology arms and SpyTag003
ExpressionBacterialAvailable SinceApril 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGex-6p2_NSD2
Plasmid#221376PurposeExpresses NSD2 in bacterial cellsDepositorAvailable SinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQFT-mNG-Pcon-Scar
Plasmid#203678PurposepQFT-mNG with constitutively expressed mScarlet-IDepositorInsertsmNeonGreen
mScarlet-I
ExpressionBacterialPromoterPQJ and Phyb18Available SinceDec. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQFT-mNGsacB
Plasmid#203676PurposepQFT with mNeonGreen and sacB under control of PQJDepositorInsertsmNeonGreen
sacB
ExpressionBacterialMutationHas synonymous (silent) mutations to remove BsrGI…PromoterPQJAvailable SinceDec. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET30-R5-GFP
Plasmid#17838DepositorInsertpET30-GFP
UseSynthetic BiologyTagsHis, R5, and S-tagExpressionBacterialAvailable SinceMay 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pDsRed-monomer-hAIM2
Plasmid#51544PurposeExpress DsRed monomer-tagged hAIM2 in bacteriaDepositorAvailable SinceMarch 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
MBP-C5E-6XTUBE
Plasmid#217628PurposeBacterial expression of MBP tagged 6XTUBEDepositorInsert6XTUBE
ExpressionBacterialAvailable SinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDual-eGFP(H66)
Plasmid#63558PurposeExpression of the Azure (blue) spectral variant of eGFP in bacteria and in mammalian cells. Could be used as a reporter for quantifying gene targeting and recombineering in mammalian cells.DepositorInsertHis-T7-eGFP(H66)
UseLentiviralTagsHis6 and T7ExpressionBacterial and MammalianMutationChanged tyrosine at position 66 with respect to t…PromoterCMV-EF1α hybrid (CEF)Available SinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJOE6227.1
Plasmid#135078PurposeAn integration vector for the chromosomal deletion of the upp gene in P. putida KT2440; carries the upp deletion cassette.DepositorInsertup- and downstream regions of upp (upp Pseudomonas putida)
ExpressionBacterialAvailable SinceFeb. 5, 2020AvailabilityAcademic Institutions and Nonprofits only