30,749 results

1. pBADHisB-Sumo-mBaoJin

   Plasmid

   #229580

   Purpose

   Expresses mBaoJin in bacterial cells

   Depositor

   Kiryl Piatkevich

   Article

   Zhang et al Nat Methods. 2024 Apr;21(4):657-665. doi

   Insert

   mBaoJin
   

   Expression

   Bacterial

   Available Since

   June 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPM2213

   Plasmid

   #167553

   Purpose

   Encodes rfb region of Shigella flexneri serotype 2a, which expresses Shigella O antigen in E. coli K-12

   Depositor

   Renato Morona

   Article

   Macpherson et al Mol Microbiol. 1991 Jun;5(6):1491-9. doi

   Insert

   rfb region of Shigella flexneri serotype 2a
   

   Expression

   Bacterial

   Available Since

   May 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET41-MBP-t-A3A-t-His

   Plasmid

   #109231

   Purpose

   Bacterial expression of A3A with N-terminal MBP an C-terminal His tag, both cleavable with TEV protease

   Depositor

   Rahul Kohli

   Article

   Schutsky et al Nat Biotechnol. 2018 Oct 8. pii: nbt.420

   Insert

   human APOBEC3A (APOBEC3A Optimized for E. coli expression, Human)
   

   Tags

   His and MBP

   Expression

   Bacterial

   Available Since

   Oct. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pWUR703

   Plasmid

   #53082

   Purpose

   inducible TtAgoDM (D478A,D546A) expression

   Depositor

   John van der Oost

   Article

   Swarts et al Nature. 2014 Mar 13;507(7491):258-61. do

   Insert

   TtAgo
   

   Tags

   Strep-II tag

   Expression

   Bacterial

   Mutation

   D478A D546A

   Promoter

   T7

   Available Since

   May 13, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pETM43 p62 FL (1-440)

   Plasmid

   #210425

   Purpose

   Bacterial expression of MBP-p62 FL (1-440) with 3C cleavage site

   Depositor

   Carsten Sachse

   Article

   Tarafder et al Methods Mol Biol. 2019;1880:3-15. doi: 1

   Insert

   p62 FL (NUP62 Human)
   

   Tags

   6xHis and MBP

   Expression

   Bacterial

   Available Since

   Feb. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTriEx-PA-Rac1

   Plasmid

   #22024

   Purpose

   Expression of photoactivatable Rac1

   Depositor

   Klaus Hahn

   Article

   Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

   Insert

   PA-Rac1 (RAC1 Human, Avena sativa (oat))
   

   Tags

   6X His

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Rac1 starts at I4 and contains mutations Q61L, E9…

   Promoter

   CMV, p10

   Available Since

   Sept. 29, 2009

   Availability

   Academic Institutions and Nonprofits only

7. peSCBE3-NG-Hypa

   Plasmid

   #218165

   Purpose

   This plasmid harbors the base editor eSCBE3-NG-Hypa along with an sgRNA cloning cassette, facilitating high-efficiency and high-fidelity cytosine base editing at targets bearing NG PAM in Streptomyces

   Depositor

   Yuhui Sun

   Article

   Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:

   Inserts

   escbe3-NG-Hypa
   a programmable sgRNA cloning cassette
   

   Use

   CRISPR; Sgrna transcription

   Expression

   Bacterial

   Mutation

   Mutation in the portion of deaminase hAPOBEC3A : …

   Promoter

   rpsL promoter

   Available Since

   May 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBSV2G_PflaB-msfGFPBb

   Plasmid

   #118231

   Purpose

   Gentamicin-resistant E.coli/B. burgdorferi shuttle vector; Encodes flagellin promoter driven msfGFP

   Depositor

   Christine Jacobs-Wagner

   Article

   Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

   Insert

   flagellin promoter driven msfGFP
   

   Expression

   Bacterial

   Available Since

   Nov. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pWZ853

   Plasmid

   #208115

   Purpose

   mSwAP-In marker cassette 2 with 5’PB-mNeongreen-HPRT1-BSD

   Depositor

   Jef Boeke

   Article

   Zhang et al Nature. 2023 Nov 1. doi: 10.1038/s41586-

   Insert

   mNeonGreen-HPRT1-BSD
   

   Tags

   T2A

   Expression

   Bacterial and Mammalian

   Promoter

   EF1alpha

   Available Since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. TccCas13a Twin SUMO

    Plasmid

    #199754

    Purpose

    Expresses codon optimized thermostabile TccCas13a in E.coli

    Depositor

    Magdy Mahfouz

    Article

    Mahas et al Proc Natl Acad Sci U S A. 2022 Jul 12;11

    Insert

    TccCas13a
    

    Tags

    SUMO

    Expression

    Bacterial

    Promoter

    LacI

    Available Since

    June 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRECMYC-Ebu1

    Plasmid

    #240713

    Purpose

    pLAM12 vector backbone containing Ebu1 recombitron with MspRecT and a donor in the ncRNA to target MSMEG_5894 gene of Mycobacterium smegmatis mc2 155

    Depositor

    Seth Shipman

    Article

    González-Delgado et al bioRxiv 2025.06.16.660010

    Insert

    Ebu1 RT, Eco1 ncRNA, MspRecT
    

    Expression

    Bacterial

    Mutation

    WT

    Available Since

    Aug. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJAK184.tetR.PT5-3.dCas9

    Plasmid

    #232482

    Purpose

    optimized ahTet inducible dCas9 for insertion into C. difficile genome

    Depositor

    James Collins

    Article

    Chua et al Microbiol Resour Announc. 2025 Apr 28:e0

    Insert

    Catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    TetR-PT5-3

    Available Since

    April 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJC.15A.sgRNA.TA

    Plasmid

    #232483

    Purpose

    sgRNA cloning backbone for expression of sgRNA in C. difficile. Contains TA system to enable maintenence without antibiotic selection.

    Depositor

    James Collins

    Article

    Chua et al Microbiol Resour Announc. 2025 Apr 28:e0

    Inserts

    Toxin-antitoxin System from CD630
    medium-copy-number p15A origin of replication
    mCherry
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    P3 constitutive promoter and native promoter

    Available Since

    April 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCW-LIC-N8C_Fab2b

    Plasmid

    #203692

    Purpose

    Expresses N8C_Fab2b

    Depositor

    Brenda Schulman

    Article

    Henneberg et al Nat Chem Biol. 2023 Dec;19(12):1513-1523

    Insert

    N8C_Fab2b
    

    Expression

    Bacterial

    Available Since

    Sept. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pE5770

    Plasmid

    #170777

    Purpose

    expression of hybrid proteins in E. coli

    Depositor

    Anthony Schryvers

    Article

    Chaudhuri et al Methods Mol Biol. 2022;2414:115-140. doi

    Insert

    Kan resistance (kanR )
    

    Tags

    polyhisbioMbpTEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. p2B-56

    Plasmid

    #177925

    Purpose

    Expresses optimized unleaky reverse tetracycline transactivator (rtTA-M2-SE-G72P) under control of yeast MYO2 promoter

    Depositor

    Vadim Gladyshev

    Article

    Barre et al (unpublished)

    Insert

    Optimized unleaky reverse tetracycline transactivator (rtTA-M2-SE-G72P) under control of yeast MYO2 promoter
    

    Expression

    Bacterial and Yeast

    Mutation

    V9I; F67S; G72P, F86Y; R171K

    Promoter

    MYO2

    Available Since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pS24·Pm-GFP

    Plasmid

    #122594

    Purpose

    Expresses GFP under the regulatory control of Pm. Expression needs additional xyls gene

    Depositor

    Pablo Ivan Nikel

    Article

    Volke et al Microb Biotechnol. 2019 Mar 12. doi: 10.

    Insert

    Pm promoter
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    Pm

    Available Since

    March 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTA-Mob 2.0 Tp

    Plasmid

    #188619

    Purpose

    Mutated pTA-Mob 2.0 Backbone in TraJ Promoter Region

    Depositor

    Bogumil Karas

    Article

    Cochrane et al Biodes Res. 2022 Aug 19;2022:9802168. do

    Type

    Empty backbone

    Use

    Synthetic Biology; Conjugation helper plasmid

    Expression

    Bacterial and Yeast

    Available Since

    May 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAG87 FAST

    Plasmid

    #130719

    Purpose

    Expresses His-tagged FAST (also called YFAST) in bacteria

    Depositor

    Arnaud Gautier

    Article

    Plamont et al Proc Natl Acad Sci U S A. 2016 Jan 19;11

    Insert

    FAST
    

    Tags

    His-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEND-164_Cacnes_pFAST

    Plasmid

    #225616

    Purpose

    Replicative vector for anaerobic fluorescent reporter protein FAST tag strong constitutive recombinant expression in C. acnes. pBRESP36A with P(BBa_J23119)+RBS_1+pFAST.

    Depositor

    Marc Güell

    Article

    Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

    Insert

    pFAST
    

    Use

    Synthetic Biology

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Codon optimized for Cutibacterium acnes

    Available Since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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