29,740 results

1. His_CL7_2NLS_iCas12a-4NLS

   Plasmid

   #222971

   Purpose

   Bacterial expression of NLS-rich iCas12a construct

   Depositor

   Jennifer Doudna

   Article

   Chen et al Nat Commun. 2024 Feb 26;15(1):1727. doi:

   Insert

   His_CL7_2NLS_iCas12a-4NLS
   

   Use

   CRISPR

   Tags

   CL7 and His

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   July 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pet16b-Avi-ubiquitin_rbs_BirA

   Plasmid

   #134897

   Purpose

   Expresses HIS-Avi-tagged ubiquitin for recombinant protein production

   Depositor

   Andrew Deans

   Article

   Tan et al PLoS One. 2020 Feb 24;15(2):e0229000. do

   Inserts

   Ubiquitin (UBB Human)
   BirA
   

   Use

   Tags

   10HIS-AviTag-PreScission(3C)protease

   Expression

   Bacterial

   Mutation

   Promoter

   RBS and T7

   Available since

   April 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pOPINB-OTULIN (full-length, aa 1-352)

   Plasmid

   #61464

   Purpose

   Expresses human OTULIN (full-length) in E. coli.

   Depositor

   David Komander

   Article

   Keusekotten et al Cell. 2013 Jun 6;153(6):1312-26. doi: 10

   Insert

   OTULIN (OTULIN Human)
   

   Use

   Tags

   His6-3C

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET26b-Granzyme A

   Plasmid

   #8823

   Depositor

   Judy Lieberman

   Article

   Beresford et al Proc Natl Acad Sci U S A 1997 Aug 19;94(

   Insert

   Human Granzyme A (GZMA Human)
   

   Use

   Tags

   (His)6 and Enterokinase site

   Expression

   Bacterial

   Mutation

   it mature enzyme.

   Promoter

   Available since

   Sept. 26, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEB1030

   Plasmid

   #22067

   Depositor

   Emmanuelle Bouveret

   Article

   Battesti et al Proteomics. 2008 Nov . 8(22):4768-71.

   Type

   Empty backbone

   Use

   Tags

   T18-Flag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 22, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28C-mCherry-53BP1LCD

   Plasmid

   #204407

   Purpose

   E. coli expression of the 53BP1 low-complexity domain (1208-1977) with an N-terminal mCherry tag

   Depositor

   Samie Jaffrey

   Article

   Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

   Insert

   53BP1(1208-1977) (TP53BP1 Human)
   

   Use

   Tags

   6x-His; mCherry

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. dCas9-CRY2Clust-BRD4ΔN

   Plasmid

   #200968

   Purpose

   induction of phase-separated condensate mediated by BRD4dN-IDR

   Depositor

   Jian Xu

   Article

   Kim et al Sci Adv. 2023 Mar 31;9(13):eadg1123. doi

   Insert

   BRD4dN
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Aug. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. p3035 GST-HPV-18 E6

   Plasmid

   #10850

   Depositor

   Peter Howley

   Article

   Huibregtse et al EMBO J. 1991 Dec . 10(13):4129-35.

   Insert

   HPV18 E6 (E6 Human papillomavirus)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 13, 2006

   Availability

   Academic Institutions and Nonprofits only

9. IVT-nCas9(H840A)

   Plasmid

   #207455

   Purpose

   in vitro transcription of mRNA

   Depositor

   Erik Sontheimer

   Article

   Liu et al Nat Biotechnol. 2023 Sep 14. doi: 10.103

   Insert

   H840A
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. luxB:Venus+luxA/pRSETB Duet

    Plasmid

    #197582

    Purpose

    BRET lux luciferase with Venus for bacterial expression

    Depositor

    Takeharu Nagai

    Article

    Kaku et al Sci Rep. 2021 Jul 22;11(1):14994. doi: 1

    Insert

    LuxA LuxBVenus
    

    Use

    E.coli expression

    Tags

    His X 6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGEX-4T1 human PP2A B56α full length

    Plasmid

    #132635

    Purpose

    expresses human PP2A B56α full length in E. coli

    Depositor

    Wenqing Xu

    Article

    Sangodkar et al J Clin Invest. 2017 Jun 1;127(6):2081-20

    Insert

    PP2A B56α
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAP05

    Plasmid

    #102920

    Purpose

    Direct cloning and broad-host-range heterologous expression vector

    Depositor

    Bradley Moore

    Article

    Zhang et al MBio. 2017 Sep 5;8(5). pii: e01291-17. d

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    May 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. CAMK2A

    Plasmid

    #38971

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    CAMK2A (CAMK2A Human)
    

    Use

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

14. pFCM1

    Plasmid

    #64948

    Purpose

    source of CmR FRT cassette

    Depositor

    Herbert Schweizer

    Article

    Choi et al Nat Methods. 2005 Jun;2(6):443-8.

    Insert

    FRT-cat-FRT
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMJ841

    Plasmid

    #39318

    Depositor

    Jennifer Doudna

    Article

    Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    Aspartate 10 to Alanine (D10A) and Histidine 840 …

    Promoter

    T7

    Available since

    Aug. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. pOPINJ

    Plasmid

    #26045

    Depositor

    Ray Owens

    Article

    Berrow et al Nucleic Acids Res. 2007 . 35(6):e45.

    Insert

    HisGST3C-lacZ
    

    Use

    Tags

    MAHHHHHH_GST_LEVLFQGT

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    Available since

    Oct. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET20b- hTPI

    Plasmid

    #50723

    Purpose

    overexpression of human TPI1 in E.coli

    Depositor

    Markus Ralser

    Article

    Gruning et al Open Biol. 2014 Mar 5;4(3):130232. doi:

    Insert

    Triosephosphate isomerase 1 (TPI1 Human)
    

    Use

    Tags

    6xHIS tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 30, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. HPF1-c004

    Plasmid

    #216874

    Purpose

    Bacterial expression vector for E. coli producing full length HPF1 protein with N-terminal YFP fusion

    Depositor

    Lari Lehtiö

    Article

    Dhakar et al Sci Rep. 2024 Feb 16;14(1):3875. doi: 10

    Insert

    Full length HPF1 (HPF1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMRE-Tn7-152

    Plasmid

    #118566

    Purpose

    miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    sGFP2
    

    Use

    Minitn7 delivery vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCDF-Mm2

    Plasmid

    #197100

    Purpose

    To express the variant of Methanosarcina mazei PylRS specific for pyrrolysine derivatives (Boc-Lys, Az-ZLys, AzAmZLys) and its cognate Pyl tRNA and allow UAG codon to be translated into these derivati

    Depositor

    Kensaku Sakamoto

    Article

    Yamaguchi et al Bioconjug Chem. 2016 Jan 20;27(1):198-20

    Insert

    PylRS variant, Pyl tRNA, kanamycin resistance gene
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Lys61, Glu131, Ala306, Phe384, Glu444 (PylRS)

    Promoter

    Available since

    June 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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