31,059 results

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pETDuet-1 Hepatitis C Virus NS3/NS4A

Plasmid

#61696

Purpose

inducible dual expression of NS3 protease along with its cofactor NS4A

Depositor

Michael Johnson

Article

Lee et al PLoS One. 2013 Oct 9;8(10):e75144. doi:

Inserts

NS4A
NS3

Tags

his

Expression

Bacterial

Promoter

T7

Available Since

April 10, 2015

Availability

Academic Institutions and Nonprofits only

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pST5552

Plasmid

#36255

Purpose

Mycobacteria replicating vector expressing eGFP.

Depositor

Carolyn Bertozzi, Jessica Seeliger

Article

Seeliger et al PLoS One. 2012;7(1):e29266. Epub 2012 Ja

Insert

eGFP

Use

Synthetic Biology; Mycobacteria replicating vector

Expression

Bacterial

Promoter

ModifiedHsp60

Available Since

May 15, 2012

Availability

Academic Institutions and Nonprofits only

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pKSJ376

Plasmid

#103065

Purpose

Expresses colicin E1 with R domain deletion and ImmE1

Depositor

Alan Finkelstein, Karen Jakes

Article

Jakes et al J Bacteriol. 2016 Dec 13;199(1). pii: e0

Inserts

colicin E1 immunity protein (pColE1_26, imm E. coli)
cea/colicin E1

Expression

Bacterial

Mutation

R (receptor-binding domain) deletion in colicin E…

Promoter

T7 lac (from pET) and T7 lac (from pET52-b)

Available Since

Dec. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn5-161

Plasmid

#118541

Purpose

miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mTurquoise2

Use

Minitn5 delivery vector

Expression

Bacterial

Available Since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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PE-SUMO CRYBB1

Plasmid

#217675

Purpose

For expression of SUMO tagged human beta B1 crystallin

Depositor

Larry David

Insert

CRYBB1 (CRYBB1 Human)

Tags

6XHis and Smt3

Expression

Bacterial

Promoter

T7

Available Since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

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hsDCX-EGFP-pHAT

Plasmid

#83918

Purpose

human Doublecortin, N-terminal 6-His tag, C-terminal EGFP-Strep-tag II

Depositor

Gary Brouhard

Article

Bechstedt et al Dev Cell. 2012 Jul 17;23(1):181-92. doi:

Insert

Doublecortin isoform b (DCX Human, NM_178152.2)

Tags

6-His and EGFP+Strep-tag II

Expression

Bacterial

Available Since

Dec. 8, 2016

Availability

Academic Institutions and Nonprofits only

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mu-IFNg-pCIneo

Plasmid

#163517

Purpose

Expresses murine IFN gamma in mammalian cells

Depositor

Thomas Weber

Insert

murine-IFN gamma (Ifng Mouse)

Expression

Bacterial and Mammalian

Available Since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pCDF RBP-FL P53

Plasmid

#174042

Purpose

Express Full length P53 in E.coli with cleavable expression/purification tag (ribose binding protein)

Depositor

Stewart Loh

Article

Blanden et al Elife. 2020 Dec 2;9. pii: 61487. doi: 10

Insert

P53 gene (TP53 Human)

Tags

Thermoanaerobacter Tencongenesis ribose binding p…

Expression

Bacterial

Promoter

T7

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pSK+KanaRpsL

Plasmid

#20871

Depositor

Jiyue Zhu

Article

Wang et al Mol Biotechnol. 2009 May;42(1):110-6. do

Insert

KanamycinResistance-RpsL

Expression

Bacterial

Mutation

none

Available Since

May 5, 2009

Availability

Academic Institutions and Nonprofits only

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pTOX2

Plasmid

#127447

Purpose

Encodes MqsR toxin. CAM-R

Depositor

Matthew Waldor

Article

Lazarus et al Appl Environ Microbiol. 2019 Jun 14. pii

Insert

mqsR toxin

Expression

Bacterial

Promoter

pRha

Available Since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

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pMycVec2

Plasmid

#32361

Depositor

Michael Niederweis

Article

Kaps et al Gene. 2001 Oct 31;278(1-2):115-24.

Insert

pJAZ38 ori

Expression

Bacterial

Available Since

Nov. 21, 2011

Availability

Academic Institutions and Nonprofits only

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pOPINS-USP21 (USP, aa 196-565)

Plasmid

#61585

Purpose

Expresses human USP21 (USP domain) in E. coli.

Depositor

David Komander

Article

Ye et al EMBO Rep. 2011 Apr;12(4):350-7. doi: 10.

Insert

USP21 (Ubiquitin carboxyl-terminal hydrolase 21) (USP21 Human)

Tags

His6-SUMO

Expression

Bacterial

Mutation

Deleted aa 1-195.

Promoter

T7

Available Since

March 18, 2015

Availability

Academic Institutions and Nonprofits only

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pGEX2T E7

Plasmid

#13634

Depositor

Karl Munger

Article

Wu et al J Virol. 1993 Apr . 67(4):2402-7.

Insert

HPV 16 E7 (E7 Human)

Tags

GST

Expression

Bacterial

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
pBS mShh (CT#258)

Plasmid

#13999

Depositor

Cliff Tabin

Insert

Shh (Shh Mouse)

Expression

Bacterial

Available Since

Feb. 9, 2007

Availability

Academic Institutions and Nonprofits only

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pET3-ARF6-His6(CO)

Plasmid

#201887

Purpose

Used for bacterial expression of human protein ARF6

Depositor

Richard Kahn

Insert

ARF6 (ARF6 Human)

Tags

6xHis (GSGSGSHHHHHH)

Expression

Bacterial

Mutation

Codon optimized for bacterial expression

Available Since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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6xHis-MBP-TEV-mDvl2PDZ(264-353)_pCDFduet

Plasmid

#216386

Purpose

Bacterial expression vector for MBP-tagged mouse Dvl2 PDZ domain (residues 264-353)

Depositor

William Weis

Article

Mahoney et al Sci Signal. 2022 Aug 23;15(748):eabo2820

Insert

Dishevelled-2 (Dvl2 Mouse)

Tags

6xHis-MBP-TEV

Expression

Bacterial

Promoter

T7

Available Since

April 12, 2024

Availability

Academic Institutions and Nonprofits only

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pH6HTC_IGF2BP2a-Halo

Plasmid

#175309

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

IGF2BP2 (IGF2BP2 Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available Since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pJKR-O-mphR

Plasmid

#62570

Purpose

Macrolide biosensor. Medium copy plasmid that expresses GFP proportional to macrolide concentration. Compatible with all other pJKR plasmids.

Depositor

George Church

Article

Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

Insert

mCherry

Expression

Bacterial

Available Since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-CAT

Plasmid

#173943

Purpose

Bacterial expression of isolated PARP1 CAT domain

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-CAT (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pAW019-2

Plasmid

#85614

Purpose

Inducible dCas9 integration vector for Bacillus subtilis

Depositor

Perry Chou

Article

Westbrook et al Appl Environ Microbiol. 2016 Jun 3. pii:

Insert

dcas9

Expression

Bacterial

Promoter

PxylA (B. megaterium)

Available Since

Jan. 6, 2017

Availability

Academic Institutions and Nonprofits only

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