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31,059 results

Showing: 3901 - 3920 of 31059 results

  1. pBDK

    Plasmid
    #135190
    Purpose
    Integration and excision of kanamycin cassette for the E. coli genome via CreI, SceI, and lamba Red system. Contains SacB selection
    Depositor
    Hongqing Fang
    Article
    Wei et al PLoS One. 2017 Oct 24;12(10):e0186891. d
    Insert
    KanR
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    cat
    Available Since
    March 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    135190-plasmid-map-sequence-id-273774

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  5. pAcHELIX-sgRNA_entry (CJT94)

    Plasmid
    #181783
    Purpose
    Expresses AcHELIX containing a nicking I-AniI fusion to AcTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).
    Depositor
    Benjamin Kleinstiver
    Article
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    nAniI-AcTnsB, AcTnsC, AcTniQ, AcCas12k
    Expression
    Bacterial
    Mutation
    nAniI = K227M, F80K, L232K
    Promoter
    Lac and J23119
    Available Since
    March 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    181783-plasmid-map-sequence-id-358223

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  10. pGal4x5-E4T-G210

    Plasmid
    #171077
    Purpose
    The resulting plasmid was used to generate a Gal4x5-E4-Gless 210bp construct for ampilifying the immobilized template for assays. Originally came from Michael Carey, was submitted with his permission
    Depositor
    Joan Conaway
    Article
    He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11
    Insert
    Gal4-E4-Gless-cassette
    Expression
    Bacterial
    Promoter
    T7 promoter
    Available Since
    July 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    171077-plasmid-map-sequence-id-335554

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  12. pTSara

    Plasmid
    #60720
    Purpose
    Expresses both fragments of T7 RNAP split at position 179. Both fragments are driven by the arabinose inducible promoter PBAD. Also contains a constitutive araC ORF
    Depositor
    Matthew Bennett
    Article
    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11
    Inserts
    N Terminal fragment of T7 RNAP split betwen 179-180
    N Terminal fragment of T7 RNAP split betwen 179-180
    Expression
    Bacterial
    Mutation
    T7 RNAP split between positions 179-180 and wt T7…
    Promoter
    PBAD
    Available Since
    Dec. 18, 2014
    Availability
    Academic Institutions and Nonprofits only
    60720-plasmid-map-sequence-id-97706

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  13. pAP114

    Plasmid
    #120799
    Purpose
    E. coli-C. difficile shuttle vector for xylose-inducible expression in C. difficile; Pxyl driving expression of red fluorescent protein (mCherryOpt)
    Depositor
    Craig Ellermeier, David S. Weiss
    Article
    Muh et al J Bacteriol. 2019 Feb 11. pii: JB.00711-
    Insert
    Pxyl::mCherryOpt
    Expression
    Bacterial
    Mutation
    1.4 kb xylR-Pxyl DNA fragment from C. difficile R…
    Promoter
    Pxyl
    Available Since
    Feb. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    120799-plasmid-map-sequence-id-235314

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  15. pSNK

    Plasmid
    #135193
    Purpose
    Arabinose inducible expression of lamba-Red recombinase proteins and I-SceI. Contains kamamycin resistance cassette
    Depositor
    Hongqing Fang
    Article
    Wei et al PLoS One. 2017 Oct 24;12(10):e0186891. d
    Insert
    I-SceI lamba Red
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    None
    Promoter
    araBAD
    Available Since
    April 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    135193-plasmid-map-sequence-id-288121

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  17. SENP2M497A

    Plasmid
    #61088
    Purpose
    cleave the SUMO off when use PETSUMO vector for express fusion protein
    Depositor
    Cynthia Wolberger
    Article
    Wiener et al Nat Struct Mol Biol. 2013 Sep;20(9):1033
    Insert
    SENP2M497A catalytic domain (SENP2 Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    M497A; Catalytic domain is residues 364-589
    Promoter
    T7
    Available Since
    Jan. 29, 2015
    Availability
    Academic Institutions and Nonprofits only

  19. GST-thr-RAF1-RBD

    Plasmid
    #86033
    Purpose
    Expresses the RAS-binding domain of RAF1 for in vitro analysis of RAS activation
    Depositor
    Dominic Esposito
    Insert
    RAF1 RBD (Ras-binding domain) (RAF1 Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Contains residues 1-149
    Promoter
    T7
    Available Since
    Jan. 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    86033-plasmid-map-sequence-id-170056

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  20. pTriEx-mCherry-PA-Rac1-T17N

    Plasmid
    #22029
    Purpose
    Expression of photoactivatable Rac1 (dominant-negative mutant).
    Depositor
    Klaus Hahn
    Article
    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.
    Insert
    PA-Rac1-T17N (RAC1 Avena sativa (oat), Human)
    Tags
    6xHis and mCherry
    Expression
    Bacterial, Insect, and Mamm…
    Mutation
    LOV(Leu404-Leu546). Rac1 starts at Ile4 and conta…
    Promoter
    CMV, p10
    Available Since
    Sept. 29, 2009
    Availability
    Academic Institutions and Nonprofits only
    22029-plasmid-map-sequence-id-239317

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Showing: 3901 - 3920 of 31059 results