34,213 results
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Plasmid#182881PurposeA pEvol-based plasmid containing one inducible (by arabinose) copy of the Mut1-RS aaRS. The 2nd constitutive aaRS copy typically found in pEvol plasmids was deleted.DepositorInsertMut1-RS
ExpressionBacterialAvailable sinceJuly 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
AzoRS-4
Plasmid#182884PurposeA pEvol-based plasmid containing one inducible (by arabinose) copy of the AzoRS-4 aaRS. The 2nd constitutive aaRS copy typically found in pEvol plasmids was deleted.DepositorInsertAzoRS-4
ExpressionBacterialAvailable sinceJuly 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYTK-DN1
Plasmid#180282PurposeCas9 cassette (TU1)DepositorInsertConLS - pPGK1 - Cas9 - tPGK1 - ConR1
ExpressionBacterialPromoterpPGK1Available sinceJune 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
L4440_BioBrick-hsf-1-sgRNA-B
Plasmid#177787PurposeOverexpression of sgRNAs in E. coli HT115 (targeting C. elegans hsf-1 promoter)DepositorInserthsf-1 (hsf-1 Nematode)
ExpressionBacterialAvailable sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDT-SuperFi-Cas9
Plasmid#183630PurposeExpression and purification of N-terminal His-MBP tagged SuperFi Cas9 in E. coliDepositorInsertSuperFi-Cas9
UseCRISPRTags6xHis-MBPExpressionBacterialMutationY1010D, Y1013D, Y1016D, V1018D, R1019D, Q1027D, …PromoterT7Available sinceMay 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGJJ045
Plasmid#183621PurposeabundancePCA plasmid - Contains a barcode landing pad and two fragments of the murine methotrexate-resistant DHFR, the expression of one driven by the CYC promoter and the other by the GPD promoter.DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable sinceMay 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBHM2406
Plasmid#173442PurposepRS316-mNeonGreen-CBP-2XFLAG-HYGB, contains C. neoformans optimized mNeonGreen and HYG marker for use as PCR templateDepositorInsertmNeonGreen-CBP-2XFLAG-HYGB
TagsCBP-2xFLAGExpressionBacterial and YeastAvailable sinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHN1002
Plasmid#178831PurposeBacterial expression for human Saposin ADepositorInsertartificial gene encoding human Saposin A (AR Human)
TagsHis8, 3CExpressionBacterialMutationCodon-usage was optimized for bacterial expressio…PromoterT7Available sinceJan. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSF-G1TFAKRS
Plasmid#177311PurposetRNA synthetase/tRNA pair for the in vivo incorporation of N6-(trifluoroacetyl)-L-lysine (TFA-Lys) into proteins in E. coli in response to the amber (TAG) codonDepositorInserttRNA synthetase
ExpressionBacterialPromoterT7Available sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_IGF2BP2a-Halo
Plasmid#175309PurposeProduction of HaloTag fusion proteinDepositorPublicationAvailable sinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSB219
Plasmid#164125Purposep15A backbone with PhlF binding sitesDepositorInsertsfGFP
ExpressionBacterialPromoterJ23105Available sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPSU30
Plasmid#173830PurposeExpresses 30 kD Penn State ladder proteinDepositorInsertHSTPABPACS100BCBP
ExpressionBacterialPromoterT7Available sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPSU10
Plasmid#173827PurposeExpresses 10 kD Penn State ladder proteinDepositorInsertSTRHSTPAB
ExpressionBacterialPromoterT7Available sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPSU60
Plasmid#173833PurposeExpresses 60 kD Penn State ladder proteinDepositorInsertHSTPABPACRCC1
ExpressionBacterialPromoterT7Available sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX-GST-His6-1_HBD
Plasmid#171869PurposeExpresses a specific DNA-RNA hybrid sensor protein with the HBD domain of RNase H1 in bacterial cellsDepositorInsertHybrid binding domain of Rnase H1
TagsGST- and His10-tagExpressionBacterialMutationWTAvailable sinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTU1-A-SP44-mScarlet-I
Plasmid#163756PurposeExpresses Streptomyces codon-optimised mScarlet-IDepositorInsertmScarlet-I
UseSynthetic BiologyTagsFlag and FlAsH tagsExpressionBacterialPromoterSP44Available sinceJuly 30, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAT218
Plasmid#134886PurposeBacterial expressionDepositorInserteGFP
UseSynthetic BiologyExpressionBacterialAvailable sinceJuly 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAT203
Plasmid#134880PurposeBacterial expressionDepositorInsertmRFP1
UseSynthetic BiologyExpressionBacterialAvailable sinceJuly 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28-AviTag-DogCatcher-MBP
Plasmid#171928PurposeExpresses AviTag-DogCatcher-MBP in bacterial cytoplasm. The AviTag enables site-specific biotinylation by BirA.DepositorInsertAviTag-DogCatcher-MBP
TagsHis6 and Thrombin cleavage siteExpressionBacterialMutationDogCatcher added to N-terminus of MBP via a GSGES…PromoterT7Available sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTetR-LasR-pLuxR-GFP
Plasmid#171159PurposeSensing Device with Reporter proteinDepositorInsertslasR
gfp
UseSynthetic BiologyExpressionBacterialPromoterpLux and pTetAvailable sinceJuly 15, 2021AvailabilityAcademic Institutions and Nonprofits only