32,127 results
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Plasmid#34623DepositorExpressionBacterialMutationHis67 to Arg , Glu216 to Asn, Asp217 to Gly, Phe2…PromoterPtac and Ptac (from SepRS- operon like)Available SinceFeb. 2, 2012AvailabilityAcademic Institutions and Nonprofits only
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GST-p35
Plasmid#24895DepositorAvailable SinceFeb. 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
pTcI38-Wasabi
Plasmid#86117PurposemWasabi reporter gene gated by temperature-sensitive Lambda repressor TcI38DepositorInsertsTcI38
mWasabi
Tags6xHisExpressionBacterialMutationA67T, M1V, L65S, K68R, F115L, D126G, D188GPromoterpR/pL and pR/pL, LacIAvailable SinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pST-KT
Plasmid#44561Purposefor ectopic inducible expression of mycobacterial proteinsDepositorTypeEmpty backboneUseE.coli-mycobacteria shuttle vectorTagsFlag tag and His tagExpressionBacterialPromoterUV15 promoterAvailable SinceOct. 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR-RFP entry
Plasmid#192278PurposeEntry vector for cloning MmCas12m spacers. RFP flanked by MmCas12m CRISPR repeats can be digested out with BbsI.DepositorInsertRFP flanked by type V-M CRISPR repeat sequences
UseCRISPRExpressionBacterialPromoterPJ23119Available SinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSetB-KanR-LiLac
Plasmid#184569PurposeFluorescent reporter for lactate (with lifetime and intensity response); for bacterial or in vitro expressionDepositorInsertLiLac fluorescent sensor of lactate
TagsHis7ExpressionBacterialPromoterT7Available SinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28b-His tagged nuclear Pif1
Plasmid#65047Purposeexpression of the nuclear form of Pif1p (Pif1p-delta40), Nterminal His tagDepositorInsertYeast Pif1, nuclear form, N-terminal 6x His-tag (PIF1 Budding Yeast)
TagshistagExpressionBacterialPromoterT7 promoterAvailable SinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBSJL1
Plasmid#71261PurposeE. coli-Veillonella shuttle plasmidDepositorTypeEmpty backboneUseE. coli-veillonella shuttle plasmidExpressionBacterialAvailable SinceJan. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBAF
Plasmid#104152PurposePlasmid for expression of BAF (BANF1) in E.coli.Coding sequence in pET15B.DepositorInsertBarrier-to-autointegration factor (BAF) (BANF1 Human)
TagsN-terminal His tag with thrombin cleavage siteExpressionBacterialAvailable SinceJan. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-DEST-His
Plasmid#119763PurposeAllows Gateway cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained C-terminal 10xHis tag.DepositorTypeEmpty backboneTags10xHis and GSTExpressionBacterialPromotertac promoterAvailable SinceJan. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET42b-ABE7.10
Plasmid#120398PurposeExpresses ABE7.10-NLS with an N-terminal His Tag (His6) for bacterial expressionDepositorInsertABE7.10
TagsHisExpressionBacterialMutationMammalian codon-optimizedPromoterT7Available SinceApril 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pmPAGFP_IRES_puro2
Plasmid#21046DepositorInsertpmPAGFP
ExpressionBacterial and MammalianAvailable SinceMay 15, 2009AvailabilityAcademic Institutions and Nonprofits only -
pBbA5k-acrAB-sfGFP
Plasmid#195516PurposeInducible expression vector for transcriptionally fused acrAB and sfGFP.DepositorInsertacrAB
UseSynthetic BiologyTagssfgfpExpressionBacterialPromoterlacUV5Available SinceFeb. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHCL151
Plasmid#134455PurposeTetR, lacI-Plac-ssdsbA-mscarN, Integrative plasmid for the expression of fully-screted prey. N-terminal mScarlet-I fusion.DepositorInsertlinker-mScarlet-I for C-terminal fusion
ExpressionBacterialPromoterplacAvailable SinceJan. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSW003-PpsbA-mNeonGreen
Plasmid#205019PurposeBroad host-range bacterial expression vector with constitutive PsbA promoter; mNeonGreen (codon optimized for P. fluorescens)DepositorInsertmNeonGreen
ExpressionBacterialMutationmNeonGreen is codon optimized for expression in P…PromoterPpsbAAvailable SinceSept. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDule-3-nitroTyrosine (5B)
Plasmid#85498PurposePlasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the Mj 3NY (5B) synthetase and cognate amber suppressing tRNA in Ecoli.DepositorInsert3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
TagsNoneExpressionBacterialMutationY32H H70C D158S I159A L162RPromoterlpp (constitutive)Available SinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNR220
Plasmid#79541PurposeInput plasmid: expresses three serine recombinases (TP901, BxbI, and A118) downstream of three inducible promoters.DepositorInsertsTP901
BxbI
A118
TagsHis tag and LAA degradation tagExpressionBacterialAvailable SinceJuly 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Spike
Plasmid#173921PurposeExpresses the original SARS-Cov-2 Spike Protein under a CMV PromoterDepositorInsertSARS-2 Spike Protein (S Synthetic, Human, SARS-COV-2)
Tagsno tags, native proteinExpressionBacterial and MammalianPromoterstandard CMVAvailable SinceApril 11, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAR357_SFP_pCDF-1b
Plasmid#122851PurposeCo-expresses the phosphopantheteine transferase Sfp of Bacillus subtilis to transfer a PPant arm to acyl carrier proteins in Escherichia coli. Untagged; in pCDF-1b vector.DepositorInsertSfp
ExpressionBacterialPromoterT7 promoterAvailable SinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
Pet22b-mEGFP
Plasmid#166439PurposeBacterial expression of 6x His tagged mEGFPDepositorInsertmEGFP
ExpressionBacterialAvailable SinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only