31,059 results
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Plasmid#25256PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertNRASA:M1-N172 (NRAS Human)
TagsHisExpressionBacterialMutationContains amino acids M1-N172Available SinceAug. 13, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGR596
Plasmid#213787PurposeIntegrates at the CAN1 locus the BadBoy3 TP-DNAP1 variant and the Nourseothricin resistance marker. Includes an I-SceI transient expression cassetteDepositorInsertBadBoy2 TP-DNAP1
UseSynthetic BiologyExpressionBacterial and YeastMutationSee manuscriptAvailable SinceMarch 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUCP30T-mKo1
Plasmid#78477PurposeBroad range bacterial fluorescence labelingDepositorInsertmKO1
ExpressionBacterialAvailable SinceAug. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pOSIP-TH (TetR, HK022)
Plasmid#45988DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceJuly 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMJ825
Plasmid#39315DepositorInsertCas9
UseCRISPRTagsHis6 and MBPExpressionBacterialMutationAspartate 10 to Alanine (D10A)PromoterT7Available SinceAug. 28, 2012AvailabilityAcademic Institutions and Nonprofits only -
MazF-WT
Plasmid#155202PurposeTo express recombinant MazF protein.DepositorInsertMazF
ExpressionBacterialAvailable SinceSept. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-TS2126 RnlA-Strep
Plasmid#159350PurposeExpress TS2126 RNA ligase with His6 tag and Strep tag at N and C terminal, respectivelyDepositorInsertTS2126 RNA ligase
TagsHis6 and StrepExpressionBacterialPromoterT7Available SinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
EcRho
Plasmid#113121PurposeExpresses E. coli RhoDepositorInsertRho
TagsMGHExpressionBacterialPromoterT7Available SinceOct. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
UEV1
Plasmid#25619PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceAug. 31, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET21-pelB-LaMIgG-14
Plasmid#228994PurposeFor bacterial expression of anti-mouse IgG nanobody LaMIgG-14, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.DepositorInsertLaMIgG-14
Tags6xHISExpressionBacterialPromoterT7Available SinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaTdT-39
Plasmid#228980PurposeFor bacterial expression of anti-tdTomato nanobody LaTdT-39, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.DepositorInsertLaTdT-39
Tags6xHISExpressionBacterialPromoterT7Available SinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET11a-CA(A14C, E45C)
Plasmid#79045PurposeBacterial vector for cloning and propagation in DH5alphaDepositorInsertCA (A14C, E45C)
ExpressionBacterialMutationA14C and E45C mutations in HIV-1 CA proteinPromoterT7Available SinceMarch 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLG027 (qatABCD)
Plasmid#157905PurposeExpresses the qatABCD defense systemDepositorInsertqatABCD (ATPase + unknown protein + QueC homolog + TatD DNase)
ExpressionBacterialAvailable SinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLibacceptorV2
Plasmid#106250PurposeRecombination-mediated cassette exchange with target genomic lociDepositorTypeEmpty backboneUseCre/Lox and Synthetic BiologyExpressionBacterialAvailable SinceNov. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBx-Spas-sgRNA-Gm
Plasmid#105232PurposePlasmid containing S. pasteurianus sgRNA with no 20bp target (Empty control) Gentamycin marker for P. aeruginosaDepositorInsertS. pasteurianus sgRNA
UseCRISPR and Synthetic BiologyExpressionBacterialAvailable SinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pReg-RamR
Plasmid#190610PurposeExpresses the RamR transcription factor in E. coliDepositorInsertRamR
ExpressionBacterialAvailable SinceJan. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
luxA+luxB/pRSETB Duet
Plasmid#197581PurposeExpressing luxA and luxB under dual T7 promoterDepositorInsertLuxA LuxB
TagsT7 tag and 6xHisExpressionBacterialPromoterT7Available SinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKAR2-Br512g3.1
Plasmid#171200PurposeExpresses Br512g3.1 polymerase in E.coliDepositorInsertEngineered Bst DNA Polymerase I Large Fragment
Tags8x His tag; Villin Headpiece 47 amino acid (vHP47…ExpressionBacterialPromoterT7Available SinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDiGc
Plasmid#59322PurposeInducible expression of dsRed and constitutive expression of GFP in bacteriaDepositorInsertsEGFP
dsRed
ExpressionBacterialPromoterPbad and rpsMAvailable SinceOct. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET-29b(+)_NSP4ss_SerM_NucA-myc
Plasmid#218191PurposeBacterial expression of a periplasmically expressed myc-tagged Serratia Marcescens NucA (Benzonase)DepositorArticleInsertnucA
TagsNSP4 signal secretion peptide and mycExpressionBacterialMutationcodon optimized ecoli residues - 22-266PromoterT7Available SinceMay 17, 2024AvailabilityAcademic Institutions and Nonprofits only