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Bacterial Expression

30,753 results

Showing: 4061 - 4080 of 30753 results

  1. pT7-pelB

    Plasmid
    #157740
    Purpose
    empty vector; N-terminal PelB signal sequence; potential C-terminal His6; T7 promoter; like pET-20b, but additionally avoids added residues in final protein since cloning uses BseRI restriction sites
    Depositor
    Debra Hansen
    Type
    Empty backbone
    Tags
    PelB signal sequence and possible His6-tag (adds …
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    June 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    157740-plasmid-map-sequence-id-334002

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  2. pGEX4T1-Opt WWP1

    Plasmid
    #179327
    Purpose
    E. coli optimized expression of GST-fusion of human WWP1
    Depositor
    Arthur Haas
    Insert
    WWP1 (WWP1 Human)
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Codon optimized for expression in E. coli
    Available Since
    April 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    179327-plasmid-map-sequence-id-358706

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  6. His 3C MKK3 1-347

    Plasmid
    #87878
    Purpose
    E. coli expression of MKK3 aa 1-347
    Depositor
    Peter Mace
    Insert
    MKK3 (MAP2K3 Human)
    Tags
    3C protease site and 6xHis
    Expression
    Bacterial
    Mutation
    aa 1-347
    Promoter
    T7
    Available Since
    March 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    87878-plasmid-map-sequence-id-174923

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  7. pET28-SBP-TEV

    Plasmid
    #36943
    Purpose
    SGC Empty backbone for bacterial expression
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Tags
    6xHistag-SBP-TEV
    Expression
    Bacterial
    Available Since
    June 19, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits
    36943-plasmid-map-sequence-id-46249

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  8. p2B-55

    Plasmid
    #177924
    Purpose
    Expresses optimized unleaky reverse tetracycline transactivator (rtTA-M2-SE-G72P) under control of yeast TDH3 promoter
    Depositor
    Vadim Gladyshev
    Article
    Barre et al (unpublished)
    Insert
    Optimized unleaky reverse tetracycline transactivator (rtTA-M2-SE-G72P) under control of yeast TDH3 promoter
    Expression
    Bacterial and Yeast
    Mutation
    V9I; F67S; G72P, F86Y; R171K
    Promoter
    TDH3
    Available Since
    Dec. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    177924-plasmid-map-sequence-id-349994

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  9. pMO9075

    Plasmid
    #117481
    Purpose
    empty complementation vector for Desulfovibrio: contains promoter from Aph(3')-IIa but no RBS, contains pBG1 and pMB1 replicons, SpecR
    Depositor
    Judy Wall
    Article
    Keller et al Methods Enzymol. 2011;497:503-17. doi: 1
    Insert
    Aph(3')-IIa promoter
    Expression
    Bacterial
    Promoter
    Aph(3')-IIa promoter
    Available Since
    Feb. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    117481-plasmid-map-sequence-id-273023

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  12. pET-28b-T7-henAsCas12a-HF1(E174R/N282A/S542R/K548R)-NLS(nucleoplasmin)-6xHis (AAS1935)

    Plasmid
    #114073
    Purpose
    T7 promoter bacterial expression plasmid for human codon optimized enAsCas12a-HF1(E174R/N282A/S542R/K548R) with C-terminal NLS and His-tag
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized enAsCas12a-HF1 (E174R/N282A/S542R/K548R) with C-terminal NLS and His-tag
    Tags
    NLS(nucleoplasmin) and 6xHis
    Expression
    Bacterial
    Mutation
    E174R, S542R, K548R and N282A
    Available Since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114073-plasmid-map-sequence-id-220529

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  14. pET28-SpRY-HF1-NLS-6xHis (gbr2102)

    Plasmid
    #181744
    Purpose
    bacterial expression plasmid for SpRY-HF1 with an n-terminal MKIEE MBP tag, and c-terminal bi-partite NLS and 6x-His tag
    Depositor
    Benjamin Kleinstiver
    Article
    Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038
    Insert
    bacterial codon optimized SpRY-HF1
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS(SV40)-6xHis and MKIEE MBP tag
    Expression
    Bacterial
    Mutation
    SpRY-HF1, SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E…
    Promoter
    T7
    Available Since
    Sept. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    181744-plasmid-map-sequence-id-373171

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  15. pD451-pA-APEX2

    Plasmid
    #157863
    Purpose
    Bacterial expression plasmid to express pA-APEX2 for antibody-based biotin proximity labeling
    Depositor
    Alex Kentsis
    Insert
    pA-APEX2
    Expression
    Bacterial
    Available Since
    Aug. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    157863-plasmid-map-sequence-id-308764

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  19. PANK3

    Plasmid
    #32872
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PANK3 (PANK3 Human)
    Tags
    His tag (with Thrombin cleavage)
    Expression
    Bacterial
    Mutation
    amino acids 10-370 only
    Available Since
    Jan. 6, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 4061 - 4080 of 30753 results