31,059 results
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Plasmid#36915DepositorInsertCre recombinase
ExpressionBacterialAvailable SinceJuly 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pK27Sumo_His-SUMO-nsp14-nsp10 fusion (SARS-CoV-2)
Plasmid#169160PurposeTo express nsp14-nsp10 fusion protein in E. coliDepositorInsert14His-SUMO-nsp14-GGSGGS-nsp10 (ORF1ab Synthetic, SARS-CoV-2)
Tags14His-SUMO and Fusion protein of SARS-CoV-2 nsp14…ExpressionBacterialMutationCodon optimised for E. coliPromoterT5Available SinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-B4E
Plasmid#162067PurposeFor expresion of the B4E fusion protein, consists of the ampR gene (encoding the enzyme β-lactamase without the native signal sequence) fused to residues 28-215 of the murine eIF4EDepositorInsertampR-eIF4E fusion protein
Tags6xHis tagExpressionBacterialMutationeIF4E incorporating the K119A mutation, which is …PromoterT7Available SinceFeb. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pST39-Ubc13-MMS2
Plasmid#184807PurposeBacterial expression of yeast UEV-E2 complexDepositorInsertUbc13, MMS2
TagsHis6ExpressionBacterialPromoterT7Available SinceJune 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
FEV_pet28a
Plasmid#131668PurposeBacterial expression of ETS gene FEVDepositorAvailable SinceOct. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCJH019_SC101_pLAC_Cas12c(4)_Kan
Plasmid#183071PurposeWild-type Cas12c_4 in a plasmid containing a SC101 origin and lac promoter for in vivo interference assay.DepositorInsertCas12c_4
ExpressionBacterialMutationWild-typeAvailable SinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSA110
Plasmid#59784Purposedrives mCherry in C. elegans intestinal cellsDepositorInsertsExpressionBacterialAvailable SinceNov. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET-45b-HP1a-mCherry
Plasmid#185012PurposeBacterial Expression of 6xHis CBX5 (HP1a) mCherry Fusion ProteinDepositorInsertCBX5 (HP1a) (Cbx5 Mouse)
ExpressionBacterialAvailable SinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pXD69m1-TetW
Plasmid#191246PurposeE. coli - Eggerthella lenta shuttle plasmid with tetracycline resistance in E. lentaDepositorTypeEmpty backboneExpressionBacterialAvailable SinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBP-C_ccdB
Plasmid#204114PurposeType 0 CDS partDepositorInsertToxic DNA gyrase inhibitor ccdB
ExpressionBacterialMutationNoneAvailable SinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCARSF63
Plasmid#64695PurposeH6-TRX-Smt3-GB1-NpuDnaEN102-CBDDepositorTypeEmpty backboneTagsCBD, HisTag, NpuDnaE-N102, Smt3, and TRXExpressionBacterialPromoterT7Available SinceSept. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-hygMV
Plasmid#73891Purposefission yeast drug selection markerDepositorInserthygMV
ExpressionBacterial and YeastPromoterpombe TEFAvailable SinceNov. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBAT5
Plasmid#112581PurposeUsed for expression of protein in E. coli as non-fusions without initiation ATGDepositorTypeEmpty backboneExpressionBacterialAvailable SinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-3-SpSuc1
Plasmid#162558PurposeExpresses GST-tagged S.pombe Suc1 in E.coliDepositorInsertSchizosaccharomyces pombe Suc1 (suc1 Fission Yeast)
ExpressionBacterialAvailable SinceSept. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJ211_WT_low
Plasmid#128848PurposeLow copy number plasmid for expression of E. coli flagellin from its natural promoterDepositorInsertFlagellin
ExpressionBacterialAvailable SinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCCM
Plasmid#162708PurposeSecond CCM operon cloned from H. neapolitanus; pFA expressing the remaining 11 genes in the H. neapolitanus CCM clusterDepositorInsertSecond CCM operon cloned from H. neapolitanus
ExpressionBacterialPromoterPLtet0-1 promoterAvailable SinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDB038a
Plasmid#99211PurposeAccessory plasmid for PACE of PylRS variantsDepositorInsertPpsp [SD8] gIII(P29*,Y184*), luxAB; PproK pylT
ExpressionBacterialMutationamber codons at positions 29 and 184 of gIIIPromoterPpsp and PproKAvailable SinceOct. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDB007(+)
Plasmid#99147PurposeAccessory plasmid for PACE of MjTyrRS variantsDepositorInsertPT7 [SD8] gIII, luxAB; PproK tyrT(Opt, CUA)
ExpressionBacterialPromoterPT7 and PproKAvailable SinceNov. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1 UBXN2A
Plasmid#113505PurposeBacterial expression of GST-tagged UBXN2ADepositorAvailable SinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBSV2G_PflaB-msfYFPBb
Plasmid#118233PurposeGentamicin-resistant E.coli/B. burgdorferi shuttle vector; Encodes flagellin promoter driven msfYFPDepositorInsertflagellin promoter driven msfYFP
ExpressionBacterialAvailable SinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
HC25 pComb3xss anti-Homer1 nanobody
Plasmid#134718PurposeBacterial expression of anti-Homer1 (Mouse) recombinant llama nanobody for use as an immunolabelDepositorInsertanti-Homer1 (Mouse) recombinant llama nanobody HC25 (Homer1 L. glama (llama))
Tags6xHis-HAExpressionBacterialPromoterlacAvailable SinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGV3341
Plasmid#170279PurposeExpression of mutant HIV-2 RT p66 reverse transcriptaseDepositorInsertHIV-2 RT p66 (mutant)
ExpressionBacterialPromoterT7-lacAvailable SinceJune 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSETB PKA Cat
Plasmid#14920DepositorInsertPKA catalytic subunit alpha (Prkaca Mouse)
ExpressionBacterialAvailable SinceJuly 24, 2007AvailabilityAcademic Institutions and Nonprofits only -
pBAD/HisD-LSSmKate1
Plasmid#31908DepositorInsertLSSmKate1
TagsHISExpressionBacterialMutationK69Y/P131T/S148G/M167E/T183S/M196V compared to mK…Available SinceSept. 13, 2011AvailabilityAcademic Institutions and Nonprofits only -
pHG5
Plasmid#188253PurposeIntegrating plasmid contains the SV1 int/attP locus and the kanamycin-resistance gene (aphII) for selectionDepositorTypeEmpty backboneExpressionBacterialPromoteract1p/actII-orf4Available SinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1 3xFlag-ERK2
Plasmid#47573PurposeBacterial expression plasmid for GST-3xFlag-tagged ERK2DepositorAvailable SinceAug. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTKhc-yibDp-GFP-cp6
Plasmid#134938PurposeLow phosphate inducible circular permutation of GFPDepositorInsertGFPcp6
TagsHis tagExpressionBacterialAvailable SinceMay 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
TRBV24-1
Plasmid#123455Purposeconstruction of TCRDepositorAvailable SinceMay 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
TRBV6-2
Plasmid#123422Purposeconstruction of TCRDepositorAvailable SinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
TRBV4-3
Plasmid#123413Purposeconstruction of TCRDepositorAvailable SinceMay 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
TRAV39
Plasmid#123406Purposeconstruction of TCRDepositorAvailable SinceMay 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
TRAV10
Plasmid#123382Purposeconstruction of TCRDepositorAvailable SinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
TRAV9-2
Plasmid#123381Purposeconstruction of TCRDepositorAvailable SinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
TRAV8-6
Plasmid#123378Purposeconstruction of TCRDepositorAvailable SinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHis8-p100(1-899)
Plasmid#44735DepositorAvailable SinceJuly 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET22-HT/spoR
Plasmid#73631PurposeProduces Escherichia coli guanylate kinase (SpoR), N-terminal His6 tagDepositorInsertguanylate kinase
TagsHis6ExpressionBacterialPromoterT7Available SinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
SpyTag003-OspC
Plasmid#159998PurposeExpresses Borrelia burgdorferi OspC (20-209) C129S with N-terminal SpyTag003 fusion in bacterial cellsDepositorInsertSpyTag003-OspC (20-209) C129S
TagsHis6-tag and Thrombin cleavage siteExpressionBacterialMutationC129S mutation improves expression level.PromoterT7Available SinceNov. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
RhoC FLARE.sc mCer, mVenus - CA
Plasmid#65424PurposeRhoC CA biosensor single chain; mCerulean-tagged ROCK-RBD, mVenus-tagged RhoC Q63LDepositorInsertRBD, mCerulean, mVenus, RhoC
ExpressionBacterial, Insect, and Mamm…MutationT153M in mVenus, Q63L in RhoCAvailable SinceAug. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
RhoC FLARE.sc mCer, mVenus - DN
Plasmid#65423PurposeRhoC DN biosensor single chain; mCerulean-tagged ROCK-RBD, mVenus-tagged RhoC T19NDepositorInsertRBD, mCerulean, mVenus, RhoC
ExpressionBacterial, Insect, and Mamm…MutationT153M in mVenus, T19N in RhoCAvailable SinceAug. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEND-303_Cacnes_tetR
Plasmid#225618PurposeC. acnes with tetR-based inducible expression of mCherry. pBRESP36A with P(PPA_RS09745)+RBS_2+TetR // P(BBa_23119-TetO2)+RBS_1+mCherryDepositorInsertTetR
UseSynthetic BiologyTags6xHisExpressionBacterialMutationCodon optimized for Cutibacterium acnesAvailable SinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only