32,129 results
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Plasmid#221207PurposeScChd1-aa118-1274 chromatin remodelerDepositorInsertScChd1 (aa118-1274)
Tags6xHis-tag (Precission protease cleavable)ExpressionBacterialMutationdeleted amino acids 1-117 and 1275-1468Available SinceAug. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
B4-1
Plasmid#111475PurposeAla-tRNA synthetaseDepositorInsertAla-tRNA synthetase
Tags6xHisExpressionBacterialAvailable SinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX Syt1 C2AB (96-421)
Plasmid#170643PurposeExpression of syt1 for purification in E. ColiDepositorInsertSYT1 (Syt1 Rat)
ExpressionBacterialAvailable SinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
NLS-tdTomato
Plasmid#163443PurposeBacterial expression of His-tagged, SV40 NLS-tagged tdTomato in the modified pRSETB protein expression backboneDepositorInserttdTomato
Tags6xHisExpressionBacterialPromoterT7Available SinceJan. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSF4 TetCMV intron 20xGCN4 Renilla Rh1 STOP 24xMS2v5 SV40 CTE polyA
Plasmid#105000PurposeExpresses ST-Renilla-Rh1 calibration reporter in mammalian cells; note plasmid contains 24xGCN4 repeatsDepositorInsertRenilla luciferase
Tags24xGCN4 repeats (SunTag), 24xMS2v5 stem loops in …ExpressionBacterial and MammalianPromoterTetCMVAvailable SinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pVRb20_992
Plasmid#49714DepositorInsertsfgfp
UseSynthetic BiologyExpressionBacterialPromoterPecf20_992Available SinceJan. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pQLinkHD_ECFP
Plasmid#118857Purposefluorescent protein expression with an N-terminal His-tagDepositorInsertECFP
TagsHis-tagExpressionBacterialAvailable SinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-MBP-CBX1-eReader
Plasmid#135089PurposeBacterial expression of engineered CBX1 with enhanced binding to H3K9meDepositorInsertCBX1 chromobox protein 1, engineered
Tags6xhis-tag and MBPExpressionBacterialMutationlysine 43 mutated to alanine; aspartic acid 59 mu…PromoterT7Available SinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEVOL-AcKRS-CloDF
Plasmid#127415PurposeMutant of mmPylRS designed for incorporation of non-canonical amino acids (acyl-lysine derivatives) in to M13 bacteriophageDepositorInsertPyrrolysyl-tRNA synthetase/pyrrolysyl -tRNA pair
ExpressionBacterialPromoteraraBADAvailable SinceApril 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
819 pGEX2T PTEN C124S
Plasmid#10745DepositorAvailable SinceSept. 27, 2005AvailabilityAcademic Institutions and Nonprofits only -
pQE80L-SH3
Plasmid#160331PurposeThis plasmid encodes an engineered oxygen-independent flavin binding fluorescent protein with N-terminal 6xHis tag. Its thermostability and quantum yield is improved compared to wild type.DepositorInsertSH3
Tags6xHis tagExpressionBacterialPromoterT5Available SinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET His6 msfGFP TEV cloning vector with BioBrick polycistronic restriction sites (9GFP)
Plasmid#48287DepositorTypeEmpty backboneTagsHis6 and msfGFPExpressionBacterialAvailable SinceNov. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET16b-MYPT1(1-314)
Plasmid#224893PurposeBacterial expression of MYPT1(1-314)DepositorInsertmyosin phosphatase targeting subunit 1 (PPP1R12A )
ExpressionBacterialAvailable SinceNov. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pY2-MET-IV
Plasmid#218117PurposeMET-HYGRO plasmid (218133) with the functional cassettes of pY2 (218115) inserted at the sfGFP dropout locus.DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pY2-LYS-IV
Plasmid#218118PurposeLYS-Leu2 plasmid (218114) with the functional cassettes of pY2 (218115) inserted at the sfGFP dropout locus.DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBEL1284
Plasmid#175960PurposeFor expression of LetA-LetB operon from E. coli. N-terminal his tag on LetA, araBAD promoterDepositorInsertsLetA
LetB
Tags6xHis 2xQH and TEV cleavage site on LetAExpressionBacterialPromoteraraBADAvailable SinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-AR-S256A
Plasmid#171227PurposeMammalian expression of un-tagged human androgen receptor phosphorylation mutant: AR-Ser256Ala (AR-S256A)DepositorInserthuman androgen receptor Ser256Ala mutant (AR Human)
ExpressionBacterial and MammalianMutationAR-Ser256Ala (AR-S256A)PromoterCMVAvailable SinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-AR-S308A
Plasmid#171229PurposeMammalian expression of un-tagged human androgen receptor phosphorylation mutant: AR-Ser308Ala (AR-S308A)DepositorInserthuman androgen receptor Ser308Ala mutant (AR Human)
ExpressionBacterial and MammalianMutationAR-Ser308Ala (AR-S308A)PromoterCMVAvailable SinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-AR-S90A
Plasmid#171225PurposeMammalian expression of un-tagged human androgen receptor phosphorylation mutant: AR-Ser90Ala (AR-S90A)DepositorInserthuman androgen receptor Ser90Ala mutant (AR Human)
ExpressionBacterial and MammalianMutationAR-Ser90Ala (AR-S90A)PromoterCMVAvailable SinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDSG321
Plasmid#115598Purposesurface-displayed Nb3, used in composability experiments; medium copyDepositorInsertregulated adhesin construct
ExpressionBacterialAvailable SinceOct. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pET28-SpyCatcher002-MBP
Plasmid#216295PurposeExpresses SpyCatcher002 linked to maltose-binding protein in bacterial cellsDepositorInsertSpyCatcher002-MBP
TagsHis6 tag and TEV cleavage siteExpressionBacterialPromoterT7 promoterAvailable SinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
HS63 pComb3xss anti-Homer1 nanobody
Plasmid#134715PurposeBacterial expression of anti-Homer1 (Mouse) recombinant llama nanobody for use as an immunolabelDepositorInsertanti-Homer1 (Mouse) recombinant llama nanobody (Homer1 L. glama (llama))
Tags6xHis-HAExpressionBacterialPromoterlacAvailable SinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-SpyCatcher EQ
Plasmid#35045DepositorInsertSpyCatcher EQ
TagsHis6ExpressionBacterialMutationDeletion of C-terminal till I113, and point mutat…Available SinceApril 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pB2Hopt
Plasmid#163830PurposeTwo-hybrid system that confers spectinomycin resistance in response to substrate phosphorylation. Constitutive expression of c-src kinase, CDC37, and PTP1BDepositorInsertSpecR
UseSynthetic BiologyExpressionBacterialPromoterPro1;ProD;pLacZ-OptAvailable SinceJuly 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-41-4GS-LaG-2
Plasmid#172766PurposeBacterial expression of dimerized anti-GFP nanobodies LaG-41 and LaG-2 (4GS linker)DepositorInsertLaG-41-4GS-LaG-2
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available SinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTGD
Plasmid#88894PurposeTandem gene duplication cassette for RecA mediated homologous recombination in E. coliDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceMarch 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_TXK-1/1
Plasmid#91539PurposeProtein expression and purification of human SH3 domain construct TXK-1/1DepositorInsertTXK-1/1 (TXK Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_TEC-1/1
Plasmid#91252PurposeProtein expression and purification of human SH3 domain construct TEC-1/1DepositorInsertTEC-1/1 (TEC Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_ITK-1/1
Plasmid#91253PurposeProtein expression and purification of human SH3 domain construct ITK-1/1DepositorInsertITK-1/1 (ITK Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
YopH-pNIC-Bsa4
Plasmid#92063PurposepNIC expression vector encoding gene for YopHDepositorInsertYopH
Tags6x His TagExpressionBacterialMutationCodon optimized E. coliAvailable SinceJan. 17, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
PERK 4: PERKKD-pGEX4T-1
Plasmid#21817DepositorInsertPERK (537-1114) (Eif2ak3 Mouse)
TagsGST and Myc (9E10)ExpressionBacterialMutationPERK Kinase Domain. Amino acids 537-1114.Available SinceMarch 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
pRetroX-PTuner DD-M.EcoGII-v5-Centromeric protein C
Plasmid#122085PurposeExpress M.EcoGII fused to CENPC with destabilization domain - inducibleDepositorInsertDD-linker-M.EcoGII-V5-CENPC (CENPC E.coli, Human)
UseRetroviralTagsV5 tag on Nter of CENPCExpressionBacterial and MammalianMutationL341F, R930L (see comments below)Available SinceMarch 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHK316
Plasmid#235474PurposeInducible gene VIII by Ptet promoterDepositorInsertphage M13 geneVIII
ExpressionBacterialAvailable SinceMay 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAROTENE5X
Plasmid#208816PurposeEncodes bacterial crtEBIY operon under the control of synthetic promoter responsive to JUB1-derived artificial transcription factor in bacterial cellDepositorInsertFive copies of JUB1 binding site within the synthetic promoter
UseSynthetic BiologyExpressionBacterialPromoterJUB1 TF-responsive promoterAvailable SinceDec. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETM6-T7-VHH72
Plasmid#202487PurposeExpresses the nanobody VHH72 with T7 inductionDepositorInsertnanobody VHH72
Tags6XHisExpressionBacterialPromoterT7Available SinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDB179-LCC
Plasmid#218686PurposeEncodes codon optimized LCC enzyme for bacterial expressionDepositorInsertLCC
Tags10xHisExpressionBacterialAvailable SinceNov. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCgVchcrRNA
Plasmid#203815PurposeExpression of CRISPR RNA, shuttle vector for Corynebacterium glutamicum / E. coliDepositorInsertCRISPR(BsaI)
UseCRISPRExpressionBacterialAvailable SinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKB256
Plasmid#170039PurposeEncodes LacI-controlled expression of surface-displayed Bac2a and PvirK-mNeonGreen reporterDepositorArticleInsertsmNeonGreen
Bac2a
UseSynthetic BiologyTagsLpp, OmpA, and TetherExpressionBacterialPromoterPtac and PvirKAvailable SinceJuly 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTA-attP
Plasmid#18939DepositorInsertphiC31 attP
ExpressionBacterialAvailable SinceOct. 17, 2008AvailabilityAcademic Institutions and Nonprofits only