29,754 results

1. pQLinkG2

   Plasmid

   #13671

   Depositor

   Konrad Buessow

   Article

   Scheich et al Nucleic Acids Res. 2007 Feb 20. ():.

   Insert

   multi cloning site
   

   Use

   Co-expression

   Tags

   GST, TEV, and attB1

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 6, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBFC0996

   Plasmid

   #186695

   Purpose

   VcDART under control of Lac promoter, Vc_2xBsaI_NT gRNA, 2xLguI cargo stuffer, with ET-Seq (AsiSI+SbfI) restriction sites flanking Tn7 Right End

   Depositor

   Jennifer Doudna

   Article

   Rubin et al Nat Microbiol. 2022 Jan;7(1):34-47. doi:

   Inserts

   tnsA
   tnsB
   tnsC
   tnsD
   tniQ
   cas8-cas5 fusion
   cas7
   cas6
   crRNA
   Tn7 transposon
   2xLguI Cargo Stuffer
   SbfI+AsiSI dual restriction site for donor plasmid removal during ET-Seq
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PLac

   Available since

   Sept. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEX-4T1 SHP2 WT

   Plasmid

   #8322

   Depositor

   Ben Neel

   Article

   O'Reilly et al Mol Cell Biol 2000 Jan;20(1):299-311.

   Insert

   SHP2 (PTPN11 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 14, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET24a MDH_AQUAR

   Plasmid

   #204239

   Purpose

   Bacterial expression of A. arcticum malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).

   Depositor

   Malate dehydrogenase CUREs Community (MCC)

   Insert

   MDH_AQUAR
   

   Use

   Tags

   6X His

   Expression

   Bacterial

   Mutation

   Promoter

   Lac

   Available since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. His-Tev-Nedd4L

   Plasmid

   #186806

   Purpose

   Codon-optimized bacterial expression plasmid. Expresses ubiquitin ligating enzyme His-Tev-Nedd4L.

   Depositor

   Lalit Deshmukh

   Article

   Nelson et al J Am Chem Soc. 2023 Apr 3. doi: 10.1021/

   Insert

   His-Tev-Nedd4L (NEDD4L Human)
   

   Use

   Tags

   His6-Tev

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. ASSA

   Plasmid

   #42368

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   ASSA (ASS1 Human)
   

   Use

   Tags

   His6-TEV

   Expression

   Bacterial

   Mutation

   See Comments

   Promoter

   Available since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

7. pSLS.400

   Plasmid

   #187201

   Purpose

   Expresses retron Eco4 RT

   Depositor

   Seth Shipman

   Article

   Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:

   Insert

   Retron Eco4 Reverse Transcriptase
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   mphR

   Available since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSET-dCas9

   Plasmid

   #110183

   Purpose

   Expression of the dCas9 gene in Streptomyces

   Depositor

   Yinhua Lu

   Article

   Zhao et al Biotechnol J. 2018 Jun 3. doi: 10.1002/b

   Insert

   dCas9
   

   Use

   CRISPR; I

   Tags

   Expression

   Bacterial

   Mutation

   D10A, H840A

   Promoter

   ermE*p

   Available since

   July 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCE27

   Plasmid

   #174405

   Purpose

   C. auris LEUpOUT NAT marker gRNA expression construct. Use with pCE35 CAS9 expression construct.

   Depositor

   Clarissa Nobile

   Article

   Ennis et al Microbiol Spectr. 2021 Dec 22;9(3):e0182

   Insert

   NAT 2 of 2, pSNR52, ADE2 gRNA, gRNA conserved, C. auris LEU2 2 of 2
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pConRef-2H12

    Plasmid

    #221182

    Purpose

    cdGreen expressed from a constitutive promoter in an operon with mScarlet-I (downstream) as a reference FP (Internal Lab ID: UJ11213)

    Depositor

    Urs Jenal

    Article

    Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

    Inserts

    cdGreen
    mScarlet-I
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N-terminus changed: starts with MSKKYGEAVIKE ...

    Promoter

    Constitutive and None (same as cdGreen)

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKESK22

    Plasmid

    #64857

    Purpose

    expression vector, low to medium copy number, tac promoter lacI, kanamycin resistance, p15A origin of replication

    Depositor

    Karin Schnetz

    Article

    Stratmann et al J Bacteriol. 2008 Feb;190(3):926-35. Epu

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Ptac

    Available since

    April 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCE38

    Plasmid

    #174432

    Purpose

    C. auris LEUpOUT HYG marker CAS9 expression construct. Use with pCE41 gRNA expression construct.

    Depositor

    Clarissa Nobile

    Article

    Ennis et al Microbiol Spectr. 2021 Dec 22;9(3):e0182

    Insert

    C. auris LEU2 1 of 2, pENO1, Cas9, HYG 1 of 2
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCE41

    Plasmid

    #174433

    Purpose

    C. auris LEUpOUT HYG marker gRNA expression construct. Use with pCE38 CAS9 expression construct.

    Depositor

    Clarissa Nobile

    Article

    Ennis et al Microbiol Spectr. 2021 Dec 22;9(3):e0182

    Insert

    HYG 2 of 2, pSNR52, ADE2 gRNA, gRNA conserved, C. auris LEU2 2 of 2
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Dec. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGJJ001

    Plasmid

    #183619

    Purpose

    bindingPCA plasmid - Contains a barcode landing pad and two fragments of the murine methotrexate-resistant DHFR under the expression of two CYC promoters.

    Depositor

    Ben Lehner

    Article

    Faure et al Nature. 2022 Apr;604(7904):175-183. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    May 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPP089

    Plasmid

    #158796

    Purpose

    pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged CasΦ-3 in MCS1 for expression in E. coli and protein purification

    Depositor

    Jennifer Doudna

    Article

    Pausch et al Science. 2020 Jul 17;369(6501):333-337.

    Insert

    CasPhi-3
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEX-4T1-14-3-3 epsilon GST

    Plasmid

    #13279

    Depositor

    Michael Yaffe

    Article

    Yaffe et al Cell. 1997 Dec 26. 91(7):961-71.

    Insert

    14-3-3 epsilon (Ywhae Rat)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 7, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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17. bdSUMO-GFP-HiBit

    Plasmid

    #170989

    Purpose

    For bacterial expression of bdSUMO tagged muGFP with NanoLuc complementation peptide variant #86

    Depositor

    Angus Johnston

    Article

    Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

    Insert

    14x-His-bdSUMO-muGFP-HiBiT
    

    Use

    Luciferase

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pOmpF

    Plasmid

    #42606

    Depositor

    Bryan Berger

    Article

    Su et al Protein Sci. 2013 Jan 24. doi: 10.1002/p

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 22, 2013

    Availability

    Academic Institutions and Nonprofits only

19. pHimar6

    Plasmid

    #137081

    Purpose

    bacterial Himar1 mini-transposon donor plasmid

    Depositor

    Harris Wang

    Article

    Chen et al CRISPR J. 2019 Dec;2(6):376-394. doi: 10

    Insert

    Himar1 mini-transposon with chloramphenicol resistance gene
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. p15aC-4D1D-5

    Plasmid

    #107866

    Purpose

    Bacterial expression of PIS, PI4K and PI4P5K

    Depositor

    Sanford Simon

    Article

    Botero et al Sci Adv. 2019 Mar 27;5(3):eaat4872. doi:

    Inserts

    PIS
    phosphatidylinositol 4-phosphate 5-kinase
    phosphatidylinositol 4-kinase β
    

    Use

    Tags

    myc

    Expression

    Bacterial

    Mutation

    Promoter

    proD and proD (in operon)

    Available since

    April 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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