30,753 results

1. pYTRW26K_1Ti1

   Plasmid

   #177292

   Purpose

   genomic integration of genes (inserted in I-SceI-site) via rrn-interposon/SacB with TcR and eYFP

   Depositor

   Karl-Erich Jaeger

   Article

   Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

   Insert

   PsacB-sacB
   

   Use

   Synthetic Biology; Yeast expression, rrn genomic …

   Expression

   Bacterial

   Available Since

   Aug. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDWJ26

   Plasmid

   #207603

   Purpose

   GFP-AIA expression

   Depositor

   Colin Kleanthous

   Article

   Williams-Jones et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

   Insert

   GFP-AIA
   

   Expression

   Bacterial

   Available Since

   Nov. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSHDY-Prha-mVenus_rhaS

   Plasmid

   #137662

   Purpose

   Conjugative vector derived from pSHDY. Constitutive expression of the activator rhaS, as well as rhamnose-inducible expression of mVenus.

   Depositor

   Ilka Axmann

   Article

   Behle et al ACS Synth Biol. 2020 Apr 17;9(4):843-855

   Insert

   Prha:mVenus
   

   Expression

   Bacterial

   Mutation

   rhaS (Arg218Leu)

   Promoter

   Prha

   Available Since

   Feb. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMan operon

   Plasmid

   #124888

   Purpose

   Expresses proteins encoding Man operon in E. faecalis

   Depositor

   Kazuhisa Sekimizu

   Article

   Matsumoto et al Commun Biol. 2019 May 2;2:157. doi: 10.1

   Insert

   manX1, manX2, manY, manZ, manO, EF0025
   

   Expression

   Bacterial

   Available Since

   April 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. p3R112C/T180CBM3

   Plasmid

   #85090

   Purpose

   Expresses PCNA3 variant (R112C/T180C) fused to P450 BM3 variant (A74G/C773S/C810S) in E. coli

   Depositor

   Teruyuki Nagamune

   Article

   Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

   Insert

   The R112C/T180C variant of PCNA3 fused to P450 BM3
   

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   R112C/T180C in PCNA3, A74G/C773S/C810S in P450BM3

   Promoter

   T7 promoter

   Available Since

   Dec. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHH0103_GNAI1_G-alpha

   Plasmid

   #110012

   Purpose

   Protein expression and purification of GNAI1_G-alpha

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

   Insert

   GNAI1_G-alpha (GNAI1 Human)
   

   Expression

   Bacterial

   Available Since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. ADA

   Plasmid

   #39020

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   ADA (ADA Human)
   

   Tags

   TEV-His6-Flag

   Expression

   Bacterial

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

8. TwinStrep-SUMO-NLS-BhCas12b_v4

   Plasmid

   #158615

   Purpose

   For protein expression of BhCas12b_v4

   Depositor

   Feng Zhang

   Article

   Strecker et al Nat Commun. 2019 Jan 22;10(1):212. doi:

   Insert

   NLS-BhCas12b_v4
   

   Tags

   6xHIS-TwinStrep-SUMO

   Expression

   Bacterial

   Available Since

   Aug. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCoofy44

   Plasmid

   #55185

   Purpose

   Insect expression vector for parallel SLIC cloning containing N-terminal 10x His and SumoStar tags. C-terminal tags can be added during SLIC.

   Depositor

   Sabine Suppmann

   Article

   Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

   Type

   Empty backbone

   Tags

   10x His, 10x His-Stop-OneStrep-Stop-S-tag-Stop-HP…

   Expression

   Bacterial

   Promoter

   pPH (Polyhedrin promoter)

   Available Since

   July 23, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEvoltac-EcW-TGA-h14

    Plasmid

    #218764

    Purpose

    encodes EcWRS-h14 and EcW-tRNA-TGA

    Depositor

    Abhishek Chatterjee

    Article

    Italia et al Nat Chem Biol. 2017 Apr;13(4):446-450. d

    Insert

    EcWRS-h14
    

    Expression

    Bacterial

    Mutation

    S8A, V144G, V146C

    Promoter

    tacI

    Available Since

    July 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGEX-2T-Rac1-wt

    Plasmid

    #12977

    Depositor

    Gary Bokoch

    Insert

    Rac1 (RAC1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    Oct. 27, 2006

    Availability

    Academic Institutions and Nonprofits only

12. pKTrfp

    Plasmid

    #59772

    Purpose

    pKT230 derivative; PBAD_rfp; cmR

    Depositor

    Nathan Hillson

    Article

    Bi et al Microb Cell Fact. 2013 Nov 13;12:107. do

    Insert

    rfp
    

    Expression

    Bacterial

    Promoter

    pBAD

    Available Since

    Sept. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET11a-4SS-HEWL

    Plasmid

    #39233

    Depositor

    Harald Schwalbe

    Article

    Schlorb et al J Biomol NMR. 2005 Oct;33(2):95-104.

    Insert

    Hen egg white lysozyme (LYZ Chicken)
    

    Expression

    Bacterial

    Mutation

    N-terminal Met

    Promoter

    T7

    Available Since

    Sept. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJCSUP35

    Plasmid

    #1088

    Depositor

    Susan Lindquist

    Article

    Glover et al Cell 1997 May 30;89(5):811-9.

    Insert

    SUP35 (SUP35 Budding Yeast)
    

    Tags

    HIS tag

    Expression

    Bacterial

    Available Since

    May 26, 2005

    Availability

    Academic Institutions and Nonprofits only

15. LacI-pLacI-pIV pm

    Plasmid

    #218101

    Purpose

    Phagemid that expresses LacI, and pIV under the Lac promoter

    Depositor

    Kevin Esvelt

    Insert

    pIV
    

    Expression

    Bacterial

    Promoter

    Lac promoter

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCoofy9

    Plasmid

    #122004

    Purpose

    E.coli vector for parallel SLIC cloning containing N-terminal StrepII with PreScission Cleavage site

    Depositor

    Sabine Suppmann

    Article

    Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

    Type

    Empty backbone

    Tags

    StrepII

    Expression

    Bacterial

    Available Since

    May 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pccGFPKAN

    Plasmid

    #73649

    Purpose

    Fluorescent reporter for transmembrane protein dimerization.

    Depositor

    Alessandro Senes

    Article

    Armstrong et al Biochim Biophys Acta. 2016 Jul 21. pii:

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pUC18T-mini-Tn7T-hph-Para

    Plasmid

    #218363

    Purpose

    Empty backbone for Tn7-site insertion with araC-ParaBAD expression system

    Depositor

    Michael Gebhardt

    Article

    Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    June 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSR1037

    Plasmid

    #208736

    Purpose

    Characterization plasmid for PSarA1 (sfgfp expressed from PSarA1 on the S. aureus pC194 replicon)

    Depositor

    Lauren Andrews

    Article

    Rondthaler et al ACS Synth Biol. 2023 Dec 8. doi: 10.1021

    Insert

    PSarA1
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Dec. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. ELAC1

    Plasmid

    #39097

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    ELAC1 (ELAC1 Human)
    

    Tags

    Tev His Flag

    Expression

    Bacterial

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only