32,161 results
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Plasmid#187390Purposearabinose-inducible expression of GFP and mCherryDepositorInsertGFP, mCherry
ExpressionBacterialAvailable SinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAGM22082_cRed
Plasmid#117225PurposepET-28b backbone plasmid, Level 2 Golden Gate expression plasmid.DepositorInsertCanthaxanthin Operon
Tags34 amino acid N-terminal linker, Hexahistidine Ta…ExpressionBacterialMutationremoved Type IIS restriction sites within the bac…PromoterT7 RNA PolymeraseAvailable SinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGST-PPARgamma
Plasmid#16549DepositorInsertPPAR gamma (PPARG Human)
TagsGSTExpressionBacterialMutationN-terminal DNA-binding domain: aa 1-248Available SinceMarch 24, 2008AvailabilityAcademic Institutions and Nonprofits only -
pBad33_ISTfu1_reRNA
Plasmid#205355PurposeBacterial expression of ISTfu1 TnpB and its reRNADepositorInsertISTfu1 TnpB
ExpressionBacterialAvailable SinceOct. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
5 pCOLI_A_STREP
Plasmid#160147PurposeN-term 2xStrepII 3C cleavable Amp resistant bacterial expression vectorDepositorTypeEmpty backboneTags2xStrepIIExpressionBacterialPromoterT7Available SinceNov. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28-Dnmt3a3L-sc27
Plasmid#71827PurposeFor bacterial expression of Dnmt3a-Dnmt3L single-chain fusion protein protein. Contains the catalytic domain of mouse Dnmt3a and the C-terminal part of mouse Dnmt3L connected by 27 aa linker.DepositorInsertDnmt3a catalytic domain (M. musculus) Dnmt3L (M. musculus) C-terminal domain gene fusion (Dnmt3a Mouse)
Tags6xHis tagExpressionBacterialAvailable SinceJan. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAWPSG-Po-nCas9-BE- Ptac-sgRNA(mmoX_1)
Plasmid#195741PurposeBase editing for making a pre-stop codon in mmoX using phenol inducible systemDepositorArticleInsertDi-Methyl Phenol Regulatory protein / APOBEC1-nCas9(D10A)-UGI / Ptac-RiboJ-sgRNA(mmoX_1)
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterPo (phoenol-inducible promoter)Available SinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
Parp14-c012
Plasmid#173099PurposeExpression of recombinant fusion protein in E. coliDepositorAvailable SinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
H2B-2A-GFP-IRESpuro2
Plasmid#183746PurposeExpresses H2B-2A-GFPDepositorInsertH2B (H2BC16P Human)
TagsGFP with 2A protease cleavage site between H2B an…ExpressionBacterial and MammalianPromoterCMVAvailable SinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET26b_AT118-L-V5-His
Plasmid#222848PurposeE. coli periplasmid expression plasmid encoding Nb. AT118-L, a humanized nanobody antagonist of the human Angiotensin II type I receptor (AT1R) with reduced polyreactivityDepositorInsertAT118-L
TagsV5-HisExpressionBacterialAvailable SinceSept. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX Su(fu) (CT#99)
Plasmid#13856DepositorInsertSu(fu) (SUFU Chicken)
TagsGSTExpressionBacterialMutationNco I fragment from full length; ~2kbp- ATG to st…Available SinceFeb. 9, 2007AvailabilityAcademic Institutions and Nonprofits only -
pFN18A-HaloTag-SpyCatcher
Plasmid#206041PurposeHaloTag-Spy0128-(Protein of Interest)-Spy0128-SpyCatcher-8xHisTagDepositorTypeEmpty backboneTagsHaloTag and SpyCatcher-8HisExpressionBacterialPromoterT7Available SinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-His-VCre
Plasmid#123131PurposeBacterial expression plasmid for wild-type VCre with an N-terminal His-tag.DepositorInsertVCre recombinase
UseCre/LoxTags6x His tagExpressionBacterialPromoterT7Available SinceApril 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDM_T7_HisLacZomega
Plasmid#118815PurposeT7 RNAP-driven expression of N-terminal His-tagged lacZ omega subunit.DepositorInsertlacZ_
TagshistagExpressionBacterialAvailable SinceAug. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV4Neo-murine IL-17RA-HA
Plasmid#46859Purposeexpresses mouse IL-17RADepositorAvailable SinceSept. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET-2(KW)2-eGFP
Plasmid#112736PurposeExpress 2(KW)2-eGFP in E. coliDepositorInsert2(KW)2-eGFP
Tags6xHisExpressionBacterialPromoterT7 PromoterAvailable SinceJuly 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
human p53-(311-393)
Plasmid#24869DepositorAvailable SinceAug. 5, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCRIS
Plasmid#120424PurposeThis plasmid encodes the complete CRISPR/dCas9 machinery for repressing transposition of the following bacterial Insertion Sequences: IS1, IS3, IS5, and IS150.DepositorInsertCRISPR spacers targeting IS1, IS5, IS3, IS150
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterconstitutiveAvailable SinceJan. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28 Set9 wt
Plasmid#24082DepositorAvailable SinceOct. 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
PANK3
Plasmid#32872PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertPANK3 (PANK3 Human)
TagsHis tag (with Thrombin cleavage)ExpressionBacterialMutationamino acids 10-370 onlyAvailable SinceJan. 6, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCMV-EPN-24
Plasmid#79939PurposePH-Late1-I3-01-mycDepositorInsertEPN-24
ExpressionBacterial and MammalianAvailable SinceFeb. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJMP1183
Plasmid#119254Purposerfp "test" strainDepositorInsertsgRNA NT1/RR1 (rfp)
ExpressionBacterialMutationD10A and H840AAvailable SinceJan. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
Terminase_PhiV1
Plasmid#188836PurposeLarge terminase from phage PhiV1DepositorInsertTerminase
ExpressionBacterialAvailable SinceAug. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFW6000
Plasmid#224215PurposeBacteroides genomic editing tool; aTc inducible-Fncpf1DepositorInsertFncpf1
UseCRISPR and Synthetic BiologyExpressionBacterialAvailable SinceSept. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET22b-TEVP-cleavableELP
Plasmid#219402PurposeTobacco etch virus protease-responsive elastin-like polypeptide (ELP)DepositorInsertTEVP-cleavableELP
Tags6xHisExpressionBacterialAvailable SinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWUKI 1+2
Plasmid#79879PurposeEncodes Cas1+2 and a CRISPR arrayDepositorInsertsCas1
Cas2
CRISPR Array
ExpressionBacterialPromoterT7Available SinceOct. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBzCas13b-gRNA-1
Plasmid#164858PurposeConstitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for BzCas13b in bacteria.DepositorInsertBzCas13b-gRNA-1
ExpressionBacterialPromoterJ23119Available SinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag4A-MMP8 Wt
Plasmid#29545DepositorAvailable SinceMay 23, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGI3EM17
Plasmid#210569PurposeReporter gene expression in B. dendrobatidisDepositorInsertSpH2Bpro-Hshph-G4S-nanoluc-ScADH1ter
UseLuciferaseTagsNanoLuc luciferaseExpressionBacterial and YeastPromoterSpH2BproAvailable SinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSET-pr-Kaede (Kaede-A69T)
Plasmid#99228PurposeBacterial expression of the primed conversion capable protein pr-Kaede (Kaede-A69T).DepositorInsertpr-Kaede
ExpressionBacterialMutationA69T for primed conversion applicationsPromoterT7Available SinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only