31,059 results
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pSB3C5-phzSM
Plasmid#141105PurposeContains a phzS-phzM operon BioBrickDepositorInsertphzSM
ExpressionBacterialAvailable SinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
T1-araC-Pbad-D10A/H840A cas9 ABCD-pACYC-BB
Plasmid#110547PurposeCRISPR interference; works in conjunction with an sgRNA expression vector to silence genes in the E. coli chromosome.DepositorInsertdCas9
ExpressionBacterialMutationD10A, H840APromoterpBADAvailable SinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJKW0770
Plasmid#119023PurposeE.coli expression plasmid for PmSPS1DepositorInsertPmSPS1
Tags8xHisExpressionBacterialPromoterT7Available SinceJune 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
PARP1-c009
Plasmid#216872PurposeBacterial expression vector for E. coli producing PARP1 catalytic protein (without Regulatory domain); with N-terminal CFP fusionDepositorInsertPARP1 catalytic domain (no Regulatory Domain) (PARP1 Human)
ExpressionBacterialAvailable SinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNIC-HIS6-GST-TEV-GG
Plasmid#215801PurposeCloning of target gene with N terminal His6 GST tag and TEV cleavageDepositorTypeEmpty backboneExpressionBacterialAvailable SinceApril 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
PTGDSA
Plasmid#42452PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pOPS0263
Plasmid#133228PurposeReporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.DepositorInsertconstructed with PsNifH (pOGG043), gusA (pOGG083) and T-pharma (pOGG003)
ExpressionBacterialMutationDomesticated for Golden Gate cloningAvailable SinceApril 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOPS0262
Plasmid#133227PurposeReporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.DepositorInsertconstructed with PsNifH (pOGG043), celB (pOGG050) and T-pharma (pOGG003)
ExpressionBacterialMutationDomesticated for Golden Gate cloningAvailable SinceApril 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pKSS
Plasmid#39272DepositorTypeEmpty backboneUsePositive-selection cloning vectorExpressionBacterialAvailable SinceSept. 4, 2012AvailabilityAcademic Institutions and Nonprofits only -
RFP-(SIM)1
Plasmid#127094PurposeBacterial expression plasmid for mCherry-(SIM)1 client to assess SUMO-SIM scaffolds.DepositorInsertmCherry - SUMO interacting motif (SIM) from PIASx (PIAS2 Human)
TagsmCherryExpressionBacterialPromoterunknownAvailable SinceAug. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCfuNca-Plastid
Plasmid#190818PurposePlastid targeting GFP with ble resistant gene for zeocinDepositorInsertsDiatom plastid targeted GFP
Bleomycin resistance gene
ExpressionBacterialPromoterCylindrotheca fusiformis fcp and Nitzschia captiv…Available SinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLITMUS*-PrpoN-Cro-PBBa_J23106-geneIII
Plasmid#134353PurposePhagemidDepositorInsertCro
ExpressionBacterialAvailable SinceJune 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-EPN-11
Plasmid#79926PurposePalm1-Late1-I3-01-mycDepositorInsertEPN-11
ExpressionBacterial and MammalianAvailable SinceFeb. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX-PAK2 wt
Plasmid#31671DepositorAvailable SinceApril 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
pJL1-mTFP1
Plasmid#102635PurposeIn vitro expression of mTFP1 from the T7 promoterDepositorInsertmTFP1
ExpressionBacterialPromoterT7Available SinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-PA-Rac1-I539E
Plasmid#22026PurposeExpression of photoactivatable Rac1 ('lit' state mutant)DepositorInsertPA-Rac1-I539E (RAC1 Avena sativa (oat), Human)
Tags6X HisExpressionBacterial, Insect, and Mamm…MutationLOV domain contains 'lit state' mutatio…PromoterCMV, p10Available SinceSept. 29, 2009AvailabilityAcademic Institutions and Nonprofits only -
pSARSF53-110
Plasmid#61813PurposeTvoVMAN11 inteinDepositorInsertTvoVMAN11 intein
TagsH6-Smt3ExpressionBacterialPromoterT7Available SinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSABAD14-98
Plasmid#61827PurposeTvoVMAdN11 inteinDepositorInsertTvoVMAdN11 intein
TagsGB1-H6ExpressionBacterialPromoterpBADAvailable SinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
PE_26: Para_Lac_Tet_eG::sfYFP
Plasmid#104887PurposePE_26: Para_Lac_Tet_eG::sfYFPDepositorInsertHybrid Promoter Para_Lac_Tet_eG
ExpressionBacterialPromoterPara_Lac_Tet_eGAvailable SinceJan. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOPINK-OTUD3 (OTU+UBA, aa 52-275)
Plasmid#61411PurposeExpresses human OTUD3 (OTU+UBA domains) in E. coli.DepositorInsertOTUD3 (OTUD3 Human)
TagsHis6-GST-3CExpressionBacterialMutationDeleted aa 1-51 and aa 276-398.PromoterT7Available SinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
Sc eRF3∆165N (WT)
Plasmid#175418PurposeEncodes His-MBP-TEV-WT eRF3∆165NDepositorAvailable SinceOct. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAJM.716
Plasmid#108518PurposePBAD-YFP Reporter. Ara sensor for use in Marionette strain.DepositorInsertPBAD-YFP
ExpressionBacterialAvailable SinceAug. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
YWHAG
Plasmid#39129PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pJKW0957
Plasmid#119026PurposeE.coli expression plasmid for PmKOMT1DepositorInsertPmKOMT1
Tags8xHisExpressionBacterialPromoterT7Available SinceJune 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28-MRPP1
Plasmid#67862Purposebacterial expression of TRMT10C, full coding sequence (mitoch. form) + N-term. His tag (thrombin site)DepositorInsertMRPP1 (TRMT10C Human)
TagsHisExpressionBacterialMutationmitoch. form (contains aa 40-403)PromoterT7Available SinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
ALC1-c007
Plasmid#173112PurposeExpression of recombinant fusion protein in E. coliDepositorAvailable SinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBC155
Plasmid#202274PurposeLevel 2 for strain-specific barcode DNA tag with bc182 through Tn7 bacterial insertion, Spectinomycin and Streptomycin selectable marker and tagBFP fluorescent markerDepositorInsertbc182
ExpressionBacterialAvailable SinceApril 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBC114
Plasmid#202276PurposeLevel 2 for strain-specific barcode DNA tag with bc141 through Tn7 bacterial insertion, Tetracyline selectable marker and far-red-FP fluorescent marker mPlumDepositorInsertbc141
ExpressionBacterialAvailable SinceApril 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp-LZ_VNp-LZ-mNeongreen-hGH
Plasmid#182425PurposeBacterial expression of Vesicle Nucleating peptide-Leucine Zipper and Vesicle Nucleating peptide-Leucine Zipper-mNeongreen-human growth hormone fusion proteinsDepositorInsertsTagsVesicle Nucleating peptide (VNp) and Vesicle Nucl…ExpressionBacterialPromoterT7Available SinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pACYC-PLlambda-alkS(WT)
Plasmid#44382DepositorInsertAlkS(WT)
UseSynthetic BiologyExpressionBacterialPromoterPLlambdaAvailable SinceJuly 15, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBTK510
Plasmid#110611PurposeBTK assembled broad-host-range plasmid encoding GFP driven by PA3 promoterDepositorInsertGFP
ExpressionBacterialAvailable SinceAug. 30, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMRE146
Plasmid#118498Purposebroad-host range plasmid to constitutively express fluorescent protein genes in bacteriaDepositorInsertmCardinal
ExpressionBacterialAvailable SinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEB1-mClover3
Plasmid#103994PurposePlasmid encoding mClover3DepositorInsertmClover3
UseLow copyExpressionBacterialMutationMutations relative to wild-type GFP (S30R, Y39N, …PromoterproCAvailable SinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
p35u4
Plasmid#174786PurposeConstitutive expression of λCI fused via three alanines to the MS2 coat protein. Useful as RNA-DNA adapter in three-hybrid systemDepositorInsertMS2 coat protein
TagsλCIExpressionBacterialMutationV30I, A81G, positions 68-80 deleted (VATQTVGGVELP…PromoterSynthetic Constitutive PromoterAvailable SinceMay 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET42a-lambdaN+-L+-GSH
Plasmid#98894PurposeExpresses lambdaN+-L+-GST fusion protein for affinity purification with ARiBo methodDepositorInsertlambdaN+-L+
TagsGST tagExpressionBacterialPromoterT7 promoterAvailable SinceAug. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSb_init containing anti SARS-CoV-2_RBD sybody Sb#15
Plasmid#153523PurposeExpression of the anti SARS-CoV-2_RBD sybody Sb#15DepositorInsertSb#15
ExpressionBacterialPromoterpBADAvailable SinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBIG1b_10xHisTEVATG5_ATG12
Plasmid#190861PurposePlasmid for the expression of ATG5 and ATG12 protein subunit complexes. Internal References: SMC1178DepositorAvailable SinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
ReactionC_pBIG1a_ATG7_ATG10
Plasmid#190860PurposePlasmid for the expression and purification of ATG7 and ATG10 protein subunit complexes. Internal reference: SMC1179DepositorAvailable SinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only
