32,656 results
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Plasmid#215769Purposeencodes for HDR template for optimized AsCas12a-mediated knock-in of a CD19-specific CAR into the TRAC locusDepositorInsertsExpressionBacterialMutationCas12a PAM mutatedAvailable SinceJune 5, 2024AvailabilityAcademic Institutions and Nonprofits only
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His-MBP_G4G6 in pRSF
Plasmid#188454PurposeExpress in E. coli human gelsolin segments 4 through 6 with N-terminal 6xHis and MBP tags.DepositorAvailable SinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLC-242
Plasmid#73189PurposeTemplate plasmid which encodes chloramphenicol resistance gene for positive selection and a toxin gene (relE) under the control of rhamnose induceable promoter (PrhaB) for negative selection.DepositorInsertchloramphenicol (cat Although Ampicillin gene is present, it was inactivated while cloning.)
ExpressionBacterialPromoterchloramphenicol promoterAvailable SinceJuly 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pOPINK-OTUD3 (OTU, aa 52-209)
Plasmid#61412PurposeExpresses human OTUD3 (OTU domain) in E. coli.DepositorInsertOTUD3 (OTUD3 Human)
TagsHis6-GST-3CExpressionBacterialMutationDeleted aa 1-51 and aa 210-398.PromoterT7Available SinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-MCN-10His-StayGold
Plasmid#211360PurposeBacterial expression of the bright and photostable StayGold fluorescent protein. Codon optimised for expression in Escherichia coli. Contains a N-terminal 10xHis tag.DepositorInsertStayGold
Tags10xHis-tagExpressionBacterialPromoterT7Available SinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBJ6
Plasmid#167135PurposeA gentamycin resistant plasmid for Tn7-mediated chromosomal integration of Photorhabdus luminescens luxCDABE operon with Pkan promoterDepositorInsertluxCDABE
UseLuciferaseExpressionBacterialPromoterKanamycin promoterAvailable SinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAD-LyseR (in lacZ deficient strain)
Plasmid#99245PurposeFor autolysate production in lacZ deficient (lac operon deletion) E. coli BL21-Gold-dLac (DE3) strain: constitutively expresses phage lambda endolysin (gene R)DepositorAvailable SinceOct. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSNAP-CLC-SNAP
Plasmid#38012DepositorInsertClathrin, light polypeptide (LCa) (Clta Mouse)
TagsSNAP26mExpressionBacterial and MammalianPromoterCMVAvailable SinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
plRL118
Plasmid#163632PurposeRenilla luciferase reporter plasmid; Giles integrating.DepositorTypeEmpty backboneUseLuciferaseExpressionBacterialPromoterMycobacterial Optimized Promoter (MOP)Available SinceAug. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSKBAD2
Plasmid#15335DepositorInsertDnaE C-intein
TagsGB1ExpressionBacterialAvailable SinceDec. 14, 2007AvailabilityAcademic Institutions and Nonprofits only -
pMVV099
Plasmid#215305PurposeExpresses sfGFP under induction with the Cuminic Acid Marionette Cassette (CymRAM + PCymRC-sfGFP) with a BBR1 backbone.DepositorInsertsfGFP
UseSynthetic BiologyTagsNoneExpressionBacterialPromoterPCymRCAvailable SinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
ShadowG-SUMO1
Plasmid#175239PurposeBacterial expression and purification, conjugatable SUMO for SUMOylationDepositorAvailable SinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRF-SrpR
Plasmid#49372DepositorInsertSrpR repressor
UseSynthetic BiologyExpressionBacterialAvailable SinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDR111_Bgal no NLS
Plasmid#188397PurposeExpresses beta-galactosidase via Phyper-spank which is optimized for Bacillus subtilis. There is a secretion peptide (PhoD) that is fused to beta-galactosidase.DepositorInsertlacZ
TagsPhoD secretion peptideExpressionBacterialPromoterPhyper spankAvailable SinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFP.E227
Plasmid#138249PurposeReporter plasmid - Expresses three fluorescent reporters from three different ECF promotersDepositorInsertsmCherry
Terminator
mTagBFP
Terminator
gfp
Terminator
UseSynthetic BiologyTags6xHisExpressionBacterialPromoterP17_up1691, P20_992, and iP16_3622Available SinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHCL149
Plasmid#134457PurposeCmR, Para-popZ-rbs-H3H4-msfGFPN-CTM. Replicative plasmid for the expression of bait. N-terminal GFP fusion.DepositorInsertPara-popZ-rbs-H3H4-msfGFPN-CTM.
ExpressionBacterialPromoterParaAvailable SinceJan. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOPINB-OTUB1*
Plasmid#65441PurposeExpresses activated OTUB1 in E. coliDepositorTagsHis-3CExpressionBacterialMutationActive site cysteine mutated to alaninePromoterT7Available SinceJuly 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGJJ045
Plasmid#183621PurposeabundancePCA plasmid - Contains a barcode landing pad and two fragments of the murine methotrexate-resistant DHFR, the expression of one driven by the CYC promoter and the other by the GPD promoter.DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceMay 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNIC-HIS6-THRX-TEV-GG
Plasmid#215796PurposeCloning of target gene with N terminal His6 Thioredoxin tag and TEV cleavageDepositorTypeEmpty backboneExpressionBacterialAvailable SinceApril 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
PJEBAN3
Plasmid#203682PurposeExpresses Yellow Fluorescent Protein (YFP) in Listeria monocytogenesDepositorInsertYellow Fluorescent Protein (YFP)
ExpressionBacterialAvailable SinceSept. 13, 2023AvailabilityAcademic Institutions and Nonprofits only