30,803 results

1. pcDNA3.1/V5-His-mPer1

   Plasmid

   #219421

   Purpose

   Expresses 3873 bp of mPer1 coding sequence in E. coli.

   Depositor

   Aziz Sancar

   Article

   Cao et al Proc Natl Acad Sci U S A. 2023 Oct 24;12

   Insert

   mPer1 (Per1 Mouse)
   

   Tags

   His and V5

   Expression

   Bacterial and Mammalian

   Available Since

   Dec. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. UMG-SP-3_pET29b

   Plasmid

   #215435

   Purpose

   Expression construct for UMG-SP-3 gene, codon optimized for expression in E. coli

   Depositor

   Gregg Beckham

   Article

   Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

   Insert

   UMG-SP-3
   

   Expression

   Bacterial

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. NfPolyA_pET21b

   Plasmid

   #215413

   Purpose

   Expression construct for NfPolyA gene, codon optimized for expression in E. coli

   Depositor

   Gregg Beckham

   Article

   Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

   Insert

   NfPolyA
   

   Expression

   Bacterial

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. B-gTEMP/pRSET-b

   Plasmid

   #188446

   Purpose

   B-gTEMP for bacterial expression

   Depositor

   Takeharu Nagai

   Article

   Lu et al Nano Lett. 2022 Jul 27;22(14):5698-5707.

   Insert

   B-gTEMP
   

   Tags

   6xHis-tag

   Expression

   Bacterial

   Available Since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET22b-ELP[V]60

   Plasmid

   #219406

   Purpose

   (VPGVG)60

   Depositor

   Danielle Mai

   Article

   Wirtz et al Biomacromolecules. 2024 Jul 9. doi: 10.1

   Insert

   ELP[V]60
   

   Tags

   6xHis

   Expression

   Bacterial

   Available Since

   July 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET-mKelly1

   Plasmid

   #119782

   Purpose

   Expresses mKelly1 tagged N-terminally with an MHHHHHHGSG tag

   Depositor

   Stephen Mayo

   Article

   Wannier et al Proc Natl Acad Sci U S A. 2018 Nov 27;11

   Insert

   mKelly1
   

   Tags

   6xHis tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDF0118 DisCas7-11 bacterial expression with Twinstrep-SUMO-Codon

   Plasmid

   #172503

   Purpose

   Encodes the DisCas7-11 protein only for bacterial recombinant protein expression for purification

   Depositor

   Omar Abudayyeh, Jonathan Gootenberg

   Article

   Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

   Insert

   DisCas7-11 bacterial expression with Twinstrep-SUMO-Codon
   

   Expression

   Bacterial

   Available Since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. 3xFLAG-dCas9/p-bacteria

   Plasmid

   #64325

   Purpose

   Expresses 3xFLAG-dCas9 in E. coli in a tetracycline-dependent manner for enChIP analysis to purify specific genomic regions of interest.

   Depositor

   Hodaka Fujii

   Article

   Fujita et al BMC Res Notes. 2018 Jun 14;11(1):387. do

   Insert

   3xFLAG-dCas9
   

   Use

   CRISPR; Tetracycline-inducible

   Tags

   3xFLAG

   Expression

   Bacterial

   Mutation

   D10A, H840A

   Promoter

   pLtetO-1

   Available Since

   Aug. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET14-GFP15_Encap

   Plasmid

   #86407

   Purpose

   Expresses sfGFP tagged with a 15 amino acid CLP and encapsulin as an operon

   Depositor

   David Savage

   Article

   Cassidy-Amstutz et al Biochemistry. 2016 Jun 21;55(24):3461-8.

   Inserts

   sfGFP with a 15aa CLP tag
   Encapsulin
   

   Tags

   6x Histidine

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFA6a-mEOS3.2-KanMX6

    Plasmid

    #87030

    Purpose

    Tag S. pombe genes in C terminal with mEOS3.2

    Depositor

    Julien Berro, Thomas Pollard

    Article

    Laplante et al Proc Natl Acad Sci U S A. 2016 Oct 4;113

    Insert

    mEOS3.2
    

    Expression

    Bacterial and Yeast

    Available Since

    Feb. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEB1-moxGFP

    Plasmid

    #103984

    Purpose

    Plasmid encoding moxGFP

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    moxGFP
    

    Use

    Low copy

    Expression

    Bacterial

    Mutation

    Mutations relative to wild-type GFP (S30R, Y39N, …

    Promoter

    proC

    Available Since

    Dec. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMRE130

    Plasmid

    #118484

    Purpose

    broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    TagBFP2
    

    Expression

    Bacterial

    Available Since

    July 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. SS9_RNA

    Plasmid

    #71656

    Purpose

    SS9-targeting gRNA for co-transformation with pSS9

    Depositor

    Ryan Gill

    Article

    Bassalo et al ACS Synth Biol. 2016 Apr 22.

    Insert

    SS9 gRNA
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    J23119

    Available Since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pdestDD04Neo

    Plasmid

    #26386

    Depositor

    Denis Dupuy

    Article

    Giordano-Santini et al Nat Methods. 2010 Sep;7(9):721-3. doi: 1

    Type

    Empty backbone

    Expression

    Bacterial and Worm

    Available Since

    Oct. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCS2+ centrin-mKate2

    Plasmid

    #105941

    Purpose

    in vitro transcription, zf centrin N-terminally tagged with mkate2

    Depositor

    Caren Norden

    Article

    Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

    Insert

    Centrin
    

    Tags

    mKate2

    Expression

    Bacterial

    Available Since

    Feb. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBad-UGAcS

    Plasmid

    #172137

    Purpose

    bacteria expression plasmid for a UDP-GlcNAc sensor

    Depositor

    Huiwang Ai

    Article

    Li et al ACS Cent Sci. 2021 Oct 27;7(10):1763-177

    Insert

    UGAcS
    

    Expression

    Bacterial

    Mutation

    H605Q- please see depositor comments

    Promoter

    araBAD

    Available Since

    Aug. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pQE81XN-mGold

    Plasmid

    #218959

    Purpose

    Expresses Hisx6-Tagged mGold in bacteria

    Depositor

    Benjamin Glick

    Insert

    mGold
    

    Tags

    6X-His

    Expression

    Bacterial

    Promoter

    T5

    Available Since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSb_init containing anti SARS-CoV-2_RBD sybody Sb#45

    Plasmid

    #153526

    Purpose

    Expression of the anti SARS-CoV-2_RBD sybody Sb#45

    Depositor

    Markus Seeger

    Article

    Walter et al bioRxiv 2020.04.16.045419

    Insert

    Sb#45
    

    Expression

    Bacterial

    Promoter

    pBAD

    Available Since

    June 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET-Duet1-His-TEV-mCherry-p62

    Plasmid

    #199780

    Purpose

    Used for the expression of mCherry labelled p62 (SMC Internal No. 391).

    Depositor

    Sascha Martens

    Article

    Wurzer et al Elife. 2015 Sep 28;4:e08941. doi: 10.755

    Insert

    p62 (NUP62 Human)
    

    Tags

    6x His, TEV, mCherry

    Expression

    Bacterial

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pST-K

    Plasmid

    #44560

    Purpose

    for ectopic constitutive expression of mycobacterial proteins

    Depositor

    Vinay Nandicoori

    Article

    Parikh et al Appl Environ Microbiol. 2013 Mar;79(5):1

    Type

    Empty backbone

    Use

    E.coli-mycobacteria shuttle vector

    Tags

    Flag tag and His tag

    Expression

    Bacterial

    Promoter

    UV15 promoter

    Available Since

    Oct. 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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